Restriction map for
pFN21AB9895
(Circular) (Six-base) MAPSORT of: pFN21AB9895.seq Check: 4758 from: 1 to: 5416
pFN21AB9895 5416 bp
With 182 enzymes: SgfI *
February 12, 2011 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3226 278
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 1937
Size: 822 4594
Acc65I G'GTAC_C
Cuts at: 2737 2737
Size: 5416
AclI AA'CG_TT
Cuts at: 1563 3543 3916 1563
Size: 1980 373 3063
Fragments arranged by size:
3063 1980 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2641 3479 4863 5131 1632
Size: 473 536 838 1384 268 1917
Fragments arranged by size:
1917 1384 838 536 473 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 4315 5292 1203
Size: 3112 977 1327
Fragments arranged by size:
3112 1327 977
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 2540 4145 2540
Size: 1605 3811
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5416
AlwNI CAG_nnn'CTG
Cuts at: 2083 3159 4731 2083
Size: 1076 1572 2768
Fragments arranged by size:
2768 1572 1076
ApaI G_GGCC'C
Cuts at: 2306 2306
Size: 5416
ApaLI G'TGCA_C
Cuts at: 2397 3473 4629 2397
Size: 1076 1156 3184
Fragments arranged by size:
3184 1156 1076
ApoI r'AATT_y
Cuts at: 1493 2048 2725 2951 3103 5212 1493
Size: 555 677 226 152 2109 1697
Fragments arranged by size:
2109 1697 677 555 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3970 160
Size: 872 2938 1606
Fragments arranged by size:
2938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2741 1870
Size: 73 798 4545
Fragments arranged by size:
4545 798 73
BamHI G'GATC_C
Cuts at: 2498 2746 3113 2498
Size: 248 367 4801
Fragments arranged by size:
4801 367 248
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2412 2737 4192 618
Size: 325 217 627 625 325 1455 1842
Fragments arranged by size:
1842 1455 627 625 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2195 2306 2735 1391
Size: 804 111 429 4072
Fragments arranged by size:
4072 804 429 111
BbeI G_GCGC'C
Cuts at: 2416 2416
Size: 5416
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 2647 928
Size: 971 567 181 3697
Fragments arranged by size:
3697 971 567 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3607 3641 1903
Size: 34 1670 34 3678
Fragments arranged by size:
3678 1670 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3308 4518 3308
Size: 1210 4206
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4027 136
Size: 107 122 71 631 605 2355 1525
Fragments arranged by size:
2355 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2833 2833
Size: 5416
Bme1580I G_kGCm'C
Cuts at: 1790 2306 2366 2401 3477 4633 1790
Size: 516 60 35 1076 1156 2573
Fragments arranged by size:
2573 1156 1076 516 60 35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5416
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2267 3147 4105
Size: 416 53 83 399 24 850 880 958
Cuts at: 4105 442
Size: 1753
Fragments arranged by size:
1753 958 880 850 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 4076 1239
Size: 1094 1743 2579
Fragments arranged by size:
2579 1743 1094
Bpu10I CC'TnA_GC
Cuts at: 2456 2456
Size: 5416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4406 4704 3541
Size: 865 298 4253
Fragments arranged by size:
4253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 882
Size: 617 2580 2219
Fragments arranged by size:
2580 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3207 493
Size: 711 2003 2702
Fragments arranged by size:
2702 2003 711
BsaBI GATnn'nnATC
Cuts at: 5410 5410
Size: 5416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2413 3223 3605 4240
Size: 53 83 186 936 880 810 382 635
Cuts at: 4240 275
Size: 1451
Fragments arranged by size:
1451 936 880 810 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5191
Fragments arranged by size:
5191 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5416
BseYI C'CCAG_C
Cuts at: 2336 4619 5115 2336
Size: 2283 496 2637
Fragments arranged by size:
2637 2283 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4843
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2866 3627 4655 1540
Size: 275 186 865 761 1028 2301
Fragments arranged by size:
2301 1028 865 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2401 3477 3562 4633 2401
Size: 1076 85 1071 3184
Fragments arranged by size:
3184 1076 1071 85
BsmI GAATG_Cn'
Cuts at: 2279 2927 3020 2279
Size: 648 93 4675
Fragments arranged by size:
4675 648 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2306 2366 2401 2735 3477 3562
Size: 399 405 111 60 35 334 742 85
Cuts at: 3562 4633 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 334 111 85
60 35
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 2667 3305 5035 2667
Size: 638 1730 3048
Fragments arranged by size:
3048 1730 638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2390 2771 844
Size: 1546 381 3489
Fragments arranged by size:
3489 1546 381
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 1394
Size: 429 1480 3507
Fragments arranged by size:
3507 1480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2376 3911 4085 65
Size: 1183 1128 1535 174 1396
Fragments arranged by size:
1535 1396 1183 1128 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4060 4247 1700
Size: 110 2250 187 2869
Fragments arranged by size:
2869 2250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5416
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5416
BssSI C'ACGA_G
Cuts at: 3476 4488 3476
Size: 1012 4404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2105 2405 794
Size: 1311 300 3805
Fragments arranged by size:
3805 1311 300
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5416
BstYI r'GATC_y
Cuts at: 1972 2498 2746 3113 3498 3515 4967 5053 5065
Size: 526 248 367 385 17 1452 86 12
Cuts at: 5065 5411 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 526 385 367 346 248 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4864
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4677
Fragments arranged by size:
4677 683 56
BtsI GCAGTG_nn'
Cuts at: 2349 2620 2932 3726 3746 2349
Size: 271 312 794 20 4019
Fragments arranged by size:
4019 794 312 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2046 2720 2046
Size: 674 4742
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2679 2863 3752
Size: 54 1425 50 275 18 849 184 889
Cuts at: 3752 5381 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 849 275 184 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2863 1537
Size: 275 1051 4090
Fragments arranged by size:
4090 1051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 3346 1353
Size: 1286 707 3423
Fragments arranged by size:
3423 1286 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4003 4377 4523 387
Size: 1064 138 2414 374 146 1280
Fragments arranged by size:
2414 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2333 2641 3479 4076 4863 5131
Size: 393 473 228 308 838 597 787 268
Cuts at: 5131 1239
Size: 1524
Fragments arranged by size:
1524 838 787 597 473 393 308 268
228
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 5416
EcoO109I rG'GnC_Cy
Cuts at: 2136 2303 2136
Size: 167 5249
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5416
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 2416 4563 1678
Size: 738 2147 2531
Fragments arranged by size:
2531 2147 738
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2526 2558 4057 4089 4131
Size: 32 135 32 954 32 1499 32 42
Cuts at: 4131 4163 1373
Size: 32 2626
Fragments arranged by size:
2626 1499 954 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2070 2332 2760 3006 669
Size: 988 281 132 262 428 246 3079
Fragments arranged by size:
3079 988 428 281 262 246 132
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KasI G'GCGC_C
Cuts at: 2412 2412
Size: 5416
KpnI G_GTAC'C
Cuts at: 2741 2741
Size: 5416
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4530 4714 1305
Size: 3225 184 2007
Fragments arranged by size:
3225 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2681 10
Size: 54 1425 343 849 2745
Fragments arranged by size:
2745 1425 849 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2235 2369 3374 3733 3892 518
Size: 648 1069 134 1005 359 159 2042
Fragments arranged by size:
2042 1069 1005 648 359 159 134
MspA1I CmG'CkG
Cuts at: 1606 2832 3509 4657 4902 1606
Size: 1226 677 1148 245 2120
Fragments arranged by size:
2120 1226 1148 677 245
NarI GG'CG_CC
Cuts at: 2413 2413
Size: 5416
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4864
NdeI CA'TA_TG
Cuts at: 387 2148 387
Size: 1761 3655
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2863 2863
Size: 5416
NspI r_CATG'y
Cuts at: 1132 1229 2774 4319 1132
Size: 97 1545 1545 2229
Fragments arranged by size:
2229 1545 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2479 1348
Size: 1131 4285
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5416
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 4998 5030 3485
Size: 32 1481 32 3871
Fragments arranged by size:
3871 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2986 2986
Size: 5416
PspOMI G'GGCC_C
Cuts at: 2302 2302
Size: 5416
PstI C_TGCA'G
Cuts at: 830 2134 2768 830
Size: 1304 634 3478
Fragments arranged by size:
3478 1304 634
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SbfI CC_TGCA'GG
Cuts at: 2768 2768
Size: 5416
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2130 2764 3899 4580 4771 826
Size: 219 1085 634 1135 681 191 1471
Fragments arranged by size:
1471 1135 1085 681 634 219 191
SfoI GGC'GCC
Cuts at: 2414 2414
Size: 5416
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2743 1872
Size: 871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3520 4421 4683 820
Size: 197 926 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5416
SphI G_CATG'C
Cuts at: 1132 2774 1132
Size: 1642 3774
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5416
StyI C'CwwG_G
Cuts at: 513 1065 2187 2855 513
Size: 552 1122 668 3074
Fragments arranged by size:
3074 1122 668 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3443 3460 3613 3798 4243 325
Size: 1255 1863 17 153 185 445 1498
Fragments arranged by size:
1863 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3662 5374 96
Size: 274 80 33 51 1443 1685 1712 138
Fragments arranged by size:
1712 1685 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5376 5408 5376
Size: 32 5384
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2356
Size: 5416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5416
XmaI C'CCGG_G
Cuts at: 1870 2741 1870
Size: 871 4545
XmnI GAAnn'nnTTC
Cuts at: 2489 3545 2489
Size: 1056 4360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3224 276
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI