Restriction map for
pFN21AB9867
(Circular) (Six-base) MAPSORT of: pFN21AB9867.seq Check: 9977 from: 1 to: 6043
pFN21AB9867 6043 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2172 2172
Size: 6043
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3294 3853 278
Size: 53 83 186 936 1758 559 2468
Fragments arranged by size:
2468 1758 936 559 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 1937
Size: 1449 4594
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2837 4106 5490 5758 1632
Size: 1205 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 1205 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 2946 4942 5919 1203
Size: 1743 1996 977 1327
Fragments arranged by size:
1996 1743 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 2255 3786 5358 2255
Size: 1531 1572 2940
Fragments arranged by size:
2940 1572 1531
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 3368 1870
Size: 73 1425 4545
Fragments arranged by size:
4545 1425 73
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3364 4819 618
Size: 325 217 627 1577 1455 1842
Fragments arranged by size:
1842 1577 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3194 3362 1391
Size: 1803 168 4072
Fragments arranged by size:
4072 1803 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2825 928
Size: 971 926 4146
Fragments arranged by size:
4146 971 926
BbvCI CC'TCA_GC
Cuts at: 2573 2573
Size: 6043
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4234 4268 1903
Size: 34 2297 34 3678
Fragments arranged by size:
3678 2297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2084 3935 5145 2084
Size: 1851 1210 2982
Fragments arranged by size:
2982 1851 1210
BclI T'GATC_A
Cuts at: 1548 2352 2433 2832 1548
Size: 804 81 399 4759
Fragments arranged by size:
4759 804 399 81
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4654 136
Size: 107 122 71 631 605 2982 1525
Fragments arranged by size:
2982 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2212 6038 2212
Size: 3826 2217
BlpI GC'TnA_GC
Cuts at: 2486 3460 2486
Size: 974 5069
Bme1580I G_kGCm'C
Cuts at: 1790 4104 5260 1790
Size: 2314 1156 2573
Fragments arranged by size:
2573 2314 1156
BmgBI CAC'GTC
Cuts at: 1318 2947 1318
Size: 1629 4414
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2049 2471 3774
Size: 416 53 83 399 24 632 422 1303
Cuts at: 3774 4732 442
Size: 958 1753
Fragments arranged by size:
1753 1303 958 632 422 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2365 2551 3320 4703 1239
Size: 1126 186 769 1383 2579
Fragments arranged by size:
2579 1383 1126 769 186
Bpu10I CC'TnA_GC
Cuts at: 2573 2573
Size: 6043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 4706 882
Size: 617 1041 2166 2219
Fragments arranged by size:
2219 2166 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 3834 493
Size: 711 2630 2702
Fragments arranged by size:
2702 2630 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3291 3850 4232 4867
Size: 53 83 186 936 1758 559 382 635
Cuts at: 4867 275
Size: 1451
Fragments arranged by size:
1758 1451 936 635 559 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2391 2770 4476 4874 5148 5295 5706 2391
Size: 379 1706 398 274 147 411 2728
Fragments arranged by size:
2728 1706 411 398 379 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 3236 3266 1178
Size: 30 165 30 1146 30 657 30 3955
Fragments arranged by size:
3955 1146 657 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3184 3230 1186
Size: 1998 46 3999
Fragments arranged by size:
3999 1998 46
BseYI C'CCAG_C
Cuts at: 3256 5246 5742 3256
Size: 1990 496 3557
Fragments arranged by size:
3557 1990 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 1163
Size: 573 765 4705
Fragments arranged by size:
4705 765 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3493 4254 5282 1540
Size: 275 186 1492 761 1028 2301
Fragments arranged by size:
2301 1492 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2324 4104 4189 5260 2324
Size: 1780 85 1071 3107
Fragments arranged by size:
3107 1780 1071 85
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
BsmBI CGTCTCn'nnnn_
Cuts at: 2903 3074 3298 2903
Size: 171 224 5648
Fragments arranged by size:
5648 224 171
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2324 3194 3362 4104 4189 5260 1391
Size: 399 534 870 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 870 742 534 399 168 85
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 2631 3127 3932 5662 2631
Size: 496 805 1730 3012
Fragments arranged by size:
3012 1730 805 496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 3398 844
Size: 1328 1226 3489
Fragments arranged by size:
3489 1328 1226
BsrBI CCG'CTC
Cuts at: 1394 1823 3930 1394
Size: 429 2107 3507
Fragments arranged by size:
3507 2107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2685 4538 4712 65
Size: 1183 1437 1853 174 1396
Fragments arranged by size:
1853 1437 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4687 4874 1700
Size: 110 2877 187 2869
Fragments arranged by size:
2877 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 3135 4103 5115 3135
Size: 968 1012 4063
Fragments arranged by size:
4063 1012 968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 2176 1184
Size: 992 5051
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 2539 3241 2119
Size: 420 702 4921
Fragments arranged by size:
4921 702 420
BstYI r'GATC_y
Cuts at: 1972 2212 2652 3306 3373 3740 4125 4142 5594
Size: 240 440 654 67 367 385 17 1452
Cuts at: 5594 5680 5692 6038 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 654 440 385 367 346 240
86 67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2028 513
Size: 552 963 4528
Fragments arranged by size:
4528 963 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3125 3214 487
Size: 683 56 1899 89 3316
Fragments arranged by size:
3316 1899 683 89 56
BtsI GCAGTG_nn'
Cuts at: 2079 2256 2325 3559 4353 4373 2079
Size: 177 69 1234 794 20 3749
Fragments arranged by size:
3749 1234 794 177 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3290 5050 809
Size: 507 1974 1760 1802
Fragments arranged by size:
1974 1802 1760 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2625 3490
Size: 54 1425 50 275 18 393 402 865
Cuts at: 3490 4379 6008 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 865 402 393 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3490 1537
Size: 275 1678 4090
Fragments arranged by size:
4090 1678 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3973 1353
Size: 2620 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2365 2551 2837 3320 4106 4703 5490
Size: 393 733 186 286 483 786 597 787
Cuts at: 5490 5758 1239
Size: 268 1524
Fragments arranged by size:
1524 787 786 733 597 483 393 286
268 186
EcoNI CCTnn'n_nnAGG
Cuts at: 2647 2698 3209 2647
Size: 51 511 5481
Fragments arranged by size:
5481 511 51
EcoO109I rG'GnC_Cy
Cuts at: 2283 3202 2283
Size: 919 5124
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2809 2440
Size: 369 5674
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 5190 1678
Size: 3512 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3129 3161 3236 3268 4684
Size: 32 135 32 1557 32 75 32 1416
Cuts at: 4684 4716 4758 4790 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1557 1416 135 75 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2608 748
Size: 1860 4183
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2182 2958 3334 5157 5341 1305
Size: 877 776 376 1823 184 2007
Fragments arranged by size:
2007 1823 877 776 376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2627 10
Size: 54 1425 343 795 3426
Fragments arranged by size:
3426 1425 795 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 2477 2621 2840 4001 4360 4519
Size: 648 1134 177 144 219 1161 359 159
Cuts at: 4519 518
Size: 2042
Fragments arranged by size:
2042 1161 1134 648 359 219 177 159
144
MspA1I CmG'CkG
Cuts at: 1606 2490 3224 3459 4136 5284 5529 1606
Size: 884 734 235 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 734 677 245 235
NcoI C'CATG_G
Cuts at: 513 1065 2028 513
Size: 552 963 4528
Fragments arranged by size:
4528 963 552
NdeI CA'TA_TG
Cuts at: 387 2584 387
Size: 2197 3846
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 3185 3401 4946 1132
Size: 97 1956 216 1545 2229
Fragments arranged by size:
2229 1956 1545 216 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6043
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6043
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2283 3202 2283
Size: 919 5124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PstI C_TGCA'G
Cuts at: 830 3395 830
Size: 2565 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2490 3224 2490
Size: 734 5309
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 2656 3046 3391 4526 5207 5398 826
Size: 219 1611 390 345 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 681 390 345 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 3370 1872
Size: 1498 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 5048 5310 820
Size: 197 926 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6043
StyI C'CwwG_G
Cuts at: 513 1065 2028 2172 2199 2415 2694 3150 3240
Size: 552 963 144 27 216 279 456 90
Cuts at: 3240 3482 513
Size: 242 3074
Fragments arranged by size:
3074 963 552 456 279 242 216 144
90 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2290 2828 2952 3256 3351 4070 4087
Size: 1255 710 538 124 304 95 719 17
Cuts at: 4087 4240 4425 4870 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 719 710 538 445 304 185
153 124 95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2458 4289 6001
Size: 274 80 33 51 1443 481 1831 1712
Cuts at: 6001 96
Size: 138
Fragments arranged by size:
1831 1712 1443 481 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2153 2185 6003 6035 2153
Size: 32 3818 32 2161
Fragments arranged by size:
3818 2161 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2452 2824 1316
Size: 1136 372 4535
Fragments arranged by size:
4535 1136 372
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 3368 1870
Size: 1498 4545
XmnI GAAnn'nnTTC
Cuts at: 4172 4172
Size: 6043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3292 3851 276
Size: 53 83 186 936 1758 559 2468
Fragments arranged by size:
2468 1758 936 559 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI