Restriction map for
pFN21AB9775
(Circular) (Six-base) MAPSORT of: pFN21AB9775.seq Check: 1293 from: 1 to: 6739
pFN21AB9775 6739 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3993 3993
Size: 6739
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2301 4549 278
Size: 53 83 186 936 765 2248 2468
Fragments arranged by size:
2468 2248 936 765 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 4082 1937
Size: 343 1802 4594
Fragments arranged by size:
4594 1802 343
Acc65I G'GTAC_C
Cuts at: 4060 4060
Size: 6739
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2280 2448 2516 3140 3742 4802 6186
Size: 596 52 168 68 624 602 1060 1384
Cuts at: 6186 6454 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1060 624 602 596 268 168
68 52
AfeI AGC'GCT
Cuts at: 2045 3536 2045
Size: 1491 5248
AflII C'TTAA_G
Cuts at: 820 1017 2794 820
Size: 197 1777 4765
Fragments arranged by size:
4765 1777 197
AflIII A'CryG_T
Cuts at: 1203 5638 6615 1203
Size: 4435 977 1327
Fragments arranged by size:
4435 1327 977
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 5468 5468
Size: 6739
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6739
AlwNI CAG_nnn'CTG
Cuts at: 2062 2359 2494 3028 3311 3349 3601 4482 6054
Size: 297 135 534 283 38 252 881 1572
Cuts at: 6054 2062
Size: 2747
Fragments arranged by size:
2747 1572 881 534 297 283 252 135
38
ApaLI G'TGCA_C
Cuts at: 4796 5952 4796
Size: 1156 5583
ApoI r'AATT_y
Cuts at: 1493 2420 2992 4048 4274 4426 6535 1493
Size: 927 572 1056 226 152 2109 1697
Fragments arranged by size:
2109 1697 1056 927 572 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5293 160
Size: 872 4261 1606
Fragments arranged by size:
4261 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 4064 1870
Size: 73 2121 4545
Fragments arranged by size:
4545 2121 73
BamHI G'GATC_C
Cuts at: 4069 4436 4069
Size: 367 6372
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2854 3389 3476 4060 5515
Size: 325 217 627 1067 535 87 584 1455
Cuts at: 5515 618
Size: 1842
Fragments arranged by size:
1842 1455 1067 627 584 535 325 217
87
BanII G_rGCy'C
Cuts at: 1391 3426 4058 1391
Size: 2035 632 4072
Fragments arranged by size:
4072 2035 632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3892 928
Size: 971 1993 3775
Fragments arranged by size:
3775 1993 971
BbvCI CC'TCA_GC
Cuts at: 3818 3818
Size: 6739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4930 4964 1903
Size: 34 2993 34 3678
Fragments arranged by size:
3678 2993 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5841 4631
Size: 1210 5529
BclI T'GATC_A
Cuts at: 1548 2730 1548
Size: 1182 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5350 136
Size: 107 122 71 631 605 337 3341 1525
Fragments arranged by size:
3341 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3006 6734 3006
Size: 3728 3011
BlpI GC'TnA_GC
Cuts at: 2616 3029 3396 4156 2616
Size: 413 367 760 5199
Fragments arranged by size:
5199 760 413 367
Bme1580I G_kGCm'C
Cuts at: 1790 3481 3547 4800 5956 1790
Size: 1691 66 1253 1156 2573
Fragments arranged by size:
2573 1691 1253 1156 66
BmgBI CAC'GTC
Cuts at: 1318 2863 2953 1318
Size: 1545 90 5104
Fragments arranged by size:
5104 1545 90
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3718 4470 5428
Size: 416 53 83 399 24 2301 752 958
Cuts at: 5428 442
Size: 1753
Fragments arranged by size:
2301 1753 958 752 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2324 2627 2813 2878 2948 3200 3215
Size: 918 167 303 186 65 70 252 15
Cuts at: 3215 3359 3428 3505 3578 5399 1239
Size: 144 69 77 73 1821 2579
Fragments arranged by size:
2579 1821 918 303 252 186 167 144
77 73 70 69 65 15
Bpu10I CC'TnA_GC
Cuts at: 3254 3350 3788 3818 3254
Size: 96 438 30 6175
Fragments arranged by size:
6175 438 96 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 3038 3581 3814 4864 5729 6027 2543
Size: 495 543 233 1050 865 298 3255
Fragments arranged by size:
3255 1050 865 543 495 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2783 5402 882
Size: 617 1284 2619 2219
Fragments arranged by size:
2619 2219 1284 617
BsaAI yAC'GTr
Cuts at: 493 1204 4530 493
Size: 711 3326 2702
Fragments arranged by size:
3326 2702 711
BsaBI GATnn'nnATC
Cuts at: 6733 6733
Size: 6739
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2298 3829 4546 4928
Size: 53 83 186 936 765 1531 717 382
Cuts at: 4928 5563 275
Size: 635 1451
Fragments arranged by size:
1531 1451 936 765 717 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5172 5570 5844 5991 6402 5172
Size: 398 274 147 411 5509
Fragments arranged by size:
5509 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2322 2352 3173 3203 3698
Size: 30 165 30 919 30 821 30 495
Cuts at: 3698 3728 3734 3764 1178
Size: 30 6 30 4153
Fragments arranged by size:
4153 919 821 495 165 30 30 30
30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3742 3746 1186
Size: 2556 4 4179
Fragments arranged by size:
4179 2556 4
BseYI C'CCAG_C
Cuts at: 3152 3582 5942 6438 3152
Size: 430 2360 496 3453
Fragments arranged by size:
3453 2360 496 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 2372 2975 3288 3872 3971 1163
Size: 573 609 27 603 313 584 99 3931
Fragments arranged by size:
3931 609 603 584 573 313 99 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3402 3912 4800 4885 5956 3402
Size: 510 888 85 1071 4185
Fragments arranged by size:
4185 1071 888 510 85
BsmI GAATG_Cn'
Cuts at: 4250 4343 4250
Size: 93 6646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3402 3426 3481 3547 3912 4058 4800
Size: 399 1612 24 55 66 365 146 742
Cuts at: 4800 4885 5956 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1612 1071 742 399 365 146 85
66 55 24
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 2733 4628 6358 2733
Size: 1895 1730 3114
Fragments arranged by size:
3114 1895 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2182 3466 3993 4027 4094 844
Size: 1338 1284 527 34 67 3489
Fragments arranged by size:
3489 1338 1284 527 67 34
BsrBI CCG'CTC
Cuts at: 1394 1823 2892 2926 3207 3258 3772 4626 1394
Size: 429 1069 34 281 51 514 854 3507
Fragments arranged by size:
3507 1069 854 514 429 281 51 34
BsrDI GCAATG_nn'
Cuts at: 65 1248 5234 5408 65
Size: 1183 3986 174 1396
Fragments arranged by size:
3986 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 5383 5570 1700
Size: 110 293 3280 187 2869
Fragments arranged by size:
3280 2869 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6739
BssHII G'CGCG_C
Cuts at: 1925 3625 3854 1925
Size: 1700 229 4810
Fragments arranged by size:
4810 1700 229
BssSI C'ACGA_G
Cuts at: 3998 4799 5811 3998
Size: 801 1012 4926
Fragments arranged by size:
4926 1012 801
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2359 794
Size: 1565 5174
BstBI TT'CG_AA
Cuts at: 5543 5543
Size: 6739
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6739
BstXI CCAn_nnnn'nTGG
Cuts at: 3178 3850 3178
Size: 672 6067
BstYI r'GATC_y
Cuts at: 1972 2305 2323 2844 3006 3414 4069 4436 4821
Size: 333 18 521 162 408 655 367 385
Cuts at: 4821 4838 6290 6376 6388 6734 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 655 521 408 385 367 346
333 162 86 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3126 513
Size: 552 939 1122 4126
Fragments arranged by size:
4126 1122 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6000
Fragments arranged by size:
6000 683 56
BtsI GCAGTG_nn'
Cuts at: 3438 3503 3700 3889 4255 5049 5069 3438
Size: 65 197 189 366 794 20 5108
Fragments arranged by size:
5108 794 366 197 189 65 20
ClaI AT'CG_AT
Cuts at: 1554 2024 1554
Size: 470 6269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 4043 4043
Size: 6739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2587 5746 809
Size: 507 1271 3159 1802
Fragments arranged by size:
3159 1802 1271 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2814 2913
Size: 54 1425 50 275 18 180 804 99
Cuts at: 2913 2997 3168 3300 3679 4186 5075 6704 8
Size: 84 171 132 379 507 889 1629 43
Fragments arranged by size:
1629 1425 889 804 507 379 275 180
171 132 99 84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 3087 4669 1353
Size: 851 883 1582 3423
Fragments arranged by size:
3423 1582 883 851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2801 5326 5700 5846 387
Size: 1064 138 1212 2525 374 146 1280
Fragments arranged by size:
2525 1280 1212 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2228 2280 2324 2448 2516 2627
Size: 393 525 71 52 44 124 68 111
Cuts at: 2627 2813 2878 2948 3140 3200 3215 3359 3428
Size: 186 65 70 192 60 15 144 69
Cuts at: 3428 3505 3578 3742 4802 5399 6186 6454 1239
Size: 77 73 164 1060 597 787 268 1524
Fragments arranged by size:
1524 1060 787 597 525 393 268 192
186 164 144 124 111 77 73 71
70 69 68 65 60 52 44 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3341 3485 3341
Size: 144 6595
EcoO109I rG'GnC_Cy
Cuts at: 3346 3598 3346
Size: 252 6487
EcoRI G'AATT_C
Cuts at: 2992 4048 4426 2992
Size: 1056 378 5305
Fragments arranged by size:
5305 1056 378
FspI TGC'GCA
Cuts at: 2186 3236 5245 2186
Size: 1050 2009 3680
Fragments arranged by size:
3680 2009 1050
HaeII r_GCGC'y
Cuts at: 1678 2047 2201 2359 3036 3153 3538 3739 5886
Size: 369 154 158 677 117 385 201 2147
Cuts at: 5886 1678
Size: 2531
Fragments arranged by size:
2531 2147 677 385 369 201 158 154
117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2322 2354 3312 3344 3734
Size: 32 135 32 750 32 958 32 390
Cuts at: 3734 3766 3781 3813 5380 5412 5454 5486 1373
Size: 32 15 32 1567 32 42 32 2626
Fragments arranged by size:
2626 1567 958 750 390 135 42 32
32 32 32 32 32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4083 4329 669
Size: 988 281 2145 246 3079
Fragments arranged by size:
3079 2145 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6739
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KpnI G_GTAC'C
Cuts at: 4064 4064
Size: 6739
MfeI C'AATT_G
Cuts at: 4338 4338
Size: 6739
MluI A'CGCG_T
Cuts at: 6615 6615
Size: 6739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3267 3867 5853 6037 1305
Size: 1962 600 1986 184 2007
Fragments arranged by size:
2007 1986 1962 600 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3302 10
Size: 54 1425 343 1470 3447
Fragments arranged by size:
3447 1470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 4002 4697 5056 5215 518
Size: 648 1713 1123 695 359 159 2042
Fragments arranged by size:
2042 1713 1123 695 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2203 2833 2941 3028 3373 3395 3474
Size: 456 141 630 108 87 345 22 79
Cuts at: 3474 3690 4155 4832 5980 6225 1606
Size: 216 465 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 465 456 345 245
216 141 108 87 79 22
NcoI C'CATG_G
Cuts at: 513 1065 2004 3126 513
Size: 552 939 1122 4126
Fragments arranged by size:
4126 1122 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6739
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6739
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 4097 5642 1132
Size: 97 2868 1545 2229
Fragments arranged by size:
2868 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3801 1348
Size: 2453 4286
PciI A'CATG_T
Cuts at: 5638 5638
Size: 6739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6739
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6321 6353 4808
Size: 32 1481 32 5194
Fragments arranged by size:
5194 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3346 3346
Size: 6739
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6739
PsiI TTA'TAA
Cuts at: 4309 4309
Size: 6739
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2523 2555 2523
Size: 32 6707
PstI C_TGCA'G
Cuts at: 830 2067 2577 3216 3463 3606 3669 4091 830
Size: 1237 510 639 247 143 63 422 3478
Fragments arranged by size:
3478 1237 639 510 422 247 143 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 2062 2833 2941 3028 2062
Size: 771 108 87 5773
Fragments arranged by size:
5773 771 108 87
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 4987 6699 4987
Size: 1712 5027
SfcI C'TryA_G
Cuts at: 826 1045 2063 2573 3212 3459 3602 3665 4087
Size: 219 1018 510 639 247 143 63 422
Cuts at: 4087 5222 5903 6094 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1018 681 639 510 422 247
219 191 143 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2522 2794 3017 3560 3793 4843
Size: 197 926 579 272 223 543 233 1050
Cuts at: 4843 5744 6006 820
Size: 901 262 1553
Fragments arranged by size:
1553 1050 926 901 579 543 272 262
233 223 197
SnaBI TAC'GTA
Cuts at: 493 4530 493
Size: 4037 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 4097 1132
Size: 2965 3774
SspI AAT'ATT
Cuts at: 5 52 2036 4663 5
Size: 47 1984 2627 2081
Fragments arranged by size:
2627 2081 1984 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6739
StyI C'CwwG_G
Cuts at: 513 1065 2004 2218 2919 3126 4178 513
Size: 552 939 214 701 207 1052 3074
Fragments arranged by size:
3074 1052 939 701 552 214 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4766 4783 4936 5121 5566 325
Size: 1255 3186 17 153 185 445 1498
Fragments arranged by size:
3186 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4985 6697 96
Size: 274 80 33 51 1443 3008 1712 138
Fragments arranged by size:
3008 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6699 6731 6699
Size: 32 6707
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6739
XbaI T'CTAG_A
Cuts at: 4075 4075
Size: 6739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2602 3820 2602
Size: 1218 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6739
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 2213 4868 2213
Size: 2655 4084
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2299 4547 276
Size: 53 83 186 936 765 2248 2468
Fragments arranged by size:
2468 2248 936 765 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI