Restriction map for
pFN21AB9783
(Circular) (Six-base) MAPSORT of: pFN21AB9783.seq Check: 2510 from: 1 to: 5458
pFN21AB9783 5458 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2020 2650 2020
Size: 630 4828
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2550 3268 278
Size: 53 83 186 936 1014 718 2468
Fragments arranged by size:
2468 1014 936 718 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2613 3521 4905 5173 1632
Size: 981 908 1384 268 1917
Fragments arranged by size:
1917 1384 981 908 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2285 1184
Size: 1101 4357
AlwNI CAG_nnn'CTG
Cuts at: 2254 3201 4773 2254
Size: 947 1572 2939
Fragments arranged by size:
2939 1572 947
ApaI G_GGCC'C
Cuts at: 2393 2487 2628 2393
Size: 94 141 5223
Fragments arranged by size:
5223 141 94
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2767 2993 3145 5254 1493
Size: 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2783 1870
Size: 73 840 4545
Fragments arranged by size:
4545 840 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2368 2398 2779 4234 618
Size: 325 217 627 581 30 381 1455 1842
Fragments arranged by size:
1842 1455 627 581 381 325 217 30
BanII G_rGCy'C
Cuts at: 1391 2279 2345 2393 2487 2520 2628 2777 1391
Size: 888 66 48 94 33 108 149 4072
Fragments arranged by size:
4072 888 149 108 94 66 48 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4487
BbvCI CC'TCA_GC
Cuts at: 2724 2724
Size: 5458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2331 2482 2615
Size: 107 122 71 631 605 659 151 133
Cuts at: 2615 4069 136
Size: 1454 1525
Fragments arranged by size:
1525 1454 659 631 605 151 133 122
107 71
BglII A'GATC_T
Cuts at: 5453 5453
Size: 5458
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 2327 2371 2393 2403 2487 2628 3519 4675
Size: 537 44 22 10 84 141 891 1156
Cuts at: 4675 1790
Size: 2573
Fragments arranged by size:
2573 1156 891 537 141 84 44 22
10
BmgBI CAC'GTC
Cuts at: 1318 2322 1318
Size: 1004 4454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 4147 442
Size: 416 53 83 399 24 1772 958 1753
Fragments arranged by size:
1772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 4118 1239
Size: 1274 1605 2579
Fragments arranged by size:
2579 1605 1274
Bpu10I CC'TnA_GC
Cuts at: 2373 2724 2373
Size: 351 5107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2638 3583 4448 4746 2638
Size: 945 865 298 3350
Fragments arranged by size:
3350 945 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2187 2733 4121 882
Size: 617 688 546 1388 2219
Fragments arranged by size:
2219 1388 688 617 546
BsaAI yAC'GTr
Cuts at: 493 1204 2127 3249 493
Size: 711 923 1122 2702
Fragments arranged by size:
2702 1122 923 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2547 3265 3647 4282
Size: 53 83 186 936 1014 718 382 635
Cuts at: 4282 275
Size: 1451
Fragments arranged by size:
1451 1014 936 718 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2052 3891 4289 4563 4710 5121 2052
Size: 1839 398 274 147 411 2389
Fragments arranged by size:
2389 1839 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2631 2661 1178
Size: 30 165 30 1228 30 3975
Fragments arranged by size:
3975 1228 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2201 2612 1186
Size: 1006 9 411 4032
Fragments arranged by size:
4032 1006 411 9
BseYI C'CCAG_C
Cuts at: 4661 5157 4661
Size: 496 4962
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4885
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3669 4697 1540
Size: 275 186 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2279 2520 3519 3604 4675 2145
Size: 134 241 999 85 1071 2928
Fragments arranged by size:
2928 1071 999 241 134 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2279 2327 2345 2371 2393 2403
Size: 399 355 134 48 18 26 22 10
Cuts at: 2403 2487 2520 2628 2777 3519 3604 4675 1391
Size: 84 33 108 149 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 149 134 108
85 84 48 33 26 22 18 10
BspEI T'CCGG_A
Cuts at: 2052 5121 2052
Size: 3069 2389
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2020 2277 2457 2650 2813 844
Size: 1176 257 180 193 163 3489
Fragments arranged by size:
3489 1176 257 193 180 163
BsrBI CCG'CTC
Cuts at: 1394 1823 2419 3345 1394
Size: 429 596 926 3507
Fragments arranged by size:
3507 926 596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2536 4102 4289 1700
Size: 110 726 1566 187 2869
Fragments arranged by size:
2869 1566 726 187 110
BsrGI T'GTAC_A
Cuts at: 96 2256 96
Size: 2160 3298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5458
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 2662 1184
Size: 1478 3980
BstYI r'GATC_y
Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453
Size: 816 367 385 17 1452 86 12 346
Cuts at: 5453 1972
Size: 1977
Fragments arranged by size:
1977 1452 816 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2346 2346
Size: 5458
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2479 513
Size: 552 939 36 439 3492
Fragments arranged by size:
3492 939 552 439 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2752 2974 3768 3788 2752
Size: 222 794 20 4422
Fragments arranged by size:
4422 794 222 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2616 2905 3794
Size: 54 1425 50 275 18 786 289 889
Cuts at: 3794 5423 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 786 289 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2210 2525 2589 3388 1353
Size: 780 77 315 64 799 3423
Fragments arranged by size:
3423 799 780 315 77 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4045 4419 4565 387
Size: 1064 138 2456 374 146 1280
Fragments arranged by size:
2456 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2277 2518 2277
Size: 241 5217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2613 3521 4118 4905 5173 1239
Size: 393 881 100 908 597 787 268 1524
Fragments arranged by size:
1524 908 881 787 597 393 268 100
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 2618
Size: 5458
EcoO109I rG'GnC_Cy
Cuts at: 2389 2390 2624 2625 2389
Size: 1 234 1 5222
Fragments arranged by size:
5222 234 1 1
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 2410 2326
Size: 84 5374
FspI TGC'GCA
Cuts at: 2583 3964 2583
Size: 1381 4077
HaeII r_GCGC'y
Cuts at: 1678 4605 1678
Size: 2927 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2502 2534 4099
Size: 32 135 32 614 32 284 32 1565
Cuts at: 4099 4131 4173 4205 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1565 614 284 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4572 4756 1305
Size: 3267 184 2007
Fragments arranged by size:
3267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3416 3775 3934 518
Size: 648 2250 359 159 2042
Fragments arranged by size:
2250 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 2337 2481 2723 2874 3551 4699 4944
Size: 645 86 144 242 151 677 1148 245
Cuts at: 4944 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 645 245 242 151 144
86
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3967
Fragments arranged by size:
3967 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2816 4361 1132
Size: 97 1587 1545 2229
Fragments arranged by size:
2229 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5458
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PmlI CAC'GTG
Cuts at: 2127 2127
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2018 2050 3527 3559 5040 5072 2018
Size: 32 1477 32 1481 32 2404
Fragments arranged by size:
2404 1481 1477 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5458
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PspOMI G'GGCC_C
Cuts at: 2389 2483 2624 2389
Size: 94 141 5223
Fragments arranged by size:
5223 141 94
PstI C_TGCA'G
Cuts at: 830 2250 2529 2556 2810 830
Size: 1420 279 27 254 3478
Fragments arranged by size:
3478 1420 279 254 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
PvuII CAG'CTG
Cuts at: 2723 2723
Size: 5458
SacI G_AGCT'C
Cuts at: 2279 2520 2279
Size: 241 5217
SacII CC_GC'GG
Cuts at: 2482 2482
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SapI GCTCTTCn'nnn_
Cuts at: 2133 2525 2133
Size: 392 5066
SbfI CC_TGCA'GG
Cuts at: 2529 2556 2810 2529
Size: 27 254 5177
Fragments arranged by size:
5177 254 27
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SfcI C'TryA_G
Cuts at: 826 1045 2246 2525 2552 2806 3941 4622 4813
Size: 219 1201 279 27 254 1135 681 191
Cuts at: 4813 826
Size: 1471
Fragments arranged by size:
1471 1201 1135 681 279 254 219 191
27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2653 3562 4463 4725 820
Size: 197 926 710 909 901 262 1553
Fragments arranged by size:
1553 926 909 901 710 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 2394 2897 513
Size: 552 939 285 105 503 3074
Fragments arranged by size:
3074 939 552 503 285 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2211 2687 3485 3502 3655 3840 4285
Size: 1255 631 476 798 17 153 185 445
Cuts at: 4285 325
Size: 1498
Fragments arranged by size:
1498 1255 798 631 476 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2256 3704
Size: 274 80 33 51 1443 141 138 1448
Cuts at: 3704 5416 96
Size: 1712 138
Fragments arranged by size:
1712 1448 1443 274 141 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2631 2663 5418 5450 2631
Size: 32 2755 32 2639
Fragments arranged by size:
2755 2639 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2548 3266 276
Size: 53 83 186 936 1014 718 2468
Fragments arranged by size:
2468 1014 936 718 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI