Restriction map for
pFN21AB9780
(Circular) (Six-base) MAPSORT of: pFN21AB9780.seq Check: 221 from: 1 to: 4909
pFN21AB9780 4909 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2719 278
Size: 53 83 186 936 1183 2468
Fragments arranged by size:
2468 1183 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2252 1937
Size: 315 4594
Acc65I G'GTAC_C
Cuts at: 2230 2230
Size: 4909
AclI AA'CG_TT
Cuts at: 1563 3036 3409 1563
Size: 1473 373 3063
Fragments arranged by size:
3063 1473 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2972 4356 4624 1632
Size: 1340 1384 268 1917
Fragments arranged by size:
1917 1384 1340 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4712
AflIII A'CryG_T
Cuts at: 1203 3808 4785 1203
Size: 2605 977 1327
Fragments arranged by size:
2605 1327 977
AgeI A'CCGG_T
Cuts at: 3740 3740
Size: 4909
AhdI GACnn_n'nnGTC
Cuts at: 3638 3638
Size: 4909
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4909
AlwNI CAG_nnn'CTG
Cuts at: 2652 4224 2652
Size: 1572 3337
ApaLI G'TGCA_C
Cuts at: 2966 4122 2966
Size: 1156 3753
ApoI r'AATT_y
Cuts at: 1493 2218 2444 2596 4705 1493
Size: 725 226 152 2109 1697
Fragments arranged by size:
2109 1697 725 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3463 160
Size: 872 2431 1606
Fragments arranged by size:
2431 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4909
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 1870
Size: 73 291 4545
Fragments arranged by size:
4545 291 73
BamHI G'GATC_C
Cuts at: 2239 2606 2239
Size: 367 4542
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2230 3685 618
Size: 325 217 627 443 1455 1842
Fragments arranged by size:
1842 1455 627 443 325 217
BanII G_rGCy'C
Cuts at: 1391 2228 1391
Size: 837 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3100 3134 1903
Size: 34 1163 34 3678
Fragments arranged by size:
3678 1163 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2801 4011 2801
Size: 1210 3699
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4909
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3520 136
Size: 107 122 71 631 605 1848 1525
Fragments arranged by size:
1848 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4904 4904
Size: 4909
BlpI GC'TnA_GC
Cuts at: 2326 2326
Size: 4909
Bme1580I G_kGCm'C
Cuts at: 1790 2970 4126 1790
Size: 1180 1156 2573
Fragments arranged by size:
2573 1180 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2640 3598 442
Size: 416 53 83 399 24 1223 958 1753
Fragments arranged by size:
1753 1223 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3569 1239
Size: 2330 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3034 3899 4197 3034
Size: 865 298 3746
Fragments arranged by size:
3746 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2092 3572 882
Size: 617 593 1480 2219
Fragments arranged by size:
2219 1480 617 593
BsaAI yAC'GTr
Cuts at: 493 1204 2700 493
Size: 711 1496 2702
Fragments arranged by size:
2702 1496 711
BsaBI GATnn'nnATC
Cuts at: 4903 4903
Size: 4909
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2099 2716 3098 3733
Size: 53 83 186 936 566 617 382 635
Cuts at: 3733 275
Size: 1451
Fragments arranged by size:
1451 936 635 617 566 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3342 3740 4014 4161 4572 3342
Size: 398 274 147 411 3679
Fragments arranged by size:
3679 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4684
Fragments arranged by size:
4684 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4909
BseYI C'CCAG_C
Cuts at: 4112 4608 4112
Size: 496 4413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2359 3120 4148 1540
Size: 275 186 358 761 1028 2301
Fragments arranged by size:
2301 1028 761 358 275 186
BsiHKAI G_wGCw'C
Cuts at: 2970 3055 4126 2970
Size: 85 1071 3753
Fragments arranged by size:
3753 1071 85
BsmI GAATG_Cn'
Cuts at: 2420 2513 2420
Size: 93 4816
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2970 3055 4126 1391
Size: 399 438 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 438 399 85
BspEI T'CCGG_A
Cuts at: 4572 4572
Size: 4909
BspHI T'CATG_A
Cuts at: 2109 2798 4528 2109
Size: 689 1730 2490
Fragments arranged by size:
2490 1730 689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2264 844
Size: 1420 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2796 1394
Size: 429 973 3507
Fragments arranged by size:
3507 973 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3404 3578 65
Size: 1183 2156 174 1396
Fragments arranged by size:
2156 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3553 3740 1700
Size: 110 1743 187 2869
Fragments arranged by size:
2869 1743 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4909
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4909
BssSI C'ACGA_G
Cuts at: 2969 3981 2969
Size: 1012 3897
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4909
BstBI TT'CG_AA
Cuts at: 3713 3713
Size: 4909
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4909
BstYI r'GATC_y
Cuts at: 1972 2239 2606 2991 3008 4460 4546 4558 4904
Size: 267 367 385 17 1452 86 12 346
Cuts at: 4904 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 267 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4170
Fragments arranged by size:
4170 683 56
BtsI GCAGTG_nn'
Cuts at: 2425 3219 3239 2425
Size: 794 20 4095
Fragments arranged by size:
4095 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4874
DraI TTT'AAA
Cuts at: 2213 2213
Size: 4909
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3916 809
Size: 507 2600 1802
Fragments arranged by size:
2600 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2356 3245 4874
Size: 54 1425 50 275 18 526 889 1629
Cuts at: 4874 8
Size: 43
Fragments arranged by size:
1629 1425 889 526 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2356 1537
Size: 275 544 4090
Fragments arranged by size:
4090 544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2839 1353
Size: 1486 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3496 3870 4016 387
Size: 1064 138 1907 374 146 1280
Fragments arranged by size:
1907 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2972 3569 4356 4624 1239
Size: 393 1340 597 787 268 1524
Fragments arranged by size:
1524 1340 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2218 2596 2218
Size: 378 4531
FspI TGC'GCA
Cuts at: 3415 3415
Size: 4909
HaeII r_GCGC'y
Cuts at: 1678 4056 1678
Size: 2378 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3550 3582 3624 3656 1373
Size: 32 135 32 1978 32 42 32 2626
Fragments arranged by size:
2626 1978 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2253 2499 669
Size: 988 281 315 246 3079
Fragments arranged by size:
3079 988 315 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4909
HpaI GTT'AAC
Cuts at: 2499 2499
Size: 4909
KpnI G_GTAC'C
Cuts at: 2234 2234
Size: 4909
MfeI C'AATT_G
Cuts at: 2508 2508
Size: 4909
MluI A'CGCG_T
Cuts at: 4785 4785
Size: 4909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2134 4023 4207 1305
Size: 829 1889 184 2007
Fragments arranged by size:
2007 1889 829 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3087
Fragments arranged by size:
3087 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 3226 3385 518
Size: 648 1701 359 159 2042
Fragments arranged by size:
2042 1701 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2325 3002 4150 4395 1606
Size: 719 677 1148 245 2120
Fragments arranged by size:
2120 1148 719 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4357
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4909
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4909
NotI GC'GGCC_GC
Cuts at: 2356 2356
Size: 4909
NspI r_CATG'y
Cuts at: 1132 1229 2132 2267 3812 1132
Size: 97 903 135 1545 2229
Fragments arranged by size:
2229 1545 903 135 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4909
PciI A'CATG_T
Cuts at: 3808 3808
Size: 4909
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4909
PmeI GTTT'AAAC
Cuts at: 2213 2213
Size: 4909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2978 3010 4491 4523 2978
Size: 32 1481 32 3364
Fragments arranged by size:
3364 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4909
PsiI TTA'TAA
Cuts at: 2479 2479
Size: 4909
PstI C_TGCA'G
Cuts at: 830 2261 830
Size: 1431 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4909
SalI G'TCGA_C
Cuts at: 1936 2251 1936
Size: 315 4594
SbfI CC_TGCA'GG
Cuts at: 2261 2261
Size: 4909
ScaI AGT'ACT
Cuts at: 3157 4869 3157
Size: 1712 3197
SfcI C'TryA_G
Cuts at: 826 1045 2257 3392 4073 4264 826
Size: 219 1212 1135 681 191 1471
Fragments arranged by size:
1471 1212 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4909
SmaI CCC'GGG
Cuts at: 1872 2236 1872
Size: 364 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3013 3914 4176 820
Size: 197 926 1070 901 262 1553
Fragments arranged by size:
1553 1070 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2700 493
Size: 2207 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4909
SphI G_CATG'C
Cuts at: 1132 2267 1132
Size: 1135 3774
SspI AAT'ATT
Cuts at: 5 52 2833 5
Size: 47 2781 2081
Fragments arranged by size:
2781 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4909
StyI C'CwwG_G
Cuts at: 513 1065 2348 513
Size: 552 1283 3074
Fragments arranged by size:
3074 1283 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2936 2953 3106 3291 3736 325
Size: 1255 1356 17 153 185 445 1498
Fragments arranged by size:
1498 1356 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3155 4867 96
Size: 274 80 33 51 1443 1178 1712 138
Fragments arranged by size:
1712 1443 1178 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4869 4901 4869
Size: 32 4877
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4909
XbaI T'CTAG_A
Cuts at: 2245 2245
Size: 4909
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4909
XmaI C'CCGG_G
Cuts at: 1870 2234 1870
Size: 364 4545
XmnI GAAnn'nnTTC
Cuts at: 3038 3038
Size: 4909
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2717 276
Size: 53 83 186 936 1183 2468
Fragments arranged by size:
2468 1183 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI