Restriction map for
pFN21AB9768
(Circular) (Six-base) MAPSORT of: pFN21AB9768.seq Check: 4969 from: 1 to: 7723
pFN21AB9768 7723 bp
With 182 enzymes: SgfI *
July 29, 2015 18:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2790 2790
Size: 7723
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2724 5533 278
Size: 53 83 186 936 1188 2809 2468
Fragments arranged by size:
2809 2468 1188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5066 1937
Size: 3129 4594
Acc65I G'GTAC_C
Cuts at: 2751 5044 2751
Size: 2293 5430
AclI AA'CG_TT
Cuts at: 1563 5850 6223 1563
Size: 4287 373 3063
Fragments arranged by size:
4287 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2145 3745 4063 5786 7170 7438 1632
Size: 513 1600 318 1723 1384 268 1917
Fragments arranged by size:
1917 1723 1600 1384 513 318 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7526
AflIII A'CryG_T
Cuts at: 1203 3591 6622 7599 1203
Size: 2388 3031 977 1327
Fragments arranged by size:
3031 2388 1327 977
AgeI A'CCGG_T
Cuts at: 4923 6554 4923
Size: 1631 6092
AhdI GACnn_n'nnGTC
Cuts at: 6452 6452
Size: 7723
AleI CACnn'nnGTG
Cuts at: 1166 2521 2705 3060 1166
Size: 1355 184 355 5829
Fragments arranged by size:
5829 1355 355 184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2539 1184
Size: 1355 6368
AlwNI CAG_nnn'CTG
Cuts at: 2259 2470 2665 5466 7038 2259
Size: 211 195 2801 1572 2944
Fragments arranged by size:
2944 2801 1572 211 195
ApaI G_GGCC'C
Cuts at: 2320 2354 3437 3453 3574 3603 3724 3926 2320
Size: 34 1083 16 121 29 121 202 6117
Fragments arranged by size:
6117 1083 202 121 121 34 29 16
ApaLI G'TGCA_C
Cuts at: 3026 3653 3785 4103 4459 5780 6936 3026
Size: 627 132 318 356 1321 1156 3813
Fragments arranged by size:
3813 1321 1156 627 356 318 132
ApoI r'AATT_y
Cuts at: 1493 5032 5258 5410 7519 1493
Size: 3539 226 152 2109 1697
Fragments arranged by size:
3539 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 4650 4650
Size: 7723
AseI AT'TA_AT
Cuts at: 160 1032 6277 160
Size: 872 5245 1606
Fragments arranged by size:
5245 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7723
AvaI C'yCGr_G
Cuts at: 1870 1943 2131 3715 3821 3919 3965 4009 4165
Size: 73 188 1584 106 98 46 44 156
Cuts at: 4165 4301 4490 4666 4739 4755 4823 5048 1870
Size: 136 189 176 73 16 68 225 4545
Fragments arranged by size:
4545 1584 225 189 188 176 156 136
106 98 73 73 68 46 44 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4918 4951 4918
Size: 33 7690
BamHI G'GATC_C
Cuts at: 5053 5420 5053
Size: 367 7356
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2119 2542 2751 2810
Size: 325 217 627 303 29 423 209 59
Cuts at: 2810 3015 3265 3544 3610 3686 3853 4262 4410
Size: 205 250 279 66 76 167 409 148
Cuts at: 4410 4432 4846 4900 5044 6499 618
Size: 22 414 54 144 1455 1842
Fragments arranged by size:
1842 1455 627 423 414 409 325 303
279 250 217 209 205 167 148 144
76 66 59 54 29 22
BanII G_rGCy'C
Cuts at: 1391 2138 2320 2354 2411 2586 2631 3369 3437
Size: 747 182 34 57 175 45 738 68
Cuts at: 3437 3453 3536 3574 3603 3711 3724 3926 4010
Size: 16 83 38 29 108 13 202 84
Cuts at: 4010 4429 4779 5042 1391
Size: 419 350 263 4072
Fragments arranged by size:
4072 747 738 419 350 263 202 182
175 108 84 83 68 57 45 38
34 29 16 13
BbeI G_GCGC'C
Cuts at: 3269 3614 3690 3857 4266 4414 4850 4904 3269
Size: 345 76 167 409 148 436 54 6088
Fragments arranged by size:
6088 436 409 345 167 148 76 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2716 928
Size: 971 817 5935
Fragments arranged by size:
5935 971 817
BbvCI CC'TCA_GC
Cuts at: 2450 2450
Size: 7723
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5914 5948 1903
Size: 34 3977 34 3678
Fragments arranged by size:
3977 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5615 6825 5615
Size: 1210 6513
BclI T'GATC_A
Cuts at: 1548 2709 1548
Size: 1161 6562
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2684 2808 3132
Size: 107 122 71 631 605 1012 124 324
Cuts at: 3132 4502 6334 136
Size: 1370 1832 1525
Fragments arranged by size:
1832 1525 1370 1012 631 605 324 124
122 107 71
BglII A'GATC_T
Cuts at: 3147 7718 3147
Size: 4571 3152
BlpI GC'TnA_GC
Cuts at: 3041 5140 3041
Size: 2099 5624
Bme1580I G_kGCm'C
Cuts at: 1790 2320 2354 2639 2813 3030 3437 3453 3549
Size: 530 34 285 174 217 407 16 96
Cuts at: 3549 3574 3603 3657 3724 3789 3926 4107 4463
Size: 25 29 54 67 65 137 181 356
Cuts at: 4463 4526 4895 5784 6940 1790
Size: 63 369 889 1156 2573
Fragments arranged by size:
2573 1156 889 530 407 369 356 285
217 181 174 137 96 67 65 63
54 34 29 25 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2884 5454 6412
Size: 416 53 83 399 24 1467 2570 958
Cuts at: 6412 442
Size: 1753
Fragments arranged by size:
2570 1753 1467 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7723
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 7723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2281 2300 2642 2945 3520 4634 6383 1239
Size: 1042 19 342 303 575 1114 1749 2579
Fragments arranged by size:
2579 1749 1114 1042 575 342 303 19
Bpu10I CC'TnA_GC
Cuts at: 2450 2561 4394 2450
Size: 111 1833 5779
Fragments arranged by size:
5779 1833 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 5848 6713 7011 3005
Size: 2843 865 298 3717
Fragments arranged by size:
3717 2843 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2849 4085 6386 882
Size: 617 1350 1236 2301 2219
Fragments arranged by size:
2301 2219 1350 1236 617
BsaAI yAC'GTr
Cuts at: 493 1204 2634 3592 3738 3784 5017 5514 493
Size: 711 1430 958 146 46 1233 497 2702
Fragments arranged by size:
2702 1430 1233 958 711 497 146 46
BsaBI GATnn'nnATC
Cuts at: 7717 7717
Size: 7723
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2721 3266 3470 3611
Size: 53 83 186 936 1188 545 204 141
Cuts at: 3611 3687 3854 4231 4263 4411 4685 4847 4872
Size: 76 167 377 32 148 274 162 25
Cuts at: 4872 4901 5530 5912 6547 275
Size: 29 629 382 635 1451
Fragments arranged by size:
1451 1188 936 635 629 545 382 377
274 204 186 167 162 148 141 83
76 53 32 29 25
BsaWI w'CCGG_w
Cuts at: 4923 6156 6554 6828 6975 7386 4923
Size: 1233 398 274 147 411 5260
Fragments arranged by size:
5260 1233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 2640 2670 1178
Size: 30 165 30 663 30 544 30 6231
Fragments arranged by size:
6231 663 544 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2561 3422 3953 4987 1186
Size: 901 474 861 531 1034 3922
Fragments arranged by size:
3922 1034 901 861 531 474
BseYI C'CCAG_C
Cuts at: 2141 2410 2670 2909 3172 3556 3947 4043 4883
Size: 269 260 239 263 384 391 96 840
Cuts at: 4883 6926 7422 2141
Size: 2043 496 2442
Fragments arranged by size:
2442 2043 840 496 391 384 269 263
260 239 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 2312 2545 2631 4235 1163
Size: 573 363 213 233 86 1604 4651
Fragments arranged by size:
4651 1604 573 363 233 213 86
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2580 2826 3985 4154 4158 4185
Size: 275 186 579 246 1159 169 4 27
Cuts at: 4185 4260 4377 4692 4696 4815 5173 5934 6962
Size: 75 117 315 4 119 358 761 1028
Cuts at: 6962 1540
Size: 2301
Fragments arranged by size:
2301 1159 1028 761 579 358 315 275
246 186 169 119 117 75 27 4
4
BsiHKAI G_wGCw'C
Cuts at: 2571 3030 3657 3789 4107 4463 5016 5784 5869
Size: 459 627 132 318 356 553 768 85
Cuts at: 5869 6940 2571
Size: 1071 3354
Fragments arranged by size:
3354 1071 768 627 553 459 356 318
132 85
BsmI GAATG_Cn'
Cuts at: 5234 5327 5234
Size: 93 7630
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2320 2354 2411 2571 2586 2631
Size: 399 348 182 34 57 160 15 45
Cuts at: 2631 2639 2813 3030 3369 3437 3453 3536 3549
Size: 8 174 217 339 68 16 83 13
Cuts at: 3549 3574 3603 3657 3711 3724 3789 3926 4010
Size: 25 29 54 54 13 65 137 84
Cuts at: 4010 4107 4429 4463 4526 4779 4895 5016 5042
Size: 97 322 34 63 253 116 121 26
Cuts at: 5042 5784 5869 6940 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 348 339 322 253
217 182 174 160 137 121 116 97
85 84 83 68 65 63 57 54
54 45 34 34 29 26 25 16
15 13 13 8
BspEI T'CCGG_A
Cuts at: 7386 7386
Size: 7723
BspHI T'CATG_A
Cuts at: 5612 7342 5612
Size: 1730 5993
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2778 2790 4617 5078 844
Size: 1934 12 1827 461 3489
Fragments arranged by size:
3489 1934 1827 461 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2265 2956 3013 3045 3332 4417 4712
Size: 429 442 691 57 32 287 1085 295
Cuts at: 4712 5610 1394
Size: 898 3507
Fragments arranged by size:
3507 1085 898 691 442 429 295 287
57 32
BsrDI GCAATG_nn'
Cuts at: 65 1248 6218 6392 65
Size: 1183 4970 174 1396
Fragments arranged by size:
4970 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2284 2539 2813 3660 3689 4153 4180
Size: 110 474 255 274 847 29 464 27
Cuts at: 4180 4259 4435 4661 4687 4843 4897 4923 6367
Size: 79 176 226 26 156 54 26 1444
Cuts at: 6367 6554 1700
Size: 187 2869
Fragments arranged by size:
2869 1444 847 474 464 274 255 226
187 176 156 110 79 54 29 27
26 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7723
BssHII G'CGCG_C
Cuts at: 1925 3696 3839 3873 3932 4355 4545 4650 1925
Size: 1771 143 34 59 423 190 105 4998
Fragments arranged by size:
4998 1771 423 190 143 105 59 34
BssSI C'ACGA_G
Cuts at: 4088 4454 4519 5783 6795 4088
Size: 366 65 1264 1012 5016
Fragments arranged by size:
5016 1264 1012 366 65
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2904 4056 4467 4639 794
Size: 2110 1152 411 172 3878
Fragments arranged by size:
3878 2110 1152 411 172
BstBI TT'CG_AA
Cuts at: 6527 6527
Size: 7723
BstEII G'GTnAC_C
Cuts at: 1184 2530 3106 1184
Size: 1346 576 5801
Fragments arranged by size:
5801 1346 576
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 2206 2923 3062 3118 2101
Size: 105 717 139 56 6706
Fragments arranged by size:
6706 717 139 105 56
BstYI r'GATC_y
Cuts at: 1972 2496 2928 3147 3627 5053 5420 5805 5822
Size: 524 432 219 480 1426 367 385 17
Cuts at: 5822 7274 7360 7372 7718 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1426 524 480 432 385 367
346 219 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2115 2235 2334 2359 3249 4020 4310
Size: 552 1050 120 99 25 890 771 290
Cuts at: 4310 4371 4406 4696 4747 513
Size: 61 35 290 51 3489
Fragments arranged by size:
3489 1050 890 771 552 290 290 120
99 61 51 35 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3261 4957 487
Size: 683 56 2035 1696 3253
Fragments arranged by size:
3253 2035 1696 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 5239 6033 6053 2712
Size: 2527 794 20 4382
Fragments arranged by size:
4382 2527 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7723
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2722 2757 529
Size: 35 2158 35 5495
Fragments arranged by size:
5495 2158 35 35
DraI TTT'AAA
Cuts at: 5027 5027
Size: 7723
DraIII CAC_nnn'GTG
Cuts at: 2608 4056 2608
Size: 1448 6275
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4871 6730 809
Size: 507 3555 1859 1802
Fragments arranged by size:
3555 1859 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2577 2599 2823
Size: 54 1425 50 275 18 747 22 224
Cuts at: 2823 3303 3495 3564 3633 3883 3957 3982 4155
Size: 480 192 69 69 250 74 25 173
Cuts at: 4155 4182 4257 4285 4374 4443 4659 4689 4812
Size: 27 75 28 89 69 216 30 123
Cuts at: 4812 5170 6059 7688 8
Size: 358 889 1629 43
Fragments arranged by size:
1629 1425 889 747 480 358 275 250
224 216 192 173 123 89 75 74
69 69 69 54 50 43 30 28
27 25 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2577 2823 3982 4155 4182 4257 4374
Size: 275 765 246 1159 173 27 75 117
Cuts at: 4374 4689 4812 5170 1537
Size: 315 123 358 4090
Fragments arranged by size:
4090 1159 765 358 315 275 246 173
123 117 75 27
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 3716 5653 1353
Size: 885 1478 1937 3423
Fragments arranged by size:
3423 1937 1478 885
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2029 2414 4613 6310 6684 6830
Size: 1064 138 440 385 2199 1697 374 146
Cuts at: 6830 387
Size: 1280
Fragments arranged by size:
2199 1697 1280 1064 440 385 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2281 2300 2642 2945 3520 3745
Size: 393 513 136 19 342 303 575 225
Cuts at: 3745 4063 4634 5786 6383 7170 7438 1239
Size: 318 571 1152 597 787 268 1524
Fragments arranged by size:
1524 1152 787 597 575 571 513 393
342 318 303 268 225 136 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2474 3305 2474
Size: 831 6892
EcoO109I rG'GnC_Cy
Cuts at: 2195 2350 2467 3236 3298 3434 3450 3457 3570
Size: 155 117 769 62 136 16 7 113
Cuts at: 3570 3571 3720 3721 3923 4169 4606 4743 2195
Size: 1 149 1 202 246 437 137 5175
Fragments arranged by size:
5175 769 437 246 202 155 149 137
136 117 113 62 16 7 1 1
EcoRI G'AATT_C
Cuts at: 5032 5410 5032
Size: 378 7345
FseI GG_CCGG'CC
Cuts at: 4184 4665 4184
Size: 481 7242
FspI TGC'GCA
Cuts at: 2939 4220 6229 2939
Size: 1281 2009 4433
Fragments arranged by size:
4433 2009 1281
FspAI rTGC'GCAy
Cuts at: 4220 4220
Size: 7723
HaeII r_GCGC'y
Cuts at: 1678 2916 3269 3614 3690 3735 3796 3857 4266
Size: 1238 353 345 76 45 61 61 409
Cuts at: 4266 4414 4442 4850 4904 6870 1678
Size: 148 28 408 54 1966 2531
Fragments arranged by size:
2531 1966 1238 409 408 353 345 148
76 61 61 54 45 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2313 2345 6364
Size: 32 135 32 569 32 140 32 4019
Cuts at: 6364 6396 6438 6470 1373
Size: 32 42 32 2626
Fragments arranged by size:
4019 2626 569 140 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2155 3772 5067 5313 669
Size: 988 281 217 1617 1295 246 3079
Fragments arranged by size:
3079 1617 1295 988 281 246 217
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7723
HpaI GTT'AAC
Cuts at: 2155 5313 2155
Size: 3158 4565
KasI G'GCGC_C
Cuts at: 3265 3610 3686 3853 4262 4410 4846 4900 3265
Size: 345 76 167 409 148 436 54 6088
Fragments arranged by size:
6088 436 409 345 167 148 76 54
KpnI G_GTAC'C
Cuts at: 2755 5048 2755
Size: 2293 5430
MfeI C'AATT_G
Cuts at: 5322 5322
Size: 7723
MluI A'CGCG_T
Cuts at: 7599 7599
Size: 7723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6837 7021 1305
Size: 5532 184 2007
Fragments arranged by size:
5532 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3635 10
Size: 54 1425 343 1803 4098
Fragments arranged by size:
4098 1803 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 2521 2705 3060 3392 5681 6040
Size: 648 1198 157 184 355 332 2289 359
Cuts at: 6040 6199 518
Size: 159 2042
Fragments arranged by size:
2289 2042 1198 648 359 355 332 184
159 157
MspA1I CmG'CkG
Cuts at: 1606 2170 2949 3060 3251 4312 4373 4408 4529
Size: 564 779 111 191 1061 61 35 121
Cuts at: 4529 4698 4906 5139 5816 6964 7209 1606
Size: 169 208 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 1061 779 677 564 245 233
208 191 169 121 111 61 35
NaeI GCC'GGC
Cuts at: 3662 3691 4182 4261 4663 4689 4845 4899 3662
Size: 29 491 79 402 26 156 54 6486
Fragments arranged by size:
6486 491 402 156 79 54 29 26
NarI GG'CG_CC
Cuts at: 3266 3611 3687 3854 4263 4411 4847 4901 3266
Size: 345 76 167 409 148 436 54 6088
Fragments arranged by size:
6088 436 409 345 167 148 76 54
NcoI C'CATG_G
Cuts at: 513 1065 2359 513
Size: 552 1294 5877
Fragments arranged by size:
5877 1294 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7723
NgoMIV G'CCGG_C
Cuts at: 3660 3689 4180 4259 4661 4687 4843 4897 3660
Size: 29 491 79 402 26 156 54 6486
Fragments arranged by size:
6486 491 402 156 79 54 29 26
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7723
NotI GC'GGCC_GC
Cuts at: 5170 5170
Size: 7723
NspI r_CATG'y
Cuts at: 1132 1229 2938 5081 6626 1132
Size: 97 1709 2143 1545 2229
Fragments arranged by size:
2229 2143 1709 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4175 1348
Size: 2827 4896
PciI A'CATG_T
Cuts at: 6622 6622
Size: 7723
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7723
PmeI GTTT'AAAC
Cuts at: 5027 5027
Size: 7723
PmlI CAC'GTG
Cuts at: 2634 5017 2634
Size: 2383 5340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5792 5824 7305 7337 5792
Size: 32 1481 32 6178
Fragments arranged by size:
6178 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4169 4606 4743 4169
Size: 437 137 7149
Fragments arranged by size:
7149 437 137
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7723
PsiI TTA'TAA
Cuts at: 5293 5293
Size: 7723
PspOMI G'GGCC_C
Cuts at: 2316 2350 3433 3449 3570 3599 3720 3922 2316
Size: 34 1083 16 121 29 121 202 6117
Fragments arranged by size:
6117 1083 202 121 121 34 29 16
PspXI vC'TCGA_Gb
Cuts at: 4490 4490
Size: 7723
PstI C_TGCA'G
Cuts at: 830 2775 3669 5075 830
Size: 1945 894 1406 3478
Fragments arranged by size:
3478 1945 1406 894
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7723
PvuII CAG'CTG
Cuts at: 3060 3060
Size: 7723
SacII CC_GC'GG
Cuts at: 3252 4313 4374 4409 4699 3252
Size: 1061 61 35 290 6276
Fragments arranged by size:
6276 1061 290 61 35
SalI G'TCGA_C
Cuts at: 1936 5065 1936
Size: 3129 4594
SanDI GG'GwC_CC
Cuts at: 4169 4743 4169
Size: 574 7149
SapI GCTCTTCn'nnn_
Cuts at: 2238 3716 2238
Size: 1478 6245
SbfI CC_TGCA'GG
Cuts at: 2775 5075 2775
Size: 2300 5423
ScaI AGT'ACT
Cuts at: 4517 5971 7683 4517
Size: 1454 1712 4557
Fragments arranged by size:
4557 1712 1454
SfcI C'TryA_G
Cuts at: 826 1045 2771 3094 3665 5071 6206 6887 7078
Size: 219 1726 323 571 1406 1135 681 191
Cuts at: 7078 826
Size: 1471
Fragments arranged by size:
1726 1471 1406 1135 681 571 323 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2684 2684
Size: 7723
SfoI GGC'GCC
Cuts at: 3267 3612 3688 3855 4264 4412 4848 4902 3267
Size: 345 76 167 409 148 436 54 6088
Fragments arranged by size:
6088 436 409 345 167 148 76 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7723
SgrAI Cr'CCGG_yG
Cuts at: 1700 3689 4435 4843 4897 1700
Size: 1989 746 408 54 4526
Fragments arranged by size:
4526 1989 746 408 54
SmaI CCC'GGG
Cuts at: 1872 3717 3921 4011 4167 4668 4741 4757 5050
Size: 1845 204 90 156 501 73 16 293
Cuts at: 5050 1872
Size: 4545
Fragments arranged by size:
4545 1845 501 293 204 156 90 73
16
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3020 4490 5827 6728 6990 820
Size: 197 926 1077 1470 1337 901 262 1553
Fragments arranged by size:
1553 1470 1337 1077 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5514 493
Size: 5021 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7723
SphI G_CATG'C
Cuts at: 1132 5081 1132
Size: 3949 3774
SspI AAT'ATT
Cuts at: 5 52 5647 5
Size: 47 5595 2081
Fragments arranged by size:
5595 2081 47
StuI AGG'CCT
Cuts at: 1517 2441 4064 4496 1517
Size: 924 1623 432 4744
Fragments arranged by size:
4744 1623 924 432
StyI C'CwwG_G
Cuts at: 513 1065 2199 2359 2997 3138 3726 3849 5162
Size: 552 1134 160 638 141 588 123 1313
Cuts at: 5162 513
Size: 3074
Fragments arranged by size:
3074 1313 1134 638 588 552 160 141
123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 3186 5750 5767 5920 6105 6550
Size: 1255 498 1108 2564 17 153 185 445
Cuts at: 6550 325
Size: 1498
Fragments arranged by size:
2564 1498 1255 1108 498 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4515 5969 7681
Size: 274 80 33 51 1443 2538 1454 1712
Cuts at: 7681 96
Size: 138
Fragments arranged by size:
2538 1712 1454 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2640 2672 7683 7715 2640
Size: 32 5011 32 2648
Fragments arranged by size:
5011 2648 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3472 1316
Size: 2156 5567
XbaI T'CTAG_A
Cuts at: 5059 5059
Size: 7723
XhoI C'TCGA_G
Cuts at: 1943 4490 1943
Size: 2547 5176
XmaI C'CCGG_G
Cuts at: 1870 3715 3919 4009 4165 4666 4739 4755 5048
Size: 1845 204 90 156 501 73 16 293
Cuts at: 5048 1870
Size: 4545
Fragments arranged by size:
4545 1845 501 293 204 156 90 73
16
XmnI GAAnn'nnTTC
Cuts at: 5852 5852
Size: 7723
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2722 5531 276
Size: 53 83 186 936 1188 2809 2468
Fragments arranged by size:
2809 2468 1188 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BfrBI BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoRV FalI NruI NsiI PacI PfoI PsrI
RsrII SacI SexAI SrfI SwaI XcmI