Restriction map for
pFN21AB9887
(Circular) (Six-base) MAPSORT of: pFN21AB9887.seq Check: 6216 from: 1 to: 6964
pFN21AB9887 6964 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2038 2038
Size: 6964
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4774 278
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4307 1937
Size: 2370 4594
Acc65I G'GTAC_C
Cuts at: 4285 4285
Size: 6964
AclI AA'CG_TT
Cuts at: 1563 5091 5464 1563
Size: 3528 373 3063
Fragments arranged by size:
3528 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2297 5027 6411 6679 1632
Size: 665 2730 1384 268 1917
Fragments arranged by size:
2730 1917 1384 665 268
AfeI AGC'GCT
Cuts at: 2284 2297 2284
Size: 13 6951
AflII C'TTAA_G
Cuts at: 820 1017 2776 820
Size: 197 1759 5008
Fragments arranged by size:
5008 1759 197
AflIII A'CryG_T
Cuts at: 1203 5863 6840 1203
Size: 4660 977 1327
Fragments arranged by size:
4660 1327 977
AgeI A'CCGG_T
Cuts at: 5795 5795
Size: 6964
AhdI GACnn_n'nnGTC
Cuts at: 3079 5693 3079
Size: 2614 4350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6964
AlwNI CAG_nnn'CTG
Cuts at: 3829 3859 4707 6279 3829
Size: 30 848 1572 4514
Fragments arranged by size:
4514 1572 848 30
ApaI G_GGCC'C
Cuts at: 2555 2555
Size: 6964
ApaLI G'TGCA_C
Cuts at: 5021 6177 5021
Size: 1156 5808
ApoI r'AATT_y
Cuts at: 1493 3128 4273 4499 4651 6760 1493
Size: 1635 1145 226 152 2109 1697
Fragments arranged by size:
2109 1697 1635 1145 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5518 160
Size: 872 4486 1606
Fragments arranged by size:
4486 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
AvaI C'yCGr_G
Cuts at: 1870 1943 2434 2546 2570 3005 3550 4289 1870
Size: 73 491 112 24 435 545 739 4545
Fragments arranged by size:
4545 739 545 491 435 112 73 24
BamHI G'GATC_C
Cuts at: 4294 4661 4294
Size: 367 6597
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3364 3580 4168 4285 5740
Size: 325 217 627 1577 216 588 117 1455
Cuts at: 5740 618
Size: 1842
Fragments arranged by size:
1842 1577 1455 627 588 325 217 216
117
BanII G_rGCy'C
Cuts at: 1391 2369 2457 2555 2562 3092 3390 3557 4283
Size: 978 88 98 7 530 298 167 726
Cuts at: 4283 1391
Size: 4072
Fragments arranged by size:
4072 978 726 530 298 167 98 88
7
BbeI G_GCGC'C
Cuts at: 4172 4172
Size: 6964
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3370 928
Size: 971 1471 4522
Fragments arranged by size:
4522 1471 971
BbvCI CC'TCA_GC
Cuts at: 2928 3123 3635 2928
Size: 195 512 6257
Fragments arranged by size:
6257 512 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3346 3380 5155 5189 1903
Size: 34 1409 34 1775 34 3678
Fragments arranged by size:
3678 1775 1409 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4856 6066 4856
Size: 1210 5754
BclI T'GATC_A
Cuts at: 1548 2748 1548
Size: 1200 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5575 136
Size: 107 122 71 631 605 337 3566 1525
Fragments arranged by size:
3566 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6959 6959
Size: 6964
BlpI GC'TnA_GC
Cuts at: 4381 4381
Size: 6964
Bme1580I G_kGCm'C
Cuts at: 1790 2477 2555 2685 2722 3263 3369 3607 5025
Size: 687 78 130 37 541 106 238 1418
Cuts at: 5025 6181 1790
Size: 1156 2573
Fragments arranged by size:
2573 1418 1156 687 541 238 130 106
78 37
BmgBI CAC'GTC
Cuts at: 1318 3643 1318
Size: 2325 4639
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2952 3670 4695
Size: 416 53 83 399 24 1535 718 1025
Cuts at: 4695 5653 442
Size: 958 1753
Fragments arranged by size:
1753 1535 1025 958 718 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2388 2388
Size: 6964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2889 3541 3560 4045 5624 1239
Size: 1650 652 19 485 1579 2579
Fragments arranged by size:
2579 1650 1579 652 485 19
Bpu10I CC'TnA_GC
Cuts at: 2382 2556 2607 2928 3123 3635 4145 2382
Size: 174 51 321 195 512 510 5201
Fragments arranged by size:
5201 512 510 321 195 174 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3027 5089 5954 6252 3027
Size: 2062 865 298 3739
Fragments arranged by size:
3739 2062 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2419 2445 5627 882
Size: 617 920 26 3182 2219
Fragments arranged by size:
3182 2219 920 617 26
BsaAI yAC'GTr
Cuts at: 493 1204 2188 3893 4755 493
Size: 711 984 1705 862 2702
Fragments arranged by size:
2702 1705 984 862 711
BsaBI GATnn'nnATC
Cuts at: 6958 6958
Size: 6964
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3630 3843 3953 4169
Size: 53 83 186 936 2097 213 110 216
Cuts at: 4169 4771 5153 5788 275
Size: 602 382 635 1451
Fragments arranged by size:
2097 1451 936 635 602 382 216 213
186 110 83 53
BsaWI w'CCGG_w
Cuts at: 2538 3625 3838 5397 5795 6069 6216 6627 2538
Size: 1087 213 1559 398 274 147 411 2875
Fragments arranged by size:
2875 1559 1087 411 398 274 213 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3539 3569 4043 4073 1178
Size: 30 165 30 2136 30 474 30 4069
Fragments arranged by size:
4069 2136 474 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2912 3023 3122 1186
Size: 1105 621 111 99 5028
Fragments arranged by size:
5028 1105 621 111 99
BseYI C'CCAG_C
Cuts at: 2488 3368 6167 6663 2488
Size: 880 2799 496 2789
Fragments arranged by size:
2799 2789 880 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2012 3204 3873 1163
Size: 573 276 1192 669 4254
Fragments arranged by size:
4254 1192 669 573 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2503 2971 3034 3336 4414 5175
Size: 275 186 502 468 63 302 1078 761
Cuts at: 5175 6203 1540
Size: 1028 2301
Fragments arranged by size:
2301 1078 1028 761 502 468 302 275
186 63
BsiHKAI G_wGCw'C
Cuts at: 2238 2388 3092 3390 4164 5025 5110 6181 2238
Size: 150 704 298 774 861 85 1071 3021
Fragments arranged by size:
3021 1071 861 774 704 298 150 85
BsmI GAATG_Cn'
Cuts at: 3535 4475 4568 3535
Size: 940 93 5931
Fragments arranged by size:
5931 940 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2697 3987 2697
Size: 1290 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2369 2388 2457 2477 2555 2562
Size: 399 448 131 19 69 20 78 7
Cuts at: 2562 2685 2722 3092 3263 3369 3390 3557 3607
Size: 123 37 370 171 106 21 167 50
Cuts at: 3607 4164 4283 5025 5110 6181 1391
Size: 557 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 448 399 370 171
167 131 123 119 106 85 78 69
50 37 21 20 19 7
BspEI T'CCGG_A
Cuts at: 2538 3625 3838 6627 2538
Size: 1087 213 2789 2875
Fragments arranged by size:
2875 2789 1087 213
BspHI T'CATG_A
Cuts at: 4853 6583 4853
Size: 1730 5234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2038 3837 4319 844
Size: 1194 1799 482 3489
Fragments arranged by size:
3489 1799 1194 482
BsrBI CCG'CTC
Cuts at: 1394 1823 4851 1394
Size: 429 3028 3507
Fragments arranged by size:
3507 3028 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4162 5459 5633 65
Size: 1183 2914 1297 174 1396
Fragments arranged by size:
2914 1396 1297 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3335 3541 5608 5795 1700
Size: 110 1525 206 2067 187 2869
Fragments arranged by size:
2869 2067 1525 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6964
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6964
BssSI C'ACGA_G
Cuts at: 2237 3264 5024 6036 2237
Size: 1027 1760 1012 3165
Fragments arranged by size:
3165 1760 1027 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3829 794
Size: 3035 3929
BstBI TT'CG_AA
Cuts at: 5768 5768
Size: 6964
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6964
BstXI CCAn_nnnn'nTGG
Cuts at: 4207 4207
Size: 6964
BstYI r'GATC_y
Cuts at: 1972 4294 4661 5046 5063 6515 6601 6613 6959
Size: 2322 367 385 17 1452 86 12 346
Cuts at: 6959 1972
Size: 1977
Fragments arranged by size:
2322 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2274 2274
Size: 6964
BtgI C'CryG_G
Cuts at: 513 1065 2004 4038 4173 513
Size: 552 939 2034 135 3304
Fragments arranged by size:
3304 2034 939 552 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6225
Fragments arranged by size:
6225 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 3216 3605 4480 5274 5294 2040
Size: 1176 389 875 794 20 3710
Fragments arranged by size:
3710 1176 875 794 389 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3205 3240 529
Size: 35 2641 35 4253
Fragments arranged by size:
4253 2641 35 35
DraI TTT'AAA
Cuts at: 4268 4268
Size: 6964
DraIII CAC_nnn'GTG
Cuts at: 2035 2035
Size: 6964
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5971 809
Size: 507 4655 1802
Fragments arranged by size:
4655 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2500 2875
Size: 54 1425 50 275 18 228 442 375
Cuts at: 2875 2968 3333 3945 4176 4221 4411 5300 6929
Size: 93 365 612 231 45 190 889 1629
Cuts at: 6929 8
Size: 43
Fragments arranged by size:
1629 1425 889 612 442 375 365 275
231 228 190 93 54 50 45 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2500 2968 3333 4411 1537
Size: 275 688 468 365 1078 4090
Fragments arranged by size:
4090 1078 688 468 365 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 2581 2915 2945 3487 4894 1353
Size: 963 265 334 30 542 1407 3423
Fragments arranged by size:
3423 1407 963 542 334 265 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2098 2153 3337 5551 5925 6071
Size: 1064 138 509 55 1184 2214 374 146
Cuts at: 6071 387
Size: 1280
Fragments arranged by size:
2214 1280 1184 1064 509 374 146 138
55
EcoICRI GAG'CTC
Cuts at: 3090 3388 3090
Size: 298 6666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2889 3541 3560 4045 5027 5624
Size: 393 665 592 652 19 485 982 597
Cuts at: 5624 6411 6679 1239
Size: 787 268 1524
Fragments arranged by size:
1524 982 787 665 652 597 592 485
393 268 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3098 3401 3098
Size: 303 6661
EcoO109I rG'GnC_Cy
Cuts at: 2269 2551 2552 2769 2974 3394 3652 2269
Size: 282 1 217 205 420 258 5581
Fragments arranged by size:
5581 420 282 258 217 205 1
EcoRI G'AATT_C
Cuts at: 3128 4273 4651 3128
Size: 1145 378 5441
Fragments arranged by size:
5441 1145 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3038 3038
Size: 6964
FspI TGC'GCA
Cuts at: 5470 5470
Size: 6964
HaeII r_GCGC'y
Cuts at: 1678 2170 2286 2299 3342 4172 6111 1678
Size: 492 116 13 1043 830 1939 2531
Fragments arranged by size:
2531 1939 1043 830 492 116 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2720 2752 2944 2976 3065
Size: 32 135 32 1148 32 192 32 89
Cuts at: 3065 3097 3431 3463 3682 3714 5605 5637 5679
Size: 32 334 32 219 32 1891 32 42
Cuts at: 5679 5711 1373
Size: 32 2626
Fragments arranged by size:
2626 1891 1148 334 219 192 135 89
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4308 4554 669
Size: 988 281 2370 246 3079
Fragments arranged by size:
3079 2370 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6964
HpaI GTT'AAC
Cuts at: 4554 4554
Size: 6964
KasI G'GCGC_C
Cuts at: 4168 4168
Size: 6964
KpnI G_GTAC'C
Cuts at: 4289 4289
Size: 6964
MfeI C'AATT_G
Cuts at: 4563 4563
Size: 6964
MluI A'CGCG_T
Cuts at: 6840 6840
Size: 6964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2543 3051 6078 6262 1305
Size: 1238 508 3027 184 2007
Fragments arranged by size:
3027 2007 1238 508 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 3947 4223 10
Size: 54 1425 343 228 1887 276 2751
Fragments arranged by size:
2751 1887 1425 343 276 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4922 5281 5440 518
Size: 648 3756 359 159 2042
Fragments arranged by size:
3756 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2492 2564 2874 3703 3856 4040 4077 4175
Size: 886 72 310 829 153 184 37 98
Cuts at: 4175 4216 4380 5057 6205 6450 1606
Size: 41 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 886 829 677 310 245 184
164 153 98 72 41 37
NaeI GCC'GGC
Cuts at: 3337 3337
Size: 6964
NarI GG'CG_CC
Cuts at: 4169 4169
Size: 6964
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5473
Fragments arranged by size:
5473 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6964
NgoMIV G'CCGG_C
Cuts at: 3335 3335
Size: 6964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6964
NotI GC'GGCC_GC
Cuts at: 4411 4411
Size: 6964
NruI TCG'CGA
Cuts at: 2451 2451
Size: 6964
NspI r_CATG'y
Cuts at: 1132 1229 4322 5867 1132
Size: 97 3093 1545 2229
Fragments arranged by size:
3093 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2650 2722 1348
Size: 1302 72 5590
Fragments arranged by size:
5590 1302 72
PciI A'CATG_T
Cuts at: 5863 5863
Size: 6964
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 2984 1108
Size: 1080 796 5088
Fragments arranged by size:
5088 1080 796
PfoI T'CCnGG_A
Cuts at: 3303 3495 3774 3303
Size: 192 279 6493
Fragments arranged by size:
6493 279 192
PmeI GTTT'AAAC
Cuts at: 4268 4268
Size: 6964
PmlI CAC'GTG
Cuts at: 2188 3893 2188
Size: 1705 5259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5033 5065 6546 6578 5033
Size: 32 1481 32 5419
Fragments arranged by size:
5419 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2974 3394 3652 2269
Size: 705 420 258 5581
Fragments arranged by size:
5581 705 420 258
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6964
PsiI TTA'TAA
Cuts at: 4534 4534
Size: 6964
PspOMI G'GGCC_C
Cuts at: 2551 2551
Size: 6964
PstI C_TGCA'G
Cuts at: 830 2049 3330 3991 4316 830
Size: 1219 1281 661 325 3478
Fragments arranged by size:
3478 1281 1219 661 325
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6964
PvuII CAG'CTG
Cuts at: 2492 3703 2492
Size: 1211 5753
SacI G_AGCT'C
Cuts at: 3092 3390 3092
Size: 298 6666
SacII CC_GC'GG
Cuts at: 4041 4176 4041
Size: 135 6829
SalI G'TCGA_C
Cuts at: 1936 4306 1936
Size: 2370 4594
SanDI GG'GwC_CC
Cuts at: 2974 2974
Size: 6964
SapI GCTCTTCn'nnn_
Cuts at: 2316 2316
Size: 6964
SbfI CC_TGCA'GG
Cuts at: 4316 4316
Size: 6964
ScaI AGT'ACT
Cuts at: 5212 6924 5212
Size: 1712 5252
SfcI C'TryA_G
Cuts at: 826 1045 2045 3326 3987 4312 5447 6128 6319
Size: 219 1000 1281 661 325 1135 681 191
Cuts at: 6319 826
Size: 1471
Fragments arranged by size:
1471 1281 1135 1000 681 661 325 219
191
SfoI GGC'GCC
Cuts at: 4170 4170
Size: 6964
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6964
SmaI CCC'GGG
Cuts at: 1872 2572 4291 1872
Size: 700 1719 4545
Fragments arranged by size:
4545 1719 700
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2776 3042 5068 5969 6231 820
Size: 197 926 833 266 2026 901 262 1553
Fragments arranged by size:
2026 1553 926 901 833 266 262 197
SnaBI TAC'GTA
Cuts at: 493 4755 493
Size: 4262 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6964
SphI G_CATG'C
Cuts at: 1132 4322 1132
Size: 3190 3774
SspI AAT'ATT
Cuts at: 5 52 2093 4888 5
Size: 47 2041 2795 2081
Fragments arranged by size:
2795 2081 2041 47
StuI AGG'CCT
Cuts at: 1517 2330 2926 3407 3562 1517
Size: 813 596 481 155 4919
Fragments arranged by size:
4919 813 596 481 155
StyI C'CwwG_G
Cuts at: 513 1065 2004 2792 2892 3255 4224 4403 513
Size: 552 939 788 100 363 969 179 3074
Fragments arranged by size:
3074 969 939 788 552 363 179 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2259 4991 5008 5161 5346 5791 325
Size: 1255 679 2732 17 153 185 445 1498
Fragments arranged by size:
2732 1498 1255 679 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3546 5210 6922
Size: 274 80 33 51 1443 1569 1664 1712
Cuts at: 6922 96
Size: 138
Fragments arranged by size:
1712 1664 1569 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3477 3509 3510 3542 4043 4075 6924 6956 3477
Size: 32 1 32 501 32 2849 32 3485
Fragments arranged by size:
3485 2849 501 32 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6964
XbaI T'CTAG_A
Cuts at: 2962 4300 2962
Size: 1338 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6964
XmaI C'CCGG_G
Cuts at: 1870 2570 4289 1870
Size: 700 1719 4545
Fragments arranged by size:
4545 1719 700
XmnI GAAnn'nnTTC
Cuts at: 3675 5093 3675
Size: 1418 5546
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4772 276
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NsiI PacI PspXI PsrI RsrII SexAI
SfiI SrfI SwaI XcmI