Restriction map for
pFN21AB9894
(Circular) (Six-base) MAPSORT of: pFN21AB9894.seq Check: 9288 from: 1 to: 15688
pFN21AB9894 15688 bp
With 182 enzymes: SgfI *
December 22, 2010 15:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 10460 11038 11786 10460
Size: 578 748 14362
Fragments arranged by size:
14362 748 578
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 13498 278
Size: 53 83 186 936 11962 2468
Fragments arranged by size:
11962 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3751 3816 4351 6200 10556 11349 12155 13031
Size: 1814 65 535 1849 4356 793 806 876
Cuts at: 13031 1937
Size: 4594
Fragments arranged by size:
4594 4356 1849 1814 876 806 793 535
65
Acc65I G'GTAC_C
Cuts at: 3482 7592 11114 13009 3482
Size: 4110 3522 1895 6161
Fragments arranged by size:
6161 4110 3522 1895
AclI AA'CG_TT
Cuts at: 1563 10243 13815 14188 1563
Size: 8680 3572 373 3063
Fragments arranged by size:
8680 3572 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3235 4742 5283 5367 5843 6121 6813
Size: 977 626 1507 541 84 476 278 692
Cuts at: 6813 6864 6978 7058 7203 7524 7608 8189 9591
Size: 51 114 80 145 321 84 581 1402
Cuts at: 9591 10490 10550 10709 11086 13751 15135 15403 1632
Size: 899 60 159 377 2665 1384 268 1917
Fragments arranged by size:
2665 1917 1507 1402 1384 977 899 692
626 581 541 476 377 321 278 268
159 145 114 84 84 80 60 51
AflII C'TTAA_G
Cuts at: 820 1017 4157 10735 820
Size: 197 3140 6578 5773
Fragments arranged by size:
6578 5773 3140 197
AflIII A'CryG_T
Cuts at: 1203 5832 7926 10638 12457 14587 15564 1203
Size: 4629 2094 2712 1819 2130 977 1327
Fragments arranged by size:
4629 2712 2130 2094 1819 1327 977
AgeI A'CCGG_T
Cuts at: 14519 14519
Size: 15688
AhdI GACnn_n'nnGTC
Cuts at: 14417 14417
Size: 15688
AleI CACnn'nnGTG
Cuts at: 1166 5391 1166
Size: 4225 11463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5596 8970 9951 1184
Size: 4412 3374 981 6921
Fragments arranged by size:
6921 4412 3374 981
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4646 4678 6231 6263 6915 6947 9039 9071 11424
Size: 32 1553 32 652 32 2092 32 2353
Cuts at: 11424 11456 4646
Size: 32 8878
Fragments arranged by size:
8878 2353 2092 1553 652 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 3097 4681 4733 4963 7423 10468 11109 12047 12223
Size: 1584 52 230 2460 3045 641 938 176
Cuts at: 12223 12746 12905 13431 15003 3097
Size: 523 159 526 1572 3782
Fragments arranged by size:
3782 3045 2460 1584 1572 938 641 526
523 230 176 159 52
ApaI G_GGCC'C
Cuts at: 6447 6447
Size: 15688
ApaLI G'TGCA_C
Cuts at: 3950 4424 13745 14901 3950
Size: 474 9321 1156 4737
Fragments arranged by size:
9321 4737 1156 474
ApoI r'AATT_y
Cuts at: 1493 2188 2525 2720 3508 3827 4280 4515 4524
Size: 695 337 195 788 319 453 235 9
Cuts at: 4524 5726 6293 7287 7634 7944 9680 10576 10582
Size: 1202 567 994 347 310 1736 896 6
Cuts at: 10582 10962 12997 13223 13375 15484 1493
Size: 380 2035 226 152 2109 1697
Fragments arranged by size:
2109 2035 1736 1697 1202 994 896 788
695 567 453 380 347 337 319 310
235 226 195 152 9 6
AseI AT'TA_AT
Cuts at: 160 1032 4982 8544 10823 14242 160
Size: 872 3950 3562 2279 3419 1606
Fragments arranged by size:
3950 3562 3419 2279 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15688
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2475 10398 13013 1870
Size: 73 77 455 7923 2615 4545
Fragments arranged by size:
7923 4545 2615 455 77 73
AvrII C'CTAG_G
Cuts at: 7310 7310
Size: 15688
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2214 2247 6800 6833 2214
Size: 33 4553 33 11069
Fragments arranged by size:
11069 4553 33 33
BamHI G'GATC_C
Cuts at: 3406 5845 6597 13018 13385 3406
Size: 2439 752 6421 367 5709
Fragments arranged by size:
6421 5709 2439 752 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 3482 6838 7592 10486
Size: 325 217 627 270 1425 3356 754 2894
Cuts at: 10486 11114 11384 11801 13009 14464 618
Size: 628 270 417 1208 1455 1842
Fragments arranged by size:
3356 2894 1842 1455 1425 1208 754 628
627 417 325 270 270 217
BanII G_rGCy'C
Cuts at: 1391 2020 4813 6447 6652 7899 8904 10017 10053
Size: 629 2793 1634 205 1247 1005 1113 36
Cuts at: 10053 13007 1391
Size: 2954 4072
Fragments arranged by size:
4072 2954 2793 1634 1247 1113 1005 629
205 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2487 3108 4730 6819 7098 7773 8177
Size: 971 588 621 1622 2089 279 675 404
Cuts at: 8177 10004 11235 12656 928
Size: 1827 1231 1421 3960
Fragments arranged by size:
3960 2089 1827 1622 1421 1231 971 675
621 588 404 279
BbvCI CC'TCA_GC
Cuts at: 2327 3120 3128 2327
Size: 793 8 14887
Fragments arranged by size:
14887 793 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2360 2394 4988 5022 5588 5622 13879
Size: 34 423 34 2594 34 566 34 8257
Cuts at: 13879 13913 1903
Size: 34 3678
Fragments arranged by size:
8257 3678 2594 566 423 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2860 2947 6756 7587 8981 9966 13580 14790
Size: 784 87 3809 831 1394 985 3614 1210
Cuts at: 14790 2076
Size: 2974
Fragments arranged by size:
3809 3614 2974 1394 1210 985 831 784
87
BclI T'GATC_A
Cuts at: 1548 4030 4876 1548
Size: 2482 846 12360
Fragments arranged by size:
12360 2482 846
BfrBI ATG'CAT
Cuts at: 4994 5015 4994
Size: 21 15667
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5603 6878 14299
Size: 107 122 71 631 605 3931 1275 7421
Cuts at: 14299 136
Size: 1525
Fragments arranged by size:
7421 3931 1525 1275 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2697 8960 11620 15683 2697
Size: 6263 2660 4063 2702
Fragments arranged by size:
6263 4063 2702 2660
BlpI GC'TnA_GC
Cuts at: 2033 3662 6651 8801 12957 13105 2033
Size: 1629 2989 2150 4156 148 4616
Fragments arranged by size:
4616 4156 2989 2150 1629 148
Bme1580I G_kGCm'C
Cuts at: 1790 3730 3856 3954 4428 6447 13749 14905 1790
Size: 1940 126 98 474 2019 7302 1156 2573
Fragments arranged by size:
7302 2573 2019 1940 1156 474 126 98
BmgBI CAC'GTC
Cuts at: 1318 2599 6172 1318
Size: 1281 3573 10834
Fragments arranged by size:
10834 3573 1281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4811 9161 12807
Size: 416 53 83 399 24 3394 4350 3646
Cuts at: 12807 13419 14377 442
Size: 612 958 1753
Fragments arranged by size:
4350 3646 3394 1753 958 612 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 11756 1056
Size: 10700 4988
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12838 12838
Size: 15688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3482 4994 5407 5808 7445 8495 9066 9183
Size: 2243 1512 413 401 1637 1050 571 117
Cuts at: 9183 9266 9563 9881 9938 10392 12455 14348 1239
Size: 83 297 318 57 454 2063 1893 2579
Fragments arranged by size:
2579 2243 2063 1893 1637 1512 1050 571
454 413 401 318 297 117 83 57
Bpu10I CC'TnA_GC
Cuts at: 2327 3120 3128 4493 5449 9491 11711 12303 2327
Size: 793 8 1365 956 4042 2220 592 5712
Fragments arranged by size:
5712 4042 2220 1365 956 793 592 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4797 7536 10896 10998 11346 11771 13813 14678 14976
Size: 2739 3360 102 348 425 2042 865 298
Cuts at: 14976 4797
Size: 5509
Fragments arranged by size:
5509 3360 2739 2042 865 425 348 298
102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2031 2484 9221 12780 14351 882
Size: 617 532 453 6737 3559 1571 2219
Fragments arranged by size:
6737 3559 2219 1571 617 532 453
BsaAI yAC'GTr
Cuts at: 493 1204 9796 13479 493
Size: 711 8592 3683 2702
Fragments arranged by size:
8592 3683 2702 711
BsaBI GATnn'nnATC
Cuts at: 8484 10988 11037 12879 15682 8484
Size: 2504 49 1842 2803 8490
Fragments arranged by size:
8490 2803 2504 1842 49
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7129 13495 13877 14512
Size: 53 83 186 936 5596 6366 382 635
Cuts at: 14512 275
Size: 1451
Fragments arranged by size:
6366 5596 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5842 12630 14121 14519 14793 14940 15351 5842
Size: 6788 1491 398 274 147 411 6179
Fragments arranged by size:
6788 6179 1491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4701 4731 5011 5041 6915
Size: 30 165 30 3298 30 280 30 1874
Cuts at: 6915 6945 8077 8107 9039 9069 1178
Size: 30 1132 30 932 30 7797
Fragments arranged by size:
7797 3298 1874 1132 932 280 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2744 3162 3247 8092 8712 1186
Size: 1558 418 85 4845 620 8162
Fragments arranged by size:
8162 4845 1558 620 418 85
BseYI C'CCAG_C
Cuts at: 5459 6361 6876 7903 9296 10083 14891 15387 5459
Size: 902 515 1027 1393 787 4808 496 5760
Fragments arranged by size:
5760 4808 1393 1027 902 787 515 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3186 4749 5633 6363 6367 7437 10377
Size: 573 1450 1563 884 730 4 1070 2940
Cuts at: 10377 11344 11742 1163
Size: 967 398 5109
Fragments arranged by size:
5109 2940 1563 1450 1070 967 884 730
573 398 4
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7043 13138 13899 14927 1540
Size: 275 186 5042 6095 761 1028 2301
Fragments arranged by size:
6095 5042 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3663 3954 3999 4428 4813 5445 6652 7346 7431
Size: 291 45 429 385 632 1207 694 85
Cuts at: 7431 7887 7899 8251 8904 9621 9880 9906 10017
Size: 456 12 352 653 717 259 26 111
Cuts at: 10017 10053 11341 12249 13749 13834 14905 3663
Size: 36 1288 908 1500 85 1071 4446
Fragments arranged by size:
4446 1500 1288 1207 1071 908 717 694
653 632 456 429 385 352 291 259
111 85 85 45 36 26 12
BsmI GAATG_Cn'
Cuts at: 2901 9713 9826 10892 11255 12682 13199 13292 2901
Size: 6812 113 1066 363 1427 517 93 5297
Fragments arranged by size:
6812 5297 1427 1066 517 363 113 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7933 7933
Size: 15688
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 3663 3730 3856 3954 3999 4428
Size: 399 230 1643 67 126 98 45 429
Cuts at: 4428 4813 5445 6447 6652 7346 7431 7887 7899
Size: 385 632 1002 205 694 85 456 12
Cuts at: 7899 8251 8904 9621 9880 9906 10017 10053 11341
Size: 352 653 717 259 26 111 36 1288
Cuts at: 11341 12249 13007 13749 13834 14905 1391
Size: 908 758 742 85 1071 2174
Fragments arranged by size:
2174 1643 1288 1071 1002 908 758 742
717 694 653 632 456 429 399 385
352 259 230 205 126 111 98 85
85 67 45 36 26 12
BspEI T'CCGG_A
Cuts at: 5842 12630 15351 5842
Size: 6788 2721 6179
Fragments arranged by size:
6788 6179 2721
BspHI T'CATG_A
Cuts at: 5106 8019 8110 10264 13577 15307 5106
Size: 2913 91 2154 3313 1730 5487
Fragments arranged by size:
5487 3313 2913 2154 1730 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3007 9187 10460 11038 11786 13043 844
Size: 2163 6180 1273 578 748 1257 3489
Fragments arranged by size:
6180 3489 2163 1273 1257 748 578
BsrBI CCG'CTC
Cuts at: 1394 1823 10440 13575 1394
Size: 429 8617 3135 3507
Fragments arranged by size:
8617 3507 3135 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6510 7370 11355 14183 14357 65
Size: 1183 5262 860 3985 2828 174 1396
Fragments arranged by size:
5262 3985 2828 1396 1183 860 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7042 11043 14332 14519 1700
Size: 110 5232 4001 3289 187 2869
Fragments arranged by size:
5232 4001 3289 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2590 3303 7233 11179 96
Size: 2494 713 3930 3946 4605
Fragments arranged by size:
4605 3946 3930 2494 713
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 15688
BssSI C'ACGA_G
Cuts at: 4183 7391 7856 13748 14760 4183
Size: 3208 465 5892 1012 5111
Fragments arranged by size:
5892 5111 3208 1012 465
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6296 11801 794
Size: 5502 5505 4681
Fragments arranged by size:
5505 5502 4681
BstBI TT'CG_AA
Cuts at: 4846 9124 10580 12445 14492 4846
Size: 4278 1456 1865 2047 6042
Fragments arranged by size:
6042 4278 2047 1865 1456
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 15688
BstXI CCAn_nnnn'nTGG
Cuts at: 3479 5227 3479
Size: 1748 13940
BstYI r'GATC_y
Cuts at: 1972 2282 2697 3406 5845 6597 8960 10283 11620
Size: 310 415 709 2439 752 2363 1323 1337
Cuts at: 11620 13018 13385 13770 13787 15239 15325 15337 15683
Size: 1398 367 385 17 1452 86 12 346
Cuts at: 15683 1972
Size: 1977
Fragments arranged by size:
2439 2363 1977 1452 1398 1337 1323 752
709 415 385 367 346 310 86 17
12
BstZ17I GTA'TAC
Cuts at: 6201 10557 6201
Size: 4356 11332
Bsu36I CC'TnA_GG
Cuts at: 3762 5544 3762
Size: 1782 13906
BtgI C'CryG_G
Cuts at: 513 1065 2069 4921 6179 7151 7457 7833 8294
Size: 552 1004 2852 1258 972 306 376 461
Cuts at: 8294 9907 9942 10515 11666 513
Size: 1613 35 573 1151 4535
Fragments arranged by size:
4535 2852 1613 1258 1151 1004 972 573
552 461 376 306 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5977 12013 487
Size: 683 56 4751 6036 4162
Fragments arranged by size:
6036 4751 4162 683 56
BtsI GCAGTG_nn'
Cuts at: 3663 4751 5145 6355 6375 7255 7449 7641 10113
Size: 1088 394 1210 20 880 194 192 2472
Cuts at: 10113 10481 11505 12837 13204 13998 14018 3663
Size: 368 1024 1332 367 794 20 5333
Fragments arranged by size:
5333 2472 1332 1210 1088 1024 880 794
394 368 367 194 192 20 20
ClaI AT'CG_AT
Cuts at: 1554 2423 10785 1554
Size: 869 8362 6457
Fragments arranged by size:
8362 6457 869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 15653
DraI TTT'AAA
Cuts at: 2130 5916 6403 8218 12992 2130
Size: 3786 487 1815 4774 4826
Fragments arranged by size:
4826 4774 3786 1815 487
DraIII CAC_nnn'GTG
Cuts at: 7333 9155 10486 11322 7333
Size: 1822 1331 836 11699
Fragments arranged by size:
11699 1822 1331 836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 14695 809
Size: 507 13379 1802
Fragments arranged by size:
13379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6870 7040 7365
Size: 54 1425 50 275 18 5040 170 325
Cuts at: 7365 9328 10873 11238 12643 13135 14024 15653 8
Size: 1963 1545 365 1405 492 889 1629 43
Fragments arranged by size:
5040 1963 1629 1545 1425 1405 889 492
365 325 275 170 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7040 13135 1537
Size: 275 5228 6095 4090
Fragments arranged by size:
6095 5228 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3087 4269 4372 5542 6140 6735 6990 7964
Size: 1734 1182 103 1170 598 595 255 974
Cuts at: 7964 7998 8334 8441 8838 8853 10005 10728 11052
Size: 34 336 107 397 15 1152 723 324
Cuts at: 11052 12839 13618 1353
Size: 1787 779 3423
Fragments arranged by size:
3423 1787 1734 1182 1170 1152 974 779
723 598 595 397 336 324 255 107
103 34 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8394 14275 14649 14795 387
Size: 1064 138 6805 5881 374 146 1280
Fragments arranged by size:
6805 5881 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4811 6650 7897 8902 10015 10051 4811
Size: 1839 1247 1005 1113 36 10448
Fragments arranged by size:
10448 1839 1247 1113 1005 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 3235 3482 4742 4994 5283 5367
Size: 393 977 626 247 1260 252 289 84
Cuts at: 5367 5407 5808 5843 6121 6813 6864 6978 7058
Size: 40 401 35 278 692 51 114 80
Cuts at: 7058 7203 7445 7524 7608 8189 8495 9066 9183
Size: 145 242 79 84 581 306 571 117
Cuts at: 9183 9266 9563 9591 9881 9938 10392 10490 10550
Size: 83 297 28 290 57 454 98 60
Cuts at: 10550 10709 11086 12455 13751 14348 15135 15403 1239
Size: 159 377 1369 1296 597 787 268 1524
Fragments arranged by size:
1524 1369 1296 1260 977 787 692 626
597 581 571 454 401 393 377 306
297 290 289 278 268 252 247 242
159 145 117 114 98 84 84 83
80 79 60 57 51 40 35 28
EcoNI CCTnn'n_nnAGG
Cuts at: 4175 5195 4175
Size: 1020 14668
EcoO109I rG'GnC_Cy
Cuts at: 3124 3266 6443 3124
Size: 142 3177 12369
Fragments arranged by size:
12369 3177 142
EcoRI G'AATT_C
Cuts at: 7944 10576 12997 13375 7944
Size: 2632 2421 378 10257
Fragments arranged by size:
10257 2632 2421 378
EcoRV GAT'ATC
Cuts at: 7140 10504 7140
Size: 3364 12324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2824 4187 10277 11116 11542 11642 2824
Size: 1363 6090 839 426 100 6870
Fragments arranged by size:
6870 6090 1363 839 426 100
FspI TGC'GCA
Cuts at: 7871 14194 7871
Size: 6323 9365
HaeII r_GCGC'y
Cuts at: 1678 3905 9898 14835 1678
Size: 2227 5993 4937 2531
Fragments arranged by size:
5993 4937 2531 2227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 3922 3954 5088
Size: 32 135 32 521 32 1797 32 1134
Cuts at: 5088 5120 5757 5789 6749 6781 7799 7831 8343
Size: 32 637 32 960 32 1018 32 512
Cuts at: 8343 8375 8891 8923 12653 12685 14329 14361 14403
Size: 32 516 32 3730 32 1644 32 42
Cuts at: 14403 14435 1373
Size: 32 2626
Fragments arranged by size:
3730 2626 1797 1644 1134 1018 960 637
521 516 512 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 3363 4701 6495 6907 12141
Size: 988 281 397 1028 1338 1794 412 5234
Cuts at: 12141 12916 13032 13278 669
Size: 775 116 246 3079
Fragments arranged by size:
5234 3079 1794 1338 1028 988 775 412
397 281 246 116
HindIII A'AGCT_T
Cuts at: 748 6144 9493 9745 748
Size: 5396 3349 252 6691
Fragments arranged by size:
6691 5396 3349 252
HpaI GTT'AAC
Cuts at: 2335 13278 2335
Size: 10943 4745
KpnI G_GTAC'C
Cuts at: 3486 7596 11118 13013 3486
Size: 4110 3522 1895 6161
Fragments arranged by size:
6161 4110 3522 1895
MfeI C'AATT_G
Cuts at: 2567 3864 4645 4830 5592 5879 13287 2567
Size: 1297 781 185 762 287 7408 4968
Fragments arranged by size:
7408 4968 1297 781 762 287 185
MluI A'CGCG_T
Cuts at: 15564 15564
Size: 15688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2285 2953 3935 4632 5284 6204 6418 6766
Size: 980 668 982 697 652 920 214 348
Cuts at: 6766 7676 8965 9447 10852 12611 12953 14802 14986
Size: 910 1289 482 1405 1759 342 1849 184
Cuts at: 14986 1305
Size: 2007
Fragments arranged by size:
2007 1849 1759 1405 1289 982 980 920
910 697 668 652 482 348 342 214
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6872 7367 9330 10875 11240
Size: 54 1425 343 5040 495 1963 1545 365
Cuts at: 11240 12645 10
Size: 1405 3053
Fragments arranged by size:
5040 3053 1963 1545 1425 1405 495 365
343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5225 5391 5578 7358 7404 7929 8490
Size: 648 4059 166 187 1780 46 525 561
Cuts at: 8490 8978 9770 9933 10124 10269 10615 13646 14005
Size: 488 792 163 191 145 346 3031 359
Cuts at: 14005 14164 518
Size: 159 2042
Fragments arranged by size:
4059 3031 2042 1780 792 648 561 525
488 359 346 191 187 166 163 159
145 46
MspA1I CmG'CkG
Cuts at: 1606 2040 2071 2195 3368 4220 6064 6361 6631
Size: 434 31 124 1173 852 1844 297 270
Cuts at: 6631 6876 8090 8205 9859 10381 10871 11264 11464
Size: 245 1214 115 1654 522 490 393 200
Cuts at: 11464 11578 11587 12716 12961 13104 13781 14929 15174
Size: 114 9 1129 245 143 677 1148 245
Cuts at: 15174 1606
Size: 2120
Fragments arranged by size:
2120 1844 1654 1214 1173 1148 1129 852
677 522 490 434 393 297 270 245
245 245 200 143 124 115 114 31
9
NaeI GCC'GGC
Cuts at: 7044 7044
Size: 15688
NcoI C'CATG_G
Cuts at: 513 1065 4921 6179 7151 7457 7833 8294 10515
Size: 552 3856 1258 972 306 376 461 2221
Cuts at: 10515 513
Size: 5686
Fragments arranged by size:
5686 3856 2221 1258 972 552 461 376
306
NdeI CA'TA_TG
Cuts at: 387 3115 3286 3445 4754 387
Size: 2728 171 159 1309 11321
Fragments arranged by size:
11321 2728 1309 171 159
NgoMIV G'CCGG_C
Cuts at: 7042 7042
Size: 15688
NheI G'CTAG_C
Cuts at: 1052 11752 1052
Size: 10700 4988
NotI GC'GGCC_GC
Cuts at: 13135 13135
Size: 15688
NsiI A_TGCA'T
Cuts at: 4996 5017 4996
Size: 21 15667
NspI r_CATG'y
Cuts at: 1132 1229 4344 4358 5577 5836 6503 7403 7930
Size: 97 3115 14 1219 259 667 900 527
Cuts at: 7930 8075 10775 10808 10906 13046 14591 1132
Size: 145 2700 33 98 2140 1545 2229
Fragments arranged by size:
3115 2700 2229 2140 1545 1219 900 667
527 259 145 98 97 33 14
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 15688
PciI A'CATG_T
Cuts at: 5832 7926 14587 5832
Size: 2094 6661 6933
Fragments arranged by size:
6933 6661 2094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4681 6268 6858 8374 10974 12433 12746 1108
Size: 3573 1587 590 1516 2600 1459 313 4050
Fragments arranged by size:
4050 3573 2600 1587 1516 1459 590 313
PfoI T'CCnGG_A
Cuts at: 8731 12471 12739 8731
Size: 3740 268 11680
Fragments arranged by size:
11680 3740 268
PmeI GTTT'AAAC
Cuts at: 12992 12992
Size: 15688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6915 6947 9039 9071 10825 10857 11769 11801 13757
Size: 32 2092 32 1754 32 912 32 1956
Cuts at: 13757 13789 15270 15302 6915
Size: 32 1481 32 7301
Fragments arranged by size:
7301 2092 1956 1754 1481 912 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3124 3266 3124
Size: 142 15546
PshAI GACnn'nnGTC
Cuts at: 1613 9780 12084 1613
Size: 8167 2304 5217
Fragments arranged by size:
8167 5217 2304
PsiI TTA'TAA
Cuts at: 5552 13258 5552
Size: 7706 7982
PspOMI G'GGCC_C
Cuts at: 6443 6443
Size: 15688
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3282 3314 4039 4071 7830 7862 3282
Size: 32 725 32 3759 32 11108
Fragments arranged by size:
11108 3759 725 32 32 32
PstI C_TGCA'G
Cuts at: 830 2847 4338 4906 4959 5140 8101 8971 9531
Size: 2017 1491 568 53 181 2961 870 560
Cuts at: 9531 9819 9888 9954 10137 10433 10707 11577 11915
Size: 288 69 66 183 296 274 870 338
Cuts at: 11915 11956 12625 13040 830
Size: 41 669 415 3478
Fragments arranged by size:
3478 2961 2017 1491 870 870 669 568
560 415 338 296 288 274 183 181
69 66 53 41
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 15688
PvuII CAG'CTG
Cuts at: 2195 3368 4220 6064 6361 6631 6876 8205 9859
Size: 1173 852 1844 297 270 245 1329 1654
Cuts at: 9859 10381 10871 11264 11464 11587 12716 12961 2195
Size: 522 490 393 200 123 1129 245 4922
Fragments arranged by size:
4922 1844 1654 1329 1173 1129 852 522
490 393 297 270 245 245 200 123
SacI G_AGCT'C
Cuts at: 4813 6652 7899 8904 10017 10053 4813
Size: 1839 1247 1005 1113 36 10448
Fragments arranged by size:
10448 1839 1247 1113 1005 36
SacII CC_GC'GG
Cuts at: 2072 2072
Size: 15688
SalI G'TCGA_C
Cuts at: 1936 13030 1936
Size: 11094 4594
SapI GCTCTTCn'nnn_
Cuts at: 8334 8441 10005 8334
Size: 107 1564 14017
Fragments arranged by size:
14017 1564 107
SbfI CC_TGCA'GG
Cuts at: 13040 13040
Size: 15688
ScaI AGT'ACT
Cuts at: 3223 5972 11011 13936 15648 3223
Size: 2749 5039 2925 1712 3263
Fragments arranged by size:
5039 3263 2925 2749 1712
SexAI A'CCwGG_T
Cuts at: 3336 10482 3336
Size: 7146 8542
SfcI C'TryA_G
Cuts at: 826 1045 2266 2790 2843 3633 4334 4666 4902
Size: 219 1221 524 53 790 701 332 236
Cuts at: 4902 4955 5136 6768 8097 8967 9527 9629 9815
Size: 53 181 1632 1329 870 560 102 186
Cuts at: 9815 9884 9950 10054 10133 10429 10703 11081 11573
Size: 69 66 104 79 296 274 378 492
Cuts at: 11573 11911 11952 12621 13036 14171 14852 15043 826
Size: 338 41 669 415 1135 681 191 1471
Fragments arranged by size:
1632 1471 1329 1221 1135 870 790 701
681 669 560 524 492 415 378 338
332 296 274 236 219 191 186 181
104 102 79 69 66 53 53 41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 15688
SmaI CCC'GGG
Cuts at: 1872 2022 2477 13015 1872
Size: 150 455 10538 4545
Fragments arranged by size:
10538 4545 455 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4157 4812 7551 10735 10911 11013
Size: 197 926 2214 655 2739 3184 176 102
Cuts at: 11013 11361 11786 13792 14693 14955 820
Size: 348 425 2006 901 262 1553
Fragments arranged by size:
3184 2739 2214 2006 1553 926 901 655
425 348 262 197 176 102
SnaBI TAC'GTA
Cuts at: 493 13479 493
Size: 12986 2702
SpeI A'CTAG_T
Cuts at: 152 2557 4420 152
Size: 2405 1863 11420
Fragments arranged by size:
11420 2405 1863
SphI G_CATG'C
Cuts at: 1132 4344 7403 13046 1132
Size: 3212 3059 5643 3774
Fragments arranged by size:
5643 3774 3212 3059
SspI AAT'ATT
Cuts at: 5 52 2135 3676 3708 4152 7224 7669 13612
Size: 47 2083 1541 32 444 3072 445 5943
Cuts at: 13612 5
Size: 2081
Fragments arranged by size:
5943 3072 2083 2081 1541 445 444 47
32
StuI AGG'CCT
Cuts at: 1517 3767 4293 5366 9251 10730 1517
Size: 2250 526 1073 3885 1479 6475
Fragments arranged by size:
6475 3885 2250 1479 1073 526
StyI C'CwwG_G
Cuts at: 513 1065 4921 5031 6179 6418 7151 7310 7457
Size: 552 3856 110 1148 239 733 159 147
Cuts at: 7457 7833 8294 9001 9778 10515 13127 513
Size: 376 461 707 777 737 2612 3074
Fragments arranged by size:
3856 3074 2612 1148 777 737 733 707
552 461 376 239 159 147 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2508 3015 7462 9143 9638 10873 11333
Size: 1255 928 507 4447 1681 495 1235 460
Cuts at: 11333 12362 12973 13715 13732 13885 14070 14515 325
Size: 1029 611 742 17 153 185 445 1498
Fragments arranged by size:
4447 1681 1498 1255 1235 1029 928 742
611 507 495 460 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2590 3221 3303
Size: 274 80 33 51 1443 613 631 82
Cuts at: 3303 5970 6832 7233 11009 11179 11824 13934 15646
Size: 2667 862 401 3776 170 645 2110 1712
Cuts at: 15646 96
Size: 138
Fragments arranged by size:
3776 2667 2110 1712 1443 862 645 631
613 401 274 170 138 82 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2050 2082 4701 4733 5375 5407 6745 6777 7017
Size: 32 2619 32 642 32 1338 32 240
Cuts at: 7017 7049 7633 7665 8077 8109 8550 8582 15648
Size: 32 584 32 412 32 441 32 7066
Cuts at: 15648 15680 2050
Size: 32 2058
Fragments arranged by size:
7066 2619 2058 1338 642 584 441 412
240 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3434 8357 12920 1316
Size: 2118 4923 4563 4084
Fragments arranged by size:
4923 4563 4084 2118
XbaI T'CTAG_A
Cuts at: 9398 9559 12943 13024 9398
Size: 161 3384 81 12062
Fragments arranged by size:
12062 3384 161 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3140 5227 8627 10835 11812 3140
Size: 2087 3400 2208 977 7016
Fragments arranged by size:
7016 3400 2208 2087 977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 15688
XmaI C'CCGG_G
Cuts at: 1870 2020 2475 13013 1870
Size: 150 455 10538 4545
Fragments arranged by size:
10538 4545 455 150
XmnI GAAnn'nnTTC
Cuts at: 2529 2699 4121 6930 9054 10611 10722 10840 12213
Size: 170 1422 2809 2124 1557 111 118 1373
Cuts at: 12213 12339 13817 2529
Size: 126 1478 4400
Fragments arranged by size:
4400 2809 2124 1557 1478 1422 1373 170
126 118 111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 13496 276
Size: 53 83 186 936 11962 2468
Fragments arranged by size:
11962 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI BbeI BsiWI FseI FspAI KasI NarI
NruI PacI PmlI PspXI RsrII SanDI SfiI SfoI
SrfI SwaI