Restriction map for
pFN21AB9771
(Circular) (Six-base) MAPSORT of: pFN21AB9771.seq Check: 9197 from: 1 to: 6319
pFN21AB9771 6319 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 2466 4446 4819 1563
Size: 903 1980 373 3063
Fragments arranged by size:
3063 1980 903 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2554 4382 5766 6034 1632
Size: 922 1828 1384 268 1917
Fragments arranged by size:
1917 1828 1384 922 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 2428 2699 5218 6195 1203
Size: 1225 271 2519 977 1327
Fragments arranged by size:
2519 1327 1225 977 271
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 3140 1166
Size: 1974 4345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 2813 3104 4062 5634 2813
Size: 291 958 1572 3498
Fragments arranged by size:
3498 1572 958 291
ApaLI G'TGCA_C
Cuts at: 2193 4376 5532 2193
Size: 2183 1156 2980
Fragments arranged by size:
2980 2183 1156
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 3644 1870
Size: 73 69 1632 4545
Fragments arranged by size:
4545 1632 73 69
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3640 5095 618
Size: 325 217 627 1853 1455 1842
Fragments arranged by size:
1853 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3638 1391
Size: 2247 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3237 928
Size: 971 1338 4010
Fragments arranged by size:
4010 1338 971
BbvCI CC'TCA_GC
Cuts at: 3325 3325
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2903 2937 4510 4544 1903
Size: 34 966 34 1573 34 3678
Fragments arranged by size:
3678 1573 966 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2197 2971 4380 5536 1790
Size: 324 83 774 1409 1156 2573
Fragments arranged by size:
2573 1409 1156 774 324 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3425 4050 5008
Size: 416 53 83 399 24 2008 625 958
Cuts at: 5008 442
Size: 1753
Fragments arranged by size:
2008 1753 958 625 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2912 2909
Size: 3 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2245 3010 4979 1239
Size: 803 203 765 1969 2579
Fragments arranged by size:
2579 1969 803 765 203
Bpu10I CC'TnA_GC
Cuts at: 3090 3325 3090
Size: 235 6084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4444 5309 5607 4444
Size: 865 298 5156
Fragments arranged by size:
5156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4982 882
Size: 617 3483 2219
Fragments arranged by size:
3483 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4126 4508 5143 275
Size: 53 83 186 936 2593 382 635 1451
Fragments arranged by size:
2593 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3262 4752 5150 5424 5571 5982 3262
Size: 1490 398 274 147 411 3599
Fragments arranged by size:
3599 1490 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 1178
Size: 30 165 30 1035 30 5029
Fragments arranged by size:
5029 1035 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2885 2901 3612 1186
Size: 1699 16 711 3893
Fragments arranged by size:
3893 1699 711 16
BseYI C'CCAG_C
Cuts at: 3339 5522 6018 3339
Size: 2183 496 3640
Fragments arranged by size:
3640 2183 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3584 1163
Size: 573 1848 3898
Fragments arranged by size:
3898 1848 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2197 4380 4465 5536 2197
Size: 2183 85 1071 2980
Fragments arranged by size:
2980 2183 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2197 2971 3638 4380 4465 5536
Size: 399 324 83 774 667 742 85 1071
Cuts at: 5536 1391
Size: 2174
Fragments arranged by size:
2174 1071 774 742 667 399 324 85
83
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 3674 844
Size: 1954 876 3489
Fragments arranged by size:
3489 1954 876
BsrBI CCG'CTC
Cuts at: 1394 1823 4206 1394
Size: 429 2383 3507
Fragments arranged by size:
3507 2383 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2175 3213 4814 4988 65
Size: 1183 927 1038 1601 174 1396
Fragments arranged by size:
1601 1396 1183 1038 927 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2440 3459 4963 5150 1700
Size: 110 630 1019 1504 187 2869
Fragments arranged by size:
2869 1504 1019 630 187 110
BsrGI T'GTAC_A
Cuts at: 96 2832 96
Size: 2736 3583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 2246 2355 2375 4379 5391 2246
Size: 109 20 2004 1012 3174
Fragments arranged by size:
3174 2004 1012 109 20
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2737 2813 794
Size: 1943 76 4300
Fragments arranged by size:
4300 1943 76
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 2953 2953
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 2554 3649 4016 4401 4418 5870 5956 5968
Size: 582 1095 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1095 582 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3222 3222
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2762 2875 513
Size: 552 1697 113 3957
Fragments arranged by size:
3957 1697 552 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2114 3175 3835 4629 4649 2114
Size: 1061 660 794 20 3784
Fragments arranged by size:
3784 1061 794 660 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3502 3623 3502
Size: 121 6198
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 3766 4655
Size: 54 1425 50 275 18 310 1626 889
Cuts at: 4655 6284 8
Size: 1629 43
Fragments arranged by size:
1629 1626 1425 889 310 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2701 2840 3171 3308 4249 1353
Size: 1348 139 331 137 941 3423
Fragments arranged by size:
3423 1348 941 331 139 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3584 4906 5280 5426 387
Size: 1064 138 1995 1322 374 146 1280
Fragments arranged by size:
1995 1322 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2245 2554 3010 4382 4979 5766
Size: 393 410 203 309 456 1372 597 787
Cuts at: 5766 6034 1239
Size: 268 1524
Fragments arranged by size:
1524 1372 787 597 456 410 393 309
268 203
EcoNI CCTnn'n_nnAGG
Cuts at: 2101 3540 2101
Size: 1439 4880
EcoO109I rG'GnC_Cy
Cuts at: 2757 3336 2757
Size: 579 5740
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FseI GG_CCGG'CC
Cuts at: 3463 3463
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 5466 1678
Size: 3788 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 3422 3454 3588
Size: 32 135 32 866 32 952 32 134
Cuts at: 3588 3620 4960 4992 5034 5066 1373
Size: 32 1340 32 42 32 2626
Fragments arranged by size:
2626 1340 952 866 135 134 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2409 3663 3909 669
Size: 988 281 471 1254 246 3079
Fragments arranged by size:
3079 1254 988 471 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 2613 3918 2613
Size: 1305 5014
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3140 4277 4636 4795 518
Size: 648 1974 1137 359 159 2042
Fragments arranged by size:
2042 1974 1137 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3735 4412 5560 5805 1606
Size: 2129 677 1148 245 2120
Fragments arranged by size:
2129 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3461 3461
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2762 2875 513
Size: 552 1697 113 3957
Fragments arranged by size:
3957 1697 552 113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 3459 3459
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2432 2703 2828 3677 5222 1132
Size: 97 1203 271 125 849 1545 2229
Fragments arranged by size:
2229 1545 1203 849 271 125 97
PacI TTA_AT'TAA
Cuts at: 2649 2649
Size: 6319
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 2428 2699 5218 2428
Size: 271 2519 3529
Fragments arranged by size:
3529 2519 271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2757 3336 2757
Size: 579 5740
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspXI vC'TCGA_Gb
Cuts at: 2012 2012
Size: 6319
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2578 2610 2578
Size: 32 6287
PstI C_TGCA'G
Cuts at: 830 3115 3671 830
Size: 2285 556 3478
Fragments arranged by size:
3478 2285 556
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SanDI GG'GwC_CC
Cuts at: 3336 3336
Size: 6319
SapI GCTCTTCn'nnn_
Cuts at: 2840 3171 2840
Size: 331 5988
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 2859 2859
Size: 6319
SfcI C'TryA_G
Cuts at: 826 1045 3111 3163 3667 4802 5483 5674 826
Size: 219 2066 52 504 1135 681 191 1471
Fragments arranged by size:
2066 1471 1135 681 504 219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 4423 5324 5586 820
Size: 197 926 69 2411 901 262 1553
Fragments arranged by size:
2411 1553 926 901 262 197 69
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 2828 3677 1132
Size: 1696 849 3774
Fragments arranged by size:
3774 1696 849
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2762 2875 3758 513
Size: 552 1697 113 883 3074
Fragments arranged by size:
3074 1697 883 552 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2344 3407 4346 4363 4516 4701 5146
Size: 1255 764 1063 939 17 153 185 445
Cuts at: 5146 325
Size: 1498
Fragments arranged by size:
1498 1255 1063 939 764 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 4565 6277
Size: 274 80 33 51 1443 855 1733 1712
Cuts at: 6277 96
Size: 138
Fragments arranged by size:
1733 1712 1443 855 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 2012 1943
Size: 69 6250
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PacI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FspAI KasI NarI NruI NsiI PfoI PmlI PspOMI
PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI
XcmI