Restriction map for
pFN21AB9779
(Circular) (Six-base) MAPSORT of: pFN21AB9779.seq Check: 9047 from: 1 to: 7366
pFN21AB9779 7366 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4437 4437
Size: 7366
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5176 278
Size: 53 83 186 936 3640 2468
Fragments arranged by size:
3640 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4709 1937
Size: 2772 4594
Acc65I G'GTAC_C
Cuts at: 4687 4687
Size: 7366
AclI AA'CG_TT
Cuts at: 1563 5493 5866 1563
Size: 3930 373 3063
Fragments arranged by size:
3930 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3342 4098 5429 6813 7081 1632
Size: 1710 756 1331 1384 268 1917
Fragments arranged by size:
1917 1710 1384 1331 756 268
AflII C'TTAA_G
Cuts at: 820 1017 2066 820
Size: 197 1049 6120
Fragments arranged by size:
6120 1049 197
AflIII A'CryG_T
Cuts at: 1203 6265 7242 1203
Size: 5062 977 1327
Fragments arranged by size:
5062 1327 977
AgeI A'CCGG_T
Cuts at: 6197 6197
Size: 7366
AhdI GACnn_n'nnGTC
Cuts at: 6095 6095
Size: 7366
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7366
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4448 4480 4448
Size: 32 7334
AlwNI CAG_nnn'CTG
Cuts at: 2366 4070 4588 5109 6681 2366
Size: 1704 518 521 1572 3051
Fragments arranged by size:
3051 1704 1572 521 518
ApaLI G'TGCA_C
Cuts at: 5423 6579 5423
Size: 1156 6210
ApoI r'AATT_y
Cuts at: 1493 2663 2794 2999 3223 3298 3310 3754 4538
Size: 1170 131 205 224 75 12 444 784
Cuts at: 4538 4675 4901 5053 7162 1493
Size: 137 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 784 444 226 224 205
152 137 131 75 12
AseI AT'TA_AT
Cuts at: 160 1032 5920 160
Size: 872 4888 1606
Fragments arranged by size:
4888 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7366
AvaI C'yCGr_G
Cuts at: 1870 1943 4691 1870
Size: 73 2748 4545
Fragments arranged by size:
4545 2748 73
BamHI G'GATC_C
Cuts at: 4309 4696 5063 4309
Size: 387 367 6612
Fragments arranged by size:
6612 387 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4236 4687 6142 618
Size: 325 217 627 2449 451 1455 1842
Fragments arranged by size:
2449 1842 1455 627 451 325 217
BanII G_rGCy'C
Cuts at: 1391 4685 1391
Size: 3294 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 928
Size: 971 231 6164
Fragments arranged by size:
6164 971 231
BbvCI CC'TCA_GC
Cuts at: 4161 4161
Size: 7366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5557 5591 1903
Size: 34 3620 34 3678
Fragments arranged by size:
3678 3620 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5258 6468 5258
Size: 1210 6156
BclI T'GATC_A
Cuts at: 1548 4206 1548
Size: 2658 4708
BfrBI ATG'CAT
Cuts at: 2425 2425
Size: 7366
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 3044 5977
Size: 107 122 71 631 605 649 723 2933
Cuts at: 5977 136
Size: 1525
Fragments arranged by size:
2933 1525 723 649 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2944 3424 7361 2944
Size: 480 3937 2949
Fragments arranged by size:
3937 2949 480
BlpI GC'TnA_GC
Cuts at: 4783 4783
Size: 7366
Bme1580I G_kGCm'C
Cuts at: 1790 3850 4373 5427 6583 1790
Size: 2060 523 1054 1156 2573
Fragments arranged by size:
2573 2060 1156 1054 523
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4645 5097 6055
Size: 416 53 83 399 24 3228 452 958
Cuts at: 6055 442
Size: 1753
Fragments arranged by size:
3228 1753 958 452 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 7366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4678 6026 1239
Size: 3439 1348 2579
Fragments arranged by size:
3439 2579 1348
Bpu10I CC'TnA_GC
Cuts at: 4161 4161
Size: 7366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3015 5491 6356 6654 3015
Size: 2476 865 298 3727
Fragments arranged by size:
3727 2476 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6029 882
Size: 617 4530 2219
Fragments arranged by size:
4530 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5157 493
Size: 711 3953 2702
Fragments arranged by size:
3953 2702 711
BsaBI GATnn'nnATC
Cuts at: 7360 7360
Size: 7366
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5173 5555 6190 275
Size: 53 83 186 936 3640 382 635 1451
Fragments arranged by size:
3640 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3820 5799 6197 6471 6618 7029 3820
Size: 1979 398 274 147 411 4157
Fragments arranged by size:
4157 1979 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2464 2494 1178
Size: 30 165 30 1061 30 6050
Fragments arranged by size:
6050 1061 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3363 1186
Size: 2177 5189
BseYI C'CCAG_C
Cuts at: 6569 7065 6569
Size: 496 6870
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3618 3843 1163
Size: 573 1882 225 4686
Fragments arranged by size:
4686 1882 573 225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4816 5577 6605 1540
Size: 275 186 2815 761 1028 2301
Fragments arranged by size:
2815 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5427 5512 6583 5427
Size: 85 1071 6210
Fragments arranged by size:
6210 1071 85
BsmI GAATG_Cn'
Cuts at: 2317 4101 4877 4970 2317
Size: 1784 776 93 4713
Fragments arranged by size:
4713 1784 776 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3850 4373 4685 5427 5512 6583 1391
Size: 399 2060 523 312 742 85 1071 2174
Fragments arranged by size:
2174 2060 1071 742 523 399 312 85
BspEI T'CCGG_A
Cuts at: 3820 7029 3820
Size: 3209 4157
BspHI T'CATG_A
Cuts at: 5255 6985 5255
Size: 1730 5636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 4437 4721 844
Size: 1466 2127 284 3489
Fragments arranged by size:
3489 2127 1466 284
BsrBI CCG'CTC
Cuts at: 1394 1823 5253 1394
Size: 429 3430 3507
Fragments arranged by size:
3507 3430 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3728 5861 6035 65
Size: 1183 2480 2133 174 1396
Fragments arranged by size:
2480 2133 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6010 6197 1700
Size: 110 4200 187 2869
Fragments arranged by size:
4200 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7366
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7366
BssSI C'ACGA_G
Cuts at: 2878 5426 6438 2878
Size: 2548 1012 3806
Fragments arranged by size:
3806 2548 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4579 794
Size: 3785 3581
BstBI TT'CG_AA
Cuts at: 6170 6170
Size: 7366
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7366
BstYI r'GATC_y
Cuts at: 1972 2944 3022 3424 3532 4309 4696 5063 5448
Size: 972 78 402 108 777 387 367 385
Cuts at: 5448 5465 6917 7003 7015 7361 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 972 777 402 387 385 367
346 108 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5875
Fragments arranged by size:
5875 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6627
Fragments arranged by size:
6627 683 56
BtsI GCAGTG_nn'
Cuts at: 2367 4629 4882 5676 5696 2367
Size: 2262 253 794 20 4037
Fragments arranged by size:
4037 2262 794 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7331
DraI TTT'AAA
Cuts at: 3841 4670 3841
Size: 829 6537
DraIII CAC_nnn'GTG
Cuts at: 2566 4133 4379 2566
Size: 1567 246 5553
Fragments arranged by size:
5553 1567 246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2435 6373 809
Size: 507 1119 3938 1802
Fragments arranged by size:
3938 1802 1119 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4813 5702 7331
Size: 54 1425 50 275 18 2983 889 1629
Cuts at: 7331 8
Size: 43
Fragments arranged by size:
2983 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4813 1537
Size: 275 3001 4090
Fragments arranged by size:
4090 3001 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 3370 4280 4326 4557 5296 1353
Size: 731 1286 910 46 231 739 3423
Fragments arranged by size:
3423 1286 910 739 731 231 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5953 6327 6473 387
Size: 1064 138 4364 374 146 1280
Fragments arranged by size:
4364 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3342 4098 4678 5429 6026 6813 7081
Size: 393 1710 756 580 751 597 787 268
Cuts at: 7081 1239
Size: 1524
Fragments arranged by size:
1710 1524 787 756 751 597 580 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2370 2370
Size: 7366
EcoRI G'AATT_C
Cuts at: 4675 5053 4675
Size: 378 6988
EcoRV GAT'ATC
Cuts at: 2053 3444 2053
Size: 1391 5975
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2862 3956 2862
Size: 1094 6272
FspI TGC'GCA
Cuts at: 5872 5872
Size: 7366
HaeII r_GCGC'y
Cuts at: 1678 6513 1678
Size: 4835 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2509 2541 2717 2749 3089
Size: 32 135 32 937 32 176 32 340
Cuts at: 3089 3121 6007 6039 6081 6113 1373
Size: 32 2886 32 42 32 2626
Fragments arranged by size:
2886 2626 937 340 176 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4710 4956 669
Size: 988 281 2772 246 3079
Fragments arranged by size:
3079 2772 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2077 2819 748
Size: 1329 742 5295
Fragments arranged by size:
5295 1329 742
HpaI GTT'AAC
Cuts at: 4956 4956
Size: 7366
KpnI G_GTAC'C
Cuts at: 4691 4691
Size: 7366
MfeI C'AATT_G
Cuts at: 3197 4965 3197
Size: 1768 5598
MluI A'CGCG_T
Cuts at: 7242 7242
Size: 7366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4207 4536 6480 6664 1305
Size: 2902 329 1944 184 2007
Fragments arranged by size:
2902 2007 1944 329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5544
Fragments arranged by size:
5544 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3918 5324 5683 5842 518
Size: 648 2752 1406 359 159 2042
Fragments arranged by size:
2752 2042 1406 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4782 5459 6607 6852 1606
Size: 3176 677 1148 245 2120
Fragments arranged by size:
3176 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5875
Fragments arranged by size:
5875 939 552
NdeI CA'TA_TG
Cuts at: 387 2333 2932 387
Size: 1946 599 4821
Fragments arranged by size:
4821 1946 599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7366
NotI GC'GGCC_GC
Cuts at: 4813 4813
Size: 7366
NsiI A_TGCA'T
Cuts at: 2427 2427
Size: 7366
NspI r_CATG'y
Cuts at: 1132 1229 2166 2410 4582 4724 6269 1132
Size: 97 937 244 2172 142 1545 2229
Fragments arranged by size:
2229 2172 1545 937 244 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7366
PciI A'CATG_T
Cuts at: 6265 6265
Size: 7366
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7366
PfoI T'CCnGG_A
Cuts at: 2118 2350 2118
Size: 232 7134
PmeI GTTT'AAAC
Cuts at: 4670 4670
Size: 7366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3411 3443 5435 5467 6948 6980 3411
Size: 32 1992 32 1481 32 3797
Fragments arranged by size:
3797 1992 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7366
PsiI TTA'TAA
Cuts at: 2109 3341 4014 4936 2109
Size: 1232 673 922 4539
Fragments arranged by size:
4539 1232 922 673
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4379 4411 4379
Size: 32 7334
PstI C_TGCA'G
Cuts at: 830 3319 4624 4718 830
Size: 2489 1305 94 3478
Fragments arranged by size:
3478 2489 1305 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7366
SalI G'TCGA_C
Cuts at: 1936 4708 1936
Size: 2772 4594
SbfI CC_TGCA'GG
Cuts at: 4718 4718
Size: 7366
ScaI AGT'ACT
Cuts at: 4655 5614 7326 4655
Size: 959 1712 4695
Fragments arranged by size:
4695 1712 959
SfcI C'TryA_G
Cuts at: 826 1045 2035 2367 3315 3722 4620 4714 5849
Size: 219 990 332 948 407 898 94 1135
Cuts at: 5849 6530 6721 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 990 948 898 681 407 332
219 191 94
SfiI GGCCn_nnn'nGGCC
Cuts at: 3044 3044
Size: 7366
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7366
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7366
SmaI CCC'GGG
Cuts at: 1872 4693 1872
Size: 2821 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2066 3030 5470 6371 6633 820
Size: 197 926 123 964 2440 901 262 1553
Fragments arranged by size:
2440 1553 964 926 901 262 197 123
SnaBI TAC'GTA
Cuts at: 493 5157 493
Size: 4664 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7366
SphI G_CATG'C
Cuts at: 1132 4724 1132
Size: 3592 3774
SspI AAT'ATT
Cuts at: 5 52 2896 5290 5
Size: 47 2844 2394 2081
Fragments arranged by size:
2844 2394 2081 47
StuI AGG'CCT
Cuts at: 1517 3047 1517
Size: 1530 5836
StyI C'CwwG_G
Cuts at: 513 1065 2004 2416 3048 3093 4805 513
Size: 552 939 412 632 45 1712 3074
Fragments arranged by size:
3074 1712 939 632 552 412 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5393 5410 5563 5748 6193 325
Size: 1255 3813 17 153 185 445 1498
Fragments arranged by size:
3813 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2249 3552 3679
Size: 274 80 33 51 1443 272 1303 127
Cuts at: 3679 4031 4653 5612 7324 96
Size: 352 622 959 1712 138
Fragments arranged by size:
1712 1443 1303 959 622 352 274 272
138 127 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2346 2378 2464 2496 2550 2582 4188 4220 7326
Size: 32 86 32 54 32 1606 32 3106
Cuts at: 7326 7358 2346
Size: 32 2354
Fragments arranged by size:
3106 2354 1606 86 54 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7366
XbaI T'CTAG_A
Cuts at: 4702 4702
Size: 7366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3046 4286 3046
Size: 1240 6126
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7366
XmaI C'CCGG_G
Cuts at: 1870 4691 1870
Size: 2821 4545
XmnI GAAnn'nnTTC
Cuts at: 3310 4438 4635 5495 3310
Size: 1128 197 860 5181
Fragments arranged by size:
5181 1128 860 197
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5174 276
Size: 53 83 186 936 3640 2468
Fragments arranged by size:
3640 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfoI SrfI SwaI