Restriction map for
pFN21AB9816
(Circular) (Six-base) MAPSORT of: pFN21AB9816.seq Check: 2176 from: 1 to: 5605
pFN21AB9816 5605 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2133 2948 1937
Size: 196 815 4594
Fragments arranged by size:
4594 815 196
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 2539 2879 3668 5052 5320 1632
Size: 575 332 340 789 1384 268 1917
Fragments arranged by size:
1917 1384 789 575 340 332 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 2831 4436 2831
Size: 1605 4000
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 3348 4920 3348
Size: 1572 4033
ApaI G_GGCC'C
Cuts at: 2401 2692 2401
Size: 291 5314
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2914 3140 3292 5401 1493
Size: 1421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 1870
Size: 73 987 4545
Fragments arranged by size:
4545 987 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2798 2926 4381 618
Size: 325 217 627 1011 128 1455 1842
Fragments arranged by size:
1842 1455 1011 627 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2401 2692 2790 2924 1391
Size: 1010 291 98 134 4072
Fragments arranged by size:
4072 1010 291 134 98
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2239 2867 928
Size: 971 340 628 3666
Fragments arranged by size:
3666 971 628 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2213 2396 2546
Size: 107 122 71 631 605 541 183 150
Cuts at: 2546 2678 2687 4216 136
Size: 132 9 1529 1525
Fragments arranged by size:
1529 1525 631 605 541 183 150 132
122 107 71 9
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2243 2401 2629 2692 3666 4822 1790
Size: 453 158 228 63 974 1156 2573
Fragments arranged by size:
2573 1156 974 453 228 158 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2207 1056
Size: 1151 4454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2427 2537 2824 4265 1239
Size: 1188 110 287 1441 2579
Fragments arranged by size:
2579 1441 1188 287 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4268 882
Size: 617 2769 2219
Fragments arranged by size:
2769 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2611 3396 493
Size: 711 1407 785 2702
Fragments arranged by size:
2702 1407 785 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2355 2506 2831 4038 4436 4710 4857 5268 2355
Size: 151 325 1207 398 274 147 411 2692
Fragments arranged by size:
2692 1207 411 398 325 274 151 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2714 2744 1178
Size: 30 165 30 1311 30 4039
Fragments arranged by size:
4039 1311 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2684 1186
Size: 1498 4107
BseYI C'CCAG_C
Cuts at: 2056 2782 4808 5304 2056
Size: 726 2026 496 2357
Fragments arranged by size:
2357 2026 726 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2328 1163
Size: 573 592 4440
Fragments arranged by size:
4440 592 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3666 3751 4822 3666
Size: 85 1071 4449
Fragments arranged by size:
4449 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
BsmBI CGTCTCn'nnnn_
Cuts at: 2120 2120
Size: 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2401 2629 2692 2790 2924 3666
Size: 399 453 158 228 63 98 134 742
Cuts at: 3666 3751 4822 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 399 228 158 134
98 85 63
BspEI T'CCGG_A
Cuts at: 2355 5268 2355
Size: 2913 2692
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2019 2169 2960 844
Size: 1175 150 791 3489
Fragments arranged by size:
3489 1175 791 150
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 2637 3492 1394
Size: 429 245 569 855 3507
Fragments arranged by size:
3507 855 569 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2227 2279 2491 2540 2681 2831 4249
Size: 110 417 52 212 49 141 150 1418
Cuts at: 4249 4436 1700
Size: 187 2869
Fragments arranged by size:
2869 1418 417 212 187 150 141 110
52 49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 2827 1184
Size: 1643 3962
BstXI CCAn_nnnn'nTGG
Cuts at: 2451 2451
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600
Size: 963 367 385 17 1452 86 12 346
Cuts at: 5600 1972
Size: 1977
Fragments arranged by size:
1977 1452 963 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 3121 3915 3935 3121
Size: 794 20 4791
Fragments arranged by size:
4791 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2079 2295
Size: 54 1425 50 275 18 213 36 216
Cuts at: 2295 2388 2589 2679 2739 3052 3941 5570 8
Size: 93 201 90 60 313 889 1629 43
Fragments arranged by size:
1629 1425 889 313 275 216 213 201
93 90 60 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3535 1353
Size: 2182 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2671 2791 4192 4566 4712 387
Size: 1064 138 1082 120 1401 374 146 1280
Fragments arranged by size:
1401 1280 1082 1064 374 146 138 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2427 2537 2539 2824 2879 3668
Size: 393 575 220 110 2 285 55 789
Cuts at: 3668 4265 5052 5320 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 789 787 597 575 393 285 268
220 110 55 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2356 2356
Size: 5605
EcoO109I rG'GnC_Cy
Cuts at: 2214 2398 2527 2688 2689 2717 2849 2214
Size: 184 129 161 1 28 132 4970
Fragments arranged by size:
4970 184 161 132 129 28 1
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2204 4752 1678
Size: 526 2548 2531
Fragments arranged by size:
2548 2531 526
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 2519 2551 4246
Size: 32 135 32 589 32 326 32 1695
Cuts at: 4246 4278 4320 4352 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1695 589 326 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2497 2949 3195 669
Size: 988 281 559 452 246 3079
Fragments arranged by size:
3079 988 559 452 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 2415 2757 4719 4903 1305
Size: 876 234 342 1962 184 2007
Fragments arranged by size:
2007 1962 876 342 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 2591 2741 10
Size: 54 1425 343 249 510 150 2874
Fragments arranged by size:
2874 1425 510 343 249 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3563 3922 4081 518
Size: 648 2397 359 159 2042
Fragments arranged by size:
2397 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2824 3021 3698 4846 5091 1606
Size: 1218 197 677 1148 245 2120
Fragments arranged by size:
2120 1218 1148 677 245 197
NaeI GCC'GGC
Cuts at: 2229 2281 2542 2683 2229
Size: 52 261 141 5151
Fragments arranged by size:
5151 261 141 52
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NgoMIV G'CCGG_C
Cuts at: 2227 2279 2540 2681 2227
Size: 52 261 141 5151
Fragments arranged by size:
5151 261 141 52
NheI G'CTAG_C
Cuts at: 1052 2203 1052
Size: 1151 4454
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2476 1348
Size: 1128 4477
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2443 2443
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2717 2849 2527
Size: 190 132 5283
Fragments arranged by size:
5283 190 132
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PspOMI G'GGCC_C
Cuts at: 2397 2688 2397
Size: 291 5314
PstI C_TGCA'G
Cuts at: 830 2957 830
Size: 2127 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SanDI GG'GwC_CC
Cuts at: 2527 2527
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2953 4088 4769 4960 826
Size: 219 1908 1135 681 191 1471
Fragments arranged by size:
1908 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2396 2687 2396
Size: 291 5314
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 2522 1517
Size: 1005 4600
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2586 3044 513
Size: 552 939 333 249 458 3074
Fragments arranged by size:
3074 939 552 458 333 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2665 3632 3649 3802 3987 4432 325
Size: 1255 1085 967 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1085 967 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfoI SrfI SwaI XcmI