Restriction map for
pFN21AB9870
(Circular) (Six-base) MAPSORT of: pFN21AB9870.seq Check: 8818 from: 1 to: 6136
pFN21AB9870 6136 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2223 2223
Size: 6136
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2349 3033 3335 3946
Size: 53 83 186 936 813 684 302 611
Cuts at: 3946 278
Size: 2468
Fragments arranged by size:
2468 936 813 684 611 302 186 83
53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4199 5583 5851 1632
Size: 2567 1384 268 1917
Fragments arranged by size:
2567 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 3152 1166
Size: 1986 4150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 6104
AlwNI CAG_nnn'CTG
Cuts at: 2065 3121 3879 5451 2065
Size: 1056 758 1572 2750
Fragments arranged by size:
2750 1572 1056 758
ApaI G_GGCC'C
Cuts at: 2118 2118
Size: 6136
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 3445 3671 3823 5932 1493
Size: 1952 226 152 2109 1697
Fragments arranged by size:
2109 1952 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 3461 1870
Size: 73 95 1423 4545
Fragments arranged by size:
4545 1423 95 73
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2101 3457 4912 618
Size: 325 217 627 240 74 1356 1455 1842
Fragments arranged by size:
1842 1455 1356 627 325 240 217 74
BanII G_rGCy'C
Cuts at: 1391 2093 2118 2634 3183 3455 1391
Size: 702 25 516 549 272 4072
Fragments arranged by size:
4072 702 549 516 272 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2230 2908 3025 3199 928
Size: 971 331 678 117 174 3865
Fragments arranged by size:
3865 971 678 331 174 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2624 2658 4327 4361 1903
Size: 34 50 34 603 34 1669 34 3678
Fragments arranged by size:
3678 1669 603 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 2904 1548
Size: 1356 4780
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2113 2312 4747
Size: 107 122 71 631 605 441 199 2435
Cuts at: 4747 136
Size: 1525
Fragments arranged by size:
2435 1525 631 605 441 199 122 107
71
BglII A'GATC_T
Cuts at: 3321 6131 3321
Size: 2810 3326
BlpI GC'TnA_GC
Cuts at: 3553 3553
Size: 6136
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2118 4197 5353 1790
Size: 314 14 2079 1156 2573
Fragments arranged by size:
2573 2079 1156 314 14
BmgBI CAC'GTC
Cuts at: 1318 2638 1318
Size: 1320 4816
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2120 2431 3867
Size: 416 53 83 399 24 703 311 1436
Cuts at: 3867 4825 442
Size: 958 1753
Fragments arranged by size:
1753 1436 958 703 416 399 311 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2416 2644 2810 4796 1239
Size: 1177 228 166 1986 2579
Fragments arranged by size:
2579 1986 1177 228 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2873 4261 5126 5424 2873
Size: 1388 865 298 3585
Fragments arranged by size:
3585 1388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2870 3011 4799 882
Size: 617 1371 141 1788 2219
Fragments arranged by size:
2219 1788 1371 617 141
BsaAI yAC'GTr
Cuts at: 493 1204 3927 493
Size: 711 2723 2702
Fragments arranged by size:
2723 2702 711
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 2606 3030 3332
Size: 53 83 186 936 813 260 424 302
Cuts at: 3332 3943 4325 4960 275
Size: 611 382 635 1451
Fragments arranged by size:
1451 936 813 635 611 424 382 302
260 186 83 53
BsaWI w'CCGG_w
Cuts at: 2527 4569 4967 5241 5388 5799 2527
Size: 2042 398 274 147 411 2864
Fragments arranged by size:
2864 2042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2642 2672 3125 3155 1178
Size: 30 165 30 1239 30 453 30 4159
Fragments arranged by size:
4159 1239 453 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 3049 3305 3308 1186
Size: 1444 419 256 3 4014
Fragments arranged by size:
4014 1444 419 256 3
BseYI C'CCAG_C
Cuts at: 2170 3048 3114 3121 5339 5835 2170
Size: 878 66 7 2218 496 2471
Fragments arranged by size:
2471 2218 878 496 66 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2786 3148 1163
Size: 573 1050 362 4151
Fragments arranged by size:
4151 1050 573 362
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2997 3310 3586 4347 5375 1540
Size: 275 186 996 313 276 761 1028 2301
Fragments arranged by size:
2301 1028 996 761 313 276 275 186
BsiHKAI G_wGCw'C
Cuts at: 2375 2808 3183 4197 4282 5353 2375
Size: 433 375 1014 85 1071 3158
Fragments arranged by size:
3158 1071 1014 433 375 85
BsmI GAATG_Cn'
Cuts at: 2647 3647 3740 2647
Size: 1000 93 5043
Fragments arranged by size:
5043 1000 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3152 3152
Size: 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2104 2118 2375 2634 2808 3183
Size: 399 303 11 14 257 259 174 375
Cuts at: 3183 3455 4197 4282 5353 1391
Size: 272 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 303 272 259
257 174 85 14 11
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 2724 4025 5755 2724
Size: 1301 1730 3105
Fragments arranged by size:
3105 1730 1301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 2589 3491 844
Size: 1379 366 902 3489
Fragments arranged by size:
3489 1379 902 366
BsrBI CCG'CTC
Cuts at: 1394 1823 4023 1394
Size: 429 2200 3507
Fragments arranged by size:
3507 2200 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3181 4631 4805 65
Size: 1183 1933 1450 174 1396
Fragments arranged by size:
1933 1450 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2315 2996 3151 4780 4967 1700
Size: 110 505 681 155 1629 187 2869
Fragments arranged by size:
2869 1629 681 505 187 155 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 2218 2838 2857 1925
Size: 293 620 19 5204
Fragments arranged by size:
5204 620 293 19
BssSI C'ACGA_G
Cuts at: 4196 5208 4196
Size: 1012 5124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6136
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 2128 3344 1184
Size: 944 1216 3976
Fragments arranged by size:
3976 1216 944
BstYI r'GATC_y
Cuts at: 1972 2769 3321 3466 3833 4218 4235 5687 5773
Size: 797 552 145 367 385 17 1452 86
Cuts at: 5773 5785 6131 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 797 552 385 367 346 145
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2082 513
Size: 552 1017 4567
Fragments arranged by size:
4567 1017 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2740 487
Size: 683 56 1514 3883
Fragments arranged by size:
3883 1514 683 56
BtsI GCAGTG_nn'
Cuts at: 2376 2977 3652 4446 4466 2376
Size: 601 675 794 20 4046
Fragments arranged by size:
4046 794 675 601 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3035 5143 809
Size: 507 1719 2108 1802
Fragments arranged by size:
2108 1802 1719 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2313 2595
Size: 54 1425 50 275 18 423 60 282
Cuts at: 2595 2994 3583 4472 6101 8
Size: 399 589 889 1629 43
Fragments arranged by size:
1629 1425 889 589 423 399 282 275
60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2994 3583 1537
Size: 275 1182 589 4090
Fragments arranged by size:
4090 1182 589 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2692 4066 1353
Size: 1339 1374 3423
Fragments arranged by size:
3423 1374 1339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4723 5097 5243 387
Size: 1064 138 3134 374 146 1280
Fragments arranged by size:
3134 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3181 3181
Size: 6136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2416 2644 2810 4199 4796 5583 5851
Size: 393 784 228 166 1389 597 787 268
Cuts at: 5851 1239
Size: 1524
Fragments arranged by size:
1524 1389 787 784 597 393 268 228
166
EcoO109I rG'GnC_Cy
Cuts at: 2114 2115 2436 2987 3299 2114
Size: 1 321 551 312 4951
Fragments arranged by size:
4951 551 321 312 1
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FspI TGC'GCA
Cuts at: 2191 4642 2191
Size: 2451 3685
HaeII r_GCGC'y
Cuts at: 1678 2279 5283 1678
Size: 601 3004 2531
Fragments arranged by size:
3004 2531 601
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4777 4809 4851 4883 1373
Size: 32 135 32 3205 32 42 32 2626
Fragments arranged by size:
3205 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6136
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5250 5434 1305
Size: 3945 184 2007
Fragments arranged by size:
3945 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2597 10
Size: 54 1425 343 765 3549
Fragments arranged by size:
3549 1425 765 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2729 3152 3239 4094 4453 4612
Size: 648 1242 321 423 87 855 359 159
Cuts at: 4612 518
Size: 2042
Fragments arranged by size:
2042 1242 855 648 423 359 321 159
87
MspA1I CmG'CkG
Cuts at: 1606 2065 3118 3227 3552 4229 5377 5622 1606
Size: 459 1053 109 325 677 1148 245 2120
Fragments arranged by size:
2120 1148 1053 677 459 325 245 109
NaeI GCC'GGC
Cuts at: 2998 2998
Size: 6136
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5584
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NgoMIV G'CCGG_C
Cuts at: 2996 2996
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 3316 3494 5039 1132
Size: 97 2087 178 1545 2229
Fragments arranged by size:
2229 2087 1545 178 97
PasI CC'CwG_GG
Cuts at: 1348 2610 1348
Size: 1262 4874
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 3121 1108
Size: 957 1056 4123
Fragments arranged by size:
4123 1056 957
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 4205 4237 5718 5750 2642
Size: 32 1531 32 1481 32 3028
Fragments arranged by size:
3028 1531 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3299 3299
Size: 6136
PshAI GACnn'nnGTC
Cuts at: 1613 2234 1613
Size: 621 5515
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PspOMI G'GGCC_C
Cuts at: 2114 2114
Size: 6136
PstI C_TGCA'G
Cuts at: 830 2972 3488 830
Size: 2142 516 3478
Fragments arranged by size:
3478 2142 516
PvuI CG_AT'CG
Cuts at: 2001 3310 2001
Size: 1309 4827
PvuII CAG'CTG
Cuts at: 2065 3118 3227 2065
Size: 1053 109 4974
Fragments arranged by size:
4974 1053 109
SacI G_AGCT'C
Cuts at: 3183 3183
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SfcI C'TryA_G
Cuts at: 826 1045 2968 3484 4619 5300 5491 826
Size: 219 1923 516 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 516 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 3151 1700
Size: 1451 4685
SmaI CCC'GGG
Cuts at: 1872 2040 3463 1872
Size: 168 1423 4545
Fragments arranged by size:
4545 1423 168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2888 4240 5141 5403 820
Size: 197 926 945 1352 901 262 1553
Fragments arranged by size:
1553 1352 945 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6136
StyI C'CwwG_G
Cuts at: 513 1065 2475 2880 3575 513
Size: 552 1410 405 695 3074
Fragments arranged by size:
3074 1410 695 552 405
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 4163 4180 4333 4518 4963 325
Size: 1255 761 1822 17 153 185 445 1498
Fragments arranged by size:
1822 1498 1255 761 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4382 6094 96
Size: 274 80 33 51 1443 2405 1712 138
Fragments arranged by size:
2405 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 6096 6128 3125
Size: 32 2939 32 3133
Fragments arranged by size:
3133 2939 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2503 2896 3203 1316
Size: 1187 393 307 4249
Fragments arranged by size:
4249 1187 393 307
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 2038 3461 1870
Size: 168 1423 4545
Fragments arranged by size:
4545 1423 168
XmnI GAAnn'nnTTC
Cuts at: 3428 4265 3428
Size: 837 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2347 3031 3333 3944
Size: 53 83 186 936 813 684 302 611
Cuts at: 3944 276
Size: 2468
Fragments arranged by size:
2468 936 813 684 611 302 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI