Restriction map for
pFN21AB9883
(Circular) (Six-base) MAPSORT of: pFN21AB9883.seq Check: 3022 from: 1 to: 6574
pFN21AB9883 6574 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 2287 4701 5074 1563
Size: 724 2414 373 3063
Fragments arranged by size:
3063 2414 724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1999 2753 2885 4637 6021 6289 1632
Size: 367 754 132 1752 1384 268 1917
Fragments arranged by size:
1917 1752 1384 754 367 268 132
AfeI AGC'GCT
Cuts at: 2870 2870
Size: 6574
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 5473 6450 1203
Size: 4270 977 1327
Fragments arranged by size:
4270 1327 977
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2723 3252 1184
Size: 1539 529 4506
Fragments arranged by size:
4506 1539 529
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3354 3386 3354
Size: 32 6542
AlwNI CAG_nnn'CTG
Cuts at: 2095 2731 3400 4317 5889 2095
Size: 636 669 917 1572 2780
Fragments arranged by size:
2780 1572 917 669 636
ApaLI G'TGCA_C
Cuts at: 3566 4631 5787 3566
Size: 1065 1156 4353
Fragments arranged by size:
4353 1156 1065
ApoI r'AATT_y
Cuts at: 1493 3516 3883 4109 4261 6370 1493
Size: 2023 367 226 152 2109 1697
Fragments arranged by size:
2109 2023 1697 367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 3025 3899 1870
Size: 73 193 889 874 4545
Fragments arranged by size:
4545 889 874 193 73
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2316 3135 3228 3895 5350
Size: 325 217 627 529 819 93 667 1455
Cuts at: 5350 618
Size: 1842
Fragments arranged by size:
1842 1455 819 667 627 529 325 217
93
BanII G_rGCy'C
Cuts at: 1391 2717 2978 3071 3281 3342 3609 3893 1391
Size: 1326 261 93 210 61 267 284 4072
Fragments arranged by size:
4072 1326 284 267 261 210 93 61
BbeI G_GCGC'C
Cuts at: 2320 3139 2320
Size: 819 5755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2574 928
Size: 971 675 4928
Fragments arranged by size:
4928 971 675
BbvCI CC'TCA_GC
Cuts at: 3333 3425 3333
Size: 92 6482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3105 3139 4765 4799 1903
Size: 34 1168 34 1626 34 3678
Fragments arranged by size:
3678 1626 1168 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2814 4466 5676 2814
Size: 1652 1210 3712
Fragments arranged by size:
3712 1652 1210
BclI T'GATC_A
Cuts at: 1548 3820 1548
Size: 2272 4302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2218 5185 136
Size: 107 122 71 631 605 546 2967 1525
Fragments arranged by size:
2967 1525 631 605 546 122 107 71
BglII A'GATC_T
Cuts at: 6569 6569
Size: 6574
BlpI GC'TnA_GC
Cuts at: 3991 3991
Size: 6574
Bme1580I G_kGCm'C
Cuts at: 1790 2592 3024 3570 4635 5791 1790
Size: 802 432 546 1065 1156 2573
Fragments arranged by size:
2573 1156 1065 802 546 432
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4305 5263 442
Size: 416 53 83 399 24 2888 958 1753
Fragments arranged by size:
2888 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3819 3819
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2680 2918 2954 3107 3766 5234 1239
Size: 1441 238 36 153 659 1468 2579
Fragments arranged by size:
2579 1468 1441 659 238 153 36
Bpu10I CC'TnA_GC
Cuts at: 2258 2847 2979 3275 3333 3425 2258
Size: 589 132 296 58 92 5407
Fragments arranged by size:
5407 589 296 132 92 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2789 4699 5564 5862 2789
Size: 1910 865 298 3501
Fragments arranged by size:
3501 1910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2505 5237 882
Size: 617 1006 2732 2219
Fragments arranged by size:
2732 2219 1006 617
BsaAI yAC'GTr
Cuts at: 493 1204 3565 4365 493
Size: 711 2361 800 2702
Fragments arranged by size:
2702 2361 800 711
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2317 3136 4381 4763
Size: 53 83 186 936 784 819 1245 382
Cuts at: 4763 5398 275
Size: 635 1451
Fragments arranged by size:
1451 1245 936 819 784 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2111 5007 5405 5679 5826 6237 2111
Size: 2896 398 274 147 411 2448
Fragments arranged by size:
2896 2448 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3153 3183 3263 3293 3653
Size: 30 165 30 1750 30 80 30 360
Cuts at: 3653 3683 1178
Size: 30 4069
Fragments arranged by size:
4069 1750 360 165 80 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2472 2873 3214 3350 3806 1186
Size: 1286 401 341 136 456 3954
Fragments arranged by size:
3954 1286 456 401 341 136
BseYI C'CCAG_C
Cuts at: 2028 2095 2168 2379 2391 2682 3840 5777 6273
Size: 67 73 211 12 291 1158 1937 496
Cuts at: 6273 2028
Size: 2329
Fragments arranged by size:
2329 1937 1158 496 291 211 73 67
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 3611 3740 1163
Size: 573 432 1443 129 3997
Fragments arranged by size:
3997 1443 573 432 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2399 4024 4785 5813 1540
Size: 275 186 398 1625 761 1028 2301
Fragments arranged by size:
2301 1625 1028 761 398 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 2231 3114 3281 3342 3570 3609 4635 4720
Size: 96 883 167 61 228 39 1026 85
Cuts at: 4720 5791 2135
Size: 1071 2918
Fragments arranged by size:
2918 1071 1026 883 228 167 96 85
61 39
BsmI GAATG_Cn'
Cuts at: 4085 4178 4085
Size: 93 6481
BsmBI CGTCTCn'nnnn_
Cuts at: 2807 2807
Size: 6574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2231 2592 2717 2978 3024 3071
Size: 399 345 96 361 125 261 46 47
Cuts at: 3071 3114 3281 3342 3570 3609 3893 4635 4720
Size: 43 167 61 228 39 284 742 85
Cuts at: 4720 5791 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 345 284 261
228 167 125 96 85 61 47 46
43 39
BspEI T'CCGG_A
Cuts at: 2111 6237 2111
Size: 4126 2448
BspHI T'CATG_A
Cuts at: 4463 6193 4463
Size: 1730 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3929 844
Size: 3085 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2578 2902 3412 4461 1394
Size: 429 755 324 510 1049 3507
Fragments arranged by size:
3507 1049 755 510 429 324
BsrDI GCAATG_nn'
Cuts at: 65 1248 5069 5243 65
Size: 1183 3821 174 1396
Fragments arranged by size:
3821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2301 2910 3036 5218 5405 1700
Size: 110 491 609 126 2182 187 2869
Fragments arranged by size:
2869 2182 609 491 187 126 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 3095 3097 3475 1925
Size: 1170 2 378 5024
Fragments arranged by size:
5024 1170 378 2
BssSI C'ACGA_G
Cuts at: 4634 5646 4634
Size: 1012 5562
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3187 3718 794
Size: 2393 531 3650
Fragments arranged by size:
3650 2393 531
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstYI r'GATC_y
Cuts at: 1972 2044 3543 3904 4271 4656 4673 6125 6211
Size: 72 1499 361 367 385 17 1452 86
Cuts at: 6211 6223 6569 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1499 1452 385 367 361 346 86
72 17 12
Bsu36I CC'TnA_GG
Cuts at: 3344 3401 3617 3774 3344
Size: 57 216 157 6144
Fragments arranged by size:
6144 216 157 57
BtgI C'CryG_G
Cuts at: 513 1065 2004 2060 2207 3240 513
Size: 552 939 56 147 1033 3847
Fragments arranged by size:
3847 1033 939 552 147 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2277 487
Size: 683 56 1051 4784
Fragments arranged by size:
4784 1051 683 56
BtsI GCAGTG_nn'
Cuts at: 2237 2529 2945 4090 4884 4904 2237
Size: 292 416 1145 794 20 3907
Fragments arranged by size:
3907 1145 794 416 292 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 3878 3878
Size: 6574
DraIII CAC_nnn'GTG
Cuts at: 2130 2507 2130
Size: 377 6197
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2544 5581 809
Size: 507 1228 3037 1802
Fragments arranged by size:
3037 1802 1228 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2396 4021 4910
Size: 54 1425 50 275 18 566 1625 889
Cuts at: 4910 6539 8
Size: 1629 43
Fragments arranged by size:
1629 1625 1425 889 566 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2396 4021 1537
Size: 275 584 1625 4090
Fragments arranged by size:
4090 1625 584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2630 2729 2742 3444 4504 1353
Size: 1277 99 13 702 1060 3423
Fragments arranged by size:
3423 1277 1060 702 99 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2085 3548 5161 5535 5681 387
Size: 1064 138 496 1463 1613 374 146 1280
Fragments arranged by size:
1613 1463 1280 1064 496 374 146 138
EcoICRI GAG'CTC
Cuts at: 3279 3340 3607 3279
Size: 61 267 6246
Fragments arranged by size:
6246 267 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2680 2753 2885 2918 2954 3107
Size: 393 367 681 73 132 33 36 153
Cuts at: 3107 3766 4637 5234 6021 6289 1239
Size: 659 871 597 787 268 1524
Fragments arranged by size:
1524 871 787 681 659 597 393 367
268 153 132 73 36 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3404 3404
Size: 6574
EcoO109I rG'GnC_Cy
Cuts at: 2277 2728 3307 3397 2277
Size: 451 579 90 5454
Fragments arranged by size:
5454 579 451 90
EcoRI G'AATT_C
Cuts at: 3516 3883 4261 3516
Size: 367 378 5829
Fragments arranged by size:
5829 378 367
FspI TGC'GCA
Cuts at: 3509 5080 3509
Size: 1571 5003
HaeII r_GCGC'y
Cuts at: 1678 2320 2872 2911 3139 3803 5721 1678
Size: 642 552 39 228 664 1918 2531
Fragments arranged by size:
2531 1918 664 642 552 228 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5215 5247 5289 5321 1373
Size: 32 135 32 3643 32 42 32 2626
Fragments arranged by size:
3643 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3918 4164 669
Size: 988 281 1980 246 3079
Fragments arranged by size:
3079 1980 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KasI G'GCGC_C
Cuts at: 2316 3135 2316
Size: 819 5755
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3571 5688 5872 1305
Size: 2266 2117 184 2007
Fragments arranged by size:
2266 2117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4752
Fragments arranged by size:
4752 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3234 4532 4891 5050 518
Size: 648 2068 1298 359 159 2042
Fragments arranged by size:
2068 2042 1298 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2092 2209 2383 2395 3193 3352 3376
Size: 456 30 117 174 12 798 159 24
Cuts at: 3376 3990 4667 5815 6060 1606
Size: 614 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 614 456 245 174
159 117 30 24 12
NaeI GCC'GGC
Cuts at: 2912 2912
Size: 6574
NarI GG'CG_CC
Cuts at: 2317 3136 2317
Size: 819 5755
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5083
Fragments arranged by size:
5083 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NgoMIV G'CCGG_C
Cuts at: 2910 2910
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 2396 4021 2396
Size: 1625 4949
NspI r_CATG'y
Cuts at: 1132 1229 3239 3932 5477 1132
Size: 97 2010 693 1545 2229
Fragments arranged by size:
2229 2010 1545 693 97
PasI CC'CwG_GG
Cuts at: 1348 2428 1348
Size: 1080 5494
PciI A'CATG_T
Cuts at: 5473 5473
Size: 6574
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2689 1108
Size: 1581 4993
PfoI T'CCnGG_A
Cuts at: 2291 3171 3261 3600 3753 3782 2291
Size: 880 90 339 153 29 5083
Fragments arranged by size:
5083 880 339 153 90 29
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PmlI CAC'GTG
Cuts at: 3565 3565
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 3307 2728
Size: 579 5995
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3854 3886 3854
Size: 32 6542
PstI C_TGCA'G
Cuts at: 830 2835 3192 3198 3926 830
Size: 2005 357 6 728 3478
Fragments arranged by size:
3478 2005 728 357 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2383 3193 3352 2383
Size: 810 159 5605
Fragments arranged by size:
5605 810 159
SacI G_AGCT'C
Cuts at: 3281 3342 3609 3281
Size: 61 267 6246
Fragments arranged by size:
6246 267 61
SacII CC_GC'GG
Cuts at: 2063 2210 2063
Size: 147 6427
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SapI GCTCTTCn'nnn_
Cuts at: 2729 3444 2729
Size: 715 5859
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SexAI A'CCwGG_T
Cuts at: 2652 2652
Size: 6574
SfcI C'TryA_G
Cuts at: 826 1045 2831 3188 3194 3922 5057 5738 5929
Size: 219 1786 357 6 728 1135 681 191
Cuts at: 5929 826
Size: 1471
Fragments arranged by size:
1786 1471 1135 728 681 357 219 191
6
SfoI GGC'GCC
Cuts at: 2318 3137 2318
Size: 819 5755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 2138 3027 3901 1872
Size: 266 889 874 4545
Fragments arranged by size:
4545 889 874 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2804 4678 5579 5841 820
Size: 197 926 861 1874 901 262 1553
Fragments arranged by size:
1874 1553 926 901 861 262 197
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3932 1132
Size: 2800 3774
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 2852 3779 1517
Size: 1335 927 4312
Fragments arranged by size:
4312 1335 927
StyI C'CwwG_G
Cuts at: 513 1065 2004 2688 3327 3733 4013 513
Size: 552 939 684 639 406 280 3074
Fragments arranged by size:
3074 939 684 639 552 406 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2738 4601 4618 4771 4956 5401 325
Size: 1255 1158 1863 17 153 185 445 1498
Fragments arranged by size:
1863 1498 1255 1158 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4820 6532 96
Size: 274 80 33 51 1443 2843 1712 138
Fragments arranged by size:
2843 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2137 2169 3023 3055 3653 3685 6534 6566 2137
Size: 32 854 32 598 32 2849 32 2145
Fragments arranged by size:
2849 2145 854 598 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2092 2092
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 2136 3025 3899 1870
Size: 266 889 874 4545
Fragments arranged by size:
4545 889 874 266
XmnI GAAnn'nnTTC
Cuts at: 4703 4703
Size: 6574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PspOMI PspXI RsrII SanDI SfiI SrfI SwaI