Restriction map for
pFN21AB9852
(Circular) (Six-base) MAPSORT of: pFN21AB9852.seq Check: 2898 from: 1 to: 5851
pFN21AB9852 5851 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3914 5298 5566 1632
Size: 2282 1384 268 1917
Fragments arranged by size:
2282 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 2785 4580 2785
Size: 1795 4056
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AlwNI CAG_nnn'CTG
Cuts at: 3594 5166 3594
Size: 1572 4279
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 3031 3160 3386 3538 5647 1493
Size: 1538 129 226 152 2109 1697
Fragments arranged by size:
2109 1697 1538 226 152 129
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 3176 1870
Size: 73 1233 4545
Fragments arranged by size:
4545 1233 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2261 2294 2261
Size: 33 5818
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3172 4627 618
Size: 325 217 627 1385 1455 1842
Fragments arranged by size:
1842 1455 1385 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2771 3170 1391
Size: 1380 399 4072
Fragments arranged by size:
4072 1380 399
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4042 4076 1903
Size: 34 2105 34 3678
Fragments arranged by size:
3678 2105 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2489 3743 4953 2489
Size: 1254 1210 3387
Fragments arranged by size:
3387 1254 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4462 136
Size: 107 122 71 631 605 2790 1525
Fragments arranged by size:
2790 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 2765 3268 2765
Size: 503 5348
Bme1580I G_kGCm'C
Cuts at: 1790 3912 5068 1790
Size: 2122 1156 2573
Fragments arranged by size:
2573 2122 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2941 3582 4540
Size: 416 53 83 399 24 1524 641 958
Cuts at: 4540 442
Size: 1753
Fragments arranged by size:
1753 1524 958 641 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 2085 3164 4511 1239
Size: 796 50 1079 1347 2579
Fragments arranged by size:
2579 1347 1079 796 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2566 3976 4841 5139 2327
Size: 239 1410 865 298 3039
Fragments arranged by size:
3039 1410 865 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2793 4514 882
Size: 617 1294 1721 2219
Fragments arranged by size:
2219 1721 1294 617
BsaAI yAC'GTr
Cuts at: 493 1204 3642 493
Size: 711 2438 2702
Fragments arranged by size:
2702 2438 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 4040 4675 275
Size: 53 83 186 936 2125 382 635 1451
Fragments arranged by size:
2125 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2058 2088 1178
Size: 30 165 30 655 30 4941
Fragments arranged by size:
4941 655 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2809 2841 1186
Size: 1623 32 4196
Fragments arranged by size:
4196 1623 32
BseYI C'CCAG_C
Cuts at: 2113 5054 5550 2113
Size: 2941 496 2414
Fragments arranged by size:
2941 2414 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2967 1163
Size: 573 1231 4047
Fragments arranged by size:
4047 1231 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 4062 5090 1540
Size: 275 186 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3912 3997 5068 3912
Size: 85 1071 4695
Fragments arranged by size:
4695 1071 85
BsmI GAATG_Cn'
Cuts at: 3362 3455 3362
Size: 93 5758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2771 3170 3912 3997 5068 1391
Size: 399 981 399 742 85 1071 2174
Fragments arranged by size:
2174 1071 981 742 399 399 85
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3206 844
Size: 2362 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2831 3738 1394
Size: 429 1008 907 3507
Fragments arranged by size:
3507 1008 907 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4346 4520 65
Size: 1183 3098 174 1396
Fragments arranged by size:
3098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4495 4682 1700
Size: 110 2685 187 2869
Fragments arranged by size:
2869 2685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5851
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstXI CCAn_nnnn'nTGG
Cuts at: 2360 2360
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 2572 3181 3548 3933 3950 5402 5488 5500
Size: 600 609 367 385 17 1452 86 12
Cuts at: 5500 5846 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 609 600 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2023 2023
Size: 5851
BtgI C'CryG_G
Cuts at: 513 1065 2004 2613 513
Size: 552 939 609 3751
Fragments arranged by size:
3751 939 609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5112
Fragments arranged by size:
5112 683 56
BtsI GCAGTG_nn'
Cuts at: 3367 4161 4181 3367
Size: 794 20 5037
Fragments arranged by size:
5037 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2764 2799 529
Size: 35 2200 35 3581
Fragments arranged by size:
3581 2200 35 35
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3298 4187 5816
Size: 54 1425 50 275 18 1468 889 1629
Cuts at: 5816 8
Size: 43
Fragments arranged by size:
1629 1468 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2307 2418 2489 2693 3781 1353
Size: 954 111 71 204 1088 3423
Fragments arranged by size:
3423 1088 954 204 111 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4438 4812 4958 387
Size: 1064 138 2849 374 146 1280
Fragments arranged by size:
2849 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2085 3164 3914 4511 5298 5566
Size: 393 403 50 1079 750 597 787 268
Cuts at: 5566 1239
Size: 1524
Fragments arranged by size:
1524 1079 787 750 597 403 393 268
50
EcoNI CCTnn'n_nnAGG
Cuts at: 2039 2039
Size: 5851
EcoO109I rG'GnC_Cy
Cuts at: 2239 2239
Size: 5851
EcoRI G'AATT_C
Cuts at: 3031 3160 3538 3031
Size: 129 378 5344
Fragments arranged by size:
5344 378 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 5851
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 2022 4998 1678
Size: 344 2976 2531
Fragments arranged by size:
2976 2531 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2771 2803 4492
Size: 32 135 32 563 32 604 32 1689
Cuts at: 4492 4524 4566 4598 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1689 604 563 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4965 5149 1305
Size: 3660 184 2007
Fragments arranged by size:
3660 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4029
Fragments arranged by size:
4029 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2639 2645 3809 4168 4327 518
Size: 648 1089 384 6 1164 359 159 2042
Fragments arranged by size:
2042 1164 1089 648 384 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2123 3267 3944 5092 5337 1606
Size: 517 1144 677 1148 245 2120
Fragments arranged by size:
2120 1148 1144 677 517 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4360
Fragments arranged by size:
4360 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 2380 3209 4754 1132
Size: 97 1151 829 1545 2229
Fragments arranged by size:
2229 1545 1151 829 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5851
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2239 2239
Size: 5851
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PstI C_TGCA'G
Cuts at: 830 2247 3203 830
Size: 1417 956 3478
Fragments arranged by size:
3478 1417 956
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SapI GCTCTTCn'nnn_
Cuts at: 2418 2418
Size: 5851
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 2088 4099 5811 2088
Size: 2011 1712 2128
Fragments arranged by size:
2128 2011 1712
SexAI A'CCwGG_T
Cuts at: 2179 2179
Size: 5851
SfcI C'TryA_G
Cuts at: 826 1045 2243 3199 4334 5015 5206 826
Size: 219 1198 956 1135 681 191 1471
Fragments arranged by size:
1471 1198 1135 956 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 3178 1872
Size: 1306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 2545 3955 4856 5118 820
Size: 197 926 399 203 1410 901 262 1553
Fragments arranged by size:
1553 1410 926 901 399 262 203 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2004 2655 3290 513
Size: 552 939 651 635 3074
Fragments arranged by size:
3074 939 651 635 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3878 3895 4048 4233 4678 325
Size: 1255 417 1881 17 153 185 445 1498
Fragments arranged by size:
1881 1498 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2086 4097 5809
Size: 274 80 33 51 1443 109 2011 1712
Cuts at: 5809 96
Size: 138
Fragments arranged by size:
2011 1712 1443 274 138 109 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2058 2090 5811 5843 2058
Size: 32 3721 32 2066
Fragments arranged by size:
3721 2066 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5851
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2753 2753
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 3176 1870
Size: 1306 4545
XmnI GAAnn'nnTTC
Cuts at: 2887 3980 2887
Size: 1093 4758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI