Restriction map for
pFN21AB9805
(Circular) (Six-base) MAPSORT of: pFN21AB9805.seq Check: 7683 from: 1 to: 5380
pFN21AB9805 5380 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 1937
Size: 786 4594
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3443 4827 5095 1632
Size: 1811 1384 268 1917
Fragments arranged by size:
1917 1811 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 2034 4279 5256 1203
Size: 831 2245 977 1327
Fragments arranged by size:
2245 1327 977 831
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 2476 2524 3123 4695 2476
Size: 48 599 1572 3161
Fragments arranged by size:
3161 1572 599 48
ApaI G_GGCC'C
Cuts at: 2138 2610 2138
Size: 472 4908
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2689 2915 3067 5176 1493
Size: 1196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1196 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2705 1870
Size: 73 194 568 4545
Fragments arranged by size:
4545 568 194 73
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2158 2490 2594 2701 4156
Size: 325 217 627 371 332 104 107 1455
Cuts at: 4156 618
Size: 1842
Fragments arranged by size:
1842 1455 627 371 332 325 217 107
104
BanII G_rGCy'C
Cuts at: 1391 2016 2138 2610 2699 1391
Size: 625 122 472 89 4072
Fragments arranged by size:
4072 625 472 122 89
BbeI G_GCGC'C
Cuts at: 2162 2494 2598 2162
Size: 332 104 4944
Fragments arranged by size:
4944 332 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3571 3605 1903
Size: 34 1634 34 3678
Fragments arranged by size:
3678 1634 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 4482 3272
Size: 1210 4170
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 2142 3991
Size: 107 122 71 631 605 418 52 1849
Cuts at: 3991 136
Size: 1525
Fragments arranged by size:
1849 1525 631 605 418 122 107 71
52
BglII A'GATC_T
Cuts at: 5375 5375
Size: 5380
BlpI GC'TnA_GC
Cuts at: 2312 2797 2312
Size: 485 4895
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2582 2610 3441 4597 1790
Size: 348 444 28 831 1156 2573
Fragments arranged by size:
2573 1156 831 444 348 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 4069 442
Size: 416 53 83 399 24 1694 958 1753
Fragments arranged by size:
1753 1694 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4040 1239
Size: 2801 2579
Bpu10I CC'TnA_GC
Cuts at: 2181 2181
Size: 5380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 3505 4370 4668 2000
Size: 1505 865 298 2712
Fragments arranged by size:
2712 1505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 2307 4043 882
Size: 617 585 223 1736 2219
Fragments arranged by size:
2219 1736 617 585 223
BsaAI yAC'GTr
Cuts at: 493 1204 3171 493
Size: 711 1967 2702
Fragments arranged by size:
2702 1967 711
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2159 2226 2491 2555
Size: 53 83 186 936 626 67 265 64
Cuts at: 2555 2595 2642 3187 3569 4204 275
Size: 40 47 545 382 635 1451
Fragments arranged by size:
1451 936 635 626 545 382 265 186
83 67 64 53 47 40
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5155
Fragments arranged by size:
5155 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5380
BseYI C'CCAG_C
Cuts at: 2176 4583 5079 2176
Size: 2407 496 2477
Fragments arranged by size:
2477 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2148 1163
Size: 573 412 4395
Fragments arranged by size:
4395 573 412
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2288 2830 3591 4619 1540
Size: 275 186 287 542 761 1028 2301
Fragments arranged by size:
2301 1028 761 542 287 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 3441 3526 4597 2016
Size: 1425 85 1071 2799
Fragments arranged by size:
2799 1425 1071 85
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2138 2582 2610 2699 3441 3526
Size: 399 226 122 444 28 89 742 85
Cuts at: 3526 4597 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 444 399 226 122 89
85 28
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 844
Size: 1891 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2211 2332 3267 1394
Size: 429 388 121 935 3507
Fragments arranged by size:
3507 935 429 388 121
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2677 4024 4211 1700
Size: 110 867 1347 187 2869
Fragments arranged by size:
2869 1347 867 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 2324 1925
Size: 399 4981
BssSI C'ACGA_G
Cuts at: 2669 3440 4452 2669
Size: 771 1012 3597
Fragments arranged by size:
3597 1012 771
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5380
BstYI r'GATC_y
Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375
Size: 738 367 385 17 1452 86 12 346
Cuts at: 5375 1972
Size: 1977
Fragments arranged by size:
1977 1452 738 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 2116 2154 513
Size: 552 939 49 63 38 3739
Fragments arranged by size:
3739 939 552 63 49 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2896 3690 3710 2896
Size: 794 20 4566
Fragments arranged by size:
4566 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5345
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DraIII CAC_nnn'GTG
Cuts at: 2577 2577
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4387 809
Size: 507 3071 1802
Fragments arranged by size:
3071 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2285 2526
Size: 54 1425 50 275 18 177 278 241
Cuts at: 2526 2563 2827 3716 5345 8
Size: 37 264 889 1629 43
Fragments arranged by size:
1629 1425 889 278 275 264 241 177
54 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2285 2827 1537
Size: 275 473 542 4090
Fragments arranged by size:
4090 542 473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3310 1353
Size: 1957 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2204 2285 3967 4341 4487 387
Size: 1064 138 615 81 1682 374 146 1280
Fragments arranged by size:
1682 1280 1064 615 374 146 138 81
EcoICRI GAG'CTC
Cuts at: 2014 2014
Size: 5380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3443 4040 4827 5095 1239
Size: 393 1811 597 787 268 1524
Fragments arranged by size:
1811 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2462 2510 2462
Size: 48 5332
EcoO109I rG'GnC_Cy
Cuts at: 2473 2521 2606 2607 2473
Size: 48 85 1 5246
Fragments arranged by size:
5246 85 48 1
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
FspI TGC'GCA
Cuts at: 2411 3886 2411
Size: 1475 3905
HaeII r_GCGC'y
Cuts at: 1678 2162 2494 2598 4527 1678
Size: 484 332 104 1929 2531
Fragments arranged by size:
2531 1929 484 332 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2285 2317 2367
Size: 32 135 32 431 32 250 32 50
Cuts at: 2367 2399 4021 4053 4095 4127 1373
Size: 32 1622 32 42 32 2626
Fragments arranged by size:
2626 1622 431 250 135 50 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2724 2970 669
Size: 988 281 786 246 3079
Fragments arranged by size:
3079 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KasI G'GCGC_C
Cuts at: 2158 2490 2594 2158
Size: 332 104 4944
Fragments arranged by size:
4944 332 104
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 2034 5256 2034
Size: 3222 2158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4494 4678 1305
Size: 3189 184 2007
Fragments arranged by size:
3189 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3558
Fragments arranged by size:
3558 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3338 3697 3856 518
Size: 648 2172 359 159 2042
Fragments arranged by size:
2172 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2156 2199 2239 2299 2796 3473 4621
Size: 512 38 43 40 60 497 677 1148
Cuts at: 4621 4866 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 512 497 245 60 43
40 38
NarI GG'CG_CC
Cuts at: 2159 2491 2595 2159
Size: 332 104 4944
Fragments arranged by size:
4944 332 104
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3889
Fragments arranged by size:
3889 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2285 2827 2285
Size: 542 4838
NspI r_CATG'y
Cuts at: 1132 1229 2344 2738 4283 1132
Size: 97 1115 394 1545 2229
Fragments arranged by size:
2229 1545 1115 394 97
PasI CC'CwG_GG
Cuts at: 1348 2602 1348
Size: 1254 4126
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2524 1108
Size: 1416 3964
PfoI T'CCnGG_A
Cuts at: 2636 2636
Size: 5380
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PspOMI G'GGCC_C
Cuts at: 2134 2606 2134
Size: 472 4908
PstI C_TGCA'G
Cuts at: 830 2732 830
Size: 1902 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
SacI G_AGCT'C
Cuts at: 2016 2016
Size: 5380
SacII CC_GC'GG
Cuts at: 2119 2157 2119
Size: 38 5342
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SfcI C'TryA_G
Cuts at: 826 1045 2728 3863 4544 4735 826
Size: 219 1683 1135 681 191 1471
Fragments arranged by size:
1683 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2160 2492 2596 2160
Size: 332 104 4944
Fragments arranged by size:
4944 332 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2139 2707 1872
Size: 267 568 4545
Fragments arranged by size:
4545 568 267
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 3484 4385 4647 820
Size: 197 926 72 1469 901 262 1553
Fragments arranged by size:
1553 1469 926 901 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2344 2738 1132
Size: 1212 394 3774
Fragments arranged by size:
3774 1212 394
SrfI GCCC'GGGC
Cuts at: 2139 2139
Size: 5380
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5380
StyI C'CwwG_G
Cuts at: 513 1065 2004 2819 513
Size: 552 939 815 3074
Fragments arranged by size:
3074 939 815 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3407 3424 3577 3762 4207 325
Size: 1255 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5340 5372 5340
Size: 32 5348
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2137 2705 1870
Size: 267 568 4545
Fragments arranged by size:
4545 568 267
XmnI GAAnn'nnTTC
Cuts at: 3509 3509
Size: 5380
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI
SfiI SwaI XcmI