Restriction map for
pFN21AB9874
(Circular) (Six-base) MAPSORT of: pFN21AB9874.seq Check: 8931 from: 1 to: 6250
pFN21AB9874 6250 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2236 2236
Size: 6250
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4060 278
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 3593 1937
Size: 340 1316 4594
Fragments arranged by size:
4594 1316 340
Acc65I G'GTAC_C
Cuts at: 3571 3571
Size: 6250
AclI AA'CG_TT
Cuts at: 1563 4377 4750 1563
Size: 2814 373 3063
Fragments arranged by size:
3063 2814 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2849 3144 3527 4313 5697 5965 1632
Size: 516 701 295 383 786 1384 268 1917
Fragments arranged by size:
1917 1384 786 701 516 383 295 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6053
AflIII A'CryG_T
Cuts at: 1203 5149 6126 1203
Size: 3946 977 1327
Fragments arranged by size:
3946 1327 977
AgeI A'CCGG_T
Cuts at: 5081 5081
Size: 6250
AhdI GACnn_n'nnGTC
Cuts at: 4979 4979
Size: 6250
AleI CACnn'nnGTG
Cuts at: 1166 2474 2888 1166
Size: 1308 414 4528
Fragments arranged by size:
4528 1308 414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3355 3387 3355
Size: 32 6218
AlwNI CAG_nnn'CTG
Cuts at: 3462 3993 5565 3462
Size: 531 1572 4147
Fragments arranged by size:
4147 1572 531
ApaLI G'TGCA_C
Cuts at: 4307 5463 4307
Size: 1156 5094
ApoI r'AATT_y
Cuts at: 1493 2691 2765 2821 2905 3559 3785 3937 6046
Size: 1198 74 56 84 654 226 152 2109
Cuts at: 6046 1493
Size: 1697
Fragments arranged by size:
2109 1697 1198 654 226 152 84 74
56
AseI AT'TA_AT
Cuts at: 160 1032 4804 160
Size: 872 3772 1606
Fragments arranged by size:
3772 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 3575 1870
Size: 73 1632 4545
Fragments arranged by size:
4545 1632 73
AvrII C'CTAG_G
Cuts at: 2381 2701 2381
Size: 320 5930
BamHI G'GATC_C
Cuts at: 3580 3947 3580
Size: 367 5883
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3571 5026 618
Size: 325 217 627 1784 1455 1842
Fragments arranged by size:
1842 1784 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3569 1391
Size: 2178 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 928
Size: 971 354 4925
Fragments arranged by size:
4925 971 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4441 4475 1903
Size: 34 2504 34 3678
Fragments arranged by size:
3678 2504 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4142 5352 4142
Size: 1210 5040
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6250
BfrBI ATG'CAT
Cuts at: 2291 2964 2291
Size: 673 5577
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4861 136
Size: 107 122 71 631 605 3189 1525
Fragments arranged by size:
3189 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2073 3538 6245 2073
Size: 1465 2707 2078
Fragments arranged by size:
2707 2078 1465
BlpI GC'TnA_GC
Cuts at: 3667 3667
Size: 6250
Bme1580I G_kGCm'C
Cuts at: 1790 3356 4311 5467 1790
Size: 1566 955 1156 2573
Fragments arranged by size:
2573 1566 1156 955
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 3981 4939
Size: 416 53 83 399 24 828 1736 958
Cuts at: 4939 442
Size: 1753
Fragments arranged by size:
1753 1736 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2378 2441 4910 1239
Size: 818 321 63 2469 2579
Fragments arranged by size:
2579 2469 818 321 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2596 3317 4375 5240 5538 2333
Size: 263 721 1058 865 298 3045
Fragments arranged by size:
3045 1058 865 721 298 263
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4913 882
Size: 617 3414 2219
Fragments arranged by size:
3414 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3493 4041 493
Size: 711 2289 548 2702
Fragments arranged by size:
2702 2289 711 548
BsaBI GATnn'nnATC
Cuts at: 2856 6244 2856
Size: 3388 2862
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4057 4439 5074 275
Size: 53 83 186 936 2524 382 635 1451
Fragments arranged by size:
2524 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4683 5081 5355 5502 5913 4683
Size: 398 274 147 411 5020
Fragments arranged by size:
5020 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2354 2384 3355
Size: 30 165 30 636 30 285 30 971
Cuts at: 3355 3385 1178
Size: 30 4043
Fragments arranged by size:
4043 971 636 285 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2407 1186
Size: 1189 32 5029
Fragments arranged by size:
5029 1189 32
BseYI C'CCAG_C
Cuts at: 5453 5949 5453
Size: 496 5754
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5677
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3700 4461 5489 1540
Size: 275 186 1699 761 1028 2301
Fragments arranged by size:
2301 1699 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2813 4311 4396 5467 2813
Size: 1498 85 1071 3596
Fragments arranged by size:
3596 1498 1071 85
BsmI GAATG_Cn'
Cuts at: 2109 3761 3854 2109
Size: 1652 93 4505
Fragments arranged by size:
4505 1652 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2813 3356 3569 4311 4396 5467 1391
Size: 399 1023 543 213 742 85 1071 2174
Fragments arranged by size:
2174 1071 1023 742 543 399 213 85
BspEI T'CCGG_A
Cuts at: 5913 5913
Size: 6250
BspHI T'CATG_A
Cuts at: 2517 4139 5869 2517
Size: 1622 1730 2898
Fragments arranged by size:
2898 1730 1622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2236 3605 844
Size: 1392 1369 3489
Fragments arranged by size:
3489 1392 1369
BsrBI CCG'CTC
Cuts at: 1394 1823 3168 4137 1394
Size: 429 1345 969 3507
Fragments arranged by size:
3507 1345 969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4745 4919 65
Size: 1183 3497 174 1396
Fragments arranged by size:
3497 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4894 5081 1700
Size: 110 3084 187 2869
Fragments arranged by size:
3084 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6250
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6250
BssSI C'ACGA_G
Cuts at: 4310 5322 4310
Size: 1012 5238
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6250
BstBI TT'CG_AA
Cuts at: 2101 5054 2101
Size: 2953 3297
BstEII G'GTnAC_C
Cuts at: 1184 3095 3161 3312 3326 1184
Size: 1911 66 151 14 4108
Fragments arranged by size:
4108 1911 151 66 14
BstYI r'GATC_y
Cuts at: 1972 2073 3538 3580 3947 4332 4349 5801 5887
Size: 101 1465 42 367 385 17 1452 86
Cuts at: 5887 5899 6245 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1465 1452 385 367 346 101 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2572 3260 513
Size: 552 939 568 688 3503
Fragments arranged by size:
3503 939 688 568 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5511
Fragments arranged by size:
5511 683 56
BtsI GCAGTG_nn'
Cuts at: 2883 2895 3411 3766 4560 4580 2883
Size: 12 516 355 794 20 4553
Fragments arranged by size:
4553 794 516 355 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2047 2082 529
Size: 35 1483 35 4697
Fragments arranged by size:
4697 1483 35 35
DraI TTT'AAA
Cuts at: 2710 2904 3554 2710
Size: 194 650 5406
Fragments arranged by size:
5406 650 194
DraIII CAC_nnn'GTG
Cuts at: 3496 3496
Size: 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2606 5257 809
Size: 507 1290 2651 1802
Fragments arranged by size:
2651 1802 1290 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2575 3697 4586
Size: 54 1425 50 275 18 745 1122 889
Cuts at: 4586 6215 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1122 889 745 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3697 1537
Size: 275 1885 4090
Fragments arranged by size:
4090 1885 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 4180 1353
Size: 960 1867 3423
Fragments arranged by size:
3423 1867 960
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4837 5211 5357 387
Size: 1064 138 3248 374 146 1280
Fragments arranged by size:
3248 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2148 2378 2441 2849 3144 3527
Size: 393 425 91 230 63 408 295 383
Cuts at: 3527 4313 4910 5697 5965 1239
Size: 786 597 787 268 1524
Fragments arranged by size:
1524 787 786 597 425 408 393 383
295 268 230 91 63
EcoNI CCTnn'n_nnAGG
Cuts at: 3321 3378 3321
Size: 57 6193
EcoRI G'AATT_C
Cuts at: 2765 3559 3937 2765
Size: 794 378 5078
Fragments arranged by size:
5078 794 378
FspI TGC'GCA
Cuts at: 4756 4756
Size: 6250
HaeII r_GCGC'y
Cuts at: 1678 5397 1678
Size: 3719 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2323 2355 3377
Size: 32 135 32 563 32 156 32 1022
Cuts at: 3377 3409 4891 4923 4965 4997 1373
Size: 32 1482 32 42 32 2626
Fragments arranged by size:
2626 1482 1022 563 156 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3594 3840 669
Size: 988 281 1656 246 3079
Fragments arranged by size:
3079 1656 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6250
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KpnI G_GTAC'C
Cuts at: 3575 3575
Size: 6250
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 6126 6126
Size: 6250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2491 5364 5548 1305
Size: 1186 2873 184 2007
Fragments arranged by size:
2873 2007 1186 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2577 10
Size: 54 1425 343 745 3683
Fragments arranged by size:
3683 1425 745 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 2888 4208 4567 4726 518
Size: 648 1308 414 1320 359 159 2042
Fragments arranged by size:
2042 1320 1308 648 414 359 159
MspA1I CmG'CkG
Cuts at: 1606 2126 2419 3262 3666 4343 5491 5736 1606
Size: 520 293 843 404 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 677 520 404 293 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2572 513
Size: 552 939 568 4191
Fragments arranged by size:
4191 939 568 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6250
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6250
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NsiI A_TGCA'T
Cuts at: 2293 2966 2293
Size: 673 5577
NspI r_CATG'y
Cuts at: 1132 1229 3608 5153 1132
Size: 97 2379 1545 2229
Fragments arranged by size:
2379 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6250
PciI A'CATG_T
Cuts at: 5149 5149
Size: 6250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3106 1108
Size: 1998 4252
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3355 3387 4319 4351 5832 5864 3137
Size: 32 186 32 932 32 1481 32 3523
Fragments arranged by size:
3523 1481 932 186 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 2856 3820 2856
Size: 964 5286
PstI C_TGCA'G
Cuts at: 830 2189 2247 2890 3602 830
Size: 1359 58 643 712 3478
Fragments arranged by size:
3478 1359 712 643 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
PvuII CAG'CTG
Cuts at: 2126 2419 2126
Size: 293 5957
SacII CC_GC'GG
Cuts at: 3263 3263
Size: 6250
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 3016 4498 6210 3016
Size: 1482 1712 3056
Fragments arranged by size:
3056 1712 1482
SfcI C'TryA_G
Cuts at: 826 1045 2185 2243 2655 2886 3186 3291 3598
Size: 219 1140 58 412 231 300 105 307
Cuts at: 3598 4733 5414 5605 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1140 1135 681 412 307 300 231
219 191 105 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 3577 1872
Size: 1705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2611 3332 4354 5255 5517
Size: 197 926 369 299 721 1022 901 262
Cuts at: 5517 820
Size: 1553
Fragments arranged by size:
1553 1022 926 901 721 369 299 262
197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 3017 152
Size: 2865 3385
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SspI AAT'ATT
Cuts at: 5 52 2634 3075 4174 5
Size: 47 2582 441 1099 2081
Fragments arranged by size:
2582 2081 1099 441 47
StuI AGG'CCT
Cuts at: 1517 2210 1517
Size: 693 5557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2023 2381 2572 2701 3689 513
Size: 552 939 19 358 191 129 988 3074
Fragments arranged by size:
3074 988 939 552 358 191 129 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 4277 4294 4447 4632 5077 325
Size: 1255 525 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 525 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2986 3014 4496
Size: 274 80 33 51 1443 1009 28 1482
Cuts at: 4496 6208 96
Size: 1712 138
Fragments arranged by size:
1712 1482 1443 1009 274 138 80 51
33 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 3482 3514 6210 6242 2039
Size: 32 1411 32 2696 32 2047
Fragments arranged by size:
2696 2047 1411 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6250
XbaI T'CTAG_A
Cuts at: 3155 3586 3155
Size: 431 5819
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 3575 1870
Size: 1705 4545
XmnI GAAnn'nnTTC
Cuts at: 4379 4379
Size: 6250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4058 276
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI