Restriction map for
pFN21AB9860
(Circular) (Six-base) MAPSORT of: pFN21AB9860.seq Check: 1716 from: 1 to: 5923
pFN21AB9860 5923 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3138 3986 5370 5638 1632
Size: 1506 848 1384 268 1917
Fragments arranged by size:
1917 1506 1384 848 268
AfeI AGC'GCT
Cuts at: 2429 2429
Size: 5923
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 2245 1166
Size: 1079 4844
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 5891
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaI G_GGCC'C
Cuts at: 2190 2277 2190
Size: 87 5836
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2306 3071 3232 3458 3610 5719 1493
Size: 813 765 161 226 152 2109 1697
Fragments arranged by size:
2109 1697 813 765 226 161 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2131 2221 3248 1870
Size: 73 188 90 1027 4545
Fragments arranged by size:
4545 1027 188 90 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2127 2280 3244 4699 618
Size: 325 217 627 340 153 964 1455 1842
Fragments arranged by size:
1842 1455 964 627 340 325 217 153
BanII G_rGCy'C
Cuts at: 1391 2105 2190 2277 2489 3242 1391
Size: 714 85 87 212 753 4072
Fragments arranged by size:
4072 753 714 212 87 85
BbeI G_GCGC'C
Cuts at: 2131 2284 2131
Size: 153 5770
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 928
Size: 971 138 4814
Fragments arranged by size:
4814 971 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2312 2346 4114 4148 1903
Size: 34 375 34 1768 34 3678
Fragments arranged by size:
3678 1768 375 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2442 4534 136
Size: 107 122 71 631 605 770 2092 1525
Fragments arranged by size:
2092 1525 770 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2592 5918 2592
Size: 3326 2597
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2190 2277 3984 5140 1790
Size: 296 104 87 1707 1156 2573
Fragments arranged by size:
2573 1707 1156 296 104 87
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2266 3654 4612
Size: 416 53 83 399 24 849 1388 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1388 958 849 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2124 2277 2398 3065 4583 1239
Size: 885 153 121 667 1518 2579
Fragments arranged by size:
2579 1518 885 667 153 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2119 4048 4913 5211 2119
Size: 1929 865 298 2831
Fragments arranged by size:
2831 1929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2128 2281 3730 4112
Size: 53 83 186 936 595 153 1449 382
Cuts at: 4112 4747 275
Size: 635 1451
Fragments arranged by size:
1451 1449 936 635 595 382 186 153
83 53
BsaWI w'CCGG_w
Cuts at: 2696 4356 4754 5028 5175 5586 2696
Size: 1660 398 274 147 411 3033
Fragments arranged by size:
3033 1660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 1178
Size: 30 165 30 675 30 4993
Fragments arranged by size:
4993 675 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2855 2858 2879 2882 2924 2948 2969 3005
Size: 1669 3 21 3 42 24 21 36
Cuts at: 3005 3014 3017 3029 3032 3050 3062 1186
Size: 9 3 12 3 18 12 4047
Fragments arranged by size:
4047 1669 42 36 24 21 21 18
12 12 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2079 2265 2400 2430 2683 5126 5622 2079
Size: 186 135 30 253 2443 496 2380
Fragments arranged by size:
2443 2380 496 253 186 135 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2786 3130 1163
Size: 573 1050 344 3956
Fragments arranged by size:
3956 1050 573 344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2519 3373 4134 5162 1540
Size: 275 186 518 854 761 1028 2301
Fragments arranged by size:
2301 1028 854 761 518 275 186
BsiHKAI G_wGCw'C
Cuts at: 2105 2140 2252 2507 3984 4069 5140 2105
Size: 35 112 255 1477 85 1071 2888
Fragments arranged by size:
2888 1477 1071 255 112 85 35
BsmI GAATG_Cn'
Cuts at: 2783 3434 3527 2783
Size: 651 93 5179
Fragments arranged by size:
5179 651 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2105 2140 2190 2252 2277 2489
Size: 399 296 19 35 50 62 25 212
Cuts at: 2489 2507 3242 3984 4069 5140 1391
Size: 18 735 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 735 399 296 212 85
62 50 35 25 19 18
BspEI T'CCGG_A
Cuts at: 2696 5586 2696
Size: 2890 3033
BspHI T'CATG_A
Cuts at: 2583 3812 5542 2583
Size: 1229 1730 2964
Fragments arranged by size:
2964 1730 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3278 844
Size: 2434 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2382 2670 3810 1394
Size: 429 559 288 1140 3507
Fragments arranged by size:
3507 1140 559 429 288
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2518 4567 4754 1700
Size: 110 708 2049 187 2869
Fragments arranged by size:
2869 2049 708 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 2301 2301
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2592 2690 3253 3620 4005 4022 5474 5560
Size: 620 98 563 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 620 563 385 367 346 98
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2446 2480 2513 2661 513
Size: 552 1381 34 33 148 3775
Fragments arranged by size:
3775 1381 552 148 34 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3439 4233 4253 3439
Size: 794 20 5109
Fragments arranged by size:
5109 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3156 3227 3156
Size: 71 5852
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2516 3370 4259
Size: 54 1425 50 275 18 686 854 889
Cuts at: 4259 5888 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 854 686 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2516 3370 1537
Size: 275 704 854 4090
Fragments arranged by size:
4090 854 704 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2832 2913 2934 2958 2967 3006 3021 3027
Size: 1479 81 21 24 9 39 15 6
Cuts at: 3027 3853 1353
Size: 826 3423
Fragments arranged by size:
3423 1479 826 81 39 24 21 15
9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2103 2103
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2277 2398 3065 3138 3986 4583
Size: 393 492 153 121 667 73 848 597
Cuts at: 4583 5370 5638 1239
Size: 787 268 1524
Fragments arranged by size:
1524 848 787 667 597 492 393 268
153 121 73
EcoO109I rG'GnC_Cy
Cuts at: 2187 2273 2274 2385 3040 2187
Size: 86 1 111 655 5070
Fragments arranged by size:
5070 655 111 86 1
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3165 3165
Size: 5923
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2131 2284 2431 2561 2645 5070 1678
Size: 453 153 147 130 84 2425 2531
Fragments arranged by size:
2531 2425 453 153 147 130 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373
Size: 32 135 32 2992 32 42 32 2626
Fragments arranged by size:
2992 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KasI G'GCGC_C
Cuts at: 2127 2280 2127
Size: 153 5770
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2260 5037 5221 1305
Size: 955 2777 184 2007
Fragments arranged by size:
2777 2007 955 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2245 3881 4240 4399 518
Size: 648 1079 1636 359 159 2042
Fragments arranged by size:
2042 1636 1079 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2448 2482 2495 2515 2824 3339 4016 5164
Size: 842 34 13 20 309 515 677 1148
Cuts at: 5164 5409 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 842 677 515 309 245 34
20 13
NarI GG'CG_CC
Cuts at: 2128 2281 2128
Size: 153 5770
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5371
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2554 3281 4826 1132
Size: 97 1325 727 1545 2229
Fragments arranged by size:
2229 1545 1325 727 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PfoI T'CCnGG_A
Cuts at: 2293 2387 3042 2293
Size: 94 655 5174
Fragments arranged by size:
5174 655 94
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2385 3040 2385
Size: 655 5268
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3140 3493 3140
Size: 353 5570
PspOMI G'GGCC_C
Cuts at: 2186 2273 2186
Size: 87 5836
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2579 2611 2579
Size: 32 5891
PstI C_TGCA'G
Cuts at: 830 2745 3275 830
Size: 1915 530 3478
Fragments arranged by size:
3478 1915 530
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
SacI G_AGCT'C
Cuts at: 2105 2105
Size: 5923
SacII CC_GC'GG
Cuts at: 2449 2483 2516 2449
Size: 34 33 5856
Fragments arranged by size:
5856 34 33
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2741 3271 4406 5087 5278 826
Size: 219 1696 530 1135 681 191 1471
Fragments arranged by size:
1696 1471 1135 681 530 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2442 2442
Size: 5923
SfoI GGC'GCC
Cuts at: 2129 2282 2129
Size: 153 5770
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2133 2223 3250 1872
Size: 261 90 1027 4545
Fragments arranged by size:
4545 1027 261 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2098 4027 4928 5190 820
Size: 197 926 155 1929 901 262 1553
Fragments arranged by size:
1929 1553 926 901 262 197 155
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 2554 3281 1132
Size: 1422 727 3774
Fragments arranged by size:
3774 1422 727
SrfI GCCC'GGGC
Cuts at: 2223 2223
Size: 5923
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 2678 1517
Size: 1161 4762
StyI C'CwwG_G
Cuts at: 513 1065 2192 3362 513
Size: 552 1127 1170 3074
Fragments arranged by size:
3074 1170 1127 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 2392 2588 3950 3967 4120 4305
Size: 1255 612 200 196 1362 17 153 185
Cuts at: 4305 4750 325
Size: 445 1498
Fragments arranged by size:
1498 1362 1255 612 445 200 196 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 5881 96
Size: 274 80 33 51 1443 2192 1712 138
Fragments arranged by size:
2192 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 5883 5915 2078
Size: 32 3773 32 2086
Fragments arranged by size:
3773 2086 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 2131 2221 3248 1870
Size: 261 90 1027 4545
Fragments arranged by size:
4545 1027 261 90
XmnI GAAnn'nnTTC
Cuts at: 3072 4052 3072
Size: 980 4943
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacI SacII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PvuII RsrII SanDI SapI SexAI SwaI XcmI