Restriction map for
pFN21AB9831
(Circular) (Six-base) MAPSORT of: pFN21AB9831.seq Check: 3569 from: 1 to: 5731
pFN21AB9831 5731 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2229 3074 1937
Size: 292 845 4594
Fragments arranged by size:
4594 845 292
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 3003 3794 5178 5446 1632
Size: 1065 306 791 1384 268 1917
Fragments arranged by size:
1917 1384 1065 791 306 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 2984 1166
Size: 1818 3913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 2024
Size: 32 5699
AlwNI CAG_nnn'CTG
Cuts at: 3474 5046 3474
Size: 1572 4159
ApaLI G'TGCA_C
Cuts at: 2707 3788 4944 2707
Size: 1081 1156 3494
Fragments arranged by size:
3494 1156 1081
ApoI r'AATT_y
Cuts at: 1493 2147 2264 2801 3040 3266 3418 5527 1493
Size: 654 117 537 239 226 152 2109 1697
Fragments arranged by size:
2109 1697 654 537 239 226 152 117
AseI AT'TA_AT
Cuts at: 160 1032 2817 4285 160
Size: 872 1785 1468 1606
Fragments arranged by size:
1785 1606 1468 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2958 3056 1870
Size: 73 144 871 98 4545
Fragments arranged by size:
4545 871 144 98 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3052 4507 618
Size: 325 217 627 1265 1455 1842
Fragments arranged by size:
1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3050 1391
Size: 1659 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2590 928
Size: 971 691 4069
Fragments arranged by size:
4069 971 691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2247 2965 3623 4833 2247
Size: 718 658 1210 3145
Fragments arranged by size:
3145 1210 718 658
BclI T'GATC_A
Cuts at: 1548 2205 1548
Size: 657 5074
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2163 5726 2163
Size: 3563 2168
BlpI GC'TnA_GC
Cuts at: 2778 3148 2778
Size: 370 5361
Bme1580I G_kGCm'C
Cuts at: 1790 2490 2711 2745 3792 4948 1790
Size: 700 221 34 1047 1156 2573
Fragments arranged by size:
2573 1156 1047 700 221 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4391 1239
Size: 3152 2579
Bpu10I CC'TnA_GC
Cuts at: 2382 2503 2849 2382
Size: 121 346 5264
Fragments arranged by size:
5264 346 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2110 2752 3522 493
Size: 711 906 642 770 2702
Fragments arranged by size:
2702 906 770 711 642
BsaBI GATnn'nnATC
Cuts at: 2162 2826 5725 2162
Size: 664 2899 2168
Fragments arranged by size:
2899 2168 664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2331 3538 3920 4555
Size: 53 83 186 936 798 1207 382 635
Cuts at: 4555 275
Size: 1451
Fragments arranged by size:
1451 1207 936 798 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2011 1186
Size: 825 4906
BseYI C'CCAG_C
Cuts at: 2782 4934 5430 2782
Size: 2152 496 3083
Fragments arranged by size:
3083 2152 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2678 2777 1163
Size: 573 942 99 4117
Fragments arranged by size:
4117 942 573 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2711 3792 3877 4948 2088
Size: 623 1081 85 1071 2871
Fragments arranged by size:
2871 1081 1071 623 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2490 2711 2745 3050 3792 3877
Size: 399 298 402 221 34 305 742 85
Cuts at: 3877 4948 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 305 298 221
85 34
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3086 844
Size: 2242 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2094 4226 4400 65
Size: 1183 846 2132 174 1396
Fragments arranged by size:
2132 1396 1183 846 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2653 4375 4562 1700
Size: 110 843 1722 187 2869
Fragments arranged by size:
2869 1722 843 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2656 794
Size: 1862 3869
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 2833 1184
Size: 1649 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 3011 3011
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 2163 3006 3061 3428 3813 3830 5282 5368
Size: 191 843 55 367 385 17 1452 86
Cuts at: 5368 5380 5726 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 843 385 367 346 191 86
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2184 2967 513
Size: 552 939 180 783 3277
Fragments arranged by size:
3277 939 783 552 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 3247 4041 4061 3247
Size: 794 20 4917
Fragments arranged by size:
4917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DraIII CAC_nnn'GTG
Cuts at: 2752 2755 2752
Size: 3 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2433 3178 4067
Size: 54 1425 50 275 18 603 745 889
Cuts at: 4067 5696 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 745 603 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2204 2618 3661 1353
Size: 707 144 414 1043 3423
Fragments arranged by size:
3423 1043 707 414 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2204 4318 4692 4838 387
Size: 1064 138 615 2114 374 146 1280
Fragments arranged by size:
2114 1280 1064 615 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2697 3003 3794 4391 5178 5446 1239
Size: 393 1065 306 791 597 787 268 1524
Fragments arranged by size:
1524 1065 791 787 597 393 306 268
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 2939 4237 2939
Size: 1298 4433
HaeII r_GCGC'y
Cuts at: 1678 2689 2696 4878 1678
Size: 1011 7 2182 2531
Fragments arranged by size:
2531 2182 1011 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2037 2069 4372 4404 4446
Size: 32 135 32 465 32 2303 32 42
Cuts at: 4446 4478 1373
Size: 32 2626
Fragments arranged by size:
2626 2303 465 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4845 5029 1305
Size: 3540 184 2007
Fragments arranged by size:
3540 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 10
Size: 54 1425 343 603 3306
Fragments arranged by size:
3306 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2429 2984 3689 4048 4207 518
Size: 648 915 348 555 705 359 159 2042
Fragments arranged by size:
2042 915 705 648 555 359 348 159
MspA1I CmG'CkG
Cuts at: 1606 2761 3147 3824 4972 5217 1606
Size: 1155 386 677 1148 245 2120
Fragments arranged by size:
2120 1155 1148 677 386 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2184 513
Size: 552 939 180 4060
Fragments arranged by size:
4060 939 552 180
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2197 2938 3089 4634 1132
Size: 97 968 741 151 1545 2229
Fragments arranged by size:
2229 1545 968 741 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2752 1108
Size: 1644 4087
PfoI T'CCnGG_A
Cuts at: 2466 2790 2466
Size: 324 5407
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PmlI CAC'GTG
Cuts at: 2752 2752
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 2343 3301 2343
Size: 958 4773
PstI C_TGCA'G
Cuts at: 830 3083 830
Size: 2253 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2761 2761
Size: 5731
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SapI GCTCTTCn'nnn_
Cuts at: 2204 2204
Size: 5731
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SexAI A'CCwGG_T
Cuts at: 2622 2622
Size: 5731
SfcI C'TryA_G
Cuts at: 826 1045 2929 3079 4214 4895 5086 826
Size: 219 1884 150 1135 681 191 1471
Fragments arranged by size:
1884 1471 1135 681 219 191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2960 3058 1872
Size: 1088 98 4545
Fragments arranged by size:
4545 1088 98
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3835 4736 4998 820
Size: 197 926 1892 901 262 1553
Fragments arranged by size:
1892 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 2938 3089 1132
Size: 1806 151 3774
Fragments arranged by size:
3774 1806 151
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 2004 2184 2418 3170 513
Size: 552 939 180 234 752 3074
Fragments arranged by size:
3074 939 752 552 234 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2419 3758 3775 3928 4113 4558 325
Size: 1255 839 1339 17 153 185 445 1498
Fragments arranged by size:
1498 1339 1255 839 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3977 5689 96
Size: 274 80 33 51 1443 2000 1712 138
Fragments arranged by size:
2000 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 2958 3056 1870
Size: 1088 98 4545
Fragments arranged by size:
4545 1088 98
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI