Restriction map for
pFN21AB9815
(Circular) (Six-base) MAPSORT of: pFN21AB9815.seq Check: 8955 from: 1 to: 5602
pFN21AB9815 5602 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2602 2602
Size: 5602
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 5049 5317 1632
Size: 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 2837 4501 5478 1203
Size: 1634 1664 977 1327
Fragments arranged by size:
1664 1634 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 2377 4331 2377
Size: 1954 3648
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 2435
Size: 32 5570
AlwNI CAG_nnn'CTG
Cuts at: 2446 3345 4917 2446
Size: 899 1572 3131
Fragments arranged by size:
3131 1572 899
ApaI G_GGCC'C
Cuts at: 2682 2682
Size: 5602
ApaLI G'TGCA_C
Cuts at: 2762 3659 4815 2762
Size: 897 1156 3549
Fragments arranged by size:
3549 1156 897
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AscI GG'CGCG_CC
Cuts at: 2265 2265
Size: 5602
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2143 2240 2635 2690 2927 1870
Size: 73 74 126 97 395 55 237 4545
Fragments arranged by size:
4545 395 237 126 97 74 73 55
BamHI G'GATC_C
Cuts at: 2048 2889 2932 3299 2048
Size: 841 43 367 4351
Fragments arranged by size:
4351 841 367 43
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2470 2792 2923 4378 618
Size: 325 217 627 683 322 131 1455 1842
Fragments arranged by size:
1842 1455 683 627 325 322 217 131
BanII G_rGCy'C
Cuts at: 1391 2024 2144 2682 2751 2921 1391
Size: 633 120 538 69 170 4072
Fragments arranged by size:
4072 633 538 170 120 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BbvCI CC'TCA_GC
Cuts at: 2075 2075
Size: 5602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2400 2434 2846 2880 3793 3827 1903
Size: 34 463 34 412 34 913 34 3678
Fragments arranged by size:
3678 913 463 412 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2694 4213 136
Size: 107 122 71 631 605 1022 1519 1525
Fragments arranged by size:
1525 1519 1022 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 2475 2682 2699 2766 3663 4819 1790
Size: 685 207 17 67 897 1156 2573
Fragments arranged by size:
2573 1156 897 685 207 67 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2812 3333 4291
Size: 416 53 83 399 24 1395 521 958
Cuts at: 4291 442
Size: 1753
Fragments arranged by size:
1753 1395 958 521 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2468 2561 2765 4262 1239
Size: 893 336 93 204 1497 2579
Fragments arranged by size:
2579 1497 893 336 204 93
Bpu10I CC'TnA_GC
Cuts at: 2075 2075
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2395 3393 493
Size: 711 1191 998 2702
Fragments arranged by size:
2702 1191 998 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 3409 3791 4426
Size: 53 83 186 936 792 1084 382 635
Cuts at: 4426 275
Size: 1451
Fragments arranged by size:
1451 1084 936 792 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5377
Fragments arranged by size:
5377 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5602
BseYI C'CCAG_C
Cuts at: 2231 4805 5301 2231
Size: 2574 496 2532
Fragments arranged by size:
2574 2532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 2840 1163
Size: 573 639 465 3925
Fragments arranged by size:
3925 639 573 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2288 3052 3813 4841 1540
Size: 275 186 287 764 761 1028 2301
Fragments arranged by size:
2301 1028 764 761 287 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2751 2766 3663 3748 4819 2024
Size: 727 15 897 85 1071 2807
Fragments arranged by size:
2807 1071 897 727 85 15
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
BsmBI CGTCTCn'nnnn_
Cuts at: 2364 2364
Size: 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2144 2475 2682 2699 2751 2766
Size: 399 234 120 331 207 17 52 15
Cuts at: 2766 2921 3663 3748 4819 1391
Size: 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 331 234 207 155
120 85 52 17 15
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2602 2957 844
Size: 1758 355 3489
Fragments arranged by size:
3489 1758 355
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2461 4097 4271 65
Size: 1183 1213 1636 174 1396
Fragments arranged by size:
1636 1396 1213 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 2151 2219 2265 1925
Size: 226 68 46 5262
Fragments arranged by size:
5262 226 68 46
BssSI C'ACGA_G
Cuts at: 2531 2654 3662 4674 2531
Size: 123 1008 1012 3459
Fragments arranged by size:
3459 1012 1008 123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2048 2889 2932 3299 3684 3701 5153 5239
Size: 76 841 43 367 385 17 1452 86
Cuts at: 5239 5251 5597 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 841 385 367 346 86 76
43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2025 2117 2259 513
Size: 552 960 92 142 3856
Fragments arranged by size:
3856 960 552 142 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2225 3118 3912 3932 2225
Size: 893 794 20 3895
Fragments arranged by size:
3895 893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2285 3049 3938
Size: 54 1425 50 275 18 455 764 889
Cuts at: 3938 5567 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 764 455 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2285 3049 1537
Size: 275 473 764 4090
Fragments arranged by size:
4090 764 473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2874 3532 1353
Size: 1521 658 3423
Fragments arranged by size:
3423 1521 658
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2022 2749 2022
Size: 727 4875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2468 2561 2765 3665 4262 5049
Size: 393 500 336 93 204 900 597 787
Cuts at: 5049 5317 1239
Size: 268 1524
Fragments arranged by size:
1524 900 787 597 500 393 336 268
204 93
EcoO109I rG'GnC_Cy
Cuts at: 2130 2443 2687 2130
Size: 313 244 5045
Fragments arranged by size:
5045 313 244
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2043 2174 2248 2277 2518 4749 1678
Size: 365 131 74 29 241 2231 2531
Fragments arranged by size:
2531 2231 365 241 131 74 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 4243 4275 4317
Size: 32 135 32 791 32 1848 32 42
Cuts at: 4317 4349 1373
Size: 32 2626
Fragments arranged by size:
2626 1848 791 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 4716 4900 1305
Size: 762 2649 184 2007
Fragments arranged by size:
2649 2007 762 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3780
Fragments arranged by size:
3780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3560 3919 4078 518
Size: 648 2394 359 159 2042
Fragments arranged by size:
2394 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2079 2119 2261 2389 2524 2574 3018
Size: 421 52 40 142 128 135 50 444
Cuts at: 3018 3695 4843 5088 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 444 421 245 142 135
128 52 50 40
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5050
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2841 2863 2960 4505 1132
Size: 97 1612 22 97 1545 2229
Fragments arranged by size:
2229 1612 1545 97 97 22
PasI CC'CwG_GG
Cuts at: 1348 2044 2135 2329 1348
Size: 696 91 194 4621
Fragments arranged by size:
4621 696 194 91
PciI A'CATG_T
Cuts at: 2837 4501 2837
Size: 1664 3938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2446 1108
Size: 1338 4264
PfoI T'CCnGG_A
Cuts at: 2051 2051
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PmlI CAC'GTG
Cuts at: 2395 2395
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2443 2443
Size: 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspOMI G'GGCC_C
Cuts at: 2678 2678
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2297 2435 2523 2954 830
Size: 1467 138 88 431 3478
Fragments arranged by size:
3478 1467 431 138 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
PvuII CAG'CTG
Cuts at: 2079 2079
Size: 5602
SacI G_AGCT'C
Cuts at: 2024 2751 2024
Size: 727 4875
SacII CC_GC'GG
Cuts at: 2028 2120 2262 2028
Size: 92 142 5368
Fragments arranged by size:
5368 142 92
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 2801 3850 5562 2801
Size: 1049 1712 2841
Fragments arranged by size:
2841 1712 1049
SfcI C'TryA_G
Cuts at: 826 1045 2125 2293 2431 2519 2827 2950 4085
Size: 219 1080 168 138 88 308 123 1135
Cuts at: 4085 4766 4957 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1080 681 308 219 191 168
138 123 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2145 2929 1872
Size: 273 784 4545
Fragments arranged by size:
4545 784 273
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SrfI GCCC'GGGC
Cuts at: 2145 2145
Size: 5602
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2563 3041 513
Size: 552 1498 478 3074
Fragments arranged by size:
3074 1498 552 478
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2799 3848 5560
Size: 274 80 33 51 1443 822 1049 1712
Cuts at: 5560 96
Size: 138
Fragments arranged by size:
1712 1443 1049 822 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2143 2927 1870
Size: 273 784 4545
Fragments arranged by size:
4545 784 273
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SfoI SwaI XcmI