Restriction map for
pFN21AB9845
(Circular) (Six-base) MAPSORT of: pFN21AB9845.seq Check: 4922 from: 1 to: 5824
pFN21AB9845 5824 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2175 3167 1937
Size: 238 992 4594
Fragments arranged by size:
4594 992 238
Acc65I G'GTAC_C
Cuts at: 2576 3145 2576
Size: 569 5255
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3887 5271 5539 1632
Size: 2255 1384 268 1917
Fragments arranged by size:
2255 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 2271 4723 5700 1203
Size: 1068 2452 977 1327
Fragments arranged by size:
2452 1327 1068 977
AgeI A'CCGG_T
Cuts at: 2448 4655 2448
Size: 2207 3617
AhdI GACnn_n'nnGTC
Cuts at: 2934 4553 2934
Size: 1619 4205
AleI CACnn'nnGTG
Cuts at: 1166 2669 1166
Size: 1503 4321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaI G_GGCC'C
Cuts at: 2863 2863
Size: 5824
ApaLI G'TGCA_C
Cuts at: 2662 3881 5037 2662
Size: 1219 1156 3449
Fragments arranged by size:
3449 1219 1156
ApoI r'AATT_y
Cuts at: 1493 3049 3133 3359 3511 5620 1493
Size: 1556 84 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 226 152 84
AseI AT'TA_AT
Cuts at: 160 1032 2720 4378 160
Size: 872 1688 1658 1606
Fragments arranged by size:
1688 1658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 3012 3149 1870
Size: 73 1069 137 4545
Fragments arranged by size:
4545 1069 137 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2443 2476 2560 2568 2593 2601 2443
Size: 33 84 8 25 8 5666
Fragments arranged by size:
5666 84 33 25 8 8
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2576 3145 4600 618
Size: 325 217 627 789 569 1455 1842
Fragments arranged by size:
1842 1455 789 627 569 325 217
BanII G_rGCy'C
Cuts at: 1391 2863 3143 1391
Size: 1472 280 4072
Fragments arranged by size:
4072 1472 280
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 3075 928
Size: 971 328 848 3677
Fragments arranged by size:
3677 971 848 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BfrBI ATG'CAT
Cuts at: 2436 2436
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2057 4435 136
Size: 107 122 71 631 605 385 2378 1525
Fragments arranged by size:
2378 1525 631 605 385 122 107 71
BglII A'GATC_T
Cuts at: 2746 5819 2746
Size: 3073 2751
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2446 2666 2863 3885 5041 1790
Size: 656 220 197 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 656 220 197
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3555 4513 442
Size: 416 53 83 399 24 2138 958 1753
Fragments arranged by size:
2138 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2272 4484 1239
Size: 1033 2212 2579
Fragments arranged by size:
2579 2212 1033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2875 3949 4814 5112 2459
Size: 416 1074 865 298 3171
Fragments arranged by size:
3171 1074 865 416 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4487 882
Size: 617 2988 2219
Fragments arranged by size:
2988 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2305 2919 3615 493
Size: 711 1101 614 696 2702
Fragments arranged by size:
2702 1101 711 696 614
BsaBI GATnn'nnATC
Cuts at: 2513 5818 2513
Size: 3305 2519
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2448 4257 4655 4929 5076 5487 2448
Size: 1809 398 274 147 411 2785
Fragments arranged by size:
2785 1809 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5599
Fragments arranged by size:
5599 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2104 2751 2782 1186
Size: 918 647 31 4228
Fragments arranged by size:
4228 918 647 31
BseYI C'CCAG_C
Cuts at: 2881 5027 5523 2881
Size: 2146 496 3182
Fragments arranged by size:
3182 2146 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5251
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 2670 3274 4035 5063 1540
Size: 275 186 93 576 604 761 1028 2301
Fragments arranged by size:
2301 1028 761 604 576 275 186 93
BsiHKAI G_wGCw'C
Cuts at: 2666 3885 3970 5041 2666
Size: 1219 85 1071 3449
Fragments arranged by size:
3449 1219 1071 85
BsmI GAATG_Cn'
Cuts at: 2509 3335 3428 2509
Size: 826 93 4905
Fragments arranged by size:
4905 826 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2116 2116
Size: 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2446 2666 2863 3143 3885 3970 5041
Size: 399 656 220 197 280 742 85 1071
Cuts at: 5041 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 656 399 280 220 197
85
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2643 3179 844
Size: 1328 471 536 3489
Fragments arranged by size:
3489 1328 536 471
BsrBI CCG'CTC
Cuts at: 1394 1823 2256 3711 1394
Size: 429 433 1455 3507
Fragments arranged by size:
3507 1455 433 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2892 4319 4493 65
Size: 1183 1644 1427 174 1396
Fragments arranged by size:
1644 1427 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 2060 2167 2448 2487 4468 4655
Size: 110 213 37 107 281 39 1981 187
Cuts at: 4655 1700
Size: 2869
Fragments arranged by size:
2869 1981 281 213 187 110 107 39
37
BsrGI T'GTAC_A
Cuts at: 96 2268 96
Size: 2172 3652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2891 794
Size: 2097 3727
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 2355 2746 3154 3521 3906 3923 5375 5461
Size: 383 391 408 367 385 17 1452 86
Cuts at: 5461 5473 5819 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 408 391 385 383 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2670 513
Size: 552 1605 3667
Fragments arranged by size:
3667 1605 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 3340 4134 4154 2100
Size: 1240 794 20 3770
Fragments arranged by size:
3770 1240 794 20
ClaI AT'CG_AT
Cuts at: 1554 2777 1554
Size: 1223 4601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 2058 2217
Size: 54 1425 50 275 18 191 37 159
Cuts at: 2217 2289 2673 3271 4160 5789 8
Size: 72 384 598 889 1629 43
Fragments arranged by size:
1629 1425 889 598 384 275 191 159
72 54 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2570 3754 1353
Size: 1217 1184 3423
Fragments arranged by size:
3423 1217 1184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 3887 4484 5271 5539 1239
Size: 393 640 1615 597 787 268 1524
Fragments arranged by size:
1615 1524 787 640 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2859 2859
Size: 5824
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 3045 2164
Size: 881 4943
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 4971 1678
Size: 3293 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2606 2638 2920 2952 4465
Size: 32 135 32 1034 32 282 32 1513
Cuts at: 4465 4497 4539 4571 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1513 1034 282 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3027 748
Size: 2279 3545
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 2580 3149 2580
Size: 569 5255
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 2675 10
Size: 54 1425 343 387 456 3159
Fragments arranged by size:
3159 1425 456 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2264 2540 2669 3782 4141 4300
Size: 648 1059 39 276 129 1113 359 159
Cuts at: 4300 518
Size: 2042
Fragments arranged by size:
2042 1113 1059 648 359 276 159 129
39
MspA1I CmG'CkG
Cuts at: 1606 2457 3240 3917 5065 5310 1606
Size: 851 783 677 1148 245 2120
Fragments arranged by size:
2120 1148 851 783 677 245
NaeI GCC'GGC
Cuts at: 2025 2169 2489 2025
Size: 144 320 5360
Fragments arranged by size:
5360 320 144
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5272
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2023 2167 2487 2023
Size: 144 320 5360
Fragments arranged by size:
5360 320 144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NsiI A_TGCA'T
Cuts at: 2438 2438
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2275 2436 2662 3182 4727 1132
Size: 97 1046 161 226 520 1545 2229
Fragments arranged by size:
2229 1545 1046 520 226 161 97
PasI CC'CwG_GG
Cuts at: 1348 2482 1348
Size: 1134 4690
PciI A'CATG_T
Cuts at: 2271 4723 2271
Size: 2452 3372
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2859 2859
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2385 3176 830
Size: 1555 791 3478
Fragments arranged by size:
3478 1555 791
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
RsrII CG'GwC_CG
Cuts at: 2063 2063
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SexAI A'CCwGG_T
Cuts at: 2784 2784
Size: 5824
SfcI C'TryA_G
Cuts at: 826 1045 2381 2653 3172 4307 4988 5179 826
Size: 219 1336 272 519 1135 681 191 1471
Fragments arranged by size:
1471 1336 1135 681 519 272 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 2854 3928 4829 5091 820
Size: 197 926 531 380 1074 901 262 1553
Fragments arranged by size:
1553 1074 926 901 531 380 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3085 3748 5
Size: 47 3033 663 2081
Fragments arranged by size:
3033 2081 663 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2691 3263 513
Size: 552 1626 572 3074
Fragments arranged by size:
3074 1626 572 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 3851 3868 4021 4206 4651 325
Size: 1255 444 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 445 444 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 2797 4070
Size: 274 80 33 51 1443 291 529 1273
Cuts at: 4070 5782 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1273 529 291 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2357 2389 2709 2741 5784 5816 2357
Size: 32 320 32 3043 32 2365
Fragments arranged by size:
3043 2365 320 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3032 3160 3032
Size: 128 5696
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI RsrII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI