Restriction map for
pFN21AB9819
(Circular) (Six-base) MAPSORT of: pFN21AB9819.seq Check: 8819 from: 1 to: 5635
pFN21AB9819 5635 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2445 2829 2445
Size: 384 5251
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3698 5082 5350 1632
Size: 2066 1384 268 1917
Fragments arranged by size:
2066 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 2932 4534 5511 1203
Size: 1729 1602 977 1327
Fragments arranged by size:
1729 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 2571 1166
Size: 1405 4230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2589 1184
Size: 1405 4230
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaI G_GGCC'C
Cuts at: 2094 2094
Size: 5635
ApaLI G'TGCA_C
Cuts at: 2432 3692 4848 2432
Size: 1260 1156 3219
Fragments arranged by size:
3219 1260 1156
ApoI r'AATT_y
Cuts at: 1493 2944 3170 3322 5431 1493
Size: 1451 226 152 2109 1697
Fragments arranged by size:
2109 1697 1451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2330 2729 2879 2960 1870
Size: 73 387 399 150 81 4545
Fragments arranged by size:
4545 399 387 150 81 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2573 2625 2883 2956 4411
Size: 325 217 627 786 52 258 73 1455
Cuts at: 4411 618
Size: 1842
Fragments arranged by size:
1842 1455 786 627 325 258 217 73
52
BanII G_rGCy'C
Cuts at: 1391 2094 2306 2811 2954 1391
Size: 703 212 505 143 4072
Fragments arranged by size:
4072 703 505 212 143
BbeI G_GCGC'C
Cuts at: 2887 2887
Size: 5635
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 2754 1548
Size: 1206 4429
BfrBI ATG'CAT
Cuts at: 2277 2277
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2161 2493 2634
Size: 107 122 71 631 605 489 332 141
Cuts at: 2634 2689 2881 4246 136
Size: 55 192 1365 1525
Fragments arranged by size:
1525 1365 631 605 489 332 192 141
122 107 71 55
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 2531 2591 3052 2531
Size: 60 461 5114
Fragments arranged by size:
5114 461 60
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2436 3696 4852 1790
Size: 304 342 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 342 304
BmgBI CAC'GTC
Cuts at: 1318 2137 1318
Size: 819 4816
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2410 3366 4324
Size: 416 53 83 399 24 993 956 958
Cuts at: 4324 442
Size: 1753
Fragments arranged by size:
1753 993 958 956 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2521 4295 1239
Size: 1229 53 1774 2579
Fragments arranged by size:
2579 1774 1229 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2110 4298 882
Size: 617 611 2188 2219
Fragments arranged by size:
2219 2188 617 611
BsaAI yAC'GTr
Cuts at: 493 1204 2569 3426 493
Size: 711 1365 857 2702
Fragments arranged by size:
2702 1365 857 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2475 2884 3442 3824
Size: 53 83 186 936 942 409 558 382
Cuts at: 3824 4459 275
Size: 635 1451
Fragments arranged by size:
1451 942 936 635 558 409 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 2363 2393 2860
Size: 30 165 30 699 30 231 30 467
Cuts at: 2860 2890 2910 2940 1178
Size: 30 20 30 3873
Fragments arranged by size:
3873 699 467 231 165 30 30 30
30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2855 2921 2924 1186
Size: 1669 66 3 3897
Fragments arranged by size:
3897 1669 66 3
BseYI C'CCAG_C
Cuts at: 2211 2897 4838 5334 2211
Size: 686 1941 496 2512
Fragments arranged by size:
2512 1941 686 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2423 2807 1163
Size: 573 486 201 384 3991
Fragments arranged by size:
3991 573 486 384 201
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2497 2796 3085 3846 4874
Size: 275 186 60 436 299 289 761 1028
Cuts at: 4874 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 436 299 289 275 186
60
BsiHKAI G_wGCw'C
Cuts at: 2436 2811 3696 3781 4852 2436
Size: 375 885 85 1071 3219
Fragments arranged by size:
3219 1071 885 375 85
BsmI GAATG_Cn'
Cuts at: 2255 2279 3146 3239 2255
Size: 24 867 93 4651
Fragments arranged by size:
4651 867 93 24
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2306 2436 2811 2954 3696 3781
Size: 399 304 212 130 375 143 742 85
Cuts at: 3781 4852 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 304 212 143
130 85
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 2445 2829 2990 844
Size: 1502 99 384 161 3489
Fragments arranged by size:
3489 1502 384 161 99
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2442 2688 4279 4466 1700
Size: 110 632 246 1591 187 2869
Fragments arranged by size:
2869 1591 632 246 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 2788 1925
Size: 863 4772
BssSI C'ACGA_G
Cuts at: 2240 3695 4707 2240
Size: 1455 1012 3168
Fragments arranged by size:
3168 1455 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 2545
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630
Size: 993 367 385 17 1452 86 12 346
Cuts at: 5630 1972
Size: 1977
Fragments arranged by size:
1977 1452 993 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2157 2157
Size: 5635
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2231 2544 513
Size: 552 939 51 176 313 3604
Fragments arranged by size:
3604 939 552 313 176 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 2931 3151 3945 3965 2931
Size: 220 794 20 4601
Fragments arranged by size:
4601 794 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DraIII CAC_nnn'GTG
Cuts at: 2245 2245
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2494 2793
Size: 54 1425 50 275 18 228 436 299
Cuts at: 2793 3082 3971 5600 8
Size: 289 889 1629 43
Fragments arranged by size:
1629 1425 889 436 299 289 275 228
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 2494 2793 3082 1537
Size: 275 246 436 299 289 4090
Fragments arranged by size:
4090 436 299 289 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2282 3565 1353
Size: 929 1283 3423
Fragments arranged by size:
3423 1283 929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2846 4222 4596 4742 387
Size: 1064 138 1257 1376 374 146 1280
Fragments arranged by size:
1376 1280 1257 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2809 2809
Size: 5635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 2521 3698 4295 5082 5350 1239
Size: 393 836 53 1177 597 787 268 1524
Fragments arranged by size:
1524 1177 836 787 597 393 268 53
EcoO109I rG'GnC_Cy
Cuts at: 2091 2749 2091
Size: 658 4977
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FspI TGC'GCA
Cuts at: 2552 2669 4141 2552
Size: 117 1472 4046
Fragments arranged by size:
4046 1472 117
HaeII r_GCGC'y
Cuts at: 1678 2315 2544 2887 4782 1678
Size: 637 229 343 1895 2531
Fragments arranged by size:
2531 1895 637 343 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 4276 4308 4350
Size: 32 135 32 791 32 1881 32 42
Cuts at: 4350 4382 1373
Size: 32 2626
Fragments arranged by size:
2626 1881 791 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 2979 3225 669
Size: 988 281 568 473 246 3079
Fragments arranged by size:
3079 988 568 473 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KasI G'GCGC_C
Cuts at: 2883 2883
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4749 4933 1305
Size: 3444 184 2007
Fragments arranged by size:
3444 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3813
Fragments arranged by size:
3813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2571 3593 3952 4111 518
Size: 648 1405 1022 359 159 2042
Fragments arranged by size:
2042 1405 1022 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2233 2704 3051 3728 4876 5121 1606
Size: 627 471 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 627 471 347 245
NarI GG'CG_CC
Cuts at: 2884 2884
Size: 5635
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 2494 3082 2494
Size: 588 5047
NsiI A_TGCA'T
Cuts at: 2279 2279
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PmlI CAC'GTG
Cuts at: 2569 2569
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2749 2749
Size: 5635
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PspOMI G'GGCC_C
Cuts at: 2090 2090
Size: 5635
PspXI vC'TCGA_Gb
Cuts at: 2729 2729
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2697 2805 2987 830
Size: 1867 108 182 3478
Fragments arranged by size:
3478 1867 182 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2704 2704
Size: 5635
SacI G_AGCT'C
Cuts at: 2811 2811
Size: 5635
SacII CC_GC'GG
Cuts at: 2234 2234
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SapI GCTCTTCn'nnn_
Cuts at: 2282 2282
Size: 5635
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SexAI A'CCwGG_T
Cuts at: 2013 2013
Size: 5635
SfcI C'TryA_G
Cuts at: 826 1045 2693 2801 2983 4118 4799 4990 826
Size: 219 1648 108 182 1135 681 191 1471
Fragments arranged by size:
1648 1471 1135 681 219 191 182 108
SfiI GGCCn_nnn'nGGCC
Cuts at: 2493 2493
Size: 5635
SfoI GGC'GCC
Cuts at: 2885 2885
Size: 5635
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2881 2962 1872
Size: 1009 81 4545
Fragments arranged by size:
4545 1009 81
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 3739 4640 4902 820
Size: 197 926 786 1010 901 262 1553
Fragments arranged by size:
1553 1010 926 901 786 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2004 2577 3074 513
Size: 552 939 573 497 3074
Fragments arranged by size:
3074 939 573 552 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2561 3662 3679 3832 4017 4462 325
Size: 1255 981 1101 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1101 981 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2910 2942 5595 5627 2910
Size: 32 2653 32 2918
Fragments arranged by size:
2918 2653 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2743 2971 2743
Size: 228 5407
XhoI C'TCGA_G
Cuts at: 1943 2729 1943
Size: 786 4849
XmaI C'CCGG_G
Cuts at: 1870 2879 2960 1870
Size: 1009 81 4545
Fragments arranged by size:
4545 1009 81
XmnI GAAnn'nnTTC
Cuts at: 2291 3764 2291
Size: 1473 4162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PfoI PsrI RsrII SanDI
SrfI SwaI XcmI