Restriction map for
pFN21AB9871
(Circular) (Six-base) MAPSORT of: pFN21AB9871.seq Check: 4180 from: 1 to: 6175
pFN21AB9871 6175 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2823 3005 3141 3231 2823
Size: 182 136 90 5767
Fragments arranged by size:
5767 182 136 90
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2776 4238 5622 5890 1632
Size: 1144 1462 1384 268 1917
Fragments arranged by size:
1917 1462 1384 1144 268
AfeI AGC'GCT
Cuts at: 2929 3191 3248 3275 2929
Size: 262 57 27 5829
Fragments arranged by size:
5829 262 57 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 2507 1166
Size: 1341 4834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3083 1184
Size: 1899 4276
AlwNI CAG_nnn'CTG
Cuts at: 3918 5490 3918
Size: 1572 4603
ApaI G_GGCC'C
Cuts at: 3038 3038
Size: 6175
ApaLI G'TGCA_C
Cuts at: 2498 4232 5388 2498
Size: 1734 1156 3285
Fragments arranged by size:
3285 1734 1156
ApoI r'AATT_y
Cuts at: 1493 3484 3710 3862 5971 1493
Size: 1991 226 152 2109 1697
Fragments arranged by size:
2109 1991 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 2584 3085 3352 3446 3452 3500 1870
Size: 73 641 501 267 94 6 48 4545
Fragments arranged by size:
4545 641 501 267 94 73 48 6
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2621 2801 2941 3219 3456
Size: 325 217 627 834 180 140 278 237
Cuts at: 3456 3496 4951 618
Size: 40 1455 1842
Fragments arranged by size:
1842 1455 834 627 325 278 237 217
180 140 40
BanII G_rGCy'C
Cuts at: 1391 2615 2724 2918 2957 3038 3353 3453 3494
Size: 1224 109 194 39 81 315 100 41
Cuts at: 3494 1391
Size: 4072
Fragments arranged by size:
4072 1224 315 194 109 100 81 41
39
BbeI G_GCGC'C
Cuts at: 2625 2805 2945 2625
Size: 180 140 5855
Fragments arranged by size:
5855 180 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3317 928
Size: 971 1418 3786
Fragments arranged by size:
3786 1418 971
BbvCI CC'TCA_GC
Cuts at: 2968 3296 2968
Size: 328 5847
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4366 4400 1903
Size: 34 50 34 2345 34 3678
Fragments arranged by size:
3678 2345 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 5277 4067
Size: 1210 4965
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6170 6170
Size: 6175
BlpI GC'TnA_GC
Cuts at: 2819 3592 2819
Size: 773 5402
Bme1580I G_kGCm'C
Cuts at: 1790 2502 3038 3459 4236 5392 1790
Size: 712 536 421 777 1156 2573
Fragments arranged by size:
2573 1156 777 712 536 421
BmgBI CAC'GTC
Cuts at: 1318 2641 2662 1318
Size: 1323 21 4831
Fragments arranged by size:
4831 1323 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3906 4864 442
Size: 416 53 83 399 24 2489 958 1753
Fragments arranged by size:
2489 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2167 2272 2293 2338 2611 4835 1239
Size: 928 105 21 45 273 2224 2579
Fragments arranged by size:
2579 2224 928 273 105 45 21
Bpu10I CC'TnA_GC
Cuts at: 2968 3296 2968
Size: 328 5847
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2143 3107 4300 5165 5463 2143
Size: 964 1193 865 298 2855
Fragments arranged by size:
2855 1193 964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4838 882
Size: 617 3339 2219
Fragments arranged by size:
3339 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3307 3966 493
Size: 711 2103 659 2702
Fragments arranged by size:
2702 2103 711 659
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2622 2802 2942 3982
Size: 53 83 186 936 1089 180 140 1040
Cuts at: 3982 4364 4999 275
Size: 382 635 1451
Fragments arranged by size:
1451 1089 1040 936 635 382 186 180
140 83 53
BsaWI w'CCGG_w
Cuts at: 4608 5006 5280 5427 5838 4608
Size: 398 274 147 411 4945
Fragments arranged by size:
4945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5950
Fragments arranged by size:
5950 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2101 2437 2797 2956 1186
Size: 858 57 336 360 159 4405
Fragments arranged by size:
4405 858 360 336 159 57
BseYI C'CCAG_C
Cuts at: 2039 2930 2947 3031 5378 5874 2039
Size: 891 17 84 2347 496 2340
Fragments arranged by size:
2347 2340 891 496 84 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 2687 2720 3053 1163
Size: 573 948 3 33 333 4285
Fragments arranged by size:
4285 948 573 333 33 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2220 2619 2989 3625 4386 5414
Size: 275 186 219 399 370 636 761 1028
Cuts at: 5414 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 636 399 370 275 219
186
BsiHKAI G_wGCw'C
Cuts at: 2502 3396 4236 4321 5392 2502
Size: 894 840 85 1071 3285
Fragments arranged by size:
3285 1071 894 840 85
BsiWI C'GTAC_G
Cuts at: 2556 2556
Size: 6175
BsmI GAATG_Cn'
Cuts at: 3686 3779 3686
Size: 93 6082
BsmBI CGTCTCn'nnnn_
Cuts at: 2052 2052
Size: 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2502 2615 2724 2918 2957 3038 3353
Size: 399 712 113 109 194 39 81 315
Cuts at: 3353 3396 3453 3459 3494 4236 4321 5392 1391
Size: 43 57 6 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 399 315 194 113
109 85 81 57 43 39 35 6
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 3005 3141 3231 3530 844
Size: 1979 182 136 90 299 3489
Fragments arranged by size:
3489 1979 299 182 136 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2746 3028 3154 4062 1394
Size: 429 923 282 126 908 3507
Fragments arranged by size:
3507 923 908 429 282 126
BsrDI GCAATG_nn'
Cuts at: 65 1248 4670 4844 65
Size: 1183 3422 174 1396
Fragments arranged by size:
3422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2479 2618 2648 2903 3236 4819 5006
Size: 110 669 139 30 255 333 1583 187
Cuts at: 5006 1700
Size: 2869
Fragments arranged by size:
2869 1583 669 333 255 187 139 110
30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 4235 5247 4235
Size: 1012 5163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6175
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6175
BstYI r'GATC_y
Cuts at: 1972 3505 3872 4257 4274 5726 5812 5824 6170
Size: 1533 367 385 17 1452 86 12 346
Cuts at: 6170 1972
Size: 1977
Fragments arranged by size:
1977 1533 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2220 2232 2241 2534 3402 513
Size: 552 1155 12 9 293 868 3286
Fragments arranged by size:
3286 1155 868 552 293 12 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5436
Fragments arranged by size:
5436 683 56
BtsI GCAGTG_nn'
Cuts at: 3691 4485 4505 3691
Size: 794 20 5361
Fragments arranged by size:
5361 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 3479 3479
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2616 2901
Size: 54 1425 50 275 18 387 399 285
Cuts at: 2901 2986 3622 4511 6140 8
Size: 85 636 889 1629 43
Fragments arranged by size:
1629 1425 889 636 399 387 285 275
85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2217 2616 2986 3622 1537
Size: 275 405 399 370 636 4090
Fragments arranged by size:
4090 636 405 399 370 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2077 2125 4105 1353
Size: 724 48 1980 3423
Fragments arranged by size:
3423 1980 724 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2116 4762 5136 5282 387
Size: 1064 138 527 2646 374 146 1280
Fragments arranged by size:
2646 1280 1064 527 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 2272 2293 2338 2611 2776 4238
Size: 393 535 105 21 45 273 165 1462
Cuts at: 4238 4835 5622 5890 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1462 787 597 535 393 273 268
165 105 45 21
EcoO109I rG'GnC_Cy
Cuts at: 2178 2392 3035 3064 2178
Size: 214 643 29 5289
Fragments arranged by size:
5289 643 214 29
EcoRI G'AATT_C
Cuts at: 3484 3862 3484
Size: 378 5797
FspI TGC'GCA
Cuts at: 3284 4681 3284
Size: 1397 4778
HaeII r_GCGC'y
Cuts at: 1678 2295 2319 2625 2805 2931 2945 3193 3250
Size: 617 24 306 180 126 14 248 57
Cuts at: 3250 3277 3402 5322 1678
Size: 27 125 1920 2531
Fragments arranged by size:
2531 1920 617 306 248 180 126 125
57 27 24 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4816 4848 4890 4922 1373
Size: 32 135 32 3244 32 42 32 2626
Fragments arranged by size:
3244 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3519 3765 669
Size: 988 281 1581 246 3079
Fragments arranged by size:
3079 1581 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2161 748
Size: 1413 4762
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KasI G'GCGC_C
Cuts at: 2621 2801 2941 2621
Size: 180 140 5855
Fragments arranged by size:
5855 180 140
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 3228 5289 5473 1305
Size: 975 948 2061 184 2007
Fragments arranged by size:
2061 2007 975 948 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4353
Fragments arranged by size:
4353 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2507 4133 4492 4651 518
Size: 648 1341 1626 359 159 2042
Fragments arranged by size:
2042 1626 1341 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2133 2222 2234 2243 2268 2415 2536 2631
Size: 527 89 12 9 25 147 121 95
Cuts at: 2631 2799 2899 2947 3075 3217 3360 3404 3591
Size: 168 100 48 128 142 143 44 187
Cuts at: 3591 4268 5416 5661 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 527 245 187 168 147
143 142 128 121 100 95 89 48
44 25 12 9
NaeI GCC'GGC
Cuts at: 2620 2650 2905 2620
Size: 30 255 5890
Fragments arranged by size:
5890 255 30
NarI GG'CG_CC
Cuts at: 2622 2802 2942 2622
Size: 180 140 5855
Fragments arranged by size:
5855 180 140
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5623
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NgoMIV G'CCGG_C
Cuts at: 2618 2648 2903 2618
Size: 30 255 5890
Fragments arranged by size:
5890 255 30
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 2217 3622 2217
Size: 1405 4770
NspI r_CATG'y
Cuts at: 1132 1229 3533 5078 1132
Size: 97 2304 1545 2229
Fragments arranged by size:
2304 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2634 1108
Size: 1526 4649
PfoI T'CCnGG_A
Cuts at: 2183 2183
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PmlI CAC'GTG
Cuts at: 3307 3307
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2180 2212 4244 4276 5757 5789 2180
Size: 32 2032 32 1481 32 2566
Fragments arranged by size:
2566 2032 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PspOMI G'GGCC_C
Cuts at: 3034 3034
Size: 6175
PstI C_TGCA'G
Cuts at: 830 2523 3216 3441 3527 830
Size: 1693 693 225 86 3478
Fragments arranged by size:
3478 1693 693 225 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2133 2268 2631 3075 2133
Size: 135 363 444 5233
Fragments arranged by size:
5233 444 363 135
SacII CC_GC'GG
Cuts at: 2223 2235 2244 2537 3405 2223
Size: 12 9 293 868 4993
Fragments arranged by size:
4993 868 293 12 9
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SapI GCTCTTCn'nnn_
Cuts at: 2077 2077
Size: 6175
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2519 3212 3437 3523 4658 5339 5530
Size: 219 1474 693 225 86 1135 681 191
Cuts at: 5530 826
Size: 1471
Fragments arranged by size:
1474 1471 1135 693 681 225 219 191
86
SfoI GGC'GCC
Cuts at: 2623 2803 2943 2623
Size: 180 140 5855
Fragments arranged by size:
5855 180 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 3236 1700
Size: 1536 4639
SmaI CCC'GGG
Cuts at: 1872 2586 3087 3454 3502 1872
Size: 714 501 367 48 4545
Fragments arranged by size:
4545 714 501 367 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2158 3122 4279 5180 5442 820
Size: 197 926 215 964 1157 901 262 1553
Fragments arranged by size:
1553 1157 964 926 901 262 215 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SrfI GCCC'GGGC
Cuts at: 3454 3454
Size: 6175
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 2775 1517
Size: 1258 4917
StyI C'CwwG_G
Cuts at: 513 1065 2493 3614 513
Size: 552 1428 1121 3074
Fragments arranged by size:
3074 1428 1121 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2582 4202 4219 4372 4557 5002 325
Size: 1255 1002 1620 17 153 185 445 1498
Fragments arranged by size:
1620 1498 1255 1002 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4421 6133 96
Size: 274 80 33 51 1443 2444 1712 138
Fragments arranged by size:
2444 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6135 6167 6135
Size: 32 6143
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 3511 3511
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 2584 3085 3452 3500 1870
Size: 714 501 367 48 4545
Fragments arranged by size:
4545 714 501 367 48
XmnI GAAnn'nnTTC
Cuts at: 3248 4304 3248
Size: 1056 5119
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SwaI XcmI