Restriction map for
pFN21AB9857
(Circular) (Six-base) MAPSORT of: pFN21AB9857.seq Check: 5734 from: 1 to: 5893
pFN21AB9857 5893 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2318 3703 278
Size: 53 83 186 936 782 1385 2468
Fragments arranged by size:
2468 1385 936 782 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2286 3236 1937
Size: 349 950 4594
Fragments arranged by size:
4594 950 349
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2275 3956 5340 5608 1632
Size: 643 1681 1384 268 1917
Fragments arranged by size:
1917 1681 1384 643 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2321 2468 1184
Size: 1137 147 4609
Fragments arranged by size:
4609 1137 147
AlwNI CAG_nnn'CTG
Cuts at: 2551 3636 5208 2551
Size: 1085 1572 3236
Fragments arranged by size:
3236 1572 1085
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 3202 3428 3580 5689 1493
Size: 1709 226 152 2109 1697
Fragments arranged by size:
2109 1709 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 2708 2785 3218 1870
Size: 73 307 458 77 433 4545
Fragments arranged by size:
4545 458 433 307 77 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2789 3059 3214 4669
Size: 325 217 627 518 484 270 155 1455
Cuts at: 4669 618
Size: 1842
Fragments arranged by size:
1842 1455 627 518 484 325 270 217
155
BanII G_rGCy'C
Cuts at: 1391 2085 2192 2257 3212 1391
Size: 694 107 65 955 4072
Fragments arranged by size:
4072 955 694 107 65
BbeI G_GCGC'C
Cuts at: 2793 2793
Size: 5893
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3132 928
Size: 971 1233 3689
Fragments arranged by size:
3689 1233 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4084 4118 1903
Size: 34 50 34 2063 34 3678
Fragments arranged by size:
3678 2063 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 2010 1548
Size: 462 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4504 136
Size: 107 122 71 631 605 2832 1525
Fragments arranged by size:
2832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 2519 3310 2519
Size: 791 5102
Bme1580I G_kGCm'C
Cuts at: 1790 2269 2888 3954 5110 1790
Size: 479 619 1066 1156 2573
Fragments arranged by size:
2573 1156 1066 619 479
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3624 4582 442
Size: 416 53 83 399 24 2207 958 1753
Fragments arranged by size:
2207 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2241 4553 1239
Size: 806 196 2312 2579
Fragments arranged by size:
2579 2312 806 196
Bpu10I CC'TnA_GC
Cuts at: 2634 2634
Size: 5893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2552 2599 2987 4018 4883 5181 2468
Size: 84 47 388 1031 865 298 3180
Fragments arranged by size:
3180 1031 865 388 298 84 47
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2528 4556 882
Size: 617 1029 2028 2219
Fragments arranged by size:
2219 2028 1029 617
BsaAI yAC'GTr
Cuts at: 493 1204 3684 493
Size: 711 2480 2702
Fragments arranged by size:
2702 2480 711
BsaBI GATnn'nnATC
Cuts at: 2141 5887 2141
Size: 3746 2147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2315 2390 2790 3700
Size: 53 83 186 936 782 75 400 910
Cuts at: 3700 4082 4717 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 910 782 635 400 382 186
83 75 53
BsaWI w'CCGG_w
Cuts at: 3009 4326 4724 4998 5145 5556 3009
Size: 1317 398 274 147 411 3346
Fragments arranged by size:
3346 1317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2388 2418 1178
Size: 30 165 30 985 30 4653
Fragments arranged by size:
4653 985 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 1186
Size: 967 4926
BseYI C'CCAG_C
Cuts at: 2370 2864 5096 5592 2370
Size: 494 2232 496 2671
Fragments arranged by size:
2671 2232 496 494
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2954 3954 4039 5110 2085
Size: 869 1000 85 1071 2868
Fragments arranged by size:
2868 1071 1000 869 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
BsmBI CGTCTCn'nnnn_
Cuts at: 2606 2606
Size: 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2192 2257 2269 2888 2954 3212
Size: 399 295 107 65 12 619 66 258
Cuts at: 3212 3954 4039 5110 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 399 295 258 107
85 66 65 12
BspEI T'CCGG_A
Cuts at: 3009 5556 3009
Size: 2547 3346
BspHI T'CATG_A
Cuts at: 2838 3782 5512 2838
Size: 944 1730 3219
Fragments arranged by size:
3219 1730 944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3248 844
Size: 2404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2298 4388 4562 65
Size: 1183 1050 2090 174 1396
Fragments arranged by size:
2090 1396 1183 1050 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 2280 3953 4965 2280
Size: 1673 1012 3208
Fragments arranged by size:
3208 1673 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 2047 1184
Size: 863 5030
BstYI r'GATC_y
Cuts at: 1972 2136 3223 3590 3975 3992 5444 5530 5542
Size: 164 1087 367 385 17 1452 86 12
Cuts at: 5542 5888 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1087 385 367 346 164 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3090 3090
Size: 5893
BtgI C'CryG_G
Cuts at: 513 1065 2979 513
Size: 552 1914 3427
Fragments arranged by size:
3427 1914 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2842 487
Size: 683 56 1616 3538
Fragments arranged by size:
3538 1616 683 56
BtsI GCAGTG_nn'
Cuts at: 3409 4203 4223 3409
Size: 794 20 5079
Fragments arranged by size:
5079 794 20
ClaI AT'CG_AT
Cuts at: 1554 3030 1554
Size: 1476 4417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2902 3340 4229
Size: 54 1425 50 275 18 1072 438 889
Cuts at: 4229 5858 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1072 889 438 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2643 2694 3823 1353
Size: 774 516 51 1129 3423
Fragments arranged by size:
3423 1129 774 516 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2522 4480 4854 5000 387
Size: 1064 138 933 1958 374 146 1280
Fragments arranged by size:
1958 1280 1064 933 374 146 138
EcoICRI GAG'CTC
Cuts at: 2083 2083
Size: 5893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2241 2275 3956 4553 5340 5608
Size: 393 413 196 34 1681 597 787 268
Cuts at: 5608 1239
Size: 1524
Fragments arranged by size:
1681 1524 787 597 413 393 268 196
34
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 2168 2483 2042
Size: 126 315 5452
Fragments arranged by size:
5452 315 126
EcoO109I rG'GnC_Cy
Cuts at: 2035 2098 2246 2704 2035
Size: 63 148 458 5224
Fragments arranged by size:
5224 458 148 63
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
EcoRV GAT'ATC
Cuts at: 2215 2215
Size: 5893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2612 2612
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 2793 5040 1678
Size: 1115 2247 2531
Fragments arranged by size:
2531 2247 1115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2112 2144 2151 2183 2527
Size: 32 135 32 540 32 7 32 344
Cuts at: 2527 2559 2584 2616 2820 2852 4534 4566 4608
Size: 32 25 32 204 32 1682 32 42
Cuts at: 4608 4640 1373
Size: 32 2626
Fragments arranged by size:
2626 1682 540 344 204 135 42 32
32 32 32 32 32 32 32 32
25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2782 3237 3483 669
Size: 988 281 844 455 246 3079
Fragments arranged by size:
3079 988 844 455 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KasI G'GCGC_C
Cuts at: 2789 2789
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2484 5007 5191 1305
Size: 1179 2523 184 2007
Fragments arranged by size:
2523 2007 1179 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2303 2984 3851 4210 4369 518
Size: 648 984 153 681 867 359 159 2042
Fragments arranged by size:
2042 984 867 681 648 359 159 153
MspA1I CmG'CkG
Cuts at: 1606 2366 2374 2523 3309 3986 5134 5379 1606
Size: 760 8 149 786 677 1148 245 2120
Fragments arranged by size:
2120 1148 786 760 677 245 149 8
NarI GG'CG_CC
Cuts at: 2790 2790
Size: 5893
NcoI C'CATG_G
Cuts at: 513 1065 2979 513
Size: 552 1914 3427
Fragments arranged by size:
3427 1914 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 2452 3194 3251 4796 1132
Size: 97 1223 742 57 1545 2229
Fragments arranged by size:
2229 1545 1223 742 97 57
PasI CC'CwG_GG
Cuts at: 1348 2185 1348
Size: 837 5056
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2035 2098 2246 2035
Size: 63 148 5682
Fragments arranged by size:
5682 148 63
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PstI C_TGCA'G
Cuts at: 830 2232 3245 830
Size: 1402 1013 3478
Fragments arranged by size:
3478 1402 1013
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
SacI G_AGCT'C
Cuts at: 2085 2085
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 2228 3241 4376 5057 5248 826
Size: 219 1183 1013 1135 681 191 1471
Fragments arranged by size:
1471 1183 1135 1013 681 219 191
SfoI GGC'GCC
Cuts at: 2791 2791
Size: 5893
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 2531 2578 2966 3997 4898
Size: 197 926 540 48 47 388 1031 901
Cuts at: 4898 5160 820
Size: 262 1553
Fragments arranged by size:
1553 1031 926 901 540 388 262 197
48 47
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 2452 3251 1132
Size: 1320 799 3774
Fragments arranged by size:
3774 1320 799
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 2412 2576 2997 1517
Size: 895 164 421 4413
Fragments arranged by size:
4413 895 421 164
StyI C'CwwG_G
Cuts at: 513 1065 2502 2979 3332 513
Size: 552 1437 477 353 3074
Fragments arranged by size:
3074 1437 552 477 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2868 3920 3937 4090 4275 4720 325
Size: 1255 1288 1052 17 153 185 445 1498
Fragments arranged by size:
1498 1288 1255 1052 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2019 4139 5851
Size: 274 80 33 51 1443 42 2120 1712
Cuts at: 5851 96
Size: 138
Fragments arranged by size:
2120 1712 1443 274 138 80 51 42
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2420 2459 2491 2843 2875 3109 3141 5853
Size: 32 39 32 352 32 234 32 2712
Cuts at: 5853 5885 2388
Size: 32 2396
Fragments arranged by size:
2712 2396 352 234 39 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 4022 4022
Size: 5893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2316 3701 276
Size: 53 83 186 936 782 1385 2468
Fragments arranged by size:
2468 1385 936 782 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI