Restriction map for
pFN21AB9820
(Circular) (Six-base) MAPSORT of: pFN21AB9820.seq Check: 4195 from: 1 to: 5638
pFN21AB9820 5638 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3701 5085 5353 1632
Size: 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2774 820
Size: 197 1757 3684
Fragments arranged by size:
3684 1757 197
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 2359 4367 2359
Size: 2008 3630
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2157 1184
Size: 973 4665
AlwNI CAG_nnn'CTG
Cuts at: 3381 4953 3381
Size: 1572 4066
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2030 2947 3173 3325 5434 1493
Size: 537 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 537 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2761 2832 2959 4414 618
Size: 325 217 627 974 71 127 1455 1842
Fragments arranged by size:
1842 1455 974 627 325 217 127 71
BanII G_rGCy'C
Cuts at: 1391 2466 2957 1391
Size: 1075 491 4072
Fragments arranged by size:
4072 1075 491
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BbvCI CC'TCA_GC
Cuts at: 2337 2481 2337
Size: 144 5494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2450 3530 4740 2450
Size: 1080 1210 3348
Fragments arranged by size:
3348 1210 1080
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2274 4249 136
Size: 107 122 71 631 605 602 1975 1525
Fragments arranged by size:
1975 1525 631 605 602 122 107 71
BglII A'GATC_T
Cuts at: 2655 5633 2655
Size: 2978 2660
BlpI GC'TnA_GC
Cuts at: 2130 3055 2130
Size: 925 4713
Bme1580I G_kGCm'C
Cuts at: 1790 2270 2835 3699 4855 1790
Size: 480 565 864 1156 2573
Fragments arranged by size:
2573 1156 864 565 480
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 2193 2292
Size: 416 53 83 399 24 666 110 99
Cuts at: 2292 2445 3369 4327 442
Size: 153 924 958 1753
Fragments arranged by size:
1753 958 924 666 416 399 153 110
99 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2392 2489 4298 1239
Size: 1153 97 1809 2579
Fragments arranged by size:
2579 1809 1153 97
Bpu10I CC'TnA_GC
Cuts at: 2337 2481 2863 2337
Size: 144 382 5112
Fragments arranged by size:
5112 382 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2188 4301 882
Size: 617 689 2113 2219
Fragments arranged by size:
2219 2113 689 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2701 4071 4469 4743 4890 5301 2701
Size: 1370 398 274 147 411 3038
Fragments arranged by size:
3038 1370 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2185 2215 2483 2513 1178
Size: 30 165 30 782 30 268 30 4303
Fragments arranged by size:
4303 782 268 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2206 2489 2864 1186
Size: 1020 283 375 3960
Fragments arranged by size:
3960 1020 375 283
BseYI C'CCAG_C
Cuts at: 4841 5337 4841
Size: 496 5142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2669 1163
Size: 573 933 4132
Fragments arranged by size:
4132 933 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2123 2466 2720 3699 3784 4855 2123
Size: 343 254 979 85 1071 2906
Fragments arranged by size:
2906 1071 979 343 254 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2270 2466 2720 2835 2957 3699
Size: 399 333 147 196 254 115 122 742
Cuts at: 3699 3784 4855 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 333 254 196 147
122 115 85
BspEI T'CCGG_A
Cuts at: 2701 5301 2701
Size: 2600 3038
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 2856 3698 4710 2856
Size: 842 1012 3784
Fragments arranged by size:
3784 1012 842
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2311 2655 2968 3335 3720 3737 5189 5275
Size: 339 344 313 367 385 17 1452 86
Cuts at: 5275 5287 5633 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 344 339 313
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2047 513
Size: 552 939 43 4104
Fragments arranged by size:
4104 939 552 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 3085 3974
Size: 54 1425 50 275 18 220 1035 889
Cuts at: 3974 5603 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1035 889 275 220 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 2466 2708 3568 1353
Size: 830 283 242 860 3423
Fragments arranged by size:
3423 860 830 283 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2464 2464
Size: 5638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2392 2489 3701 4298 5085 5353 1239
Size: 393 760 97 1212 597 787 268 1524
Fragments arranged by size:
1524 1212 787 760 597 393 268 97
EcoNI CCTnn'n_nnAGG
Cuts at: 2575 2575
Size: 5638
EcoO109I rG'GnC_Cy
Cuts at: 2056 2287 2580 2056
Size: 231 293 5114
Fragments arranged by size:
5114 293 231
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 2185 2217 2345
Size: 32 135 32 533 32 48 32 128
Cuts at: 2345 2377 2483 2515 4279 4311 4353 4385 1373
Size: 32 106 32 1764 32 42 32 2626
Fragments arranged by size:
2626 1764 533 135 128 106 48 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 2982 3228 669
Size: 988 281 981 63 246 3079
Fragments arranged by size:
3079 988 981 281 246 63
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 2590 3237 2590
Size: 647 4991
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2137 4752 4936 1305
Size: 832 2615 184 2007
Fragments arranged by size:
2615 2007 832 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2052 10
Size: 54 1425 343 220 3596
Fragments arranged by size:
3596 1425 343 220 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3596 3955 4114 518
Size: 648 2430 359 159 2042
Fragments arranged by size:
2430 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2047 513
Size: 552 939 43 4104
Fragments arranged by size:
4104 939 552 43
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2060 1348
Size: 712 4926
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2238 1108
Size: 1130 4508
PfoI T'CCnGG_A
Cuts at: 2742 2742
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2056
Size: 5638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2990 830
Size: 2160 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SacI G_AGCT'C
Cuts at: 2466 2466
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SapI GCTCTTCn'nnn_
Cuts at: 2708 2708
Size: 5638
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2986 4121 4802 4993 826
Size: 219 1941 1135 681 191 1471
Fragments arranged by size:
1941 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2774 3742 4643 4905 820
Size: 197 926 831 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 831 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 2047 2272 3077 513
Size: 552 939 43 225 805 3074
Fragments arranged by size:
3074 939 805 552 225 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 3665 3682 3835 4020 4465 325
Size: 1255 656 1429 17 153 185 445 1498
Fragments arranged by size:
1498 1429 1255 656 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3884 5596 96
Size: 274 80 33 51 1443 1907 1712 138
Fragments arranged by size:
1907 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 2199 1316
Size: 883 4755
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI