Restriction map for
pFN21AB9841
(Circular) (Six-base) MAPSORT of: pFN21AB9841.seq Check: 9730 from: 1 to: 5779
pFN21AB9841 5779 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3034 3589 278
Size: 53 83 186 936 1498 555 2468
Fragments arranged by size:
2468 1498 936 555 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2836 3842 5226 5494 1632
Size: 1204 1006 1384 268 1917
Fragments arranged by size:
1917 1384 1204 1006 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 4678 5655 1203
Size: 3475 977 1327
Fragments arranged by size:
3475 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 2462 1166
Size: 1296 4483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 2126 2815 3522 5094 2126
Size: 689 707 1572 2811
Fragments arranged by size:
2811 1572 707 689
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 2029 2837 3088 3314 3466 5575 1493
Size: 536 808 251 226 152 2109 1697
Fragments arranged by size:
2109 1697 808 536 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 3104 1870
Size: 73 1161 4545
Fragments arranged by size:
4545 1161 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 3100 4555 618
Size: 325 217 627 1184 129 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217 129
BanII G_rGCy'C
Cuts at: 1391 2363 3098 1391
Size: 972 735 4072
Fragments arranged by size:
4072 972 735
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2124 2614 928
Size: 971 225 490 4093
Fragments arranged by size:
4093 971 490 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2608 3671 4881 2608
Size: 1063 1210 3506
Fragments arranged by size:
3506 1210 1063
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2342 4390 136
Size: 107 122 71 631 605 670 2048 1525
Fragments arranged by size:
2048 1525 670 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2447 2976 3840 4996 1790
Size: 330 327 529 864 1156 2573
Fragments arranged by size:
2573 1156 864 529 330 327
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 3510 4468
Size: 416 53 83 399 24 828 1265 958
Cuts at: 4468 442
Size: 1753
Fragments arranged by size:
1753 1265 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2423 2837 4439 1239
Size: 818 366 414 1602 2579
Fragments arranged by size:
2579 1602 818 414 366
Bpu10I CC'TnA_GC
Cuts at: 2666 2666
Size: 5779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2596 3570 493
Size: 711 1392 974 2702
Fragments arranged by size:
2702 1392 974 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3031 3586 3968 4603
Size: 53 83 186 936 1498 555 382 635
Cuts at: 4603 275
Size: 1451
Fragments arranged by size:
1498 1451 936 635 555 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2160 4212 4610 4884 5031 5442 2160
Size: 2052 398 274 147 411 2497
Fragments arranged by size:
2497 2052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2557 2587 2643 2673 1178
Size: 30 165 30 1154 30 56 30 4284
Fragments arranged by size:
4284 1154 165 56 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 1186
Size: 1465 4314
BseYI C'CCAG_C
Cuts at: 2113 2409 4982 5478 2113
Size: 296 2573 496 2414
Fragments arranged by size:
2573 2414 496 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2488 2634 2770 1163
Size: 573 752 146 136 4172
Fragments arranged by size:
4172 752 573 146 136
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 3229 3990 5018 1540
Size: 275 186 60 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 3840 3925 4996 3840
Size: 85 1071 4623
Fragments arranged by size:
4623 1071 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
BsmBI CGTCTCn'nnnn_
Cuts at: 2263 2263
Size: 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2363 2447 2976 3098 3840 3925
Size: 399 330 243 84 529 122 742 85
Cuts at: 3925 4996 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 529 399 330 243 122
85 84
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3134 844
Size: 2290 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4423 4610 1700
Size: 110 2613 187 2869
Fragments arranged by size:
2869 2613 187 110
BsrGI T'GTAC_A
Cuts at: 96 2277 96
Size: 2181 3598
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 2108 3839 4851 2108
Size: 1731 1012 3036
Fragments arranged by size:
3036 1731 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 794
Size: 1508 4271
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 2893 2993 1184
Size: 1709 100 3970
Fragments arranged by size:
3970 1709 100
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 2756 2497
Size: 259 5520
BstYI r'GATC_y
Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774
Size: 1137 367 385 17 1452 86 12 346
Cuts at: 5774 1972
Size: 1977
Fragments arranged by size:
1977 1452 1137 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2967 2967
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2490 2726 3007 513
Size: 552 939 57 429 236 281 3285
Fragments arranged by size:
3285 939 552 429 281 236 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 4089 4109 3295
Size: 794 20 4965
Fragments arranged by size:
4965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DraIII CAC_nnn'GTG
Cuts at: 2302 2302
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2891 4786 809
Size: 507 1575 1895 1802
Fragments arranged by size:
1895 1802 1575 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2956 3226 4115
Size: 54 1425 50 275 18 1126 270 889
Cuts at: 4115 5744 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1126 889 275 270 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 3709 1353
Size: 743 1613 3423
Fragments arranged by size:
3423 1613 743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4366 4740 4886 387
Size: 1064 138 2777 374 146 1280
Fragments arranged by size:
2777 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2423 2836 2837 3842 4439 5226
Size: 393 425 366 413 1 1005 597 787
Cuts at: 5226 5494 1239
Size: 268 1524
Fragments arranged by size:
1524 1005 787 597 425 413 393 366
268 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2931 2931
Size: 5779
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 5779
FspI TGC'GCA
Cuts at: 2306 4285 2306
Size: 1979 3800
FspAI rTGC'GCAy
Cuts at: 2306 2306
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 4926 1678
Size: 3248 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2557 2589 3056 3088 4420
Size: 32 135 32 985 32 467 32 1332
Cuts at: 4420 4452 4494 4526 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1332 985 467 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 3123 3369 669
Size: 988 281 649 536 246 3079
Fragments arranged by size:
3079 988 649 536 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4893 5077 1305
Size: 3588 184 2007
Fragments arranged by size:
3588 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2958 10
Size: 54 1425 343 1126 2831
Fragments arranged by size:
2831 1425 1126 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 2489 2495 3737 4096 4255 518
Size: 648 1296 27 6 1242 359 159 2042
Fragments arranged by size:
2042 1296 1242 648 359 159 27 6
MspA1I CmG'CkG
Cuts at: 1606 2123 2728 3195 3872 5020 5265 1606
Size: 517 605 467 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 605 517 467 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2490 3007 513
Size: 552 939 486 517 3285
Fragments arranged by size:
3285 939 552 517 486
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2311 2555 3137 4682 1132
Size: 97 1082 244 582 1545 2229
Fragments arranged by size:
2229 1545 1082 582 244 97
PasI CC'CwG_GG
Cuts at: 1348 2828 1348
Size: 1480 4299
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PfoI T'CCnGG_A
Cuts at: 2034 2034
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2247 3131 830
Size: 1417 884 3478
Fragments arranged by size:
3478 1417 884
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2123 2123
Size: 5779
SacII CC_GC'GG
Cuts at: 2729 2729
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 2519 4027 5739 2519
Size: 1508 1712 2559
Fragments arranged by size:
2559 1712 1508
SexAI A'CCwGG_T
Cuts at: 2889 2989 2889
Size: 100 5679
SfcI C'TryA_G
Cuts at: 826 1045 2243 3127 4262 4943 5134 826
Size: 219 1198 884 1135 681 191 1471
Fragments arranged by size:
1471 1198 1135 884 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2342 2342
Size: 5779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2596 3570 493
Size: 2103 974 2702
Fragments arranged by size:
2702 2103 974
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 2311 3137 1132
Size: 1179 826 3774
Fragments arranged by size:
3774 1179 826
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2004 2490 3007 3218 513
Size: 552 939 486 517 211 3074
Fragments arranged by size:
3074 939 552 517 486 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 3806 3823 3976 4161 4606 325
Size: 1255 647 1579 17 153 185 445 1498
Fragments arranged by size:
1579 1498 1255 647 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2044 2277 2517
Size: 274 80 33 51 1443 67 233 240
Cuts at: 2517 4025 5737 96
Size: 1508 1712 138
Fragments arranged by size:
1712 1508 1443 274 240 233 138 80
67 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2643 2675 5739 5771 2643
Size: 32 3064 32 2651
Fragments arranged by size:
3064 2651 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2494 2951 2269
Size: 225 457 5097
Fragments arranged by size:
5097 457 225
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 2621 3908 2621
Size: 1287 4492
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3032 3587 276
Size: 53 83 186 936 1498 555 2468
Fragments arranged by size:
2468 1498 936 555 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacII SalI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I EcoICRI EcoO109I EcoRV
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI
SapI SfoI SrfI SwaI