Restriction map for
pFN21AB9818
(Circular) (Six-base) MAPSORT of: pFN21AB9818.seq Check: 5600 from: 1 to: 5632
pFN21AB9818 5632 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2531 2752 2531
Size: 221 5411
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3695 5079 5347 1632
Size: 2063 1384 268 1917
Fragments arranged by size:
2063 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2279 2311 2279
Size: 32 5600
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaI G_GGCC'C
Cuts at: 2815 2815
Size: 5632
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
AvrII C'CTAG_G
Cuts at: 2527 2527
Size: 5632
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2140 2764 2953 4408
Size: 325 217 627 339 14 624 189 1455
Cuts at: 4408 618
Size: 1842
Fragments arranged by size:
1842 1455 627 624 339 325 217 189
14
BanII G_rGCy'C
Cuts at: 1391 2709 2815 2951 1391
Size: 1318 106 136 4072
Fragments arranged by size:
4072 1318 136 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2291 3524 4734 2291
Size: 1233 1210 3189
Fragments arranged by size:
3189 1233 1210
BclI T'GATC_A
Cuts at: 1548 2853 1548
Size: 1305 4327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 4243 136
Size: 107 122 71 631 605 382 2189 1525
Fragments arranged by size:
2189 1525 631 605 382 122 107 71
BglII A'GATC_T
Cuts at: 2430 5627 2430
Size: 3197 2435
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2145 2815 3693 4849 1790
Size: 339 16 670 878 1156 2573
Fragments arranged by size:
2573 1156 878 670 339 16
BmgBI CAC'GTC
Cuts at: 1318 2479 1318
Size: 1161 4471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2226 3363 4321
Size: 416 53 83 399 24 809 1137 958
Cuts at: 4321 442
Size: 1753
Fragments arranged by size:
1753 1137 958 809 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2306 2411 2771 4292 1239
Size: 914 153 105 360 1521 2579
Fragments arranged by size:
2579 1521 914 360 153 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2072 2864 3757 4622 4920 2033
Size: 39 792 893 865 298 2745
Fragments arranged by size:
2745 893 865 792 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2537 4295 882
Size: 617 1038 1758 2219
Fragments arranged by size:
2219 1758 1038 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2149 2179 2279 2309 1178
Size: 30 165 30 746 30 100 30 4501
Fragments arranged by size:
4501 746 165 100 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2092 2095 2098 2103 2150 2273 2336
Size: 849 57 3 3 5 47 123 63
Cuts at: 2336 2651 2666 2930 1186
Size: 315 15 264 3888
Fragments arranged by size:
3888 849 315 264 123 63 57 47
15 5 3 3
BseYI C'CCAG_C
Cuts at: 2150 2551 4835 5331 2150
Size: 401 2284 496 2451
Fragments arranged by size:
2451 2284 496 401
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2012 2108 2756 1163
Size: 573 276 96 648 4039
Fragments arranged by size:
4039 648 573 276 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 3693 3778 4849 2691
Size: 1002 85 1071 3474
Fragments arranged by size:
3474 1071 1002 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2145 2691 2709 2815 2951 3693
Size: 399 339 16 546 18 106 136 742
Cuts at: 3693 3778 4849 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 399 339 136 106
85 18 16
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 2604 3521 5251 2604
Size: 917 1730 2985
Fragments arranged by size:
2985 1730 917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2531 2752 2987 844
Size: 1687 221 235 3489
Fragments arranged by size:
3489 1687 235 221
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2619 4127 4301 65
Size: 1183 1371 1508 174 1396
Fragments arranged by size:
1508 1396 1371 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2498 2757 4276 4463 1700
Size: 110 688 259 1519 187 2869
Fragments arranged by size:
2869 1519 688 259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2161 2430 2962 3329 3714 3731 5183 5269
Size: 189 269 532 367 385 17 1452 86
Cuts at: 5269 5281 5627 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 532 385 367 346 269 189
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2040 2178 2634 2040
Size: 138 456 5038
Fragments arranged by size:
5038 456 138
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5080
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 2029 2145 3148 3942 3962 2029
Size: 116 1003 794 20 3699
Fragments arranged by size:
3699 1003 794 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2500 2623 3079
Size: 54 1425 50 275 18 670 123 456
Cuts at: 3079 3968 5597 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 670 456 275 123 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2916 3562 1353
Size: 897 666 646 3423
Fragments arranged by size:
3423 897 666 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2179 4219 4593 4739 387
Size: 1064 138 590 2040 374 146 1280
Fragments arranged by size:
2040 1280 1064 590 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2306 2411 2771 3695 4292 5079
Size: 393 521 153 105 360 924 597 787
Cuts at: 5079 5347 1239
Size: 268 1524
Fragments arranged by size:
1524 924 787 597 521 393 360 268
153 105
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2135
Size: 5632
EcoO109I rG'GnC_Cy
Cuts at: 2068 2811 2812 2068
Size: 743 1 4888
Fragments arranged by size:
4888 743 1
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2292 2599 2292
Size: 307 5325
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 4779 1678
Size: 3101 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2292 2324 4273 4305 4347
Size: 32 135 32 720 32 1949 32 42
Cuts at: 4347 4379 1373
Size: 32 2626
Fragments arranged by size:
2626 1949 720 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2157 2976 3222 669
Size: 988 281 219 819 246 3079
Fragments arranged by size:
3079 988 819 281 246 219
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 2508 3590 3949 4108 518
Size: 648 1311 31 1082 359 159 2042
Fragments arranged by size:
2042 1311 1082 648 359 159 31
MspA1I CmG'CkG
Cuts at: 1606 2461 3048 3725 4873 5118 1606
Size: 855 587 677 1148 245 2120
Fragments arranged by size:
2120 1148 855 677 587 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5080
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2990 4535 1132
Size: 97 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2071 1348
Size: 723 4909
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2731 1108
Size: 1623 4009
PfoI T'CCnGG_A
Cuts at: 2283 2283
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2279 2311 3701 3733 5214 5246 2279
Size: 32 1390 32 1481 32 2665
Fragments arranged by size:
2665 1481 1390 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspOMI G'GGCC_C
Cuts at: 2811 2811
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2980 4115 4796 4987 826
Size: 219 1935 1135 681 191 1471
Fragments arranged by size:
1935 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 2051 2843 3736 4637 4899
Size: 197 926 69 39 792 893 901 262
Cuts at: 4899 820
Size: 1553
Fragments arranged by size:
1553 926 901 893 792 262 197 69
39
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2527 2807 3071 513
Size: 552 1462 280 264 3074
Fragments arranged by size:
3074 1462 552 280 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2543 2738 3659 3676 3829 4014 4459
Size: 1255 963 195 921 17 153 185 445
Cuts at: 4459 325
Size: 1498
Fragments arranged by size:
1498 1255 963 921 445 195 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3878 5590 96
Size: 274 80 33 51 1443 1901 1712 138
Fragments arranged by size:
1901 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI