Restriction map for
pFN21AB9889
(Circular) (Six-base) MAPSORT of: pFN21AB9889.seq Check: 2751 from: 1 to: 7834
pFN21AB9889 7834 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5644 278
Size: 53 83 186 936 4108 2468
Fragments arranged by size:
4108 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4185 5177 1937
Size: 2248 992 4594
Fragments arranged by size:
4594 2248 992
Acc65I G'GTAC_C
Cuts at: 5155 5155
Size: 7834
AclI AA'CG_TT
Cuts at: 1563 4279 5961 6334 1563
Size: 2716 1682 373 3063
Fragments arranged by size:
3063 2716 1682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 2666 3602 4102 4694 5897 7281 7549
Size: 519 515 936 500 592 1203 1384 268
Cuts at: 7549 1632
Size: 1917
Fragments arranged by size:
1917 1384 1203 936 592 519 515 500
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7637
AflIII A'CryG_T
Cuts at: 1203 6733 7710 1203
Size: 5530 977 1327
Fragments arranged by size:
5530 1327 977
AgeI A'CCGG_T
Cuts at: 6665 6665
Size: 7834
AhdI GACnn_n'nnGTC
Cuts at: 6563 6563
Size: 7834
AleI CACnn'nnGTG
Cuts at: 1166 3791 1166
Size: 2625 5209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3106 1184
Size: 1922 5912
AlwNI CAG_nnn'CTG
Cuts at: 5577 7149 5577
Size: 1572 6262
ApaLI G'TGCA_C
Cuts at: 3164 5891 7047 3164
Size: 2727 1156 3951
Fragments arranged by size:
3951 2727 1156
ApoI r'AATT_y
Cuts at: 1493 2055 2075 2090 2273 2350 3058 5129 5143
Size: 562 20 15 183 77 708 2071 14
Cuts at: 5143 5369 5521 7630 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 2071 1697 708 562 226 183 152
77 20 15 14
AseI AT'TA_AT
Cuts at: 160 1032 3171 4073 6388 160
Size: 872 2139 902 2315 1606
Fragments arranged by size:
2315 2139 1606 902 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7834
AvaI C'yCGr_G
Cuts at: 1870 1943 2593 4166 5159 1870
Size: 73 650 1573 993 4545
Fragments arranged by size:
4545 1573 993 650 73
AvrII C'CTAG_G
Cuts at: 2416 2416
Size: 7834
BamHI G'GATC_C
Cuts at: 5164 5531 5164
Size: 367 7467
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2616 4124 5155 6610 618
Size: 325 217 627 829 1508 1031 1455 1842
Fragments arranged by size:
1842 1508 1455 1031 829 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2764 3223 3246 3392 4770 5153 1391
Size: 1373 459 23 146 1378 383 4072
Fragments arranged by size:
4072 1378 1373 459 383 146 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2672 3585 3735 4231 928
Size: 971 773 913 150 496 4531
Fragments arranged by size:
4531 971 913 773 496 150
BbvCI CC'TCA_GC
Cuts at: 3236 3440 3236
Size: 204 7630
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2316 2350 2988 3022 3503 3537 4683
Size: 34 379 34 638 34 481 34 1146
Cuts at: 4683 4717 6025 6059 1903
Size: 34 1308 34 3678
Fragments arranged by size:
3678 1308 1146 638 481 379 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5726 6936 5726
Size: 1210 6624
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7834
BfrBI ATG'CAT
Cuts at: 5036 5036
Size: 7834
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6445 136
Size: 107 122 71 631 605 4773 1525
Fragments arranged by size:
4773 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2886 4431 7829 2886
Size: 1545 3398 2891
Fragments arranged by size:
3398 2891 1545
BlpI GC'TnA_GC
Cuts at: 2699 4764 5251 2699
Size: 2065 487 5282
Fragments arranged by size:
5282 2065 487
Bme1580I G_kGCm'C
Cuts at: 1790 2575 3168 4127 4721 5895 7051 1790
Size: 785 593 959 594 1174 1156 2573
Fragments arranged by size:
2573 1174 1156 959 785 594 593
BmgBI CAC'GTC
Cuts at: 1318 3826 1318
Size: 2508 5326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3653 5565 6523
Size: 416 53 83 399 24 2236 1912 958
Cuts at: 6523 442
Size: 1753
Fragments arranged by size:
2236 1912 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7834
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2778 4112 2778
Size: 1334 6500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2175 2201 2563 3044 3386 4994 6494 1239
Size: 936 26 362 481 342 1608 1500 2579
Fragments arranged by size:
2579 1608 1500 936 481 362 342 26
Bpu10I CC'TnA_GC
Cuts at: 2763 3236 3440 5077 2763
Size: 473 204 1637 5520
Fragments arranged by size:
5520 1637 473 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 3118 3230 4100 5959 6824 7122 2651
Size: 467 112 870 1859 865 298 3363
Fragments arranged by size:
3363 1859 870 865 467 298 112
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 4354 6497 882
Size: 617 1413 1442 2143 2219
Fragments arranged by size:
2219 2143 1442 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 5625 493
Size: 711 4421 2702
Fragments arranged by size:
4421 2702 711
BsaBI GATnn'nnATC
Cuts at: 7828 7828
Size: 7834
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5641 6023 6658 275
Size: 53 83 186 936 4108 382 635 1451
Fragments arranged by size:
4108 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2481 4098 4524 4926 6267 6665 6939 7086 7497
Size: 1617 426 402 1341 398 274 147 411
Cuts at: 7497 2481
Size: 2818
Fragments arranged by size:
2818 1617 1341 426 411 402 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7609
Fragments arranged by size:
7609 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3113 4646 1186
Size: 1927 1533 4374
Fragments arranged by size:
4374 1927 1533
BseYI C'CCAG_C
Cuts at: 4084 7037 7533 4084
Size: 2953 496 4385
Fragments arranged by size:
4385 2953 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5284 6045 7073 1540
Size: 275 186 3283 761 1028 2301
Fragments arranged by size:
3283 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2373 2764 2862 3168 3223 3246 3348 4770 5895
Size: 391 98 306 55 23 102 1422 1125
Cuts at: 5895 5980 7051 2373
Size: 85 1071 3156
Fragments arranged by size:
3156 1422 1125 1071 391 306 102 98
85 55 23
BsmI GAATG_Cn'
Cuts at: 2952 3532 4092 4942 5029 5345 5438 2952
Size: 580 560 850 87 316 93 5348
Fragments arranged by size:
5348 850 580 560 316 93 87
BsmBI CGTCTCn'nnnn_
Cuts at: 3816 4004 3816
Size: 188 7646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2373 2575 2764 2862 3168 3223 3246
Size: 399 583 202 189 98 306 55 23
Cuts at: 3246 3348 3392 4127 4721 4770 5153 5895 5980
Size: 102 44 735 594 49 383 742 85
Cuts at: 5980 7051 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 735 594 583 399 383
306 202 189 102 98 85 55 49
44 23
BspEI T'CCGG_A
Cuts at: 2481 4098 4524 4926 7497 2481
Size: 1617 426 402 2571 2818
Fragments arranged by size:
2818 2571 1617 426 402
BspHI T'CATG_A
Cuts at: 2790 3891 4551 4584 5723 7453 2790
Size: 1101 660 33 1139 1730 3171
Fragments arranged by size:
3171 1730 1139 1101 660 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5189 844
Size: 4345 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5721 1394
Size: 429 3898 3507
Fragments arranged by size:
3898 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2466 3100 3669 4038 4417 6329 6503
Size: 1183 1218 634 569 369 379 1912 174
Cuts at: 6503 65
Size: 1396
Fragments arranged by size:
1912 1396 1218 1183 634 569 379 369
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3183 3231 4127 6478 6665 1700
Size: 110 1373 48 896 2351 187 2869
Fragments arranged by size:
2869 2351 1373 896 187 110 48
BsrGI T'GTAC_A
Cuts at: 96 3465 4992 96
Size: 3369 1527 2938
Fragments arranged by size:
3369 2938 1527
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7834
BssSI C'ACGA_G
Cuts at: 4007 4640 5894 6906 4007
Size: 633 1254 1012 4935
Fragments arranged by size:
4935 1254 1012 633
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7834
BstBI TT'CG_AA
Cuts at: 6638 6638
Size: 7834
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7834
BstXI CCAn_nnnn'nTGG
Cuts at: 4216 4216
Size: 7834
BstYI r'GATC_y
Cuts at: 1972 2111 2886 3749 4431 4663 5164 5531 5916
Size: 139 775 863 682 232 501 367 385
Cuts at: 5916 5933 7385 7471 7483 7829 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 863 775 682 501 385 367
346 232 139 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5067 5067
Size: 7834
BtgI C'CryG_G
Cuts at: 513 1065 2004 2392 2607 513
Size: 552 939 388 215 5740
Fragments arranged by size:
5740 939 552 388 215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7095
Fragments arranged by size:
7095 683 56
BtsI GCAGTG_nn'
Cuts at: 2575 2649 2850 4072 5350 6144 6164 2575
Size: 74 201 1222 1278 794 20 4245
Fragments arranged by size:
4245 1278 1222 794 201 74 20
ClaI AT'CG_AT
Cuts at: 1554 2487 1554
Size: 933 6901
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2010 2045 529
Size: 35 1446 35 6318
Fragments arranged by size:
6318 1446 35 35
DraI TTT'AAA
Cuts at: 5138 5138
Size: 7834
DraIII CAC_nnn'GTG
Cuts at: 2866 2866
Size: 7834
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2742 6841 809
Size: 507 1426 4099 1802
Fragments arranged by size:
4099 1802 1426 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2456 3417 5281
Size: 54 1425 50 275 18 626 961 1864
Cuts at: 5281 6170 7799 8
Size: 889 1629 43
Fragments arranged by size:
1864 1629 1425 961 889 626 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5281 1537
Size: 275 3469 4090
Fragments arranged by size:
4090 3469 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 2752 4055 5764 1353
Size: 1316 83 1303 1709 3423
Fragments arranged by size:
3423 1709 1316 1303 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6421 6795 6941 387
Size: 1064 138 4832 374 146 1280
Fragments arranged by size:
4832 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2762 3221 3244 4768 2762
Size: 459 23 1524 5828
Fragments arranged by size:
5828 1524 459 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2175 2201 2563 2666 3044 3386
Size: 393 519 24 26 362 103 378 342
Cuts at: 3386 3602 4102 4694 4994 5897 6494 7281 7549
Size: 216 500 592 300 903 597 787 268
Cuts at: 7549 1239
Size: 1524
Fragments arranged by size:
1524 903 787 597 592 519 500 393
378 362 342 300 268 216 103 26
24
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 2204
Size: 7834
EcoO109I rG'GnC_Cy
Cuts at: 5083 5083
Size: 7834
EcoRI G'AATT_C
Cuts at: 2350 3058 5143 5521 2350
Size: 708 2085 378 4663
Fragments arranged by size:
4663 2085 708 378
EcoRV GAT'ATC
Cuts at: 2384 3002 2384
Size: 618 7216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2339 2953 4132 2176
Size: 163 614 1179 5878
Fragments arranged by size:
5878 1179 614 163
FseI GG_CCGG'CC
Cuts at: 3187 3187
Size: 7834
FspI TGC'GCA
Cuts at: 6340 6340
Size: 7834
HaeII r_GCGC'y
Cuts at: 1678 4924 6981 1678
Size: 3246 2057 2531
Fragments arranged by size:
3246 2531 2057
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2373 2405 2492 2524 2662
Size: 32 135 32 801 32 87 32 138
Cuts at: 2662 2694 3281 3313 3611 3643 6475 6507 6549
Size: 32 587 32 298 32 2832 32 42
Cuts at: 6549 6581 1373
Size: 32 2626
Fragments arranged by size:
2832 2626 801 587 298 138 135 87
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3430 5178 5424 669
Size: 988 281 568 924 1748 246 3079
Fragments arranged by size:
3079 1748 988 924 568 281 246
HindIII A'AGCT_T
Cuts at: 748 5074 748
Size: 4326 3508
HpaI GTT'AAC
Cuts at: 5424 5424
Size: 7834
KpnI G_GTAC'C
Cuts at: 5159 5159
Size: 7834
MfeI C'AATT_G
Cuts at: 5433 5433
Size: 7834
MluI A'CGCG_T
Cuts at: 7710 7710
Size: 7834
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4323 6948 7132 1305
Size: 3018 2625 184 2007
Fragments arranged by size:
3018 2625 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3419 10
Size: 54 1425 343 1587 4425
Fragments arranged by size:
4425 1587 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3791 3843 3890 4556 5792 6151 6310
Size: 648 2625 52 47 666 1236 359 159
Cuts at: 6310 518
Size: 2042
Fragments arranged by size:
2625 2042 1236 666 648 359 159 52
47
MspA1I CmG'CkG
Cuts at: 1606 3407 3444 4418 5250 5927 7075 7320 1606
Size: 1801 37 974 832 677 1148 245 2120
Fragments arranged by size:
2120 1801 1148 974 832 677 245 37
NaeI GCC'GGC
Cuts at: 3185 3185
Size: 7834
NcoI C'CATG_G
Cuts at: 513 1065 2004 2392 2607 513
Size: 552 939 388 215 5740
Fragments arranged by size:
5740 939 552 388 215
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7834
NgoMIV G'CCGG_C
Cuts at: 3183 3183
Size: 7834
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7834
NotI GC'GGCC_GC
Cuts at: 5281 5281
Size: 7834
NsiI A_TGCA'T
Cuts at: 5038 5038
Size: 7834
NspI r_CATG'y
Cuts at: 1132 1229 2936 3518 5192 6737 1132
Size: 97 1707 582 1674 1545 2229
Fragments arranged by size:
2229 1707 1674 1545 582 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7834
PciI A'CATG_T
Cuts at: 6733 6733
Size: 7834
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2393 2613 1108
Size: 1285 220 6329
Fragments arranged by size:
6329 1285 220
PfoI T'CCnGG_A
Cuts at: 2199 2199
Size: 7834
PmeI GTTT'AAAC
Cuts at: 5138 5138
Size: 7834
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5903 5935 7416 7448 5903
Size: 32 1481 32 6289
Fragments arranged by size:
6289 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5083 5083
Size: 7834
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7834
PsiI TTA'TAA
Cuts at: 5404 5404
Size: 7834
PspXI vC'TCGA_Gb
Cuts at: 4166 4166
Size: 7834
PstI C_TGCA'G
Cuts at: 830 2813 3402 3449 5186 830
Size: 1983 589 47 1737 3478
Fragments arranged by size:
3478 1983 1737 589 47
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7834
PvuII CAG'CTG
Cuts at: 3407 3444 4418 3407
Size: 37 974 6823
Fragments arranged by size:
6823 974 37
SacI G_AGCT'C
Cuts at: 2764 3223 3246 4770 2764
Size: 459 23 1524 5828
Fragments arranged by size:
5828 1524 459 23
SalI G'TCGA_C
Cuts at: 1936 5176 1936
Size: 3240 4594
SanDI GG'GwC_CC
Cuts at: 5083 5083
Size: 7834
SapI GCTCTTCn'nnn_
Cuts at: 2752 4055 2752
Size: 1303 6531
SbfI CC_TGCA'GG
Cuts at: 5186 5186
Size: 7834
ScaI AGT'ACT
Cuts at: 6082 7794 6082
Size: 1712 6122
SfcI C'TryA_G
Cuts at: 826 1045 2809 3398 3445 3852 5182 6317 6998
Size: 219 1764 589 47 407 1330 1135 681
Cuts at: 6998 7189 826
Size: 191 1471
Fragments arranged by size:
1764 1471 1330 1135 681 589 407 219
191 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7834
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7834
SmaI CCC'GGG
Cuts at: 1872 5161 1872
Size: 3289 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 3133 3245 4079 4166 5938
Size: 197 926 723 467 112 834 87 1772
Cuts at: 5938 6839 7101 820
Size: 901 262 1553
Fragments arranged by size:
1772 1553 926 901 834 723 467 262
197 112 87
SnaBI TAC'GTA
Cuts at: 493 5625 493
Size: 5132 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7834
SphI G_CATG'C
Cuts at: 1132 3518 5192 1132
Size: 2386 1674 3774
Fragments arranged by size:
3774 2386 1674
SspI AAT'ATT
Cuts at: 5 52 5758 5
Size: 47 5706 2081
Fragments arranged by size:
5706 2081 47
StuI AGG'CCT
Cuts at: 1517 2421 4164 1517
Size: 904 1743 5187
Fragments arranged by size:
5187 1743 904
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2392 2416 2472 2607 5273
Size: 552 939 24 364 24 56 135 2666
Cuts at: 5273 513
Size: 3074
Fragments arranged by size:
3074 2666 939 552 364 135 56 24
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2079 5861 5878 6031 6216 6661
Size: 1255 458 41 3782 17 153 185 445
Cuts at: 6661 325
Size: 1498
Fragments arranged by size:
3782 1498 1255 458 445 185 153 41
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3465 4193 4737
Size: 274 80 33 51 1443 1488 728 544
Cuts at: 4737 4992 6080 7792 96
Size: 255 1088 1712 138
Fragments arranged by size:
1712 1488 1443 1088 728 544 274 255
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2615 2647 7794 7826 2615
Size: 32 5147 32 2623
Fragments arranged by size:
5147 2623 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7834
XbaI T'CTAG_A
Cuts at: 5170 5170
Size: 7834
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4403 4597 4403
Size: 194 7640
XhoI C'TCGA_G
Cuts at: 1943 4166 1943
Size: 2223 5611
XmaI C'CCGG_G
Cuts at: 1870 5159 1870
Size: 3289 4545
XmnI GAAnn'nnTTC
Cuts at: 2898 5963 2898
Size: 3065 4769
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5642 276
Size: 53 83 186 936 4108 2468
Fragments arranged by size:
4108 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BsiWI
BstZ17I FspAI KasI NarI NruI PacI PmlI PspOMI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI