Restriction map for
pFN21AB9839
(Circular) (Six-base) MAPSORT of: pFN21AB9839.seq Check: 6365 from: 1 to: 5770
pFN21AB9839 5770 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3833 5217 5485 1632
Size: 2201 1384 268 1917
Fragments arranged by size:
2201 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 2364 4669 5646 1203
Size: 1161 2305 977 1327
Fragments arranged by size:
2305 1327 1161 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 2963 3513 5085 2963
Size: 550 1572 3648
Fragments arranged by size:
3648 1572 550
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 2036 2160 2302 2633 3079 3305 3457 5566
Size: 543 124 142 331 446 226 152 2109
Cuts at: 5566 1493
Size: 1697
Fragments arranged by size:
2109 1697 543 446 331 226 152 142
124
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 3095 1870
Size: 73 1152 4545
Fragments arranged by size:
4545 1152 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2960 2993 2960
Size: 33 5737
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2610 3091 4546 618
Size: 325 217 627 823 481 1455 1842
Fragments arranged by size:
1842 1455 823 627 481 325 217
BanII G_rGCy'C
Cuts at: 1391 2814 3089 1391
Size: 1423 275 4072
Fragments arranged by size:
4072 1423 275
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 928
Size: 971 317 4482
Fragments arranged by size:
4482 971 317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 2394 2604 1548
Size: 846 210 4714
Fragments arranged by size:
4714 846 210
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 3831 4987 1790
Size: 2041 1156 2573
Fragments arranged by size:
2573 2041 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 2350 3501
Size: 416 53 83 399 24 711 222 1151
Cuts at: 3501 4459 442
Size: 958 1753
Fragments arranged by size:
1753 1151 958 711 416 399 222 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2710 4430 1239
Size: 857 614 1720 2579
Fragments arranged by size:
2579 1720 857 614
Bpu10I CC'TnA_GC
Cuts at: 3023 3023
Size: 5770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2957 3051 3895 4760 5058 2441
Size: 516 94 844 865 298 3153
Fragments arranged by size:
3153 865 844 516 298 94
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2128 4433 882
Size: 617 629 2305 2219
Fragments arranged by size:
2305 2219 629 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2949 4203 4601 4875 5022 5433 2949
Size: 1254 398 274 147 411 3286
Fragments arranged by size:
3286 1254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 1178
Size: 30 165 30 705 30 4810
Fragments arranged by size:
4810 705 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 1186
Size: 919 4851
BseYI C'CCAG_C
Cuts at: 2104 4973 5469 2104
Size: 2869 496 2405
Fragments arranged by size:
2869 2405 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2814 3831 3916 4987 2814
Size: 1017 85 1071 3597
Fragments arranged by size:
3597 1071 1017 85
BsmI GAATG_Cn'
Cuts at: 2882 3281 3374 2882
Size: 399 93 5278
Fragments arranged by size:
5278 399 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2814 3089 3831 3916 4987 1391
Size: 399 1024 275 742 85 1071 2174
Fragments arranged by size:
2174 1071 1024 742 399 275 85
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3125 844
Size: 2281 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2244 4265 4439 65
Size: 1183 996 2021 174 1396
Fragments arranged by size:
2021 1396 1183 996 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4414 4601 1700
Size: 110 2604 187 2869
Fragments arranged by size:
2869 2604 187 110
BsrGI T'GTAC_A
Cuts at: 96 2280 96
Size: 2184 3586
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstXI CCAn_nnnn'nTGG
Cuts at: 2878 2878
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765
Size: 1128 367 385 17 1452 86 12 346
Cuts at: 5765 1972
Size: 1977
Fragments arranged by size:
1977 1452 1128 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 2349 3286 4080 4100 2349
Size: 937 794 20 4019
Fragments arranged by size:
4019 937 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DraIII CAC_nnn'GTG
Cuts at: 2226 2845 2985 2226
Size: 619 140 5011
Fragments arranged by size:
5011 619 140
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 3217 4106
Size: 54 1425 50 275 18 465 922 889
Cuts at: 4106 5735 8
Size: 1629 43
Fragments arranged by size:
1629 1425 922 889 465 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2657 3700 1353
Size: 1304 1043 3423
Fragments arranged by size:
3423 1304 1043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2812 2812
Size: 5770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2710 3833 4430 5217 5485 1239
Size: 393 464 614 1123 597 787 268 1524
Fragments arranged by size:
1524 1123 787 614 597 464 393 268
EcoRI G'AATT_C
Cuts at: 2160 3079 3457 2160
Size: 919 378 4473
Fragments arranged by size:
4473 919 378
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2773 2805 4411
Size: 32 135 32 482 32 687 32 1606
Cuts at: 4411 4443 4485 4517 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1606 687 482 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 3483
Fragments arranged by size:
3483 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3728 4087 4246 518
Size: 648 2562 359 159 2042
Fragments arranged by size:
2562 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2997 3186 3863 5011 5256 1606
Size: 498 893 189 677 1148 245 2120
Fragments arranged by size:
2120 1148 893 677 498 245 189
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2368 3128 4673 1132
Size: 97 1139 760 1545 2229
Fragments arranged by size:
2229 1545 1139 760 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 2364 4669 2364
Size: 2305 3465
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2226 1108
Size: 1118 4652
PfoI T'CCnGG_A
Cuts at: 2164 2529 3054 2164
Size: 365 525 4880
Fragments arranged by size:
4880 525 365
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 3122 830
Size: 2292 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2104 2997 2104
Size: 893 4877
SacI G_AGCT'C
Cuts at: 2814 2814
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SfcI C'TryA_G
Cuts at: 826 1045 3118 4253 4934 5125 826
Size: 219 2073 1135 681 191 1471
Fragments arranged by size:
2073 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2456 2972 3066 3874 4775 5037
Size: 197 926 513 516 94 808 901 262
Cuts at: 5037 820
Size: 1553
Fragments arranged by size:
1553 926 901 808 516 513 262 197
94
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 3209 513
Size: 552 939 1205 3074
Fragments arranged by size:
3074 1205 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2617 3797 3814 3967 4152 4597 325
Size: 1255 1037 1180 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1180 1037 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 2523 2976
Size: 274 80 33 51 1443 303 243 453
Cuts at: 2976 4016 5728 96
Size: 1040 1712 138
Fragments arranged by size:
1712 1443 1040 453 303 274 243 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2108 2140 5730 5762 2108
Size: 32 3590 32 2116
Fragments arranged by size:
3590 2116 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2315 2011
Size: 304 5466
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI