Restriction map for
pFN21AB9866
(Circular) (Six-base) MAPSORT of: pFN21AB9866.seq Check: 4033 from: 1 to: 6010
pFN21AB9866 6010 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2171 2973 2171
Size: 802 5208
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 2549 4073 5457 5725 1632
Size: 737 180 1524 1384 268 1917
Fragments arranged by size:
1917 1524 1384 737 268 180
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 2133 4909 5886 1203
Size: 930 2776 977 1327
Fragments arranged by size:
2776 1327 977 930
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2126 2847 1184
Size: 942 721 4347
Fragments arranged by size:
4347 942 721
AlwNI CAG_nnn'CTG
Cuts at: 3753 5325 3753
Size: 1572 4438
ApaI G_GGCC'C
Cuts at: 2875 2875
Size: 6010
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 3319 3545 3697 5806 1493
Size: 1826 226 152 2109 1697
Fragments arranged by size:
2109 1826 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2867 3176 3335 1870
Size: 73 924 309 159 4545
Fragments arranged by size:
4545 924 309 159 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3115 3331 4786 618
Size: 325 217 627 1328 216 1455 1842
Fragments arranged by size:
1842 1455 1328 627 325 217 216
BanII G_rGCy'C
Cuts at: 1391 2363 2875 3329 1391
Size: 972 512 454 4072
Fragments arranged by size:
4072 972 512 454
BbeI G_GCGC'C
Cuts at: 3119 3119
Size: 6010
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2035 2446 2609 928
Size: 971 136 411 163 4329
Fragments arranged by size:
4329 971 411 163 136
BbvCI CC'TCA_GC
Cuts at: 3102 3102
Size: 6010
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4201 4235 1903
Size: 34 2264 34 3678
Fragments arranged by size:
3678 2264 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2718 2735 2996
Size: 107 122 71 631 605 1046 17 261
Cuts at: 2996 3069 3260 3269 4621 136
Size: 73 191 9 1352 1525
Fragments arranged by size:
1525 1352 1046 631 605 261 191 122
107 73 71 17 9
BglII A'GATC_T
Cuts at: 2667 6005 2667
Size: 3338 2672
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 2875 4071 5227 1790
Size: 1085 1196 1156 2573
Fragments arranged by size:
2573 1196 1156 1085
BmgBI CAC'GTC
Cuts at: 1318 2134 1318
Size: 816 5194
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2995 3741 4699
Size: 416 53 83 399 24 1578 746 958
Cuts at: 4699 442
Size: 1753
Fragments arranged by size:
1753 1578 958 746 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2906 1056
Size: 1850 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 2572 2684 3170 4670 1239
Size: 796 537 112 486 1500 2579
Fragments arranged by size:
2579 1500 796 537 486 112
Bpu10I CC'TnA_GC
Cuts at: 3102 3102
Size: 6010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2678 3008 4135 5000 5298 2678
Size: 330 1127 865 298 3390
Fragments arranged by size:
3390 1127 865 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3234 4673 882
Size: 617 1735 1439 2219
Fragments arranged by size:
2219 1735 1439 617
BsaAI yAC'GTr
Cuts at: 493 1204 2290 3801 493
Size: 711 1086 1511 2702
Fragments arranged by size:
2702 1511 1086 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2727 3116 3817 4199
Size: 53 83 186 936 1194 389 701 382
Cuts at: 4199 4834 275
Size: 635 1451
Fragments arranged by size:
1451 1194 936 701 635 389 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3292 4443 4841 5115 5262 5673 3292
Size: 1151 398 274 147 411 3629
Fragments arranged by size:
3629 1151 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 1178
Size: 30 165 30 717 30 5038
Fragments arranged by size:
5038 717 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2351 2354 3194 3197 3200 1186
Size: 1162 3 3 840 3 3 3996
Fragments arranged by size:
3996 1162 840 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2113 2172 2363 2743 2774 3059 5213 5709 2113
Size: 59 191 380 31 285 2154 496 2414
Fragments arranged by size:
2414 2154 496 380 285 191 59 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 1163
Size: 573 543 4894
Fragments arranged by size:
4894 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2410 2551 2786 3003 3460 4221
Size: 275 186 409 141 235 217 457 761
Cuts at: 4221 5249 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 457 409 275 235 217
186 141
BsiHKAI G_wGCw'C
Cuts at: 2055 4071 4156 5227 2055
Size: 2016 85 1071 2838
Fragments arranged by size:
2838 2016 1071 85
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2363 2875 3329 4071 4156 5227
Size: 399 265 308 512 454 742 85 1071
Cuts at: 5227 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 512 454 399 308 265
85
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2171 2973 3365 844
Size: 1327 802 392 3489
Fragments arranged by size:
3489 1327 802 392
BsrBI CCG'CTC
Cuts at: 1394 1823 2536 3007 3138 3897 1394
Size: 429 713 471 131 759 3507
Fragments arranged by size:
3507 759 713 471 429 131
BsrDI GCAATG_nn'
Cuts at: 65 1248 4505 4679 65
Size: 1183 3257 174 1396
Fragments arranged by size:
3257 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2498 4654 4841 1700
Size: 110 688 2156 187 2869
Fragments arranged by size:
2869 2156 688 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 2918 1925
Size: 993 5017
BssSI C'ACGA_G
Cuts at: 3095 4070 5082 3095
Size: 975 1012 4023
Fragments arranged by size:
4023 1012 975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 794
Size: 1508 4502
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 2572 2572
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 2667 3340 3707 4092 4109 5561 5647 5659
Size: 695 673 367 385 17 1452 86 12
Cuts at: 5659 6005 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 695 673 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2121 2410 2595 2856 2886
Size: 552 939 54 63 289 185 261 30
Cuts at: 2886 3204 513
Size: 318 3319
Fragments arranged by size:
3319 939 552 318 289 261 185 63
54 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2157 2479 487
Size: 683 56 931 322 4018
Fragments arranged by size:
4018 931 683 322 56
BtsI GCAGTG_nn'
Cuts at: 3526 4320 4340 3526
Size: 794 20 5196
Fragments arranged by size:
5196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DraIII CAC_nnn'GTG
Cuts at: 2302 2302
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5017 809
Size: 507 3701 1802
Fragments arranged by size:
3701 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2427 2500 2783
Size: 54 1425 50 275 18 597 73 283
Cuts at: 2783 2953 3000 3303 3457 4346 5975 8
Size: 170 47 303 154 889 1629 43
Fragments arranged by size:
1629 1425 889 597 303 283 275 170
154 73 54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2783 3000 3457 1537
Size: 275 971 217 457 4090
Fragments arranged by size:
4090 971 457 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2307 2345 3940 1353
Size: 954 38 1595 3423
Fragments arranged by size:
3423 1595 954 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2141 2926 4597 4971 5117 387
Size: 1064 138 552 785 1671 374 146 1280
Fragments arranged by size:
1671 1280 1064 785 552 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2369 2549 2572 2684 3170 4073
Size: 393 403 334 180 23 112 486 903
Cuts at: 4073 4670 5457 5725 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 903 787 597 486 403 393 334
268 180 112 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2843 2912 2843
Size: 69 5941
EcoO109I rG'GnC_Cy
Cuts at: 2208 2737 2871 2872 3171 2208
Size: 529 134 1 299 5047
Fragments arranged by size:
5047 529 299 134 1
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 6010
FspI TGC'GCA
Cuts at: 2306 2423 2823 4516 2306
Size: 117 400 1693 3800
Fragments arranged by size:
3800 1693 400 117
FspAI rTGC'GCAy
Cuts at: 2306 2423 2823 2306
Size: 117 400 5493
Fragments arranged by size:
5493 400 117
HaeII r_GCGC'y
Cuts at: 1678 2243 2438 3119 3162 5157 1678
Size: 565 195 681 43 1995 2531
Fragments arranged by size:
2531 1995 681 565 195 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2378 2410 2740
Size: 32 135 32 563 32 211 32 330
Cuts at: 2740 2772 4651 4683 4725 4757 1373
Size: 32 1879 32 42 32 2626
Fragments arranged by size:
2626 1879 563 330 211 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KasI G'GCGC_C
Cuts at: 3115 3115
Size: 6010
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5124 5308 1305
Size: 3819 184 2007
Fragments arranged by size:
3819 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3305 10
Size: 54 1425 343 1473 2715
Fragments arranged by size:
2715 1473 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2255 2621 3968 4327 4486 518
Size: 648 942 147 366 1347 359 159 2042
Fragments arranged by size:
2042 1347 942 648 366 359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2123 2347 2412 2544 2553 2743 2831 2888
Size: 517 224 65 132 9 190 88 57
Cuts at: 2888 3206 3279 3426 4103 5251 5496 1606
Size: 318 73 147 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 517 318 245 224 190
147 132 88 73 65 57 9
NaeI GCC'GGC
Cuts at: 2500 2500
Size: 6010
NarI GG'CG_CC
Cuts at: 3116 3116
Size: 6010
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NgoMIV G'CCGG_C
Cuts at: 2498 2498
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 2902 1052
Size: 1850 4160
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2662 3368 4913 1132
Size: 97 1433 706 1545 2229
Fragments arranged by size:
2229 1545 1433 706 97
PasI CC'CwG_GG
Cuts at: 1348 2839 1348
Size: 1491 4519
PciI A'CATG_T
Cuts at: 4909 4909
Size: 6010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6010
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PmlI CAC'GTG
Cuts at: 2290 2290
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3171 3171
Size: 6010
PshAI GACnn'nnGTC
Cuts at: 1613 2450 3246 1613
Size: 837 796 4377
Fragments arranged by size:
4377 837 796
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PspOMI G'GGCC_C
Cuts at: 2871 2871
Size: 6010
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2183 2215 2183
Size: 32 5978
PstI C_TGCA'G
Cuts at: 830 2247 3055 3362 830
Size: 1417 808 307 3478
Fragments arranged by size:
3478 1417 808 307
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2347 2347
Size: 6010
SacII CC_GC'GG
Cuts at: 2124 2413 2889 3207 2124
Size: 289 476 318 4927
Fragments arranged by size:
4927 476 318 289
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SanDI GG'GwC_CC
Cuts at: 3171 3171
Size: 6010
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 2507 4258 5970 2507
Size: 1751 1712 2547
Fragments arranged by size:
2547 1751 1712
SfcI C'TryA_G
Cuts at: 826 1045 2243 3051 3358 4493 5174 5365 826
Size: 219 1198 808 307 1135 681 191 1471
Fragments arranged by size:
1471 1198 1135 808 681 307 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3260 3269 3260
Size: 9 6001
SfoI GGC'GCC
Cuts at: 3117 3117
Size: 6010
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 2869 3337 1872
Size: 997 468 4545
Fragments arranged by size:
4545 997 468
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2693 3023 4114 5015 5277 820
Size: 197 926 750 330 1091 901 262 1553
Fragments arranged by size:
1553 1091 926 901 750 330 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2004 2370 3449 513
Size: 552 939 366 1079 3074
Fragments arranged by size:
3074 1079 939 552 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2186 2927 4037 4054 4207 4392 4837
Size: 1255 606 741 1110 17 153 185 445
Cuts at: 4837 325
Size: 1498
Fragments arranged by size:
1498 1255 1110 741 606 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 4256 5968
Size: 274 80 33 51 1443 528 1751 1712
Cuts at: 5968 96
Size: 138
Fragments arranged by size:
1751 1712 1443 528 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2120 2152 5970 6002 2120
Size: 32 3818 32 2128
Fragments arranged by size:
3818 2128 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 2867 3335 1870
Size: 997 468 4545
Fragments arranged by size:
4545 997 468
XmnI GAAnn'nnTTC
Cuts at: 4139 4139
Size: 6010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI NruI NsiI
PacI PfoI PspXI RsrII SacI SapI SexAI SrfI
SwaI XcmI