Restriction map for
pFN21AB9767
(Circular) (Six-base) MAPSORT of: pFN21AB9767.seq Check: 9257 from: 1 to: 7546
pFN21AB9767 7546 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5356 278
Size: 53 83 186 936 3820 2468
Fragments arranged by size:
3820 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2020 4889 1937
Size: 83 2869 4594
Fragments arranged by size:
4594 2869 83
Acc65I G'GTAC_C
Cuts at: 4787 4867 4787
Size: 80 7466
AclI AA'CG_TT
Cuts at: 1563 5673 6046 1563
Size: 4110 373 3063
Fragments arranged by size:
4110 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2262 2574 2655 3083 3246 3266 3270
Size: 452 178 312 81 428 163 20 4
Cuts at: 3270 3528 3966 4116 5609 6993 7261 1632
Size: 258 438 150 1493 1384 268 1917
Fragments arranged by size:
1917 1493 1384 452 438 428 312 268
258 178 163 150 81 20 4
AfeI AGC'GCT
Cuts at: 2249 2249
Size: 7546
AflII C'TTAA_G
Cuts at: 820 1017 3973 820
Size: 197 2956 4393
Fragments arranged by size:
4393 2956 197
AflIII A'CryG_T
Cuts at: 1203 4186 6445 7422 1203
Size: 2983 2259 977 1327
Fragments arranged by size:
2983 2259 1327 977
AgeI A'CCGG_T
Cuts at: 6377 6377
Size: 7546
AhdI GACnn_n'nnGTC
Cuts at: 6275 6275
Size: 7546
AleI CACnn'nnGTG
Cuts at: 1166 2832 1166
Size: 1666 5880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7546
AlwNI CAG_nnn'CTG
Cuts at: 3953 5289 6861 3953
Size: 1336 1572 4638
Fragments arranged by size:
4638 1572 1336
ApaLI G'TGCA_C
Cuts at: 5603 6759 5603
Size: 1156 6390
ApoI r'AATT_y
Cuts at: 1493 2532 2693 3011 3844 4275 4855 5081 5233
Size: 1039 161 318 833 431 580 226 152
Cuts at: 5233 7342 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1039 833 580 431 318 226
161 152
AseI AT'TA_AT
Cuts at: 160 1032 3032 6100 160
Size: 872 2000 3068 1606
Fragments arranged by size:
3068 2000 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
AvaI C'yCGr_G
Cuts at: 1870 1943 4871 1870
Size: 73 2928 4545
Fragments arranged by size:
4545 2928 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2087 2120 2087
Size: 33 7513
BamHI G'GATC_C
Cuts at: 4876 5243 4876
Size: 367 7179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4787 4867 6322 618
Size: 325 217 627 3000 80 1455 1842
Fragments arranged by size:
3000 1842 1455 627 325 217 80
BanII G_rGCy'C
Cuts at: 1391 4479 4865 1391
Size: 3088 386 4072
Fragments arranged by size:
4072 3088 386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2298 2804 928
Size: 971 399 506 5670
Fragments arranged by size:
5670 971 506 399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5737 5771 1903
Size: 34 3800 34 3678
Fragments arranged by size:
3800 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5438 6648 5438
Size: 1210 6336
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7546
BfrBI ATG'CAT
Cuts at: 2879 3005 4086 4330 2879
Size: 126 1081 244 6095
Fragments arranged by size:
6095 1081 244 126
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6157 136
Size: 107 122 71 631 605 4485 1525
Fragments arranged by size:
4485 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7541 7541
Size: 7546
BlpI GC'TnA_GC
Cuts at: 4963 4963
Size: 7546
Bme1580I G_kGCm'C
Cuts at: 1790 5607 6763 1790
Size: 3817 1156 2573
Fragments arranged by size:
3817 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5277 6235 442
Size: 416 53 83 399 24 3860 958 1753
Fragments arranged by size:
3860 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2358 2584 3048 3299 3603 4680 6206 1239
Size: 1119 226 464 251 304 1077 1526 2579
Fragments arranged by size:
2579 1526 1119 1077 464 304 251 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4569 4683 5671 6536 6834 4569
Size: 114 988 865 298 5281
Fragments arranged by size:
5281 988 865 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3730 6209 882
Size: 617 2231 2479 2219
Fragments arranged by size:
2479 2231 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5337 493
Size: 711 4133 2702
Fragments arranged by size:
4133 2702 711
BsaBI GATnn'nnATC
Cuts at: 7540 7540
Size: 7546
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4110 5353 5735 6370
Size: 53 83 186 936 2577 1243 382 635
Cuts at: 6370 275
Size: 1451
Fragments arranged by size:
2577 1451 1243 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5979 6377 6651 6798 7209 5979
Size: 398 274 147 411 6316
Fragments arranged by size:
6316 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2577 2607 2644 2674 1178
Size: 30 165 30 1174 30 37 30 6050
Fragments arranged by size:
6050 1174 165 37 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2037 3211 4777 4780 4783 1186
Size: 851 1174 1566 3 3 3949
Fragments arranged by size:
3949 1566 1174 851 3 3
BseYI C'CCAG_C
Cuts at: 2498 6749 7245 2498
Size: 4251 496 2799
Fragments arranged by size:
4251 2799 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6973
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4996 5757 6785 1540
Size: 275 186 2995 761 1028 2301
Fragments arranged by size:
2995 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4479 4658 5607 5692 6763 4479
Size: 179 949 85 1071 5262
Fragments arranged by size:
5262 1071 949 179 85
BsmI GAATG_Cn'
Cuts at: 3099 4088 4414 5057 5150 3099
Size: 989 326 643 93 5495
Fragments arranged by size:
5495 989 643 326 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4479 4658 4865 5607 5692 6763 1391
Size: 399 2689 179 207 742 85 1071 2174
Fragments arranged by size:
2689 2174 1071 742 399 207 179 85
BspEI T'CCGG_A
Cuts at: 7209 7209
Size: 7546
BspHI T'CATG_A
Cuts at: 3039 5435 7165 3039
Size: 2396 1730 3420
Fragments arranged by size:
3420 2396 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4901 844
Size: 4057 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5433 1394
Size: 429 3610 3507
Fragments arranged by size:
3610 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3412 4785 6041 6215 65
Size: 1183 2164 1373 1256 174 1396
Fragments arranged by size:
2164 1396 1373 1256 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6190 6377 1700
Size: 110 4380 187 2869
Fragments arranged by size:
4380 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3301 4384 96
Size: 3205 1083 3258
Fragments arranged by size:
3258 3205 1083
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7546
BssSI C'ACGA_G
Cuts at: 5606 6618 5606
Size: 1012 6534
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7546
BstBI TT'CG_AA
Cuts at: 3111 6350 3111
Size: 3239 4307
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7546
BstYI r'GATC_y
Cuts at: 1972 2956 3129 4600 4876 5243 5628 5645 7097
Size: 984 173 1471 276 367 385 17 1452
Cuts at: 7097 7183 7195 7541 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1471 1452 984 385 367 346 276
173 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4288 4288
Size: 7546
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6994
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6807
Fragments arranged by size:
6807 683 56
BtsI GCAGTG_nn'
Cuts at: 2726 3973 3997 5062 5856 5876 2726
Size: 1247 24 1065 794 20 4396
Fragments arranged by size:
4396 1247 1065 794 24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7546
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7511
DraI TTT'AAA
Cuts at: 2232 4850 2232
Size: 2618 4928
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6553 809
Size: 507 5237 1802
Fragments arranged by size:
5237 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4993 5882 7511
Size: 54 1425 50 275 18 3163 889 1629
Cuts at: 7511 8
Size: 43
Fragments arranged by size:
3163 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4993 1537
Size: 275 3181 4090
Fragments arranged by size:
4090 3181 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 2633 3241 3985 4484 4659 5476 1353
Size: 1053 227 608 744 499 175 817 3423
Fragments arranged by size:
3423 1053 817 744 608 499 227 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6133 6507 6653 387
Size: 1064 138 4544 374 146 1280
Fragments arranged by size:
4544 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4477 4477
Size: 7546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2262 2358 2574 2584 2655 3048
Size: 393 452 178 96 216 10 71 393
Cuts at: 3048 3083 3246 3266 3270 3299 3528 3603 3966
Size: 35 163 20 4 29 229 75 363
Cuts at: 3966 4116 4680 5609 6206 6993 7261 1239
Size: 150 564 929 597 787 268 1524
Fragments arranged by size:
1524 929 787 597 564 452 393 393
363 268 229 216 178 163 150 96
75 71 35 29 20 10 4
EcoNI CCTnn'n_nnAGG
Cuts at: 4287 4287
Size: 7546
EcoRI G'AATT_C
Cuts at: 4855 5233 4855
Size: 378 7168
EcoRV GAT'ATC
Cuts at: 3181 3181
Size: 7546
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2870 3645 3800 2266
Size: 604 775 155 6012
Fragments arranged by size:
6012 775 604 155
FspI TGC'GCA
Cuts at: 6052 6052
Size: 7546
HaeII r_GCGC'y
Cuts at: 1678 2251 6693 1678
Size: 573 4442 2531
Fragments arranged by size:
4442 2531 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2577 2609 3962 3986 3994
Size: 32 135 32 1005 32 1353 24 8
Cuts at: 3994 4018 4130 4162 6187 6219 6261 6293 1373
Size: 24 112 32 2025 32 42 32 2626
Fragments arranged by size:
2626 2025 1353 1005 135 112 42 32
32 32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2021 2041 2596 2902 4890 5136
Size: 988 281 83 20 555 306 1988 246
Cuts at: 5136 669
Size: 3079
Fragments arranged by size:
3079 1988 988 555 306 281 246 83
20
HindIII A'AGCT_T
Cuts at: 748 3278 748
Size: 2530 5016
HpaI GTT'AAC
Cuts at: 2041 5136 2041
Size: 3095 4451
KpnI G_GTAC'C
Cuts at: 4791 4871 4791
Size: 80 7466
MfeI C'AATT_G
Cuts at: 5145 5145
Size: 7546
MluI A'CGCG_T
Cuts at: 7422 7422
Size: 7546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2726 6660 6844 1305
Size: 1421 3934 184 2007
Fragments arranged by size:
3934 2007 1421 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5724
Fragments arranged by size:
5724 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2832 2878 4322 4355 4796 5504 5863
Size: 648 1666 46 1444 33 441 708 359
Cuts at: 5863 6022 518
Size: 159 2042
Fragments arranged by size:
2042 1666 1444 708 648 441 359 159
46 33
MspA1I CmG'CkG
Cuts at: 1606 4962 5639 6787 7032 1606
Size: 3356 677 1148 245 2120
Fragments arranged by size:
3356 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6994
NdeI CA'TA_TG
Cuts at: 387 2875 387
Size: 2488 5058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7546
NotI GC'GGCC_GC
Cuts at: 4993 4993
Size: 7546
NsiI A_TGCA'T
Cuts at: 2881 3007 4088 4332 2881
Size: 126 1081 244 6095
Fragments arranged by size:
6095 1081 244 126
NspI r_CATG'y
Cuts at: 1132 1229 3726 4190 4904 6449 1132
Size: 97 2497 464 714 1545 2229
Fragments arranged by size:
2497 2229 1545 714 464 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7546
PciI A'CATG_T
Cuts at: 4186 6445 4186
Size: 2259 5287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4716 1108
Size: 3608 3938
PmeI GTTT'AAAC
Cuts at: 4850 4850
Size: 7546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5615 5647 7128 7160 5615
Size: 32 1481 32 6001
Fragments arranged by size:
6001 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7546
PsiI TTA'TAA
Cuts at: 2213 2892 5116 2213
Size: 679 2224 4643
Fragments arranged by size:
4643 2224 679
PstI C_TGCA'G
Cuts at: 830 4898 830
Size: 4068 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7546
SacI G_AGCT'C
Cuts at: 4479 4479
Size: 7546
SalI G'TCGA_C
Cuts at: 1936 2019 4888 1936
Size: 83 2869 4594
Fragments arranged by size:
4594 2869 83
SapI GCTCTTCn'nnn_
Cuts at: 2406 3985 4484 4659 2406
Size: 1579 499 175 5293
Fragments arranged by size:
5293 1579 499 175
SbfI CC_TGCA'GG
Cuts at: 4898 4898
Size: 7546
ScaI AGT'ACT
Cuts at: 5794 7506 5794
Size: 1712 5834
SfcI C'TryA_G
Cuts at: 826 1045 2830 4620 4894 6029 6710 6901 826
Size: 219 1785 1790 274 1135 681 191 1471
Fragments arranged by size:
1790 1785 1471 1135 681 274 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7546
SmaI CCC'GGG
Cuts at: 1872 4873 1872
Size: 3001 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3973 4584 4698 5650 6551 6813
Size: 197 926 2030 611 114 952 901 262
Cuts at: 6813 820
Size: 1553
Fragments arranged by size:
2030 1553 952 926 901 611 262 197
114
SnaBI TAC'GTA
Cuts at: 493 5337 493
Size: 4844 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7546
SphI G_CATG'C
Cuts at: 1132 4904 1132
Size: 3772 3774
SspI AAT'ATT
Cuts at: 5 52 2264 5470 5
Size: 47 2212 3206 2081
Fragments arranged by size:
3206 2212 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7546
StyI C'CwwG_G
Cuts at: 513 1065 4985 513
Size: 552 3920 3074
Fragments arranged by size:
3920 3074 552
SwaI ATTT'AAAT
Cuts at: 2232 2232
Size: 7546
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2414 2512 5573 5590 5743 5928 6373
Size: 1255 834 98 3061 17 153 185 445
Cuts at: 6373 325
Size: 1498
Fragments arranged by size:
3061 1498 1255 834 445 185 153 98
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3054 3301 4384
Size: 274 80 33 51 1443 1077 247 1083
Cuts at: 4384 5792 7504 96
Size: 1408 1712 138
Fragments arranged by size:
1712 1443 1408 1083 1077 274 247 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4116 4148 7506 7538 4116
Size: 32 3358 32 4124
Fragments arranged by size:
4124 3358 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2939 1316
Size: 1623 5923
XbaI T'CTAG_A
Cuts at: 2368 3848 4882 2368
Size: 1480 1034 5032
Fragments arranged by size:
5032 1480 1034
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7546
XmaI C'CCGG_G
Cuts at: 1870 4871 1870
Size: 3001 4545
XmnI GAAnn'nnTTC
Cuts at: 3783 5675 3783
Size: 1892 5654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5354 276
Size: 53 83 186 936 3820 2468
Fragments arranged by size:
3820 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI XcmI