Restriction map for
pFN21AB9772
(Circular) (Six-base) MAPSORT of: pFN21AB9772.seq Check: 455 from: 1 to: 6352
pFN21AB9772 6352 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2343 2611 3695 1937
Size: 406 268 1084 4594
Fragments arranged by size:
4594 1084 406 268
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 2386 4479 4852 1563
Size: 823 2093 373 3063
Fragments arranged by size:
3063 2093 823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2147 2401 2501 3490 3574 4415 5799
Size: 474 41 254 100 989 84 841 1384
Cuts at: 5799 6067 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 989 841 474 268 254 100
84 41
AflII C'TTAA_G
Cuts at: 820 1017 2282 2851 820
Size: 197 1265 569 4321
Fragments arranged by size:
4321 1265 569 197
AflIII A'CryG_T
Cuts at: 1203 2290 3398 3434 5251 6228 1203
Size: 1087 1108 36 1817 977 1327
Fragments arranged by size:
1817 1327 1108 1087 977 36
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6352
AlwNI CAG_nnn'CTG
Cuts at: 4095 5667 4095
Size: 1572 4780
ApaLI G'TGCA_C
Cuts at: 2452 4409 5565 2452
Size: 1957 1156 3239
Fragments arranged by size:
3239 1957 1156
ApoI r'AATT_y
Cuts at: 1493 2023 3007 3036 3288 3661 3887 4039 6148
Size: 530 984 29 252 373 226 152 2109
Cuts at: 6148 1493
Size: 1697
Fragments arranged by size:
2109 1697 984 530 373 252 226 152
29
AseI AT'TA_AT
Cuts at: 160 1032 2502 4906 160
Size: 872 1470 2404 1606
Fragments arranged by size:
2404 1606 1470 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3677 1870
Size: 73 1734 4545
Fragments arranged by size:
4545 1734 73
BamHI G'GATC_C
Cuts at: 2968 3456 3682 4049 2968
Size: 488 226 367 5271
Fragments arranged by size:
5271 488 367 226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3673 5128 618
Size: 325 217 627 1886 1455 1842
Fragments arranged by size:
1886 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3671 1391
Size: 2280 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4543 4577 1903
Size: 34 50 34 2522 34 3678
Fragments arranged by size:
3678 2522 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4244 5454 4244
Size: 1210 5142
BclI T'GATC_A
Cuts at: 1548 3025 1548
Size: 1477 4875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4963 136
Size: 107 122 71 631 605 3291 1525
Fragments arranged by size:
3291 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2924 6347 2924
Size: 3423 2929
BlpI GC'TnA_GC
Cuts at: 3769 3769
Size: 6352
Bme1580I G_kGCm'C
Cuts at: 1790 2456 4413 5569 1790
Size: 666 1957 1156 2573
Fragments arranged by size:
2573 1957 1156 666
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6352
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2052 4083 5041
Size: 416 53 83 399 24 635 2031 958
Cuts at: 5041 442
Size: 1753
Fragments arranged by size:
2031 1753 958 635 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2567 2206
Size: 361 5991
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2176 2248 2397 2730 2814 3234 3318
Size: 791 146 72 149 333 84 420 84
Cuts at: 3318 3334 3486 3570 5012 1239
Size: 16 152 84 1442 2579
Fragments arranged by size:
2579 1442 791 420 333 152 149 146
84 84 84 72 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4477 5342 5640 4477
Size: 865 298 5189
Fragments arranged by size:
5189 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5015 882
Size: 617 3516 2219
Fragments arranged by size:
3516 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 3435 4143 493
Size: 711 2195 36 708 2702
Fragments arranged by size:
2702 2195 711 708 36
BsaBI GATnn'nnATC
Cuts at: 6346 6346
Size: 6352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4159 4541 5176 275
Size: 53 83 186 936 2626 382 635 1451
Fragments arranged by size:
2626 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4785 5183 5457 5604 6015 4785
Size: 398 274 147 411 5122
Fragments arranged by size:
5122 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2217 2247 1178
Size: 30 165 30 814 30 5283
Fragments arranged by size:
5283 814 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6352
BseYI C'CCAG_C
Cuts at: 5555 6051 5555
Size: 496 5856
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2120 2342 1163
Size: 573 384 222 5173
Fragments arranged by size:
5173 573 384 222
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3802 4563 5591 1540
Size: 275 186 1801 761 1028 2301
Fragments arranged by size:
2301 1801 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2456 4413 4498 5569 2456
Size: 1957 85 1071 3239
Fragments arranged by size:
3239 1957 1071 85
BsmI GAATG_Cn'
Cuts at: 2900 3343 3863 3956 2900
Size: 443 520 93 5296
Fragments arranged by size:
5296 520 443 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2456 3671 4413 4498 5569 1391
Size: 399 666 1215 742 85 1071 2174
Fragments arranged by size:
2174 1215 1071 742 666 399 85
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 3616 4241 5971 3616
Size: 625 1730 3997
Fragments arranged by size:
3997 1730 625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3707 844
Size: 2863 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4239 1394
Size: 429 2416 3507
Fragments arranged by size:
3507 2416 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2255 4847 5021 65
Size: 1183 1007 2592 174 1396
Fragments arranged by size:
2592 1396 1183 1007 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4996 5183 1700
Size: 110 3186 187 2869
Fragments arranged by size:
3186 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6352
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 4412 5424 4412
Size: 1012 5340
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6352
BstBI TT'CG_AA
Cuts at: 3034 3610 5156 3034
Size: 576 1546 4230
Fragments arranged by size:
4230 1546 576
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstYI r'GATC_y
Cuts at: 1972 2924 2968 3176 3456 3682 4049 4434 4451
Size: 952 44 208 280 226 367 385 17
Cuts at: 4451 5903 5989 6001 6347 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 952 385 367 346 280 226
208 86 44 17 12
BstZ17I GTA'TAC
Cuts at: 2612 2612
Size: 6352
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5800
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5613
Fragments arranged by size:
5613 683 56
BtsI GCAGTG_nn'
Cuts at: 2485 2958 3868 4662 4682 2485
Size: 473 910 794 20 4155
Fragments arranged by size:
4155 910 794 473 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2581 2616 2887 2922 529
Size: 35 2017 35 271 35 3959
Fragments arranged by size:
3959 2017 271 35 35 35
DraI TTT'AAA
Cuts at: 2949 3656 2949
Size: 707 5645
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 3799 4688
Size: 54 1425 50 275 18 198 1771 889
Cuts at: 4688 6317 8
Size: 1629 43
Fragments arranged by size:
1771 1629 1425 889 275 198 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2079 4282 1353
Size: 701 25 2203 3423
Fragments arranged by size:
3423 2203 701 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2106 2147 2176 2248 2397 2401
Size: 393 398 76 41 29 72 149 4
Cuts at: 2401 2501 2730 2814 3234 3318 3334 3486 3490
Size: 100 229 84 420 84 16 152 4
Cuts at: 3490 3570 3574 4415 5012 5799 6067 1239
Size: 80 4 841 597 787 268 1524
Fragments arranged by size:
1524 841 787 597 420 398 393 268
229 152 149 100 84 84 80 76
72 41 29 16 4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2165 2752 2165
Size: 587 5765
EcoO109I rG'GnC_Cy
Cuts at: 3138 3138
Size: 6352
EcoRI G'AATT_C
Cuts at: 3036 3288 3661 4039 3036
Size: 252 373 378 5349
Fragments arranged by size:
5349 378 373 252
EcoRV GAT'ATC
Cuts at: 2042 2042
Size: 6352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2156 2989 2156
Size: 833 5519
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 3384 5499 1678
Size: 1706 2115 2531
Fragments arranged by size:
2531 2115 1706
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 2217 2249 3629
Size: 32 135 32 459 32 154 32 1380
Cuts at: 3629 3661 4993 5025 5067 5099 1373
Size: 32 1332 32 42 32 2626
Fragments arranged by size:
2626 1380 1332 459 154 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2765 2858 3442 3696 3942 669
Size: 988 281 827 93 584 254 246 3079
Fragments arranged by size:
3079 988 827 584 281 254 246 93
HindIII A'AGCT_T
Cuts at: 748 2118 2466 748
Size: 1370 348 4634
Fragments arranged by size:
4634 1370 348
HpaI GTT'AAC
Cuts at: 3442 3942 3442
Size: 500 5852
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5466 5650 1305
Size: 4161 184 2007
Fragments arranged by size:
4161 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 10
Size: 54 1425 343 198 4332
Fragments arranged by size:
4332 1425 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2323 2877 3397 3401 3633 4310 4669
Size: 648 1157 554 520 4 232 677 359
Cuts at: 4669 4828 518
Size: 159 2042
Fragments arranged by size:
2042 1157 677 648 554 520 359 232
159 4
MspA1I CmG'CkG
Cuts at: 1606 3768 4445 5593 5838 1606
Size: 2162 677 1148 245 2120
Fragments arranged by size:
2162 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5800
NdeI CA'TA_TG
Cuts at: 387 2862 387
Size: 2475 3877
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 2294 3116 3370 3454 3632 3710 5255
Size: 97 1065 822 254 84 178 78 1545
Cuts at: 5255 1132
Size: 2229
Fragments arranged by size:
2229 1545 1065 822 254 178 97 84
78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6352
PciI A'CATG_T
Cuts at: 2290 5251 2290
Size: 2961 3391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6352
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PmlI CAC'GTG
Cuts at: 3399 3435 3399
Size: 36 6316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 2808 3922 2808
Size: 1114 5238
PstI C_TGCA'G
Cuts at: 830 3704 830
Size: 2874 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 6352
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SexAI A'CCwGG_T
Cuts at: 2753 2753
Size: 6352
SfcI C'TryA_G
Cuts at: 826 1045 3700 4835 5516 5707 826
Size: 219 2655 1135 681 191 1471
Fragments arranged by size:
2655 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 2851 4456 5357 5619 820
Size: 197 926 339 569 1605 901 262 1553
Fragments arranged by size:
1605 1553 926 901 569 339 262 197
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3116 3632 3710 1132
Size: 1984 516 78 3774
Fragments arranged by size:
3774 1984 516 78
SspI AAT'ATT
Cuts at: 5 52 2689 4276 5
Size: 47 2637 1587 2081
Fragments arranged by size:
2637 2081 1587 47
StuI AGG'CCT
Cuts at: 1517 3167 1517
Size: 1650 4702
StyI C'CwwG_G
Cuts at: 513 1065 3791 513
Size: 552 2726 3074
Fragments arranged by size:
3074 2726 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2867 4379 4396 4549 4734 5179 325
Size: 1255 1287 1512 17 153 185 445 1498
Fragments arranged by size:
1512 1498 1287 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4598 6310 96
Size: 274 80 33 51 1443 2621 1712 138
Fragments arranged by size:
2621 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2554 2586 6312 6344 2554
Size: 32 3726 32 2562
Fragments arranged by size:
3726 2562 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 2495 2747 3083 3503 3587 4481 2495
Size: 252 336 420 84 894 4366
Fragments arranged by size:
4366 894 420 336 252 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI