Restriction map for
pFN21AB9791
(Circular) (Six-base) MAPSORT of: pFN21AB9791.seq Check: 2596 from: 1 to: 5335
pFN21AB9791 5335 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2207 2453 2207
Size: 246 5089
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3398 4782 5050 1632
Size: 1766 1384 268 1917
Fragments arranged by size:
1917 1766 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 4234 5211 1203
Size: 3031 977 1327
Fragments arranged by size:
3031 1327 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AlwNI CAG_nnn'CTG
Cuts at: 2310 3078 4650 2310
Size: 768 1572 2995
Fragments arranged by size:
2995 1572 768
ApaI G_GGCC'C
Cuts at: 2172 2172
Size: 5335
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2644 2870 3022 5131 1493
Size: 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 2455 2660 1870
Size: 73 193 319 205 4545
Fragments arranged by size:
4545 319 205 193 73
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2219 2374 2656 4111
Size: 325 217 627 369 63 155 282 1455
Cuts at: 4111 618
Size: 1842
Fragments arranged by size:
1842 1455 627 369 325 282 217 155
63
BanII G_rGCy'C
Cuts at: 1391 2172 2654 1391
Size: 781 482 4072
Fragments arranged by size:
4072 781 482
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4364
BbvCI CC'TCA_GC
Cuts at: 2442 2615 2442
Size: 173 5162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2360 2394 3526 3560 1903
Size: 34 50 34 339 34 1132 34 3678
Fragments arranged by size:
3678 1132 339 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2568 2625 3946
Size: 107 122 71 631 605 896 57 1321
Cuts at: 3946 136
Size: 1525
Fragments arranged by size:
1525 1321 896 631 605 122 107 71
57
BglII A'GATC_T
Cuts at: 5330 5330
Size: 5335
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 2161 2172 2224 3396 4552 1790
Size: 371 11 52 1172 1156 2573
Fragments arranged by size:
2573 1172 1156 371 52 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2052 2509 3066
Size: 416 53 83 399 24 635 457 557
Cuts at: 3066 4024 442
Size: 958 1753
Fragments arranged by size:
1753 958 635 557 457 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 3995 1239
Size: 971 1785 2579
Fragments arranged by size:
2579 1785 971
Bpu10I CC'TnA_GC
Cuts at: 2442 2615 2442
Size: 173 5162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3460 4325 4623 3460
Size: 865 298 4172
Fragments arranged by size:
4172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3998 882
Size: 617 2499 2219
Fragments arranged by size:
2499 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3126 493
Size: 711 1922 2702
Fragments arranged by size:
2702 1922 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3142 3524 4159 275
Size: 53 83 186 936 1609 382 635 1451
Fragments arranged by size:
1609 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3768 4166 4440 4587 4998 3768
Size: 398 274 147 411 4105
Fragments arranged by size:
4105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 1178
Size: 30 165 30 711 30 4369
Fragments arranged by size:
4369 711 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2042 2603 1186
Size: 844 12 561 3918
Fragments arranged by size:
3918 844 561 12
BseYI C'CCAG_C
Cuts at: 2165 2195 2310 4538 5034 2165
Size: 30 115 2228 496 2466
Fragments arranged by size:
2466 2228 496 115 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2244 2590 1163
Size: 573 508 346 3908
Fragments arranged by size:
3908 573 508 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3546 4574 1540
Size: 275 186 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3396 3481 4552 3396
Size: 85 1071 4179
Fragments arranged by size:
4179 1071 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2172 2224 2654 3396 3481 4552
Size: 399 371 11 52 430 742 85 1071
Cuts at: 4552 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 430 399 371 85 52
11
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 2453 2690 844
Size: 1363 246 237 3489
Fragments arranged by size:
3489 1363 246 237
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 1394
Size: 429 1399 3507
Fragments arranged by size:
3507 1399 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2093 3830 4004 65
Size: 1183 845 1737 174 1396
Fragments arranged by size:
1737 1396 1183 845 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2238 3979 4166 1700
Size: 110 428 1741 187 2869
Fragments arranged by size:
2869 1741 428 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5335
BstBI TT'CG_AA
Cuts at: 2584 4139 2584
Size: 1555 3780
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2665 3032 3417 3434 4886 4972 4984 5330
Size: 693 367 385 17 1452 86 12 346
Cuts at: 5330 1972
Size: 1977
Fragments arranged by size:
1977 1452 693 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4783
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2310 2590 487
Size: 683 56 1084 280 3232
Fragments arranged by size:
3232 1084 683 280 56
BtsI GCAGTG_nn'
Cuts at: 2592 2851 3645 3665 2592
Size: 259 794 20 4262
Fragments arranged by size:
4262 794 259 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4342 809
Size: 507 3026 1802
Fragments arranged by size:
3026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2626 2782
Size: 54 1425 50 275 18 396 400 156
Cuts at: 2782 3671 5300 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 400 396 275 156 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 3265 1353
Size: 833 1079 3423
Fragments arranged by size:
3423 1079 833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2390 3922 4296 4442 387
Size: 1064 138 801 1532 374 146 1280
Fragments arranged by size:
1532 1280 1064 801 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 3398 3995 4782 5050 1239
Size: 393 578 1188 597 787 268 1524
Fragments arranged by size:
1524 1188 787 597 578 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 2189
Size: 5335
EcoO109I rG'GnC_Cy
Cuts at: 2122 2169 2199 2122
Size: 47 30 5258
Fragments arranged by size:
5258 47 30
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 3841 3841
Size: 5335
HaeII r_GCGC'y
Cuts at: 1678 4482 1678
Size: 2804 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 3976 4008 4050
Size: 32 135 32 542 32 1830 32 42
Cuts at: 4050 4082 1373
Size: 32 2626
Fragments arranged by size:
2626 1830 542 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2067 2934 2067
Size: 867 4468
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3293 3652 3811 518
Size: 648 2127 359 159 2042
Fragments arranged by size:
2127 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2333 2384 2424 2549 2751 3428 4576 4821
Size: 727 51 40 125 202 677 1148 245
Cuts at: 4821 1606
Size: 2120
Fragments arranged by size:
2120 1148 727 677 245 202 125 51
40
NaeI GCC'GGC
Cuts at: 2240 2240
Size: 5335
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4783
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NgoMIV G'CCGG_C
Cuts at: 2238 2238
Size: 5335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2693 4238 1132
Size: 97 1464 1545 2229
Fragments arranged by size:
2229 1545 1464 97
PasI CC'CwG_GG
Cuts at: 1348 2557 1348
Size: 1209 4126
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5335
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4917 4949 3404
Size: 32 1481 32 3790
Fragments arranged by size:
3790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2122
Size: 5335
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PspOMI G'GGCC_C
Cuts at: 2168 2168
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2383 2687 830
Size: 1553 304 3478
Fragments arranged by size:
3478 1553 304
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
PvuII CAG'CTG
Cuts at: 2384 2384
Size: 5335
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SapI GCTCTTCn'nnn_
Cuts at: 2186 2186
Size: 5335
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 2408 3583 5295 2408
Size: 1175 1712 2448
Fragments arranged by size:
2448 1712 1175
SfcI C'TryA_G
Cuts at: 826 1045 2379 2683 3818 4499 4690 826
Size: 219 1334 304 1135 681 191 1471
Fragments arranged by size:
1471 1334 1135 681 304 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2457 2662 1872
Size: 585 205 4545
Fragments arranged by size:
4545 585 205
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3439 4340 4602 820
Size: 197 926 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2693 1132
Size: 1561 3774
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5335
StyI C'CwwG_G
Cuts at: 513 1065 2774 513
Size: 552 1709 3074
Fragments arranged by size:
3074 1709 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2302 3362 3379 3532 3717 4162 325
Size: 1255 722 1060 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1060 722 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2406 3581 5293
Size: 274 80 33 51 1443 429 1175 1712
Cuts at: 5293 96
Size: 138
Fragments arranged by size:
1712 1443 1175 429 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5295 5327 5295
Size: 32 5303
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5335
XmaI C'CCGG_G
Cuts at: 1870 2455 2660 1870
Size: 585 205 4545
Fragments arranged by size:
4545 585 205
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI