Restriction map for
pFN21AB9824
(Circular) (Six-base) MAPSORT of: pFN21AB9824.seq Check: 4336 from: 1 to: 5650
pFN21AB9824 5650 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3713 5097 5365 1632
Size: 2081 1384 268 1917
Fragments arranged by size:
2081 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2492 820
Size: 197 1475 3978
Fragments arranged by size:
3978 1475 197
AflIII A'CryG_T
Cuts at: 1203 2119 4549 5526 1203
Size: 916 2430 977 1327
Fragments arranged by size:
2430 1327 977 916
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 2407 3393 4965 2407
Size: 986 1572 3092
Fragments arranged by size:
3092 1572 986
ApaLI G'TGCA_C
Cuts at: 2569 3707 4863 2569
Size: 1138 1156 3356
Fragments arranged by size:
3356 1156 1138
ApoI r'AATT_y
Cuts at: 1493 2959 3185 3337 5446 1493
Size: 1466 226 152 2109 1697
Fragments arranged by size:
2109 1697 1466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
AvrII C'CTAG_G
Cuts at: 2279 2927 2279
Size: 648 5002
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2088 2205 2320 2478 2969 1391
Size: 629 68 117 115 158 491 4072
Fragments arranged by size:
4072 629 491 158 117 115 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2281 4261
Size: 107 122 71 631 605 337 272 1980
Cuts at: 4261 136
Size: 1525
Fragments arranged by size:
1980 1525 631 605 337 272 122 107
71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2573 3711 4867 1790
Size: 783 1138 1156 2573
Fragments arranged by size:
2573 1156 1138 783
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2120 2120
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 4310 1239
Size: 1031 2040 2579
Fragments arranged by size:
2579 2040 1031
Bpu10I CC'TnA_GC
Cuts at: 2262 2350 2262
Size: 88 5562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2462 3775 4640 4938 2189
Size: 273 1313 865 298 2901
Fragments arranged by size:
2901 1313 865 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2681 4313 882
Size: 617 1182 1632 2219
Fragments arranged by size:
2219 1632 1182 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 2100 3457 3839
Size: 53 83 186 936 521 46 1357 382
Cuts at: 3839 4474 275
Size: 635 1451
Fragments arranged by size:
1451 1357 936 635 521 382 186 83
53 46
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2205 2383 2416 1186
Size: 1019 178 33 4420
Fragments arranged by size:
4420 1019 178 33
BseYI C'CCAG_C
Cuts at: 2109 4853 5349 2109
Size: 2744 496 2410
Fragments arranged by size:
2744 2410 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2295 2507 1163
Size: 573 559 212 4306
Fragments arranged by size:
4306 573 559 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2020 2268 2478 2573 3711 3796 4867 2020
Size: 248 210 95 1138 85 1071 2803
Fragments arranged by size:
2803 1138 1071 248 210 95 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
BsmBI CGTCTCn'nnnn_
Cuts at: 2091 2091
Size: 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2088 2205 2268 2320 2478 2573
Size: 399 230 68 117 63 52 158 95
Cuts at: 2573 2969 3711 3796 4867 1391
Size: 396 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 230 158 117
95 85 68 63 52
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2104 2287 3005 844
Size: 1260 183 718 3489
Fragments arranged by size:
3489 1260 718 183
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4145 4319 65
Size: 1183 2897 174 1396
Fragments arranged by size:
2897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 2753 96
Size: 2657 2993
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 2240 2313 3710 4722 2240
Size: 73 1397 1012 3168
Fragments arranged by size:
3168 1397 1012 73
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2044 794
Size: 1250 4400
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2598 2598
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2330 2601 2980 3347 3732 3749 5201 5287
Size: 358 271 379 367 385 17 1452 86
Cuts at: 5287 5299 5645 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 379 367 358 346 271
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2251 2769 513
Size: 552 939 247 518 3394
Fragments arranged by size:
3394 939 552 518 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 2621 3166 3960 3980 2621
Size: 545 794 20 4291
Fragments arranged by size:
4291 794 545 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2253 4657 809
Size: 507 937 2404 1802
Fragments arranged by size:
2404 1802 937 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3097 3986 5615
Size: 54 1425 50 275 18 1267 889 1629
Cuts at: 5615 8
Size: 43
Fragments arranged by size:
1629 1425 1267 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2684 3580 1353
Size: 1331 896 3423
Fragments arranged by size:
3423 1331 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2018 2476 2018
Size: 458 5192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 3713 4310 5097 5365 1239
Size: 393 638 1443 597 787 268 1524
Fragments arranged by size:
1524 1443 787 638 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2578 2578
Size: 5650
EcoO109I rG'GnC_Cy
Cuts at: 2177 2177
Size: 5650
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2187 2219 4291 4323 4365
Size: 32 135 32 615 32 2072 32 42
Cuts at: 4365 4397 1373
Size: 32 2626
Fragments arranged by size:
2626 2072 615 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2863 2994 3240 669
Size: 988 281 925 131 246 3079
Fragments arranged by size:
3079 988 925 281 246 131
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 2863 3240 2863
Size: 377 5273
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4764 4948 1305
Size: 3459 184 2007
Fragments arranged by size:
3459 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3828
Fragments arranged by size:
3828 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3608 3967 4126 518
Size: 648 2442 359 159 2042
Fragments arranged by size:
2442 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2880 3066 3743 4891 5136 1606
Size: 1274 186 677 1148 245 2120
Fragments arranged by size:
2120 1274 1148 677 245 186
NcoI C'CATG_G
Cuts at: 513 1065 2004 2769 513
Size: 552 939 765 3394
Fragments arranged by size:
3394 939 765 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2123 3008 4553 1132
Size: 97 894 885 1545 2229
Fragments arranged by size:
2229 1545 894 885 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 2119 4549 2119
Size: 2430 3220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2198 1108
Size: 1090 4560
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2052 3002 830
Size: 1222 950 3478
Fragments arranged by size:
3478 1222 950
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SacI G_AGCT'C
Cuts at: 2020 2478 2020
Size: 458 5192
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2048 2530 2998 4133 4814 5005 826
Size: 219 1003 482 468 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 681 482 468 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 2477 2492 3754 4655 4917
Size: 197 926 261 273 15 1262 901 262
Cuts at: 4917 820
Size: 1553
Fragments arranged by size:
1553 1262 926 901 273 262 261 197
15
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2584 2869 1517
Size: 1067 285 4298
Fragments arranged by size:
4298 1067 285
StyI C'CwwG_G
Cuts at: 513 1065 2004 2279 2579 2769 2820 2927 3089
Size: 552 939 275 300 190 51 107 162
Cuts at: 3089 513
Size: 3074
Fragments arranged by size:
3074 939 552 300 275 190 162 107
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 2762 3677 3694 3847 4032 4477
Size: 1255 543 639 915 17 153 185 445
Cuts at: 4477 325
Size: 1498
Fragments arranged by size:
1498 1255 915 639 543 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2753 3896
Size: 274 80 33 51 1443 189 587 1143
Cuts at: 3896 5608 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1143 587 274 189 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI