Restriction map for
pFN21AB9862
(Circular) (Six-base) MAPSORT of: pFN21AB9862.seq Check: 5935 from: 1 to: 5953
pFN21AB9862 5953 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2173 2655 3074 2173
Size: 482 419 5052
Fragments arranged by size:
5052 482 419
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3296 1937
Size: 1359 4594
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2238 2325 4016 5400 5668 1632
Size: 606 87 1691 1384 268 1917
Fragments arranged by size:
1917 1691 1384 606 268 87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 2574 4852 5829 1203
Size: 1371 2278 977 1327
Fragments arranged by size:
2278 1371 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5953
AlwNI CAG_nnn'CTG
Cuts at: 3025 3696 5268 3025
Size: 671 1572 3710
Fragments arranged by size:
3710 1572 671
ApaI G_GGCC'C
Cuts at: 2722 2722
Size: 5953
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 3262 3488 3640 5749 1493
Size: 1769 226 152 2109 1697
Fragments arranged by size:
2109 1769 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2242 2635 2743 2768 3278 1870
Size: 73 271 28 393 108 25 510 4545
Fragments arranged by size:
4545 510 393 271 108 73 28 25
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2264 2278 2845 2884 3227
Size: 325 217 627 477 14 567 39 343
Cuts at: 3227 3274 4729 618
Size: 47 1455 1842
Fragments arranged by size:
1842 1455 627 567 477 343 325 217
47 39 14
BanII G_rGCy'C
Cuts at: 1391 2644 2722 3272 1391
Size: 1253 78 550 4072
Fragments arranged by size:
4072 1253 550 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 2118 2130 928
Size: 971 180 39 12 4751
Fragments arranged by size:
4751 971 180 39 12
BbvCI CC'TCA_GC
Cuts at: 2918 2918
Size: 5953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2789 2823 4144 4178 1903
Size: 34 852 34 1321 34 3678
Fragments arranged by size:
3678 1321 852 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2532 3244 4564
Size: 107 122 71 631 605 860 712 1320
Cuts at: 4564 136
Size: 1525
Fragments arranged by size:
1525 1320 860 712 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5948 5948
Size: 5953
BlpI GC'TnA_GC
Cuts at: 2022 2396 3370 2022
Size: 374 974 4605
Fragments arranged by size:
4605 974 374
Bme1580I G_kGCm'C
Cuts at: 1790 2281 2722 2848 3230 4014 5170 1790
Size: 491 441 126 382 784 1156 2573
Fragments arranged by size:
2573 1156 784 491 441 382 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5953
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 4642 442
Size: 416 53 83 399 24 2267 958 1753
Fragments arranged by size:
2267 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2569 3055 4613 1239
Size: 914 416 486 1558 2579
Fragments arranged by size:
2579 1558 914 486 416
Bpu10I CC'TnA_GC
Cuts at: 2918 2918
Size: 5953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4078 4943 5241 4078
Size: 865 298 4790
Fragments arranged by size:
4790 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4616 882
Size: 617 3117 2219
Fragments arranged by size:
3117 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2776 2947 3760 4142
Size: 53 83 186 936 1243 171 813 382
Cuts at: 4142 4777 275
Size: 635 1451
Fragments arranged by size:
1451 1243 936 813 635 382 186 171
83 53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 1178
Size: 30 165 30 1146 30 4552
Fragments arranged by size:
4552 1146 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2144 2147 2150 2944 1186
Size: 928 30 3 3 794 4195
Fragments arranged by size:
4195 928 794 30 3 3
BseYI C'CCAG_C
Cuts at: 3219 5156 5652 3219
Size: 1937 496 3520
Fragments arranged by size:
3520 1937 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2658 2672 2777 1163
Size: 573 922 14 105 4339
Fragments arranged by size:
4339 922 573 105 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3047 3403 4164 5192 1540
Size: 275 186 1046 356 761 1028 2301
Fragments arranged by size:
2301 1046 1028 761 356 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 4014 4099 5170 2223
Size: 1791 85 1071 3006
Fragments arranged by size:
3006 1791 1071 85
BsmI GAATG_Cn'
Cuts at: 3464 3557 3464
Size: 93 5860
BsmBI CGTCTCn'nnnn_
Cuts at: 2767 2767
Size: 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2281 2644 2722 2848 3230 3272
Size: 399 433 58 363 78 126 382 42
Cuts at: 3272 4014 4099 5170 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 382 363 126
85 78 58 42
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2019 2173 2655 2677 3074 3308 844
Size: 1175 154 482 22 397 234 3489
Fragments arranged by size:
3489 1175 482 397 234 154 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3013 3840 1394
Size: 429 1190 827 3507
Fragments arranged by size:
3507 1190 827 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4448 4622 65
Size: 1183 3200 174 1396
Fragments arranged by size:
3200 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2320 4597 4784 1700
Size: 110 510 2277 187 2869
Fragments arranged by size:
2869 2277 510 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 4013 5025 4013
Size: 1012 4941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5953
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 2508 3283 3650 4035 4052 5504 5590 5602
Size: 536 775 367 385 17 1452 86 12
Cuts at: 5602 5948 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 775 536 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2358 513
Size: 552 1293 4108
Fragments arranged by size:
4108 1293 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2329 487
Size: 683 56 1103 4111
Fragments arranged by size:
4111 1103 683 56
BtsI GCAGTG_nn'
Cuts at: 3469 4263 4283 3469
Size: 794 20 5139
Fragments arranged by size:
5139 794 20
ClaI AT'CG_AT
Cuts at: 1554 2085 1554
Size: 531 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2422 3400
Size: 54 1425 50 275 18 531 61 978
Cuts at: 3400 4289 5918 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 978 889 531 275 61 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2811 3883 1353
Size: 1458 1072 3423
Fragments arranged by size:
3423 1458 1072
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2462 2978 3117 4540 4914 5060
Size: 1064 138 873 516 139 1423 374 146
Cuts at: 5060 387
Size: 1280
Fragments arranged by size:
1423 1280 1064 873 516 374 146 139
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2238 2325 2569 3055 4016 4613
Size: 393 521 85 87 244 486 961 597
Cuts at: 4613 5400 5668 1239
Size: 787 268 1524
Fragments arranged by size:
1524 961 787 597 521 486 393 268
244 87 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2096
Size: 5953
EcoO109I rG'GnC_Cy
Cuts at: 2705 2718 2719 2748 2705
Size: 13 1 29 5910
Fragments arranged by size:
5910 29 13 1
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
FspI TGC'GCA
Cuts at: 3029 4459 3029
Size: 1430 4523
HaeII r_GCGC'y
Cuts at: 1678 2029 2389 2623 3250 5100 1678
Size: 351 360 234 627 1850 2531
Fragments arranged by size:
2531 1850 627 360 351 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2208 2240 2549 2581 4594
Size: 32 135 32 636 32 309 32 2013
Cuts at: 4594 4626 4668 4700 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2013 636 309 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3297 3543 669
Size: 988 281 1359 246 3079
Fragments arranged by size:
3079 1359 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5067 5251 1305
Size: 3762 184 2007
Fragments arranged by size:
3762 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3600
Fragments arranged by size:
3600 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 3911 4270 4429 518
Size: 648 1191 1554 359 159 2042
Fragments arranged by size:
2042 1554 1191 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2400 3106 3136 3369 4046 5194 5439 1606
Size: 794 706 30 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 706 677 245 233 30
NaeI GCC'GGC
Cuts at: 2322 2322
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 2358 513
Size: 552 1293 4108
Fragments arranged by size:
4108 1293 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NgoMIV G'CCGG_C
Cuts at: 2320 2320
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 2578 3311 4856 1132
Size: 97 1349 733 1545 2229
Fragments arranged by size:
2229 1545 1349 733 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 2574 4852 2574
Size: 2278 3675
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5953
PfoI T'CCnGG_A
Cuts at: 2055 2055
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PspOMI G'GGCC_C
Cuts at: 2718 2718
Size: 5953
PstI C_TGCA'G
Cuts at: 830 2184 2903 3305 830
Size: 1354 719 402 3478
Fragments arranged by size:
3478 1354 719 402
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 4201 5913 4201
Size: 1712 4241
SfcI C'TryA_G
Cuts at: 826 1045 2180 2899 3301 4436 5117 5308 826
Size: 219 1135 719 402 1135 681 191 1471
Fragments arranged by size:
1471 1135 1135 719 681 402 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 2637 2745 2770 3280 1872
Size: 765 108 25 510 4545
Fragments arranged by size:
4545 765 510 108 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4057 4958 5220 820
Size: 197 926 2114 901 262 1553
Fragments arranged by size:
2114 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2285 2358 2923 3206 3392 513
Size: 552 1220 73 565 283 186 3074
Fragments arranged by size:
3074 1220 565 552 283 186 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2544 2715 3980 3997 4150 4335 4780
Size: 1255 964 171 1265 17 153 185 445
Cuts at: 4780 325
Size: 1498
Fragments arranged by size:
1498 1265 1255 964 445 185 171 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4199 5911 96
Size: 274 80 33 51 1443 2222 1712 138
Fragments arranged by size:
2222 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5913 5945 5913
Size: 32 5921
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 2635 2743 2768 3278 1870
Size: 765 108 25 510 4545
Fragments arranged by size:
4545 765 510 108 25
XmnI GAAnn'nnTTC
Cuts at: 2612 4082 2612
Size: 1470 4483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI