Restriction map for
pFN21AB9855
(Circular) (Six-base) MAPSORT of: pFN21AB9855.seq Check: 5517 from: 1 to: 5875
pFN21AB9855 5875 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2647 2647
Size: 5875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 2079 3196 2079
Size: 1117 4758
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2536 3938 5322 5590 1632
Size: 904 1402 1384 268 1917
Fragments arranged by size:
1917 1402 1384 904 268
AfeI AGC'GCT
Cuts at: 2945 2945
Size: 5875
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 2178 2605 4774 5751 1203
Size: 975 427 2169 977 1327
Fragments arranged by size:
2169 1327 977 975 427
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 2192 1166
Size: 1026 4849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2270 1184
Size: 1086 4789
AlwNI CAG_nnn'CTG
Cuts at: 3618 5190 3618
Size: 1572 4303
ApaI G_GGCC'C
Cuts at: 2104 3105 2104
Size: 1001 4874
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2681 2846 3200 1870
Size: 73 87 651 165 354 4545
Fragments arranged by size:
4545 651 354 165 87 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2079 2695 2815 3196 4651
Size: 325 217 627 292 616 120 381 1455
Cuts at: 4651 618
Size: 1842
Fragments arranged by size:
1842 1455 627 616 381 325 292 217
120
BanII G_rGCy'C
Cuts at: 1391 2076 2104 2331 2475 3105 3194 1391
Size: 685 28 227 144 630 89 4072
Fragments arranged by size:
4072 685 630 227 144 89 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2519 928
Size: 971 620 4284
Fragments arranged by size:
4284 971 620
BbvCI CC'TCA_GC
Cuts at: 2964 3091 2964
Size: 127 5748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2303 2337 4066 4100 1903
Size: 34 50 34 282 34 1729 34 3678
Fragments arranged by size:
3678 1729 282 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 2478 2631 2769 1548
Size: 930 153 138 4654
Fragments arranged by size:
4654 930 153 138
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2685 4486 136
Size: 107 122 71 631 605 1013 1801 1525
Fragments arranged by size:
1801 1525 1013 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 2325 3292 2325
Size: 967 4908
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2104 2321 2432 2700 2955 3024 3105
Size: 299 15 217 111 268 255 69 81
Cuts at: 3105 3126 3936 5092 1790
Size: 21 810 1156 2573
Fragments arranged by size:
2573 1156 810 299 268 255 217 111
81 69 21 15
BmgBI CAC'GTC
Cuts at: 1318 2179 1318
Size: 861 5014
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3144 4535 1239
Size: 1905 1391 2579
Fragments arranged by size:
2579 1905 1391
Bpu10I CC'TnA_GC
Cuts at: 2964 3091 2964
Size: 127 5748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2186 4000 4865 5163 2186
Size: 1814 865 298 2898
Fragments arranged by size:
2898 1814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2534 4538 882
Size: 617 1035 2004 2219
Fragments arranged by size:
2219 2004 1035 617
BsaAI yAC'GTr
Cuts at: 493 1204 2041 2606 3666 493
Size: 711 837 565 1060 2702
Fragments arranged by size:
2702 1060 837 711 565
BsaBI GATnn'nnATC
Cuts at: 2450 5869 2450
Size: 3419 2456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5650
Fragments arranged by size:
5650 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 2981 1186
Size: 1252 543 4080
Fragments arranged by size:
4080 1252 543
BseYI C'CCAG_C
Cuts at: 2309 2819 5078 5574 2309
Size: 510 2259 496 2610
Fragments arranged by size:
2610 2259 510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2266 2477 2813 3019 1163
Size: 573 530 211 336 206 4019
Fragments arranged by size:
4019 573 530 336 211 206
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2587 3325 4086 5114 1540
Size: 275 186 586 738 761 1028 2301
Fragments arranged by size:
2301 1028 761 738 586 275 186
BsiHKAI G_wGCw'C
Cuts at: 3936 4021 5092 3936
Size: 85 1071 4719
Fragments arranged by size:
4719 1071 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2089 2104 2321 2331 2432 2475
Size: 399 286 13 15 217 10 101 43
Cuts at: 2475 2700 2955 3024 3105 3126 3194 3936 4021
Size: 225 255 69 81 21 68 742 85
Cuts at: 4021 5092 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 286 255 225 217
101 85 81 69 68 43 21 15
13 10
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 2475 2508 3764 5494 2475
Size: 33 1256 1730 2856
Fragments arranged by size:
2856 1730 1256 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2647 2835 3230 844
Size: 1803 188 395 3489
Fragments arranged by size:
3489 1803 395 188
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2216 4370 4544 65
Size: 1183 968 2154 174 1396
Fragments arranged by size:
2154 1396 1183 968 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4519 4706 1700
Size: 110 2709 187 2869
Fragments arranged by size:
2869 2709 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5875
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 2297 2612 1184
Size: 1113 315 4447
Fragments arranged by size:
4447 1113 315
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2194
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2928 3205 3572 3957 3974 5426 5512 5524
Size: 956 277 367 385 17 1452 86 12
Cuts at: 5524 5870 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 956 385 367 346 277 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2265 2293 2581 2721 2883 3097 3126
Size: 552 1200 28 288 140 162 214 29
Cuts at: 3126 513
Size: 3262
Fragments arranged by size:
3262 1200 552 288 214 162 140 29
28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2266 487
Size: 683 56 1040 4096
Fragments arranged by size:
4096 1040 683 56
BtsI GCAGTG_nn'
Cuts at: 2319 3391 4185 4205 2319
Size: 1072 794 20 3989
Fragments arranged by size:
3989 1072 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2199 2234 529
Size: 35 1635 35 4170
Fragments arranged by size:
4170 1635 35 35
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DraIII CAC_nnn'GTG
Cuts at: 2710 2710
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2436 2584
Size: 54 1425 50 275 18 377 229 148
Cuts at: 2584 2919 3129 3322 4211 5840 8
Size: 335 210 193 889 1629 43
Fragments arranged by size:
1629 1425 889 377 335 275 229 210
193 148 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2584 3322 1537
Size: 275 772 738 4090
Fragments arranged by size:
4090 772 738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 3805 1353
Size: 911 1541 3423
Fragments arranged by size:
3423 1541 911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2536 3144 3938 4535 5322 5590 1239
Size: 393 904 608 794 597 787 268 1524
Fragments arranged by size:
1524 904 794 787 608 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2705 2057
Size: 648 5227
EcoO109I rG'GnC_Cy
Cuts at: 2062 2100 2842 3102 2062
Size: 38 742 260 4835
Fragments arranged by size:
4835 742 260 38
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
FspI TGC'GCA
Cuts at: 3110 4381 3110
Size: 1271 4604
HaeII r_GCGC'y
Cuts at: 1678 2643 2947 5022 1678
Size: 965 304 2075 2531
Fragments arranged by size:
2531 2075 965 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3155 3187 4516 4548 4590
Size: 32 135 32 1583 32 1329 32 42
Cuts at: 4590 4622 1373
Size: 32 2626
Fragments arranged by size:
2626 1583 1329 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 2083 3200 2083
Size: 1117 4758
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4989 5173 1305
Size: 3684 184 2007
Fragments arranged by size:
3684 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2209 2438 3131 10
Size: 54 1425 343 377 229 693 2754
Fragments arranged by size:
2754 1425 693 377 343 229 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2507 2651 2657 2720 3017 3833
Size: 648 1026 315 144 6 63 297 816
Cuts at: 3833 4192 4351 518
Size: 359 159 2042
Fragments arranged by size:
2042 1026 816 648 359 315 297 159
144 63 6
MspA1I CmG'CkG
Cuts at: 1606 2095 2250 2767 3099 3291 3968 5116 5361
Size: 489 155 517 332 192 677 1148 245
Cuts at: 5361 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 517 489 332 245 192
155
NcoI C'CATG_G
Cuts at: 513 1065 2265 2721 3126 513
Size: 552 1200 456 405 3262
Fragments arranged by size:
3262 1200 552 456 405
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 2564 2656 3233 4778 1132
Size: 97 1335 92 577 1545 2229
Fragments arranged by size:
2229 1545 1335 577 97 92
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2271 2710 1108
Size: 1163 439 4273
Fragments arranged by size:
4273 1163 439
PfoI T'CCnGG_A
Cuts at: 2151 2862 2151
Size: 711 5164
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2842 2062
Size: 780 5095
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspOMI G'GGCC_C
Cuts at: 2100 3101 2100
Size: 1001 4874
PstI C_TGCA'G
Cuts at: 830 2175 3227 830
Size: 1345 1052 3478
Fragments arranged by size:
3478 1345 1052
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2250 2250
Size: 5875
SacII CC_GC'GG
Cuts at: 3100 3100
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SapI GCTCTTCn'nnn_
Cuts at: 2264 2264
Size: 5875
SbfI CC_TGCA'GG
Cuts at: 2175 3227 2175
Size: 1052 4823
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SexAI A'CCwGG_T
Cuts at: 2706 2706
Size: 5875
SfcI C'TryA_G
Cuts at: 826 1045 2171 3223 4358 5039 5230 826
Size: 219 1126 1052 1135 681 191 1471
Fragments arranged by size:
1471 1135 1126 1052 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2201 2846 3979 4880 5142 820
Size: 197 926 258 645 1133 901 262 1553
Fragments arranged by size:
1553 1133 926 901 645 262 258 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 2564 3233 1132
Size: 1432 669 3774
Fragments arranged by size:
3774 1432 669
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 2265 2721 3126 3132 3314 513
Size: 552 1200 456 405 6 182 3074
Fragments arranged by size:
3074 1200 552 456 405 182 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 2726 2833 2912 3902 3919 4072
Size: 1255 1020 126 107 79 990 17 153
Cuts at: 4072 4257 4702 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1020 990 445 185 153 126
107 79 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4121 5833 96
Size: 274 80 33 51 1443 2144 1712 138
Fragments arranged by size:
2144 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2189 2221 2424 2456 5835 5867 2189
Size: 32 203 32 3379 32 2197
Fragments arranged by size:
3379 2197 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 2393
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 2846 1943
Size: 903 4972
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI
SwaI