Restriction map for
pFN21AB9765
(Circular) (Six-base) MAPSORT of: pFN21AB9765.seq Check: 2241 from: 1 to: 7414
pFN21AB9765 7414 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5224 278
Size: 53 83 186 936 3688 2468
Fragments arranged by size:
3688 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4383 4665 4757 1937
Size: 2446 282 92 4594
Fragments arranged by size:
4594 2446 282 92
Acc65I G'GTAC_C
Cuts at: 4735 4735
Size: 7414
AclI AA'CG_TT
Cuts at: 1563 2053 5541 5914 1563
Size: 490 3488 373 3063
Fragments arranged by size:
3488 3063 490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3051 5477 6861 7129 1632
Size: 1419 2426 1384 268 1917
Fragments arranged by size:
2426 1917 1419 1384 268
AfeI AGC'GCT
Cuts at: 3590 3590
Size: 7414
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7217
AflIII A'CryG_T
Cuts at: 1203 3429 4554 4617 6313 7290 1203
Size: 2226 1125 63 1696 977 1327
Fragments arranged by size:
2226 1696 1327 1125 977 63
AgeI A'CCGG_T
Cuts at: 6245 6245
Size: 7414
AhdI GACnn_n'nnGTC
Cuts at: 3307 4153 6143 3307
Size: 846 1990 4578
Fragments arranged by size:
4578 1990 846
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4334 4463 1184
Size: 3150 129 4135
Fragments arranged by size:
4135 3150 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 7382
AlwNI CAG_nnn'CTG
Cuts at: 2386 3906 4696 5157 6729 2386
Size: 1520 790 461 1572 3071
Fragments arranged by size:
3071 1572 1520 790 461
ApaLI G'TGCA_C
Cuts at: 2307 2331 2741 4331 4541 5471 6627 2307
Size: 24 410 1590 210 930 1156 3094
Fragments arranged by size:
3094 1590 1156 930 410 210 24
ApoI r'AATT_y
Cuts at: 1493 2423 4723 4949 5101 7210 1493
Size: 930 2300 226 152 2109 1697
Fragments arranged by size:
2300 2109 1697 930 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3162 5968 160
Size: 872 2130 2806 1606
Fragments arranged by size:
2806 2130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7414
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2639 2919 4193 4739 1870
Size: 73 139 557 280 1274 546 4545
Fragments arranged by size:
4545 1274 557 546 280 139 73
AvrII C'CTAG_G
Cuts at: 2458 2458
Size: 7414
BamHI G'GATC_C
Cuts at: 2035 4744 5111 2035
Size: 2709 367 4338
Fragments arranged by size:
4338 2709 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 3759 4073 4109 4394
Size: 325 217 627 221 1751 314 36 285
Cuts at: 4394 4735 6190 618
Size: 341 1455 1842
Fragments arranged by size:
1842 1751 1455 627 341 325 314 285
221 217 36
BanII G_rGCy'C
Cuts at: 1391 3408 3782 3813 4733 1391
Size: 2017 374 31 920 4072
Fragments arranged by size:
4072 2017 920 374 31
BbeI G_GCGC'C
Cuts at: 2012 3763 4077 4113 2012
Size: 1751 314 36 5313
Fragments arranged by size:
5313 1751 314 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3039 3402 928
Size: 971 1140 363 4940
Fragments arranged by size:
4940 1140 971 363
BbvCI CC'TCA_GC
Cuts at: 2592 2592
Size: 7414
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5605 5639 1903
Size: 34 3668 34 3678
Fragments arranged by size:
3678 3668 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3129 5306 6516 3129
Size: 2177 1210 4027
Fragments arranged by size:
4027 2177 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7414
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3786 3817 4124
Size: 107 122 71 631 605 2114 31 307
Cuts at: 4124 6025 136
Size: 1901 1525
Fragments arranged by size:
2114 1901 1525 631 605 307 122 107
71 31
BglII A'GATC_T
Cuts at: 3867 3930 7409 3867
Size: 63 3479 3872
Fragments arranged by size:
3872 3479 63
BlpI GC'TnA_GC
Cuts at: 2535 2608 4831 2535
Size: 73 2223 5118
Fragments arranged by size:
5118 2223 73
Bme1580I G_kGCm'C
Cuts at: 1790 2311 2335 2588 2745 4335 4397 4545 5475
Size: 521 24 253 157 1590 62 148 930
Cuts at: 5475 6631 1790
Size: 1156 2573
Fragments arranged by size:
2573 1590 1156 930 521 253 157 148
62 24
BmgBI CAC'GTC
Cuts at: 1318 3037 3642 1318
Size: 1719 605 5090
Fragments arranged by size:
5090 1719 605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2322 2658 4284
Size: 416 53 83 399 24 905 336 1626
Cuts at: 4284 5145 6103 442
Size: 861 958 1753
Fragments arranged by size:
1753 1626 958 905 861 416 399 336
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4593 1056
Size: 3537 3877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3331 3331
Size: 7414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3457 3679 3919 4304 4718 6074 1239
Size: 2218 222 240 385 414 1356 2579
Fragments arranged by size:
2579 2218 1356 414 385 240 222
Bpu10I CC'TnA_GC
Cuts at: 2592 2592
Size: 7414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 3836 5539 6404 6702 2711
Size: 1125 1703 865 298 3423
Fragments arranged by size:
3423 1703 1125 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2576 2627 2849 6077 882
Size: 617 1077 51 222 3228 2219
Fragments arranged by size:
3228 2219 1077 617 222 51
BsaAI yAC'GTr
Cuts at: 493 1204 2524 3595 4039 4540 5205 493
Size: 711 1320 1071 444 501 665 2702
Fragments arranged by size:
2702 1320 1071 711 665 501 444
BsaBI GATnn'nnATC
Cuts at: 3575 7408 3575
Size: 3833 3581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3760 4074 4110
Size: 53 83 186 936 476 1751 314 36
Cuts at: 4110 4172 4514 5221 5603 6238 275
Size: 62 342 707 382 635 1451
Fragments arranged by size:
1751 1451 936 707 635 476 382 342
314 186 83 62 53 36
BsaWI w'CCGG_w
Cuts at: 3411 5847 6245 6519 6666 7077 3411
Size: 2436 398 274 147 411 3748
Fragments arranged by size:
3748 2436 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2508 2538 3338 3368 3590
Size: 30 165 30 1105 30 800 30 222
Cuts at: 3590 3620 1178
Size: 30 4972
Fragments arranged by size:
4972 1105 800 222 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2611 1186
Size: 1425 5989
BseYI C'CCAG_C
Cuts at: 2021 2542 2828 3971 4247 6617 7113 2021
Size: 521 286 1143 276 2370 496 2322
Fragments arranged by size:
2370 2322 1143 521 496 286 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4019 1163
Size: 573 2283 4558
Fragments arranged by size:
4558 2283 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4089 4371 4632 4864 5625
Size: 275 186 1411 677 282 261 232 761
Cuts at: 5625 6653 1540
Size: 1028 2301
Fragments arranged by size:
2301 1411 1028 761 677 282 275 261
232 186
BsiHKAI G_wGCw'C
Cuts at: 2311 2335 2745 3516 3562 3852 3984 4323 4335
Size: 24 410 771 46 290 132 339 12
Cuts at: 4335 4545 5475 5560 6631 2311
Size: 210 930 85 1071 3094
Fragments arranged by size:
3094 1071 930 771 410 339 290 210
132 85 46 24 12
BsmI GAATG_Cn'
Cuts at: 3178 4925 5018 3178
Size: 1747 93 5574
Fragments arranged by size:
5574 1747 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2311 2335 2588 2745 3408 3516 3562
Size: 399 521 24 253 157 663 108 46
Cuts at: 3562 3782 3813 3852 3984 4323 4335 4397 4545
Size: 220 31 39 132 339 12 62 148
Cuts at: 4545 4733 5475 5560 6631 1391
Size: 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 663 521 399 339 253
220 188 157 148 132 108 85 62
46 39 31 24 12
BspEI T'CCGG_A
Cuts at: 7077 7077
Size: 7414
BspHI T'CATG_A
Cuts at: 5303 7033 5303
Size: 1730 5684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3621 4452 4769 844
Size: 2777 831 317 3489
Fragments arranged by size:
3489 2777 831 317
BsrBI CCG'CTC
Cuts at: 1394 1823 4165 5301 1394
Size: 429 2342 1136 3507
Fragments arranged by size:
3507 2342 1136 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5909 6083 65
Size: 1183 4661 174 1396
Fragments arranged by size:
4661 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2784 3756 4545 4610 6058 6245 1700
Size: 110 974 972 789 65 1448 187 2869
Fragments arranged by size:
2869 1448 974 972 789 187 110 65
BsrGI T'GTAC_A
Cuts at: 96 2430 4269 96
Size: 2334 1839 3241
Fragments arranged by size:
3241 2334 1839
BssHII G'CGCG_C
Cuts at: 1925 3684 1925
Size: 1759 5655
BssSI C'ACGA_G
Cuts at: 2846 5474 6486 2846
Size: 2628 1012 3774
Fragments arranged by size:
3774 2628 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2866 4300 794
Size: 2072 1434 3908
Fragments arranged by size:
3908 2072 1434
BstBI TT'CG_AA
Cuts at: 6218 6218
Size: 7414
BstEII G'GTnAC_C
Cuts at: 1184 4339 4420 1184
Size: 3155 81 4178
Fragments arranged by size:
4178 3155 81
BstYI r'GATC_y
Cuts at: 1972 2035 2977 3867 3930 4744 5111 5496 5513
Size: 63 942 890 63 814 367 385 17
Cuts at: 5513 6965 7051 7063 7409 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 942 890 814 385 367 346
86 63 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 3328 3328
Size: 7414
BtgI C'CryG_G
Cuts at: 513 1065 2004 2252 2812 3240 3815 4122 4178
Size: 552 939 248 560 428 575 307 56
Cuts at: 4178 4389 4518 4626 513
Size: 211 129 108 3301
Fragments arranged by size:
3301 939 575 560 552 428 307 248
211 129 108 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3232 3584 487
Size: 683 56 2006 352 4317
Fragments arranged by size:
4317 2006 683 352 56
BtsI GCAGTG_nn'
Cuts at: 2497 2586 4584 4930 5724 5744 2497
Size: 89 1998 346 794 20 4167
Fragments arranged by size:
4167 1998 794 346 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7379
DraI TTT'AAA
Cuts at: 4718 4718
Size: 7414
DraIII CAC_nnn'GTG
Cuts at: 4339 4540 4339
Size: 201 7213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6421 809
Size: 507 5105 1802
Fragments arranged by size:
5105 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 3463 4041
Size: 54 1425 50 275 18 306 1327 578
Cuts at: 4041 4086 4116 4353 4368 4428 4629 4861 5750
Size: 45 30 237 15 60 201 232 889
Cuts at: 5750 7379 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1327 889 578 306 275 237
232 201 60 54 50 45 43 30
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4086 4368 4629 4861 1537
Size: 275 2274 282 261 232 4090
Fragments arranged by size:
4090 2274 282 275 261 232
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2999 4646 5344 1353
Size: 804 842 1647 698 3423
Fragments arranged by size:
3423 1647 842 804 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3739 6001 6375 6521 387
Size: 1064 138 2150 2262 374 146 1280
Fragments arranged by size:
2262 2150 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3051 3457 3679 3919 4304 4718 5477
Size: 393 1419 406 222 240 385 414 759
Cuts at: 5477 6074 6861 7129 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1419 787 759 597 414 406 393
385 268 240 222
EcoNI CCTnn'n_nnAGG
Cuts at: 2579 2579
Size: 7414
EcoO109I rG'GnC_Cy
Cuts at: 2245 2273 4279 4347 2245
Size: 28 2006 68 5312
Fragments arranged by size:
5312 2006 68 28
EcoRI G'AATT_C
Cuts at: 4723 5101 4723
Size: 378 7036
EcoRV GAT'ATC
Cuts at: 2995 2995
Size: 7414
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 7414
FspI TGC'GCA
Cuts at: 2859 5920 2859
Size: 3061 4353
HaeII r_GCGC'y
Cuts at: 1678 2012 3592 3654 3750 3763 4077 4113 4292
Size: 334 1580 62 96 13 314 36 179
Cuts at: 4292 6561 1678
Size: 2269 2531
Fragments arranged by size:
2531 2269 1580 334 314 179 96 62
36 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 3293 3325 3590
Size: 32 135 32 599 32 1090 32 265
Cuts at: 3590 3622 4139 4171 6055 6087 6129 6161 1373
Size: 32 517 32 1884 32 42 32 2626
Fragments arranged by size:
2626 1884 1090 599 517 265 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 4468 4483 4758 5004 669
Size: 988 281 717 1813 15 275 246 3079
Fragments arranged by size:
3079 1813 988 717 281 275 246 15
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7414
HpaI GTT'AAC
Cuts at: 4468 4483 5004 4468
Size: 15 521 6878
Fragments arranged by size:
6878 521 15
KasI G'GCGC_C
Cuts at: 2008 3759 4073 4109 2008
Size: 1751 314 36 5313
Fragments arranged by size:
5313 1751 314 36
KpnI G_GTAC'C
Cuts at: 4739 4739
Size: 7414
MfeI C'AATT_G
Cuts at: 5013 5013
Size: 7414
MluI A'CGCG_T
Cuts at: 3429 7290 3429
Size: 3861 3553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 3324 3387 3588 3669 4170 4281 6528
Size: 1305 714 63 201 81 501 111 2247
Cuts at: 6528 6712 1305
Size: 184 2007
Fragments arranged by size:
2247 2007 1305 714 501 201 184 111
81 63
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 4043 4430 10
Size: 54 1425 343 306 1905 387 2994
Fragments arranged by size:
2994 1905 1425 387 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2711 3197 3239 4523 5372 5731 5890
Size: 648 1545 486 42 1284 849 359 159
Cuts at: 5890 518
Size: 2042
Fragments arranged by size:
2042 1545 1284 849 648 486 359 159
42
MspA1I CmG'CkG
Cuts at: 1606 2542 3817 4124 4153 4180 4327 4830 5507
Size: 936 1275 307 29 27 147 503 677
Cuts at: 5507 6655 6900 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1275 1148 936 677 503 307 245
147 29 27
NaeI GCC'GGC
Cuts at: 2786 3758 4612 2786
Size: 972 854 5588
Fragments arranged by size:
5588 972 854
NarI GG'CG_CC
Cuts at: 2009 3760 4074 4110 2009
Size: 1751 314 36 5313
Fragments arranged by size:
5313 1751 314 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 3240 4518 513
Size: 552 939 1236 1278 3409
Fragments arranged by size:
3409 1278 1236 939 552
NdeI CA'TA_TG
Cuts at: 387 2907 387
Size: 2520 4894
NgoMIV G'CCGG_C
Cuts at: 2784 3756 4610 2784
Size: 972 854 5588
Fragments arranged by size:
5588 972 854
NheI G'CTAG_C
Cuts at: 1052 4589 1052
Size: 3537 3877
NotI GC'GGCC_GC
Cuts at: 4086 4861 4086
Size: 775 6639
NspI r_CATG'y
Cuts at: 1132 1229 2864 4558 4772 6317 1132
Size: 97 1635 1694 214 1545 2229
Fragments arranged by size:
2229 1694 1635 1545 214 97
PasI CC'CwG_GG
Cuts at: 1348 2561 1348
Size: 1213 6201
PciI A'CATG_T
Cuts at: 4554 6313 4554
Size: 1759 5655
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2839 1108
Size: 1731 5683
PfoI T'CCnGG_A
Cuts at: 3935 3935
Size: 7414
PmeI GTTT'AAAC
Cuts at: 4718 4718
Size: 7414
PmlI CAC'GTG
Cuts at: 2524 4039 4540 2524
Size: 1515 501 5398
Fragments arranged by size:
5398 1515 501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5483 5515 6996 7028 5483
Size: 32 1481 32 5869
Fragments arranged by size:
5869 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 4279 4347 2245
Size: 2034 68 5312
Fragments arranged by size:
5312 2034 68
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7414
PsiI TTA'TAA
Cuts at: 4984 4984
Size: 7414
PspXI vC'TCGA_Gb
Cuts at: 2919 2919
Size: 7414
PstI C_TGCA'G
Cuts at: 830 2161 3624 4296 4766 830
Size: 1331 1463 672 470 3478
Fragments arranged by size:
3478 1463 1331 672 470
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7414
PvuII CAG'CTG
Cuts at: 2542 2542
Size: 7414
SacII CC_GC'GG
Cuts at: 3818 4125 4181 3818
Size: 307 56 7051
Fragments arranged by size:
7051 307 56
SalI G'TCGA_C
Cuts at: 1936 4756 1936
Size: 2820 4594
SanDI GG'GwC_CC
Cuts at: 2245 4347 2245
Size: 2102 5312
SbfI CC_TGCA'GG
Cuts at: 4766 4766
Size: 7414
ScaI AGT'ACT
Cuts at: 3098 5662 7374 3098
Size: 2564 1712 3138
Fragments arranged by size:
3138 2564 1712
SfcI C'TryA_G
Cuts at: 826 1045 2157 2988 3620 3952 4292 4666 4762
Size: 219 1112 831 632 332 340 374 96
Cuts at: 4762 5897 6578 6769 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1112 831 681 632 374 340
332 219 191 96
SfiI GGCCn_nnn'nGGCC
Cuts at: 4124 4124
Size: 7414
SfoI GGC'GCC
Cuts at: 2010 3761 4075 4111 2010
Size: 1751 314 36 5313
Fragments arranged by size:
5313 1751 314 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7414
SgrAI Cr'CCGG_yG
Cuts at: 1700 3756 1700
Size: 2056 5358
SmaI CCC'GGG
Cuts at: 1872 4195 4741 1872
Size: 2323 546 4545
Fragments arranged by size:
4545 2323 546
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2690 2919 3851 5518 6419 6681
Size: 197 926 747 229 932 1667 901 262
Cuts at: 6681 820
Size: 1553
Fragments arranged by size:
1667 1553 932 926 901 747 262 229
197
SnaBI TAC'GTA
Cuts at: 493 5205 493
Size: 4712 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7414
SphI G_CATG'C
Cuts at: 1132 2864 4772 1132
Size: 1732 1908 3774
Fragments arranged by size:
3774 1908 1732
SspI AAT'ATT
Cuts at: 5 52 5338 5
Size: 47 5286 2081
Fragments arranged by size:
5286 2081 47
StuI AGG'CCT
Cuts at: 1517 2463 3692 1517
Size: 946 1229 5239
Fragments arranged by size:
5239 1229 946
StyI C'CwwG_G
Cuts at: 513 1065 2004 2458 3240 3534 3654 4518 4853
Size: 552 939 454 782 294 120 864 335
Cuts at: 4853 513
Size: 3074
Fragments arranged by size:
3074 939 864 782 552 454 335 294
120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3187 3253 4045 5441 5458 5611 5796
Size: 1255 1607 66 792 1396 17 153 185
Cuts at: 5796 6241 325
Size: 445 1498
Fragments arranged by size:
1607 1498 1396 1255 792 445 185 153
66 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 3096 3225
Size: 274 80 33 51 1443 453 666 129
Cuts at: 3225 4269 5660 7372 96
Size: 1044 1391 1712 138
Fragments arranged by size:
1712 1443 1391 1044 666 453 274 138
129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 2508 2540 2751 2783 3974 4006 7374
Size: 32 239 32 211 32 1191 32 3368
Cuts at: 7374 7406 2237
Size: 32 2245
Fragments arranged by size:
3368 2245 1191 239 211 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2504 4055 1316
Size: 1188 1551 4675
Fragments arranged by size:
4675 1551 1188
XbaI T'CTAG_A
Cuts at: 4750 4750
Size: 7414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2836 4126 4516 2836
Size: 1290 390 5734
Fragments arranged by size:
5734 1290 390
XhoI C'TCGA_G
Cuts at: 1943 2919 1943
Size: 976 6438
XmaI C'CCGG_G
Cuts at: 1870 4193 4739 1870
Size: 2323 546 4545
Fragments arranged by size:
4545 2323 546
XmnI GAAnn'nnTTC
Cuts at: 5543 5543
Size: 7414
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5222 276
Size: 53 83 186 936 3688 2468
Fragments arranged by size:
3688 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoICRI FseI FspAI NruI NsiI PacI PspOMI
PsrI RsrII SacI SapI SexAI SrfI SwaI