Restriction map for
pFN21AB9893
(Circular) (Six-base) MAPSORT of: pFN21AB9893.seq Check: 5927 from: 1 to: 8581
pFN21AB9893 8581 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6391 278
Size: 53 83 186 936 4855 2468
Fragments arranged by size:
4855 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 2805 4678 4894 5924 1937
Size: 514 354 1873 216 1030 4594
Fragments arranged by size:
4594 1873 1030 514 354 216
Acc65I G'GTAC_C
Cuts at: 4216 5902 4216
Size: 1686 6895
AclI AA'CG_TT
Cuts at: 1563 4816 6708 7081 1563
Size: 3253 1892 373 3063
Fragments arranged by size:
3253 3063 1892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3229 3374 3421 3519 3565 4178 4242 5642
Size: 1597 145 47 98 46 613 64 1400
Cuts at: 5642 5699 5846 6644 8028 8296 1632
Size: 57 147 798 1384 268 1917
Fragments arranged by size:
1917 1597 1400 1384 798 613 268 147
145 98 64 57 47 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8384
AflIII A'CryG_T
Cuts at: 1203 2262 7480 8457 1203
Size: 1059 5218 977 1327
Fragments arranged by size:
5218 1327 1059 977
AgeI A'CCGG_T
Cuts at: 7412 7412
Size: 8581
AhdI GACnn_n'nnGTC
Cuts at: 2027 2678 4275 7310 2027
Size: 651 1597 3035 3298
Fragments arranged by size:
3298 3035 1597 651
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5858 1184
Size: 4674 3907
AlwNI CAG_nnn'CTG
Cuts at: 2688 3352 5837 6324 7896 2688
Size: 664 2485 487 1572 3373
Fragments arranged by size:
3373 2485 1572 664 487
ApaLI G'TGCA_C
Cuts at: 6638 7794 6638
Size: 1156 7425
ApoI r'AATT_y
Cuts at: 1493 2305 2462 2755 3068 3097 3629 4691 4773
Size: 812 157 293 313 29 532 1062 82
Cuts at: 4773 4901 5890 6116 6268 8377 1493
Size: 128 989 226 152 2109 1697
Fragments arranged by size:
2109 1697 1062 989 812 532 313 293
226 157 152 128 82 29
AseI AT'TA_AT
Cuts at: 160 1032 7135 160
Size: 872 6103 1606
Fragments arranged by size:
6103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8581
AvaI C'yCGr_G
Cuts at: 1870 1943 4938 5906 1870
Size: 73 2995 968 4545
Fragments arranged by size:
4545 2995 968 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2123 2156 2123
Size: 33 8548
BamHI G'GATC_C
Cuts at: 5775 5911 6278 5775
Size: 136 367 8078
Fragments arranged by size:
8078 367 136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4216 5282 5302 5902 7357
Size: 325 217 627 2429 1066 20 600 1455
Cuts at: 7357 618
Size: 1842
Fragments arranged by size:
2429 1842 1455 1066 627 600 325 217
20
BanII G_rGCy'C
Cuts at: 1391 2117 3987 4373 5206 5378 5900 1391
Size: 726 1870 386 833 172 522 4072
Fragments arranged by size:
4072 1870 833 726 522 386 172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7610
BbvCI CC'TCA_GC
Cuts at: 4824 5061 4824
Size: 237 8344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6772 6806 1903
Size: 34 4835 34 3678
Fragments arranged by size:
4835 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6473 7683 6473
Size: 1210 7371
BclI T'GATC_A
Cuts at: 1548 4314 1548
Size: 2766 5815
BfrBI ATG'CAT
Cuts at: 5528 5528
Size: 8581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7192 136
Size: 107 122 71 631 605 5520 1525
Fragments arranged by size:
5520 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8576 8576
Size: 8581
BlpI GC'TnA_GC
Cuts at: 5998 5998
Size: 8581
Bme1580I G_kGCm'C
Cuts at: 1790 5196 6642 7798 1790
Size: 3406 1446 1156 2573
Fragments arranged by size:
3406 2573 1446 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3670 4098 4986
Size: 416 53 83 399 24 2253 428 888
Cuts at: 4986 6312 7270 442
Size: 1326 958 1753
Fragments arranged by size:
2253 1753 1326 958 888 428 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4598 1056
Size: 3542 5039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3667 3667
Size: 8581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2321 2730 2849 3990 4691 7241 1239
Size: 974 108 409 119 1141 701 2550 2579
Fragments arranged by size:
2579 2550 1141 974 701 409 119 108
Bpu10I CC'TnA_GC
Cuts at: 2719 4180 4488 4824 5061 2719
Size: 1461 308 336 237 6239
Fragments arranged by size:
6239 1461 336 308 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3299 4081 6706 7571 7869 3299
Size: 782 2625 865 298 4011
Fragments arranged by size:
4011 2625 865 782 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4935 5484 7244 882
Size: 617 3436 549 1760 2219
Fragments arranged by size:
3436 2219 1760 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 2263 6372 493
Size: 711 1059 4109 2702
Fragments arranged by size:
4109 2702 1059 711
BsaBI GATnn'nnATC
Cuts at: 3834 8575 3834
Size: 4741 3840
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6388 6770 7405 275
Size: 53 83 186 936 4855 382 635 1451
Fragments arranged by size:
4855 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4347 5865 7014 7412 7686 7833 8244 4347
Size: 1518 1149 398 274 147 411 4684
Fragments arranged by size:
4684 1518 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2433 2463 2763 2793 4218
Size: 30 165 30 1030 30 300 30 1425
Cuts at: 4218 4248 5175 5205 1178
Size: 30 927 30 4554
Fragments arranged by size:
4554 1425 1030 927 300 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3692 5078 5397 5471 1186
Size: 2506 1386 319 74 4296
Fragments arranged by size:
4296 2506 1386 319 74
BseYI C'CCAG_C
Cuts at: 2069 2595 4354 7784 8280 2069
Size: 526 1759 3430 496 2370
Fragments arranged by size:
3430 2370 1759 526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 2664 3850 4822 1163
Size: 573 376 552 1186 972 4922
Fragments arranged by size:
4922 1186 972 573 552 376
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6031 6792 7820 1540
Size: 275 186 4030 761 1028 2301
Fragments arranged by size:
4030 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2117 2214 2835 3746 4437 5711 6642 6727 7798
Size: 97 621 911 691 1274 931 85 1071
Cuts at: 7798 2117
Size: 2900
Fragments arranged by size:
2900 1274 1071 931 911 691 621 97
85
BsmI GAATG_Cn'
Cuts at: 2864 6092 6185 2864
Size: 3228 93 5260
Fragments arranged by size:
5260 3228 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2214 2835 3746 3987 4373 4437
Size: 399 327 97 621 911 241 386 64
Cuts at: 4437 5196 5206 5378 5711 5900 6642 6727 7798
Size: 759 10 172 333 189 742 85 1071
Cuts at: 7798 1391
Size: 2174
Fragments arranged by size:
2174 1071 911 759 742 621 399 386
333 327 241 189 172 97 85 64
10
BspEI T'CCGG_A
Cuts at: 8244 8244
Size: 8581
BspHI T'CATG_A
Cuts at: 3895 6470 8200 3895
Size: 2575 1730 4276
Fragments arranged by size:
4276 2575 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 5739 5936 844
Size: 1457 3438 197 3489
Fragments arranged by size:
3489 3438 1457 197
BsrBI CCG'CTC
Cuts at: 1394 1823 6468 1394
Size: 429 4645 3507
Fragments arranged by size:
4645 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 7076 7250 65
Size: 1183 1366 4462 174 1396
Fragments arranged by size:
4462 1396 1366 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 7225 7412 1700
Size: 110 201 5214 187 2869
Fragments arranged by size:
5214 2869 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 3734 96
Size: 3638 4943
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8581
BssSI C'ACGA_G
Cuts at: 2045 4430 5379 6641 7653 2045
Size: 2385 949 1262 1012 2973
Fragments arranged by size:
2973 2385 1262 1012 949
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5243 794
Size: 4449 4132
BstBI TT'CG_AA
Cuts at: 7385 7385
Size: 8581
BstEII G'GTnAC_C
Cuts at: 1184 3265 1184
Size: 2081 6500
BstXI CCAn_nnnn'nTGG
Cuts at: 3661 4384 4606 4659 5602 3661
Size: 723 222 53 943 6640
Fragments arranged by size:
6640 943 723 222 53
BstYI r'GATC_y
Cuts at: 1972 3594 5775 5911 6278 6663 6680 8132 8218
Size: 1622 2181 136 367 385 17 1452 86
Cuts at: 8218 8230 8576 1972
Size: 12 346 1977
Fragments arranged by size:
2181 1977 1622 1452 385 367 346 136
86 17 12
BstZ17I GTA'TAC
Cuts at: 4679 4679
Size: 8581
Bsu36I CC'TnA_GG
Cuts at: 2727 5079 2727
Size: 2352 6229
BtgI C'CryG_G
Cuts at: 513 1065 2513 3712 3914 4405 513
Size: 552 1448 1199 202 491 4689
Fragments arranged by size:
4689 1448 1199 552 491 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7842
Fragments arranged by size:
7842 683 56
BtsI GCAGTG_nn'
Cuts at: 6097 6891 6911 6097
Size: 794 20 7767
Fragments arranged by size:
7767 794 20
ClaI AT'CG_AT
Cuts at: 1554 2344 1554
Size: 790 7791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2749 2784 3753 3788 5341 5376 529
Size: 35 2185 35 969 35 1553 35 3734
Fragments arranged by size:
3734 2185 1553 969 35 35 35 35
DraI TTT'AAA
Cuts at: 2367 3754 5885 2367
Size: 1387 2131 5063
Fragments arranged by size:
5063 2131 1387
DraIII CAC_nnn'GTG
Cuts at: 5243 5243
Size: 8581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2536 7588 809
Size: 507 1220 5052 1802
Fragments arranged by size:
5052 1802 1220 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2587 3224 6028
Size: 54 1425 50 275 18 757 637 2804
Cuts at: 6028 6917 8546 8
Size: 889 1629 43
Fragments arranged by size:
2804 1629 1425 889 757 637 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6028 1537
Size: 275 4216 4090
Fragments arranged by size:
4216 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3274 3440 3992 4031 4154 5028 6511 1353
Size: 1921 166 552 39 123 874 1483 3423
Fragments arranged by size:
3423 1921 1483 874 552 166 123 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5788 7168 7542 7688 387
Size: 1064 138 4199 1380 374 146 1280
Fragments arranged by size:
4199 1380 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2115 2115
Size: 8581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2321 2730 2849 3229 3374 3421
Size: 393 581 108 409 119 380 145 47
Cuts at: 3421 3519 3565 3990 4178 4242 4691 5642 5699
Size: 98 46 425 188 64 449 951 57
Cuts at: 5699 5846 6644 7241 8028 8296 1239
Size: 147 798 597 787 268 1524
Fragments arranged by size:
1524 951 798 787 597 581 449 425
409 393 380 268 188 147 145 119
108 98 64 57 47 46
EcoO109I rG'GnC_Cy
Cuts at: 2221 2600 2723 3130 3545 3722 2221
Size: 379 123 407 415 177 7080
Fragments arranged by size:
7080 415 407 379 177 123
EcoRI G'AATT_C
Cuts at: 4773 5890 6268 4773
Size: 1117 378 7086
Fragments arranged by size:
7086 1117 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4148 4148
Size: 8581
FspI TGC'GCA
Cuts at: 2153 7087 2153
Size: 4934 3647
HaeII r_GCGC'y
Cuts at: 1678 7728 1678
Size: 6050 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2066 2098 2664
Size: 32 135 32 441 32 21 32 566
Cuts at: 2664 2696 3022 3054 3579 3611 4261 4293 7222
Size: 32 326 32 525 32 650 32 2929
Cuts at: 7222 7254 7296 7328 1373
Size: 32 42 32 2626
Fragments arranged by size:
2929 2626 650 566 525 441 326 135
42 32 32 32 32 32 32 32
32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2541 5925 6171 669
Size: 988 281 603 3384 246 3079
Fragments arranged by size:
3384 3079 988 603 281 246
HindIII A'AGCT_T
Cuts at: 748 3000 4985 748
Size: 2252 1985 4344
Fragments arranged by size:
4344 2252 1985
HpaI GTT'AAC
Cuts at: 6171 6171
Size: 8581
KpnI G_GTAC'C
Cuts at: 4220 5906 4220
Size: 1686 6895
MfeI C'AATT_G
Cuts at: 3144 6180 3144
Size: 3036 5545
MluI A'CGCG_T
Cuts at: 8457 8457
Size: 8581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2547 2837 4398 4802 4853 7695 7879 1305
Size: 1242 290 1561 404 51 2842 184 2007
Fragments arranged by size:
2842 2007 1561 1242 404 290 184 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3226 10
Size: 54 1425 343 1394 5365
Fragments arranged by size:
5365 1425 1394 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3659 4704 6539 6898 7057 518
Size: 648 2493 1045 1835 359 159 2042
Fragments arranged by size:
2493 2042 1835 1045 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3968 4384 5997 6674 7822 8067 1606
Size: 2362 416 1613 677 1148 245 2120
Fragments arranged by size:
2362 2120 1613 1148 677 416 245
NaeI GCC'GGC
Cuts at: 2013 2013
Size: 8581
NcoI C'CATG_G
Cuts at: 513 1065 2513 3712 3914 4405 513
Size: 552 1448 1199 202 491 4689
Fragments arranged by size:
4689 1448 1199 552 491 202
NdeI CA'TA_TG
Cuts at: 387 2857 387
Size: 2470 6111
NgoMIV G'CCGG_C
Cuts at: 2011 2011
Size: 8581
NheI G'CTAG_C
Cuts at: 1052 4594 1052
Size: 3542 5039
NotI GC'GGCC_GC
Cuts at: 6028 6028
Size: 8581
NsiI A_TGCA'T
Cuts at: 5530 5530
Size: 8581
NspI r_CATG'y
Cuts at: 1132 1229 2778 3711 3947 5939 7484 1132
Size: 97 1549 933 236 1992 1545 2229
Fragments arranged by size:
2229 1992 1549 1545 933 236 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8581
PciI A'CATG_T
Cuts at: 7480 7480
Size: 8581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2490 3423 1108
Size: 1382 933 6266
Fragments arranged by size:
6266 1382 933
PfoI T'CCnGG_A
Cuts at: 3465 3465
Size: 8581
PmeI GTTT'AAAC
Cuts at: 5885 5885
Size: 8581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6650 6682 8163 8195 6650
Size: 32 1481 32 7036
Fragments arranged by size:
7036 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2221 2600 2723 3130 3545 3722 2221
Size: 379 123 407 415 177 7080
Fragments arranged by size:
7080 415 407 379 177 123
PshAI GACnn'nnGTC
Cuts at: 1613 3561 1613
Size: 1948 6633
PsiI TTA'TAA
Cuts at: 4203 5760 6151 4203
Size: 1557 391 6633
Fragments arranged by size:
6633 1557 391
PstI C_TGCA'G
Cuts at: 830 5736 5933 830
Size: 4906 197 3478
Fragments arranged by size:
4906 3478 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8581
PvuII CAG'CTG
Cuts at: 3968 4384 3968
Size: 416 8165
SacI G_AGCT'C
Cuts at: 2117 2117
Size: 8581
SalI G'TCGA_C
Cuts at: 1936 5923 1936
Size: 3987 4594
SanDI GG'GwC_CC
Cuts at: 2600 3130 2600
Size: 530 8051
SapI GCTCTTCn'nnn_
Cuts at: 3992 4031 3992
Size: 39 8542
SbfI CC_TGCA'GG
Cuts at: 5736 5933 5736
Size: 197 8384
ScaI AGT'ACT
Cuts at: 2159 3285 5069 6829 8541 2159
Size: 1126 1784 1760 1712 2199
Fragments arranged by size:
2199 1784 1760 1712 1126
SexAI A'CCwGG_T
Cuts at: 3261 3261
Size: 8581
SfcI C'TryA_G
Cuts at: 826 1045 2371 3289 3338 5197 5732 5929 7064
Size: 219 1326 918 49 1859 535 197 1135
Cuts at: 7064 7745 7936 826
Size: 681 191 1471
Fragments arranged by size:
1859 1471 1326 1135 918 681 535 219
197 191 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8581
SmaI CCC'GGG
Cuts at: 1872 5908 1872
Size: 4036 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3314 4060 4938 6685 7586 7848
Size: 197 926 1371 746 878 1747 901 262
Cuts at: 7848 820
Size: 1553
Fragments arranged by size:
1747 1553 1371 926 901 878 746 262
197
SnaBI TAC'GTA
Cuts at: 493 6372 493
Size: 5879 2702
SpeI A'CTAG_T
Cuts at: 152 3890 152
Size: 3738 4843
SphI G_CATG'C
Cuts at: 1132 5939 1132
Size: 4807 3774
SspI AAT'ATT
Cuts at: 5 52 2361 5753 6505 5
Size: 47 2309 3392 752 2081
Fragments arranged by size:
3392 2309 2081 752 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8581
StyI C'CwwG_G
Cuts at: 513 1065 2352 2513 3712 3871 3914 4174 4281
Size: 552 1287 161 1199 159 43 260 107
Cuts at: 4281 4405 5544 5601 6020 513
Size: 124 1139 57 419 3074
Fragments arranged by size:
3074 1287 1199 1139 552 419 260 161
159 124 107 57 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 3864 3884 4132 4346 4712 5345
Size: 1255 443 1841 20 248 214 366 633
Cuts at: 5345 5557 5736 6608 6625 6778 6963 7408 325
Size: 212 179 872 17 153 185 445 1498
Fragments arranged by size:
1841 1498 1255 872 633 445 443 366
248 214 212 185 179 153 20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2582 2850
Size: 274 80 33 51 1443 180 425 268
Cuts at: 2850 3283 3491 3734 4626 5067 5739 6827 8539
Size: 433 208 243 892 441 672 1088 1712
Cuts at: 8539 96
Size: 138
Fragments arranged by size:
1712 1443 1088 892 672 441 433 425
274 268 243 208 180 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 4218 4250 4329 4361 5175 5207 5295
Size: 32 1560 32 79 32 814 32 88
Cuts at: 5295 5327 5513 5545 8541 8573 2626
Size: 32 186 32 2996 32 2634
Fragments arranged by size:
2996 2634 1560 814 186 88 79 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3720 1316
Size: 2404 6177
XbaI T'CTAG_A
Cuts at: 5917 5917
Size: 8581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2824 4288 2824
Size: 1464 7117
XhoI C'TCGA_G
Cuts at: 1943 4938 1943
Size: 2995 5586
XmaI C'CCGG_G
Cuts at: 1870 5906 1870
Size: 4036 4545
XmnI GAAnn'nnTTC
Cuts at: 2462 6710 2462
Size: 4248 4333
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6389 276
Size: 53 83 186 936 4855 2468
Fragments arranged by size:
4855 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BsmBI EcoNI EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI