Restriction map for
pFN21AB9886
(Circular) (Six-base) MAPSORT of: pFN21AB9886.seq Check: 6669 from: 1 to: 6880
pFN21AB9886 6880 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3514 3514
Size: 6880
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4690 278
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2193 4223 1937
Size: 256 2030 4594
Fragments arranged by size:
4594 2030 256
Acc65I G'GTAC_C
Cuts at: 4201 4201
Size: 6880
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 3048 3738 3920 4943 6327 6595 1632
Size: 569 847 690 182 1023 1384 268 1917
Fragments arranged by size:
1917 1384 1023 847 690 569 268 182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6683
AflIII A'CryG_T
Cuts at: 1203 2751 5779 6756 1203
Size: 1548 3028 977 1327
Fragments arranged by size:
3028 1548 1327 977
AgeI A'CCGG_T
Cuts at: 5711 5711
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 5609 5609
Size: 6880
AleI CACnn'nnGTG
Cuts at: 1166 3179 1166
Size: 2013 4867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2543 1184
Size: 1359 5521
AlwNI CAG_nnn'CTG
Cuts at: 2155 2179 3317 3446 3456 3560 3598 4623 6195
Size: 24 1138 129 10 104 38 1025 1572
Cuts at: 6195 2155
Size: 2840
Fragments arranged by size:
2840 1572 1138 1025 129 104 38 24
10
ApaI G_GGCC'C
Cuts at: 2854 2854
Size: 6880
ApaLI G'TGCA_C
Cuts at: 2612 3274 4937 6093 2612
Size: 662 1663 1156 3399
Fragments arranged by size:
3399 1663 1156 662
ApoI r'AATT_y
Cuts at: 1493 3422 4189 4415 4567 6676 1493
Size: 1929 767 226 152 2109 1697
Fragments arranged by size:
2109 1929 1697 767 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 2432 2774 2898 3407 4205 1870
Size: 73 489 342 124 509 798 4545
Fragments arranged by size:
4545 798 509 489 342 124 73
BamHI G'GATC_C
Cuts at: 3012 4003 4210 4577 3012
Size: 991 207 367 5315
Fragments arranged by size:
5315 991 367 207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2438 3353 4201 5656 618
Size: 325 217 627 651 915 848 1455 1842
Fragments arranged by size:
1842 1455 915 848 651 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2854 4199 1391
Size: 1463 1345 4072
Fragments arranged by size:
4072 1463 1345
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3036 3382 3980 928
Size: 971 1137 346 598 3828
Fragments arranged by size:
3828 1137 971 598 346
BbvCI CC'TCA_GC
Cuts at: 2661 2661
Size: 6880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2023 2057 5071 5105 1903
Size: 34 86 34 3014 34 3678
Fragments arranged by size:
3678 3014 86 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4772 5982 4772
Size: 1210 5670
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6880
BfrBI ATG'CAT
Cuts at: 4037 4133 4037
Size: 96 6784
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2415 2665 5491
Size: 107 122 71 631 605 743 250 2826
Cuts at: 5491 136
Size: 1525
Fragments arranged by size:
2826 1525 743 631 605 250 122 107
71
BglII A'GATC_T
Cuts at: 2232 4120 6875 2232
Size: 1888 2755 2237
Fragments arranged by size:
2755 2237 1888
BlpI GC'TnA_GC
Cuts at: 2264 4297 2264
Size: 2033 4847
Bme1580I G_kGCm'C
Cuts at: 1790 2616 2854 3278 4941 6097 1790
Size: 826 238 424 1663 1156 2573
Fragments arranged by size:
2573 1663 1156 826 424 238
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6880
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3608 4611 5569
Size: 416 53 83 399 24 2191 1003 958
Cuts at: 5569 442
Size: 1753
Fragments arranged by size:
2191 1753 1003 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2417 3565 2417
Size: 1148 5732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2111 2264 2318 2379 2552 2855 5540
Size: 836 36 153 54 61 173 303 2685
Cuts at: 5540 1239
Size: 2579
Fragments arranged by size:
2685 2579 836 303 173 153 61 54
36
Bpu10I CC'TnA_GC
Cuts at: 2661 3579 2661
Size: 918 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2522 5005 5870 6168 2522
Size: 2483 865 298 3234
Fragments arranged by size:
3234 2483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2388 5543 882
Size: 617 889 3155 2219
Fragments arranged by size:
3155 2219 889 617
BsaAI yAC'GTr
Cuts at: 493 1204 2086 4671 493
Size: 711 882 2585 2702
Fragments arranged by size:
2702 2585 882 711
BsaBI GATnn'nnATC
Cuts at: 6874 6874
Size: 6880
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2537 3749 4687 5069
Size: 53 83 186 936 1004 1212 938 382
Cuts at: 5069 5704 275
Size: 635 1451
Fragments arranged by size:
1451 1212 1004 938 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2456 5313 5711 5985 6132 6543 2456
Size: 2857 398 274 147 411 2793
Fragments arranged by size:
2857 2793 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4064 4094 1178
Size: 30 165 30 2661 30 3964
Fragments arranged by size:
3964 2661 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2381 2897 3170 3745 1186
Size: 991 204 516 273 575 4321
Fragments arranged by size:
4321 991 575 516 273 204
BseYI C'CCAG_C
Cuts at: 3265 3831 6083 6579 3265
Size: 566 2252 496 3566
Fragments arranged by size:
3566 2252 566 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3905 1163
Size: 573 2169 4138
Fragments arranged by size:
4138 2169 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4330 5091 6119 1540
Size: 275 186 2329 761 1028 2301
Fragments arranged by size:
2329 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 2616 3278 4941 5026 6097 2136
Size: 480 662 1663 85 1071 2919
Fragments arranged by size:
2919 1663 1071 662 480 85
BsmI GAATG_Cn'
Cuts at: 4039 4135 4391 4484 4039
Size: 96 256 93 6435
Fragments arranged by size:
6435 256 96 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2213 2528 2159
Size: 54 315 6511
Fragments arranged by size:
6511 315 54
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2616 2854 3278 4199 4941 5026
Size: 399 346 480 238 424 921 742 85
Cuts at: 5026 6097 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 921 742 480 424 399 346
238 85
BspEI T'CCGG_A
Cuts at: 6543 6543
Size: 6880
BspHI T'CATG_A
Cuts at: 2625 4769 6499 2625
Size: 2144 1730 3006
Fragments arranged by size:
3006 2144 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3087 3514 4111 4235 844
Size: 2243 427 597 124 3489
Fragments arranged by size:
3489 2243 597 427 124
BsrBI CCG'CTC
Cuts at: 1394 1823 4767 1394
Size: 429 2944 3507
Fragments arranged by size:
3507 2944 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3045 3586 5375 5549 65
Size: 1183 1797 541 1789 174 1396
Fragments arranged by size:
1797 1789 1396 1183 541 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 2205 2414 5524 5711 1700
Size: 110 326 69 209 3110 187 2869
Fragments arranged by size:
3110 2869 326 209 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 2556 96
Size: 2460 4420
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 4940 5952 4940
Size: 1012 5868
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2611 794
Size: 1817 5063
BstBI TT'CG_AA
Cuts at: 3192 5684 3192
Size: 2492 4388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6880
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6880
BstYI r'GATC_y
Cuts at: 1972 2232 3012 3616 4003 4120 4210 4577 4962
Size: 260 780 604 387 117 90 367 385
Cuts at: 4962 4979 6431 6517 6529 6875 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 780 604 387 385 367 346
260 117 90 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2973 2973
Size: 6880
BtgI C'CryG_G
Cuts at: 513 1065 2004 3003 3492 3876 513
Size: 552 939 999 489 384 3517
Fragments arranged by size:
3517 999 939 552 489 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2024 2030 3692 487
Size: 683 56 798 6 1662 3675
Fragments arranged by size:
3675 1662 798 683 56 6
BtsI GCAGTG_nn'
Cuts at: 4396 5190 5210 4396
Size: 794 20 6066
Fragments arranged by size:
6066 794 20
ClaI AT'CG_AT
Cuts at: 1554 3432 1554
Size: 1878 5002
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2482 2517 4155 4190 529
Size: 35 1918 35 1638 35 3219
Fragments arranged by size:
3219 1918 1638 35 35 35
DraI TTT'AAA
Cuts at: 4184 4184
Size: 6880
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3038 5887 809
Size: 507 1722 2849 1802
Fragments arranged by size:
2849 1802 1722 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2712 4327 5216
Size: 54 1425 50 275 18 882 1615 889
Cuts at: 5216 6845 8
Size: 1629 43
Fragments arranged by size:
1629 1615 1425 889 882 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4327 1537
Size: 275 2515 4090
Fragments arranged by size:
4090 2515 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2979 3714 3771 3885 4810 1353
Size: 1626 735 57 114 925 3423
Fragments arranged by size:
3423 1626 925 735 114 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 5467 5841 5987 387
Size: 1064 138 585 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 585 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2111 2201 2264 2318 2379 2552
Size: 393 443 36 90 63 54 61 173
Cuts at: 2552 2855 3048 3738 3920 4943 5540 6327 6595
Size: 303 193 690 182 1023 597 787 268
Cuts at: 6595 1239
Size: 1524
Fragments arranged by size:
1524 1023 787 690 597 443 393 303
268 193 182 173 90 63 61 54
36
EcoO109I rG'GnC_Cy
Cuts at: 2122 2566 2850 2851 3663 3895 2122
Size: 444 284 1 812 232 5107
Fragments arranged by size:
5107 812 444 284 232 1
EcoRI G'AATT_C
Cuts at: 4189 4567 4189
Size: 378 6502
EcoRV GAT'ATC
Cuts at: 3607 3607
Size: 6880
FspI TGC'GCA
Cuts at: 3051 5386 3051
Size: 2335 4545
HaeII r_GCGC'y
Cuts at: 1678 2156 6027 1678
Size: 478 3871 2531
Fragments arranged by size:
3871 2531 478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3208 3240 3603 3635 5521
Size: 32 135 32 1636 32 363 32 1886
Cuts at: 5521 5553 5595 5627 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1886 1636 363 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3076 4224 4470 669
Size: 988 281 1138 1148 246 3079
Fragments arranged by size:
3079 1148 1138 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4017 748
Size: 3269 3611
HpaI GTT'AAC
Cuts at: 4470 4470
Size: 6880
KpnI G_GTAC'C
Cuts at: 4205 4205
Size: 6880
MfeI C'AATT_G
Cuts at: 4479 4479
Size: 6880
MluI A'CGCG_T
Cuts at: 6756 6756
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 2088 5994 6178 1305
Size: 696 87 3906 184 2007
Fragments arranged by size:
3906 2007 696 184 87
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5058
Fragments arranged by size:
5058 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3179 3497 3503 4838 5197 5356
Size: 648 843 1170 318 6 1335 359 159
Cuts at: 5356 518
Size: 2042
Fragments arranged by size:
2042 1335 1170 843 648 359 318 159
6
MspA1I CmG'CkG
Cuts at: 1606 2053 2330 2608 2665 2891 2966 3317 3453
Size: 447 277 278 57 226 75 351 136
Cuts at: 3453 3560 4296 4973 6121 6366 1606
Size: 107 736 677 1148 245 2120
Fragments arranged by size:
2120 1148 736 677 447 351 278 277
245 226 136 107 75 57
NaeI GCC'GGC
Cuts at: 2416 2416
Size: 6880
NcoI C'CATG_G
Cuts at: 513 1065 2004 3003 3492 3876 513
Size: 552 939 999 489 384 3517
Fragments arranged by size:
3517 999 939 552 489 384
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6880
NgoMIV G'CCGG_C
Cuts at: 2414 2414
Size: 6880
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6880
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NsiI A_TGCA'T
Cuts at: 4039 4135 4039
Size: 96 6784
NspI r_CATG'y
Cuts at: 1132 1229 2755 3502 3977 4238 5783 1132
Size: 97 1526 747 475 261 1545 2229
Fragments arranged by size:
2229 1545 1526 747 475 261 97
PasI CC'CwG_GG
Cuts at: 1348 3760 1348
Size: 2412 4468
PciI A'CATG_T
Cuts at: 2751 5779 2751
Size: 3028 3852
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2155 3009 3103 3598 1108
Size: 1047 854 94 495 4390
Fragments arranged by size:
4390 1047 854 495 94
PfoI T'CCnGG_A
Cuts at: 2127 3015 2127
Size: 888 5992
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4949 4981 6462 6494 4949
Size: 32 1481 32 5335
Fragments arranged by size:
5335 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2566 2122
Size: 444 6436
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6880
PsiI TTA'TAA
Cuts at: 4450 4450
Size: 6880
PspOMI G'GGCC_C
Cuts at: 2850 2850
Size: 6880
PspXI vC'TCGA_Gb
Cuts at: 2898 2898
Size: 6880
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 6848
PstI C_TGCA'G
Cuts at: 830 2522 2607 4232 830
Size: 1692 85 1625 3478
Fragments arranged by size:
3478 1692 1625 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
PvuII CAG'CTG
Cuts at: 2053 2966 3560 2053
Size: 913 594 5373
Fragments arranged by size:
5373 913 594
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SbfI CC_TGCA'GG
Cuts at: 4232 4232
Size: 6880
ScaI AGT'ACT
Cuts at: 2549 5128 6840 2549
Size: 2579 1712 2589
Fragments arranged by size:
2589 2579 1712
SfcI C'TryA_G
Cuts at: 826 1045 2518 2603 3552 4228 5363 6044 6235
Size: 219 1473 85 949 676 1135 681 191
Cuts at: 6235 826
Size: 1471
Fragments arranged by size:
1473 1471 1135 949 681 676 219 191
85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 2434 4207 1872
Size: 562 1773 4545
Fragments arranged by size:
4545 1773 562
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 2898 3407 4984 5885 6147
Size: 197 926 558 397 509 1577 901 262
Cuts at: 6147 820
Size: 1553
Fragments arranged by size:
1577 1553 926 901 558 509 397 262
197
SnaBI TAC'GTA
Cuts at: 493 4671 493
Size: 4178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 4238 1132
Size: 3106 3774
SspI AAT'ATT
Cuts at: 5 52 4804 5
Size: 47 4752 2081
Fragments arranged by size:
4752 2081 47
StuI AGG'CCT
Cuts at: 1517 3284 1517
Size: 1767 5113
StyI C'CwwG_G
Cuts at: 513 1065 2004 2922 3003 3492 3658 3876 4319
Size: 552 939 918 81 489 166 218 443
Cuts at: 4319 513
Size: 3074
Fragments arranged by size:
3074 939 918 552 489 443 218 166
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2294 3590 3823 4907 4924 5077 5262
Size: 1255 714 1296 233 1084 17 153 185
Cuts at: 5262 5707 325
Size: 445 1498
Fragments arranged by size:
1498 1296 1255 1084 714 445 233 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 2556 3813
Size: 274 80 33 51 1443 570 9 1257
Cuts at: 3813 4163 5126 6838 96
Size: 350 963 1712 138
Fragments arranged by size:
1712 1443 1257 963 570 350 274 138
80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6840 6872 6840
Size: 32 6848
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6880
XbaI T'CTAG_A
Cuts at: 4136 4216 4136
Size: 80 6800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3874 3883 3874
Size: 9 6871
XhoI C'TCGA_G
Cuts at: 1943 2898 3407 1943
Size: 955 509 5416
Fragments arranged by size:
5416 955 509
XmaI C'CCGG_G
Cuts at: 1870 2432 4205 1870
Size: 562 1773 4545
Fragments arranged by size:
4545 1773 562
XmnI GAAnn'nnTTC
Cuts at: 5009 5009
Size: 6880
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4688 276
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI
NruI PacI PmlI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI