Restriction map for
pFN21AB9876
(Circular) (Six-base) MAPSORT of: pFN21AB9876.seq Check: 9379 from: 1 to: 6331
pFN21AB9876 6331 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3017 3017
Size: 6331
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4141 278
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3674 1937
Size: 1737 4594
Acc65I G'GTAC_C
Cuts at: 2572 3652 2572
Size: 1080 5251
AclI AA'CG_TT
Cuts at: 1563 4458 4831 1563
Size: 2895 373 3063
Fragments arranged by size:
3063 2895 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 2499 2580 3341 3477 4394 5778 6046
Size: 525 342 81 761 136 917 1384 268
Cuts at: 6046 1632
Size: 1917
Fragments arranged by size:
1917 1384 917 761 525 342 268 136
81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6134
AflIII A'CryG_T
Cuts at: 1203 2259 5230 6207 1203
Size: 1056 2971 977 1327
Fragments arranged by size:
2971 1327 1056 977
AgeI A'CCGG_T
Cuts at: 5162 5162
Size: 6331
AhdI GACnn_n'nnGTC
Cuts at: 2340 2442 3550 5060 2340
Size: 102 1108 1510 3611
Fragments arranged by size:
3611 1510 1108 102
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6331
AlwNI CAG_nnn'CTG
Cuts at: 4074 5646 4074
Size: 1572 4759
ApaI G_GGCC'C
Cuts at: 3090 3090
Size: 6331
ApaLI G'TGCA_C
Cuts at: 4388 5544 4388
Size: 1156 5175
ApoI r'AATT_y
Cuts at: 1493 2050 2164 2215 2966 3268 3640 3866 4018
Size: 557 114 51 751 302 372 226 152
Cuts at: 4018 6127 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 751 557 372 302 226 152
114 51
AseI AT'TA_AT
Cuts at: 160 1032 4885 160
Size: 872 3853 1606
Fragments arranged by size:
3853 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
AvaI C'yCGr_G
Cuts at: 1870 1943 3656 1870
Size: 73 1713 4545
Fragments arranged by size:
4545 1713 73
BamHI G'GATC_C
Cuts at: 3661 4028 3661
Size: 367 5964
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2572 3652 5107 618
Size: 325 217 627 785 1080 1455 1842
Fragments arranged by size:
1842 1455 1080 785 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2390 3090 3650 1391
Size: 999 700 560 4072
Fragments arranged by size:
4072 999 700 560
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 2598 2888 3003 3546 3584 928
Size: 971 453 246 290 115 543 38 3675
Fragments arranged by size:
3675 971 543 453 290 246 115 38
BbvCI CC'TCA_GC
Cuts at: 2906 2906
Size: 6331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4522 4556 1903
Size: 34 2585 34 3678
Fragments arranged by size:
3678 2585 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4223 5433 4223
Size: 1210 5121
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6331
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4942 136
Size: 107 122 71 631 605 3270 1525
Fragments arranged by size:
3270 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2095 6326 2095
Size: 4231 2100
BlpI GC'TnA_GC
Cuts at: 3748 3748
Size: 6331
Bme1580I G_kGCm'C
Cuts at: 1790 3090 4392 5548 1790
Size: 1300 1302 1156 2573
Fragments arranged by size:
2573 1302 1300 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6331
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2403 4062 5020
Size: 416 53 83 399 24 986 1659 958
Cuts at: 5020 442
Size: 1753
Fragments arranged by size:
1753 1659 986 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2487 4991 1239
Size: 1248 2504 2579
Fragments arranged by size:
2579 2504 1248
Bpu10I CC'TnA_GC
Cuts at: 2906 2906
Size: 6331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4456 5321 5619 4456
Size: 865 298 5168
Fragments arranged by size:
5168 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2467 4994 882
Size: 617 968 2527 2219
Fragments arranged by size:
2527 2219 968 617
BsaAI yAC'GTr
Cuts at: 493 1204 2260 4122 493
Size: 711 1056 1862 2702
Fragments arranged by size:
2702 1862 1056 711
BsaBI GATnn'nnATC
Cuts at: 2088 2094 6325 2088
Size: 6 4231 2094
Fragments arranged by size:
4231 2094 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4138 4520 5155 275
Size: 53 83 186 936 2605 382 635 1451
Fragments arranged by size:
2605 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4764 5162 5436 5583 5994 4764
Size: 398 274 147 411 5101
Fragments arranged by size:
5101 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2445 2475 1178
Size: 30 165 30 1042 30 5034
Fragments arranged by size:
5034 1042 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2484 1186
Size: 904 394 5033
Fragments arranged by size:
5033 904 394
BseYI C'CCAG_C
Cuts at: 2991 5534 6030 2991
Size: 2543 496 3292
Fragments arranged by size:
3292 2543 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5758
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2333 3781 4542 5570 1540
Size: 275 186 332 1448 761 1028 2301
Fragments arranged by size:
2301 1448 1028 761 332 275 186
BsiHKAI G_wGCw'C
Cuts at: 2390 3411 4392 4477 5548 2390
Size: 1021 981 85 1071 3173
Fragments arranged by size:
3173 1071 1021 981 85
BsmI GAATG_Cn'
Cuts at: 3842 3935 3842
Size: 93 6238
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2390 3090 3411 3650 4392 4477 5548
Size: 399 600 700 321 239 742 85 1071
Cuts at: 5548 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 700 600 399 321 239
85
BspEI T'CCGG_A
Cuts at: 5994 5994
Size: 6331
BspHI T'CATG_A
Cuts at: 4220 5950 4220
Size: 1730 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3017 3686 844
Size: 2173 669 3489
Fragments arranged by size:
3489 2173 669
BsrBI CCG'CTC
Cuts at: 1394 1823 4218 1394
Size: 429 2395 3507
Fragments arranged by size:
3507 2395 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2763 4826 5000 65
Size: 1183 1515 2063 174 1396
Fragments arranged by size:
2063 1515 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2308 3024 3372 4975 5162 1700
Size: 110 498 716 348 1603 187 2869
Fragments arranged by size:
2869 1603 716 498 348 187 110
BsrGI T'GTAC_A
Cuts at: 96 2122 3070 96
Size: 2026 948 3357
Fragments arranged by size:
3357 2026 948
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6331
BssSI C'ACGA_G
Cuts at: 3465 4391 5403 3465
Size: 926 1012 4393
Fragments arranged by size:
4393 1012 926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6331
BstBI TT'CG_AA
Cuts at: 5135 5135
Size: 6331
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6331
BstYI r'GATC_y
Cuts at: 1972 2089 2095 2640 2725 3661 4028 4413 4430
Size: 117 6 545 85 936 367 385 17
Cuts at: 4430 5882 5968 5980 6326 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 936 545 385 367 346 117
86 85 17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 2004 3081 513
Size: 552 939 1077 3763
Fragments arranged by size:
3763 1077 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3061 487
Size: 683 56 1835 3757
Fragments arranged by size:
3757 1835 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3847 4641 4661 3016
Size: 831 794 20 4686
Fragments arranged by size:
4686 831 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6296
DraI TTT'AAA
Cuts at: 2899 3436 3635 2899
Size: 537 199 5595
Fragments arranged by size:
5595 537 199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5338 809
Size: 507 4022 1802
Fragments arranged by size:
4022 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3778 4667 6296
Size: 54 1425 50 275 18 1948 889 1629
Cuts at: 6296 8
Size: 43
Fragments arranged by size:
1948 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3778 1537
Size: 275 1966 4090
Fragments arranged by size:
4090 1966 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4261 1353
Size: 2908 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4918 5292 5438 387
Size: 1064 138 3329 374 146 1280
Fragments arranged by size:
3329 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2388 2388
Size: 6331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2487 2499 2580 3341 3477 4394
Size: 393 525 330 12 81 761 136 917
Cuts at: 4394 4991 5778 6046 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 917 787 761 597 525 393 330
268 136 81 12
EcoO109I rG'GnC_Cy
Cuts at: 3086 3087 3136 3352 3086
Size: 1 49 216 6065
Fragments arranged by size:
6065 216 49 1
EcoRI G'AATT_C
Cuts at: 3640 4018 3640
Size: 378 5953
EcoRV GAT'ATC
Cuts at: 2014 2014
Size: 6331
FspI TGC'GCA
Cuts at: 4837 4837
Size: 6331
HaeII r_GCGC'y
Cuts at: 1678 5478 1678
Size: 3800 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2326 2347 2358 2428
Size: 32 135 32 743 11 21 11 70
Cuts at: 2428 2445 2460 2477 2785 2817 3201 3233 3536
Size: 17 15 17 308 32 384 32 303
Cuts at: 3536 3568 4972 5004 5046 5078 1373
Size: 32 1404 32 42 32 2626
Fragments arranged by size:
2626 1404 743 384 308 303 135 70
42 32 32 32 32 32 32 32
21 17 17 15 11 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3675 3921 669
Size: 988 281 1737 246 3079
Fragments arranged by size:
3079 1737 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6331
HpaI GTT'AAC
Cuts at: 3921 3921
Size: 6331
KpnI G_GTAC'C
Cuts at: 2576 3656 2576
Size: 1080 5251
MfeI C'AATT_G
Cuts at: 3930 3930
Size: 6331
MluI A'CGCG_T
Cuts at: 6207 6207
Size: 6331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2431 3311 5445 5629 1305
Size: 1126 880 2134 184 2007
Fragments arranged by size:
2134 2007 1126 880 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4509
Fragments arranged by size:
4509 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2130 3080 4289 4648 4807 518
Size: 648 964 950 1209 359 159 2042
Fragments arranged by size:
2042 1209 964 950 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2910 2991 3747 4424 5572 5817 1606
Size: 1304 81 756 677 1148 245 2120
Fragments arranged by size:
2120 1304 1148 756 677 245 81
NaeI GCC'GGC
Cuts at: 3374 3374
Size: 6331
NcoI C'CATG_G
Cuts at: 513 1065 2004 3081 513
Size: 552 939 1077 3763
Fragments arranged by size:
3763 1077 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6331
NgoMIV G'CCGG_C
Cuts at: 3372 3372
Size: 6331
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6331
NotI GC'GGCC_GC
Cuts at: 3778 3778
Size: 6331
NspI r_CATG'y
Cuts at: 1132 1229 2123 3689 5234 1132
Size: 97 894 1566 1545 2229
Fragments arranged by size:
2229 1566 1545 894 97
PasI CC'CwG_GG
Cuts at: 1348 2432 2567 1348
Size: 1084 135 5112
Fragments arranged by size:
5112 1084 135
PciI A'CATG_T
Cuts at: 5230 5230
Size: 6331
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6331
PmeI GTTT'AAAC
Cuts at: 3635 3635
Size: 6331
PmlI CAC'GTG
Cuts at: 2260 2260
Size: 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 5913 5945 4400
Size: 32 1481 32 4786
Fragments arranged by size:
4786 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3136 3352 3136
Size: 216 6115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6331
PsiI TTA'TAA
Cuts at: 2379 3901 2379
Size: 1522 4809
PspOMI G'GGCC_C
Cuts at: 3086 3086
Size: 6331
PstI C_TGCA'G
Cuts at: 830 3683 830
Size: 2853 3478
PvuI CG_AT'CG
Cuts at: 2001 2333 2001
Size: 332 5999
PvuII CAG'CTG
Cuts at: 2910 2991 2910
Size: 81 6250
SacI G_AGCT'C
Cuts at: 2390 2390
Size: 6331
SalI G'TCGA_C
Cuts at: 1936 3673 1936
Size: 1737 4594
SbfI CC_TGCA'GG
Cuts at: 3683 3683
Size: 6331
ScaI AGT'ACT
Cuts at: 4579 6291 4579
Size: 1712 4619
SexAI A'CCwGG_T
Cuts at: 2371 2371
Size: 6331
SfcI C'TryA_G
Cuts at: 826 1045 3050 3377 3679 4814 5495 5686 826
Size: 219 2005 327 302 1135 681 191 1471
Fragments arranged by size:
2005 1471 1135 681 327 302 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6331
SmaI CCC'GGG
Cuts at: 1872 3658 1872
Size: 1786 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4435 5336 5598 820
Size: 197 926 2492 901 262 1553
Fragments arranged by size:
2492 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4122 493
Size: 3629 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6331
SphI G_CATG'C
Cuts at: 1132 3689 1132
Size: 2557 3774
SspI AAT'ATT
Cuts at: 5 52 2932 4255 5
Size: 47 2880 1323 2081
Fragments arranged by size:
2880 2081 1323 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6331
StyI C'CwwG_G
Cuts at: 513 1065 2004 2684 3081 3417 3770 513
Size: 552 939 680 397 336 353 3074
Fragments arranged by size:
3074 939 680 552 397 353 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3205 4358 4375 4528 4713 5158 325
Size: 1255 1625 1153 17 153 185 445 1498
Fragments arranged by size:
1625 1498 1255 1153 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2122 3070 4577
Size: 274 80 33 51 1443 145 948 1507
Cuts at: 4577 6289 96
Size: 1712 138
Fragments arranged by size:
1712 1507 1443 948 274 145 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6291 6323 6291
Size: 32 6299
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6331
XbaI T'CTAG_A
Cuts at: 3667 3667
Size: 6331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2666 3167 2666
Size: 501 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6331
XmaI C'CCGG_G
Cuts at: 1870 3656 1870
Size: 1786 4545
XmnI GAAnn'nnTTC
Cuts at: 4460 4460
Size: 6331
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4139 276
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI