Restriction map for
pFN21AB9817
(Circular) (Six-base) MAPSORT of: pFN21AB9817.seq Check: 3376 from: 1 to: 5620
pFN21AB9817 5620 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2334 2334
Size: 5620
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 3683 5067 5335 1632
Size: 830 1221 1384 268 1917
Fragments arranged by size:
1917 1384 1221 830 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 2829 4519 5496 1203
Size: 1626 1690 977 1327
Fragments arranged by size:
1690 1626 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 2508 4349 2508
Size: 1841 3779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2619 1184
Size: 1435 4185
AlwNI CAG_nnn'CTG
Cuts at: 2881 3363 4935 2881
Size: 482 1572 3566
Fragments arranged by size:
3566 1572 482
ApaLI G'TGCA_C
Cuts at: 2691 3677 4833 2691
Size: 986 1156 3478
Fragments arranged by size:
3478 1156 986
ApoI r'AATT_y
Cuts at: 1493 2422 2609 2929 3155 3307 5416 1493
Size: 929 187 320 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 320 226 187 152
AseI AT'TA_AT
Cuts at: 160 1032 2460 4174 160
Size: 872 1428 1714 1606
Fragments arranged by size:
1714 1606 1428 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
AvrII C'CTAG_G
Cuts at: 2515 2515
Size: 5620
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2658 928
Size: 971 759 3890
Fragments arranged by size:
3890 971 759
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2695 3681 4837 1790
Size: 905 986 1156 2573
Fragments arranged by size:
2573 1156 986 905
BmgBI CAC'GTC
Cuts at: 1318 2830 1318
Size: 1512 4108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2104 2292 3351
Size: 416 53 83 399 24 687 188 1059
Cuts at: 3351 4309 442
Size: 958 1753
Fragments arranged by size:
1753 1059 958 687 416 399 188 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 4280 1239
Size: 872 2169 2579
Fragments arranged by size:
2579 2169 872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4610 4908 3745
Size: 865 298 4457
Fragments arranged by size:
4457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2555 3411 493
Size: 711 1351 856 2702
Fragments arranged by size:
2702 1351 856 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5620
BseYI C'CCAG_C
Cuts at: 4823 5319 4823
Size: 496 5124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2695 3681 3766 4837 2695
Size: 986 85 1071 3478
Fragments arranged by size:
3478 1071 986 85
BsmI GAATG_Cn'
Cuts at: 2029 2738 3131 3224 2029
Size: 709 393 93 4425
Fragments arranged by size:
4425 709 393 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2516 2516
Size: 5620
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2695 2939 3681 3766 4837 1391
Size: 399 905 244 742 85 1071 2174
Fragments arranged by size:
2174 1071 905 742 399 244 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 2975 844
Size: 1490 641 3489
Fragments arranged by size:
3489 1490 641
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 2330 96
Size: 2234 3386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2733 794
Size: 1939 3681
BstBI TT'CG_AA
Cuts at: 2377 4424 2377
Size: 2047 3573
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2950 3317 3702 3719 5171 5257 5269 5615
Size: 978 367 385 17 1452 86 12 346
Cuts at: 5615 1972
Size: 1977
Fragments arranged by size:
1977 1452 978 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 2420 3136 3930 3950 2420
Size: 716 794 20 4090
Fragments arranged by size:
4090 794 716 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2137 2924 2137
Size: 787 4833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3067 3956 5585
Size: 54 1425 50 275 18 1237 889 1629
Cuts at: 5585 8
Size: 43
Fragments arranged by size:
1629 1425 1237 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 3550 1353
Size: 671 1526 3423
Fragments arranged by size:
3423 1526 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2462 3683 4280 5067 5335 1239
Size: 393 479 351 1221 597 787 268 1524
Fragments arranged by size:
1524 1221 787 597 479 393 351 268
EcoO109I rG'GnC_Cy
Cuts at: 2086 2086
Size: 5620
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
EcoRV GAT'ATC
Cuts at: 2288 2288
Size: 5620
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2193 2225 2494 2526 4261
Size: 32 135 32 621 32 269 32 1735
Cuts at: 4261 4293 4335 4367 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1735 621 269 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 2334 3219 2334
Size: 885 4735
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2043 4734 4918 1305
Size: 738 2691 184 2007
Fragments arranged by size:
2691 2007 738 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3798
Fragments arranged by size:
3798 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3578 3937 4096 518
Size: 648 2412 359 159 2042
Fragments arranged by size:
2412 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3036 3713 4861 5106 1606
Size: 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2877 2972 830
Size: 2047 95 3478
Fragments arranged by size:
3478 2047 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2873 2968 4103 4784 4975 826
Size: 219 1828 95 1135 681 191 1471
Fragments arranged by size:
1828 1471 1135 681 219 191 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4625 4887 820
Size: 197 926 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 2251 3544 5
Size: 47 2199 1293 2081
Fragments arranged by size:
2199 2081 1293 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 2515 3059 513
Size: 552 939 511 544 3074
Fragments arranged by size:
3074 939 552 544 511
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2311 3647 3664 3817 4002 4447 325
Size: 1255 731 1336 17 153 185 445 1498
Fragments arranged by size:
1498 1336 1255 731 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 2330 2832
Size: 274 80 33 51 1443 279 74 502
Cuts at: 2832 2863 3866 5578 96
Size: 31 1003 1712 138
Fragments arranged by size:
1712 1443 1003 502 279 274 138 80
74 51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI