Restriction map for
pFN21AB9808
(Circular) (Six-base) MAPSORT of: pFN21AB9808.seq Check: 1343 from: 1 to: 5428
pFN21AB9808 5428 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 3491 4875 5143 1632
Size: 476 1383 1384 268 1917
Fragments arranged by size:
1917 1384 1383 476 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 2188 4327 5304 1203
Size: 985 2139 977 1327
Fragments arranged by size:
2139 1327 985 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 2311 3171 4743 2311
Size: 860 1572 2996
Fragments arranged by size:
2996 1572 860
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2399 2737 2963 3115 5224 1493
Size: 906 338 226 152 2109 1697
Fragments arranged by size:
2109 1697 906 338 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 1870
Size: 73 810 4545
Fragments arranged by size:
4545 810 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2568 2749 4204 618
Size: 325 217 627 781 181 1455 1842
Fragments arranged by size:
1842 1455 781 627 325 217 181
BanII G_rGCy'C
Cuts at: 1391 2158 2747 1391
Size: 767 589 4072
Fragments arranged by size:
4072 767 589
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 2028 1548
Size: 480 4948
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2702 2845 2702
Size: 143 5285
Bme1580I G_kGCm'C
Cuts at: 1790 3489 4645 1790
Size: 1699 1156 2573
Fragments arranged by size:
2573 1699 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3159 4117 442
Size: 416 53 83 399 24 1742 958 1753
Fragments arranged by size:
1753 1742 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4088 1239
Size: 2849 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2525 4091 882
Size: 617 1026 1566 2219
Fragments arranged by size:
2219 1566 1026 617
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3235 3617 4252 275
Size: 53 83 186 936 1702 382 635 1451
Fragments arranged by size:
1702 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2673 3861 4259 4533 4680 5091 2673
Size: 1188 398 274 147 411 3010
Fragments arranged by size:
3010 1188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2266 2296 1178
Size: 30 165 30 863 30 4310
Fragments arranged by size:
4310 863 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5428
BseYI C'CCAG_C
Cuts at: 2277 4631 5127 2277
Size: 2354 496 2578
Fragments arranged by size:
2578 2354 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2169 1163
Size: 573 433 4422
Fragments arranged by size:
4422 573 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2878 3639 4667 1540
Size: 275 186 877 761 1028 2301
Fragments arranged by size:
2301 1028 877 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2703 3489 3574 4645 2703
Size: 786 85 1071 3486
Fragments arranged by size:
3486 1071 786 85
BsmI GAATG_Cn'
Cuts at: 2128 2939 3032 2128
Size: 811 93 4524
Fragments arranged by size:
4524 811 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2703 2747 3489 3574 4645 1391
Size: 399 368 545 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 368 85 44
BspEI T'CCGG_A
Cuts at: 2673 5091 2673
Size: 2418 3010
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2783 844
Size: 1939 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 1394
Size: 429 1492 3507
Fragments arranged by size:
3507 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2083 3923 4097 65
Size: 1183 835 1840 174 1396
Fragments arranged by size:
1840 1396 1183 835 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4072 4259 1700
Size: 110 2262 187 2869
Fragments arranged by size:
2869 2262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 3488 4500 3488
Size: 1012 4416
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2308 2758 3125 3510 3527 4979 5065 5077
Size: 336 450 367 385 17 1452 86 12
Cuts at: 5077 5423 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 450 385 367 346 336 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 513
Size: 552 1575 3301
Fragments arranged by size:
3301 1575 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2139 2944 3738 3758 2139
Size: 805 794 20 3809
Fragments arranged by size:
3809 805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2236 2732 2236
Size: 496 4932
DraIII CAC_nnn'GTG
Cuts at: 2261 2261
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2321 4435 809
Size: 507 1005 2114 1802
Fragments arranged by size:
2114 1802 1005 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2875 3764 5393
Size: 54 1425 50 275 18 1045 889 1629
Cuts at: 5393 8
Size: 43
Fragments arranged by size:
1629 1425 1045 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2716 3358 1353
Size: 1363 642 3423
Fragments arranged by size:
3423 1363 642
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2072 4015 4389 4535 387
Size: 1064 138 483 1943 374 146 1280
Fragments arranged by size:
1943 1280 1064 483 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 3491 4088 4875 5143 1239
Size: 393 476 1383 597 787 268 1524
Fragments arranged by size:
1524 1383 787 597 476 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2344 2344
Size: 5428
EcoO109I rG'GnC_Cy
Cuts at: 2545 2545
Size: 5428
EcoRI G'AATT_C
Cuts at: 2399 2737 3115 2399
Size: 338 378 4712
Fragments arranged by size:
4712 378 338
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2166 2636 4575 1678
Size: 488 470 1939 2531
Fragments arranged by size:
2531 1939 488 470
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2082 2114 4069 4101 4143
Size: 32 135 32 510 32 1955 32 42
Cuts at: 4143 4175 1373
Size: 32 2626
Fragments arranged by size:
2626 1955 510 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 2772 3018 669
Size: 988 281 622 212 246 3079
Fragments arranged by size:
3079 988 622 281 246 212
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5428
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 2188 5304 2188
Size: 3116 2312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4542 4726 1305
Size: 3237 184 2007
Fragments arranged by size:
3237 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2216 3386 3745 3904 518
Size: 648 843 207 1170 359 159 2042
Fragments arranged by size:
2042 1170 843 648 359 207 159
MspA1I CmG'CkG
Cuts at: 1606 2174 2565 2844 3521 4669 4914 1606
Size: 568 391 279 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 568 391 279 245
NcoI C'CATG_G
Cuts at: 513 1065 2640 513
Size: 552 1575 3301
Fragments arranged by size:
3301 1575 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2786 4331 1132
Size: 97 1557 1545 2229
Fragments arranged by size:
2229 1557 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2545 2545
Size: 5428
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2089 2299 2780 830
Size: 1259 210 481 3478
Fragments arranged by size:
3478 1259 481 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SanDI GG'GwC_CC
Cuts at: 2545 2545
Size: 5428
SapI GCTCTTCn'nnn_
Cuts at: 2716 2716
Size: 5428
SbfI CC_TGCA'GG
Cuts at: 2089 2780 2089
Size: 691 4737
ScaI AGT'ACT
Cuts at: 2522 3676 5388 2522
Size: 1154 1712 2562
Fragments arranged by size:
2562 1712 1154
SfcI C'TryA_G
Cuts at: 826 1045 2085 2295 2776 3911 4592 4783 826
Size: 219 1040 210 481 1135 681 191 1471
Fragments arranged by size:
1471 1135 1040 681 481 219 210 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3532 4433 4695 820
Size: 197 926 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2640 2867 513
Size: 552 1575 227 3074
Fragments arranged by size:
3074 1575 552 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2291 3455 3472 3625 3810 4255 325
Size: 1255 711 1164 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1164 711 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 3674 5386
Size: 274 80 33 51 1443 543 1154 1712
Cuts at: 5386 96
Size: 138
Fragments arranged by size:
1712 1443 1154 543 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2266 2298 5388 5420 2266
Size: 32 3090 32 2274
Fragments arranged by size:
3090 2274 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2417 1316
Size: 1101 4327
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI XcmI