Restriction map for
pFN21AB9773
(Circular) (Six-base) MAPSORT of: pFN21AB9773.seq Check: 8764 from: 1 to: 6628
pFN21AB9773 6628 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2305 3796 2305
Size: 1491 5137
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3971 1937
Size: 2034 4594
Acc65I G'GTAC_C
Cuts at: 3949 3949
Size: 6628
AclI AA'CG_TT
Cuts at: 1563 4755 5128 1563
Size: 3192 373 3063
Fragments arranged by size:
3192 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4691 6075 6343 1632
Size: 3059 1384 268 1917
Fragments arranged by size:
3059 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 2622 2886 5527 6504 1203
Size: 1419 264 2641 977 1327
Fragments arranged by size:
2641 1419 1327 977 264
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 5357 5357
Size: 6628
AleI CACnn'nnGTG
Cuts at: 1166 2688 1166
Size: 1522 5106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6628
AlwNI CAG_nnn'CTG
Cuts at: 3049 3175 4371 5943 3049
Size: 126 1196 1572 3734
Fragments arranged by size:
3734 1572 1196 126
ApaLI G'TGCA_C
Cuts at: 4685 5841 4685
Size: 1156 5472
ApoI r'AATT_y
Cuts at: 1493 3937 4163 4315 6424 1493
Size: 2444 226 152 2109 1697
Fragments arranged by size:
2444 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5182 160
Size: 872 4150 1606
Fragments arranged by size:
4150 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 2351 3311 3854 3953 1870
Size: 73 408 960 543 99 4545
Fragments arranged by size:
4545 960 543 408 99 73
BamHI G'GATC_C
Cuts at: 3958 4325 3958
Size: 367 6261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2612 3842 3949 5404
Size: 325 217 627 252 573 1230 107 1455
Cuts at: 5404 618
Size: 1842
Fragments arranged by size:
1842 1455 1230 627 573 325 252 217
107
BanII G_rGCy'C
Cuts at: 1391 2505 2532 2952 3023 3678 3877 3947 1391
Size: 1114 27 420 71 655 199 70 4072
Fragments arranged by size:
4072 1114 655 420 199 71 70 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3428 3462 4819 4853 1903
Size: 34 1491 34 1357 34 3678
Fragments arranged by size:
3678 1491 1357 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4520 5730 4520
Size: 1210 5418
BclI T'GATC_A
Cuts at: 1548 2286 2433 3243 1548
Size: 738 147 810 4933
Fragments arranged by size:
4933 810 738 147
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3119 5239 136
Size: 107 122 71 631 605 1447 2120 1525
Fragments arranged by size:
2120 1525 1447 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2358 3282 6623 2358
Size: 924 3341 2363
Fragments arranged by size:
3341 2363 924
BlpI GC'TnA_GC
Cuts at: 3386 4045 3386
Size: 659 5969
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2615 3420 4689 5845 1790
Size: 252 573 805 1269 1156 2573
Fragments arranged by size:
2573 1269 1156 805 573 252
BmgBI CAC'GTC
Cuts at: 1318 2623 2889 3190 1318
Size: 1305 266 301 4756
Fragments arranged by size:
4756 1305 301 266
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4359 5317 442
Size: 416 53 83 399 24 2942 958 1753
Fragments arranged by size:
2942 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6628
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2875 2938 3754 2377
Size: 498 63 816 5251
Fragments arranged by size:
5251 816 498 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2870 2966 3233 3650 3821 5288 1239
Size: 1631 96 267 417 171 1467 2579
Fragments arranged by size:
2579 1631 1467 417 267 171 96
Bpu10I CC'TnA_GC
Cuts at: 3533 3876 3533
Size: 343 6285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3662 4753 5618 5916 3662
Size: 1091 865 298 4374
Fragments arranged by size:
4374 1091 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5291 882
Size: 617 3792 2219
Fragments arranged by size:
3792 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2128 4419 493
Size: 711 924 2291 2702
Fragments arranged by size:
2702 2291 924 711
BsaBI GATnn'nnATC
Cuts at: 2027 3509 6622 2027
Size: 1482 3113 2033
Fragments arranged by size:
3113 2033 1482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2973 3750 4435 4817
Size: 53 83 186 936 1440 777 685 382
Cuts at: 4817 5452 275
Size: 635 1451
Fragments arranged by size:
1451 1440 936 777 685 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5061 5459 5733 5880 6291 5061
Size: 398 274 147 411 5398
Fragments arranged by size:
5398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 3794 3824 1178
Size: 30 165 30 945 30 1416 30 3982
Fragments arranged by size:
3982 1416 945 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2747 2927 3557 3932 1186
Size: 1183 378 180 630 375 3882
Fragments arranged by size:
3882 1183 630 378 375 180
BseYI C'CCAG_C
Cuts at: 2120 3205 5831 6327 2120
Size: 1085 2626 496 2421
Fragments arranged by size:
2626 2421 1085 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 2333 3062 3149 3188 3581 3617
Size: 573 537 60 729 87 39 393 36
Cuts at: 3617 1163
Size: 4174
Fragments arranged by size:
4174 729 573 537 393 87 60 39
36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 2230 2538 2640 2673 3222
Size: 275 186 24 205 308 102 33 549
Cuts at: 3222 4078 4839 5867 1540
Size: 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 549 308 275 205
186 102 33 24
BsiHKAI G_wGCw'C
Cuts at: 2592 2924 3488 3786 4689 4774 5845 2592
Size: 332 564 298 903 85 1071 3375
Fragments arranged by size:
3375 1071 903 564 332 298 85
BsmI GAATG_Cn'
Cuts at: 2342 4139 4232 2342
Size: 1797 93 4738
Fragments arranged by size:
4738 1797 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3209 3209
Size: 6628
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2505 2532 2592 2615 2924 2952
Size: 399 252 463 27 60 23 309 28
Cuts at: 2952 3023 3420 3488 3678 3786 3877 3947 4689
Size: 71 397 68 190 108 91 70 742
Cuts at: 4689 4774 5845 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 463 399 397 309 252
190 108 91 85 71 70 68 60
28 27 23
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 4517 6247 4517
Size: 1730 4898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2305 3183 3796 3983 844
Size: 1461 878 613 187 3489
Fragments arranged by size:
3489 1461 878 613 187
BsrBI CCG'CTC
Cuts at: 1394 1823 2149 3390 4515 1394
Size: 429 326 1241 1125 3507
Fragments arranged by size:
3507 1241 1125 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 5123 5297 65
Size: 1183 3875 174 1396
Fragments arranged by size:
3875 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2443 3488 3845 5272 5459 1700
Size: 110 633 1045 357 1427 187 2869
Fragments arranged by size:
2869 1427 1045 633 357 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6628
BssSI C'ACGA_G
Cuts at: 3739 4688 5700 3739
Size: 949 1012 4667
Fragments arranged by size:
4667 1012 949
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2802 3049 3175 794
Size: 2008 247 126 4247
Fragments arranged by size:
4247 2008 247 126
BstBI TT'CG_AA
Cuts at: 5432 5432
Size: 6628
BstEII G'GTnAC_C
Cuts at: 1184 2419 3430 1184
Size: 1235 1011 4382
Fragments arranged by size:
4382 1235 1011
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2690 3145 3760 2128
Size: 562 455 615 4996
Fragments arranged by size:
4996 615 562 455
BstYI r'GATC_y
Cuts at: 1972 2358 3282 3958 4325 4710 4727 6179 6265
Size: 386 924 676 367 385 17 1452 86
Cuts at: 6265 6277 6623 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 924 676 386 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2367 2532 2667 2952 3474
Size: 552 939 12 351 165 135 285 522
Cuts at: 3474 3660 513
Size: 186 3481
Fragments arranged by size:
3481 939 552 522 351 285 186 165
135 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2074 2530 487
Size: 683 56 848 456 4585
Fragments arranged by size:
4585 848 683 456 56
BtsI GCAGTG_nn'
Cuts at: 4144 4938 4958 4144
Size: 794 20 5814
Fragments arranged by size:
5814 794 20
ClaI AT'CG_AT
Cuts at: 1554 3444 1554
Size: 1890 4738
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6593
DraI TTT'AAA
Cuts at: 3932 3932
Size: 6628
DraIII CAC_nnn'GTG
Cuts at: 2728 2728
Size: 6628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2827 5635 809
Size: 507 1511 2808 1802
Fragments arranged by size:
2808 1802 1511 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2166 2227
Size: 54 1425 50 275 18 177 159 61
Cuts at: 2227 2535 2670 3135 3219 3471 3762 4075 4964
Size: 308 135 465 84 252 291 313 889
Cuts at: 4964 6593 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 465 313 308 291 275
252 177 159 135 84 61 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2227 2535 2670 3219 4075 1537
Size: 275 415 308 135 549 856 4090
Fragments arranged by size:
4090 856 549 415 308 275 135
EarI CTCTTCn'nnn_
Cuts at: 1353 3647 4558 1353
Size: 2294 911 3423
Fragments arranged by size:
3423 2294 911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3584 5215 5589 5735 387
Size: 1064 138 1995 1631 374 146 1280
Fragments arranged by size:
1995 1631 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2870 2966 3233 3650 3821 4691 5288
Size: 393 1238 96 267 417 171 870 597
Cuts at: 5288 6075 6343 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1238 870 787 597 417 393 268
267 171 96
EcoNI CCTnn'n_nnAGG
Cuts at: 2806 2867 3536 2806
Size: 61 669 5898
Fragments arranged by size:
5898 669 61
EcoO109I rG'GnC_Cy
Cuts at: 2820 3172 3881 3905 2820
Size: 352 709 24 5543
Fragments arranged by size:
5543 709 352 24
EcoRI G'AATT_C
Cuts at: 3937 4315 3937
Size: 378 6250
FspI TGC'GCA
Cuts at: 2795 5134 2795
Size: 2339 4289
HaeII r_GCGC'y
Cuts at: 1678 2186 2470 3379 3660 5775 1678
Size: 508 284 909 281 2115 2531
Fragments arranged by size:
2531 2115 909 508 284 281
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 3596 3628 5269
Size: 32 135 32 776 32 1216 32 1641
Cuts at: 5269 5301 5343 5375 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1641 1216 776 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3972 4218 669
Size: 988 281 2034 246 3079
Fragments arranged by size:
3079 2034 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6628
HpaI GTT'AAC
Cuts at: 4218 4218
Size: 6628
KpnI G_GTAC'C
Cuts at: 3953 3953
Size: 6628
MfeI C'AATT_G
Cuts at: 4227 4227
Size: 6628
MluI A'CGCG_T
Cuts at: 6504 6504
Size: 6628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2092 2238 2949 3324 5742 5926 1305
Size: 787 146 711 375 2418 184 2007
Fragments arranged by size:
2418 2007 787 711 375 184 146
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4629
Fragments arranged by size:
4629 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2688 3143 4586 4945 5104 518
Size: 648 1522 455 1443 359 159 2042
Fragments arranged by size:
2042 1522 1443 648 455 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 3828 4044 4721 5869 6114 1606
Size: 412 1810 216 677 1148 245 2120
Fragments arranged by size:
2120 1810 1148 677 412 245 216
NaeI GCC'GGC
Cuts at: 2445 2445
Size: 6628
NcoI C'CATG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 4774
Fragments arranged by size:
4774 939 552 363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6628
NgoMIV G'CCGG_C
Cuts at: 2443 2443
Size: 6628
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6628
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 3986 5531 1132
Size: 97 2757 1545 2229
Fragments arranged by size:
2757 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3408 1348
Size: 2060 4568
PciI A'CATG_T
Cuts at: 5527 5527
Size: 6628
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2728 1108
Size: 1620 5008
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4697 4729 6210 6242 4697
Size: 32 1481 32 5083
Fragments arranged by size:
5083 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2820 3172 3881 2820
Size: 352 709 5567
Fragments arranged by size:
5567 709 352
PshAI GACnn'nnGTC
Cuts at: 1613 3723 1613
Size: 2110 4518
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PstI C_TGCA'G
Cuts at: 830 3057 3980 830
Size: 2227 923 3478
Fragments arranged by size:
3478 2227 923
PvuI CG_AT'CG
Cuts at: 2001 2025 2640 2001
Size: 24 615 5989
Fragments arranged by size:
5989 615 24
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 6628
SalI G'TCGA_C
Cuts at: 1936 3970 1936
Size: 2034 4594
SanDI GG'GwC_CC
Cuts at: 3881 3881
Size: 6628
SapI GCTCTTCn'nnn_
Cuts at: 3647 3647
Size: 6628
SbfI CC_TGCA'GG
Cuts at: 3980 3980
Size: 6628
ScaI AGT'ACT
Cuts at: 4876 6588 4876
Size: 1712 4916
SexAI A'CCwGG_T
Cuts at: 2175 3003 2175
Size: 828 5800
SfcI C'TryA_G
Cuts at: 826 1045 3053 3976 5111 5792 5983 826
Size: 219 2008 923 1135 681 191 1471
Fragments arranged by size:
2008 1471 1135 923 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6628
SmaI CCC'GGG
Cuts at: 1872 3313 3856 3955 1872
Size: 1441 543 99 4545
Fragments arranged by size:
4545 1441 543 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3677 4732 5633 5895 820
Size: 197 926 1734 1055 901 262 1553
Fragments arranged by size:
1734 1553 1055 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4419 493
Size: 3926 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6628
SphI G_CATG'C
Cuts at: 1132 3986 1132
Size: 2854 3774
SspI AAT'ATT
Cuts at: 5 52 4552 5
Size: 47 4500 2081
Fragments arranged by size:
4500 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6628
StyI C'CwwG_G
Cuts at: 513 1065 2004 2307 2337 2367 2478 3036 3759
Size: 552 939 303 30 30 111 558 723
Cuts at: 3759 3885 4067 513
Size: 126 182 3074
Fragments arranged by size:
3074 939 723 558 552 303 182 126
111 30 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2678 2696 2852 4655 4672 4825 5010
Size: 1255 1098 18 156 1803 17 153 185
Cuts at: 5010 5455 325
Size: 445 1498
Fragments arranged by size:
1803 1498 1255 1098 445 185 156 153
18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3576 4874 6586
Size: 274 80 33 51 1443 1599 1298 1712
Cuts at: 6586 96
Size: 138
Fragments arranged by size:
1712 1599 1443 1298 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3794 3826 6588 6620 3794
Size: 32 2762 32 3802
Fragments arranged by size:
3802 2762 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2824 3005 1316
Size: 1508 181 4939
Fragments arranged by size:
4939 1508 181
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 3625 3845 2176
Size: 1449 220 4959
Fragments arranged by size:
4959 1449 220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6628
XmaI C'CCGG_G
Cuts at: 1870 3311 3854 3953 1870
Size: 1441 543 99 4545
Fragments arranged by size:
4545 1441 543 99
XmnI GAAnn'nnTTC
Cuts at: 2723 4757 2723
Size: 2034 4594
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SfiI SfoI
SrfI SwaI