Restriction map for
pFN21AB9853
(Circular) (Six-base) MAPSORT of: pFN21AB9853.seq Check: 3667 from: 1 to: 5860
pFN21AB9853 5860 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2791 3203 1937
Size: 854 412 4594
Fragments arranged by size:
4594 854 412
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2867 3923 5307 5575 1632
Size: 1235 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1235 1056 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 2455 3603 5175 2455
Size: 1148 1572 3140
Fragments arranged by size:
3140 1572 1148
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2187 2571 2668 2699 2733 2934 3016 3099
Size: 694 384 97 31 34 201 82 83
Cuts at: 3099 3169 3395 3547 5656 1493
Size: 70 226 152 2109 1697
Fragments arranged by size:
2109 1697 694 384 226 201 152 97
83 82 70 34 31
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 3185 1870
Size: 73 1242 4545
Fragments arranged by size:
4545 1242 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2267 3139 3181 4636 618
Size: 325 217 627 480 872 42 1455 1842
Fragments arranged by size:
1842 1455 872 627 480 325 217 42
BanII G_rGCy'C
Cuts at: 1391 3179 1391
Size: 1788 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4889
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2261 2295 4051 4085 1903
Size: 34 324 34 1756 34 3678
Fragments arranged by size:
3678 1756 324 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 3921 5077 1790
Size: 2131 1156 2573
Fragments arranged by size:
2573 2131 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 3591 4549
Size: 416 53 83 399 24 609 1565 958
Cuts at: 4549 442
Size: 1753
Fragments arranged by size:
1753 1565 958 609 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2690 2723 4520 1239
Size: 1451 33 1797 2579
Fragments arranged by size:
2579 1797 1451 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 3045 5854 3045
Size: 2809 3051
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2960 3667 4049 4684
Size: 53 83 186 936 1427 707 382 635
Cuts at: 4684 275
Size: 1451
Fragments arranged by size:
1451 1427 936 707 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5860
BseYI C'CCAG_C
Cuts at: 5063 5559 5063
Size: 496 5364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2505 1163
Size: 573 769 4518
Fragments arranged by size:
4518 769 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3921 4006 5077 3921
Size: 85 1071 4704
Fragments arranged by size:
4704 1071 85
BsmI GAATG_Cn'
Cuts at: 2916 3371 3464 2916
Size: 455 93 5312
Fragments arranged by size:
5312 455 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2951 2951
Size: 5860
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3179 3921 4006 5077 1391
Size: 399 1389 742 85 1071 2174
Fragments arranged by size:
2174 1389 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 2839 3749 5479 2839
Size: 910 1730 3220
Fragments arranged by size:
3220 1730 910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3215 844
Size: 2371 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2027 3190 3557 3942 3959 5411 5497 5509
Size: 55 1163 367 385 17 1452 86 12
Cuts at: 5509 5855 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1163 385 367 346 86 55
17 12
BstZ17I GTA'TAC
Cuts at: 2792 2792
Size: 5860
Bsu36I CC'TnA_GG
Cuts at: 3022 3022
Size: 5860
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5308
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 3376 4170 4190 3376
Size: 794 20 5046
Fragments arranged by size:
5046 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2130 2165 529
Size: 35 1566 35 4224
Fragments arranged by size:
4224 1566 35 35
DraI TTT'AAA
Cuts at: 2827 3164 2827
Size: 337 5523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3307 4196 5825
Size: 54 1425 50 275 18 1477 889 1629
Cuts at: 5825 8
Size: 43
Fragments arranged by size:
1629 1477 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2898 3790 1353
Size: 1026 519 892 3423
Fragments arranged by size:
3423 1026 892 519
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2690 2723 2867 3923 4520 5307 5575
Size: 393 1058 33 144 1056 597 787 268
Cuts at: 5575 1239
Size: 1524
Fragments arranged by size:
1524 1058 1056 787 597 393 268 144
33
EcoRI G'AATT_C
Cuts at: 2571 3016 3169 3547 2571
Size: 445 153 378 4884
Fragments arranged by size:
4884 445 378 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2783 2783
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 5007 1678
Size: 3329 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 4501 4533 4575
Size: 32 135 32 536 32 2361 32 42
Cuts at: 4575 4607 1373
Size: 32 2626
Fragments arranged by size:
2626 2361 536 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2341 3204 3450 669
Size: 988 281 238 165 863 246 3079
Fragments arranged by size:
3079 988 863 281 246 238 165
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 2341 3450 2341
Size: 1109 4751
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 2316 3459 2316
Size: 1143 4717
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2354 4974 5158 1305
Size: 1049 2620 184 2007
Fragments arranged by size:
2620 2007 1049 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4038
Fragments arranged by size:
4038 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3818 4177 4336 518
Size: 648 2652 359 159 2042
Fragments arranged by size:
2652 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2336 2519 3276 3953 5101 5346 1606
Size: 730 183 757 677 1148 245 2120
Fragments arranged by size:
2120 1148 757 730 677 245 183
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5308
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2100 3218 4763 1132
Size: 97 871 1118 1545 2229
Fragments arranged by size:
2229 1545 1118 871 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4958
PfoI T'CCnGG_A
Cuts at: 2706 2706
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2042 3212 830
Size: 1212 1170 3478
Fragments arranged by size:
3478 1212 1170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2519 2519
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 2042 3212 2042
Size: 1170 4690
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2038 2283 2323 3028 3208 4343 5024
Size: 219 993 245 40 705 180 1135 681
Cuts at: 5024 5215 826
Size: 191 1471
Fragments arranged by size:
1471 1135 993 705 681 245 219 191
180 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 2100 3218 1132
Size: 968 1118 3774
Fragments arranged by size:
3774 1118 968
SspI AAT'ATT
Cuts at: 5 52 2251 3784 5
Size: 47 2199 1533 2081
Fragments arranged by size:
2199 2081 1533 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 3299 513
Size: 552 2234 3074
Fragments arranged by size:
3074 2234 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2360 3887 3904 4057 4242 4687 325
Size: 1255 780 1527 17 153 185 445 1498
Fragments arranged by size:
1527 1498 1255 780 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2992 2997 4106
Size: 274 80 33 51 1443 1015 5 1109
Cuts at: 4106 5818 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1109 1015 274 138 80 51
33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 2590 3196 2590
Size: 606 5254
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI