Restriction map for
pFN21AB9760
(Circular) (Six-base) MAPSORT of: pFN21AB9760.seq Check: 3411 from: 1 to: 6922
pFN21AB9760 6922 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2263 2263
Size: 6922
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2619 4265 1937
Size: 682 1646 4594
Fragments arranged by size:
4594 1646 682
Acc65I G'GTAC_C
Cuts at: 3364 4243 3364
Size: 879 6043
AclI AA'CG_TT
Cuts at: 1563 5049 5422 1563
Size: 3486 373 3063
Fragments arranged by size:
3486 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3785 4985 6369 6637 1632
Size: 2153 1200 1384 268 1917
Fragments arranged by size:
2153 1917 1384 1200 268
AfeI AGC'GCT
Cuts at: 2636 2649 2636
Size: 13 6909
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6725
AflIII A'CryG_T
Cuts at: 1203 5821 6798 1203
Size: 4618 977 1327
Fragments arranged by size:
4618 1327 977
AgeI A'CCGG_T
Cuts at: 5753 5753
Size: 6922
AhdI GACnn_n'nnGTC
Cuts at: 5651 5651
Size: 6922
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3920 1184
Size: 2736 4186
AlwNI CAG_nnn'CTG
Cuts at: 2177 2745 3341 3542 3733 4665 6237 2177
Size: 568 596 201 191 932 1572 2862
Fragments arranged by size:
2862 1572 932 596 568 201 191
ApaI G_GGCC'C
Cuts at: 2195 2398 2530 3029 3077 3229 3276 3483 2195
Size: 203 132 499 48 152 47 207 5634
Fragments arranged by size:
5634 499 207 203 152 132 48 47
ApaLI G'TGCA_C
Cuts at: 3551 4979 6135 3551
Size: 1428 1156 4338
Fragments arranged by size:
4338 1428 1156
ApoI r'AATT_y
Cuts at: 1493 4231 4457 4609 6718 1493
Size: 2738 226 152 2109 1697
Fragments arranged by size:
2738 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2793 2793
Size: 6922
AseI AT'TA_AT
Cuts at: 160 1032 5476 160
Size: 872 4444 1606
Fragments arranged by size:
4444 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 2194 2203 2213 2225 2267 2444 3328
Size: 73 251 9 10 12 42 177 884
Cuts at: 3328 3382 4247 1870
Size: 54 865 4545
Fragments arranged by size:
4545 884 865 251 177 73 54 42
12 10 9
AvrII C'CTAG_G
Cuts at: 3844 3844
Size: 6922
BamHI G'GATC_C
Cuts at: 2198 4252 4619 2198
Size: 2054 367 4501
Fragments arranged by size:
4501 2054 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3101 3289 3364 3848 3949
Size: 325 217 627 1314 188 75 484 101
Cuts at: 3949 4195 4243 5698 618
Size: 246 48 1455 1842
Fragments arranged by size:
1842 1455 1314 627 484 325 246 217
188 101 75 48
BanII G_rGCy'C
Cuts at: 1391 2195 2398 2433 2530 2912 3029 3077 3229
Size: 804 203 35 97 382 117 48 152
Cuts at: 3229 3276 3407 3483 4212 4241 1391
Size: 47 131 76 729 29 4072
Fragments arranged by size:
4072 804 729 382 203 152 131 117
97 76 48 47 35 29
BbeI G_GCGC'C
Cuts at: 3953 3953
Size: 6922
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5951
BbvCI CC'TCA_GC
Cuts at: 2187 4085 4164 2187
Size: 1898 79 4945
Fragments arranged by size:
4945 1898 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2882 2916 3634 3668 5113 5147 1903
Size: 34 945 34 718 34 1445 34 3678
Fragments arranged by size:
3678 1445 945 718 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3399 4814 6024 3399
Size: 1415 1210 4297
Fragments arranged by size:
4297 1415 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6922
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2316 2367 2402
Size: 107 122 71 631 605 644 51 35
Cuts at: 2402 2525 3233 3331 5533 136
Size: 123 708 98 2202 1525
Fragments arranged by size:
2202 1525 708 644 631 605 123 122
107 98 71 51 35
BglII A'GATC_T
Cuts at: 6917 6917
Size: 6922
BlpI GC'TnA_GC
Cuts at: 4339 4339
Size: 6922
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2398 2530 3029 3077 3106 3229 3276
Size: 405 203 132 499 48 29 123 47
Cuts at: 3276 3483 3555 4983 6139 1790
Size: 207 72 1428 1156 2573
Fragments arranged by size:
2573 1428 1156 499 405 207 203 132
123 72 48 47 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6922
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2876 3099 4653
Size: 416 53 83 399 24 1459 223 1554
Cuts at: 4653 5611 442
Size: 958 1753
Fragments arranged by size:
1753 1554 1459 958 416 399 223 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2885 3049 3209 3877 4172 5582 1239
Size: 1046 600 164 160 668 295 1410 2579
Fragments arranged by size:
2579 1410 1046 668 600 295 164 160
Bpu10I CC'TnA_GC
Cuts at: 2187 3401 4085 4164 2187
Size: 1214 684 79 4945
Fragments arranged by size:
4945 1214 684 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2344 2636 5047 5912 6210 2344
Size: 292 2411 865 298 3056
Fragments arranged by size:
3056 2411 865 298 292
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3134 3209 5585 882
Size: 617 1635 75 2376 2219
Fragments arranged by size:
2376 2219 1635 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 2366 4713 493
Size: 711 1162 2347 2702
Fragments arranged by size:
2702 2347 1162 711
BsaBI GATnn'nnATC
Cuts at: 2820 6916 2820
Size: 4096 2826
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 3950 4729 5111
Size: 53 83 186 936 675 1742 779 382
Cuts at: 5111 5746 275
Size: 635 1451
Fragments arranged by size:
1742 1451 936 779 675 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2772 5355 5753 6027 6174 6585 2772
Size: 2583 398 274 147 411 3109
Fragments arranged by size:
3109 2583 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2920 2950 3185 3215 3249
Size: 30 165 30 1517 30 235 30 34
Cuts at: 3249 3279 1178
Size: 30 4821
Fragments arranged by size:
4821 1517 235 165 34 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2102 2105 2624 2950 3005 3206 3275
Size: 913 3 3 519 326 55 201 69
Cuts at: 3275 4220 1186
Size: 945 3888
Fragments arranged by size:
3888 945 913 519 326 201 69 55
3 3
BseYI C'CCAG_C
Cuts at: 2045 3004 3119 3815 4057 4182 6125 6621 2045
Size: 959 115 696 242 125 1943 496 2346
Fragments arranged by size:
2346 1943 959 696 496 242 125 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3692 3843 1163
Size: 573 1956 151 4242
Fragments arranged by size:
4242 1956 573 151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2130 2154 2320 2355 2536
Size: 275 186 78 51 24 166 35 181
Cuts at: 2536 2773 3987 4372 5133 6161 1540
Size: 237 1214 385 761 1028 2301
Fragments arranged by size:
2301 1214 1028 761 385 275 237 186
181 166 78 51 35 24
BsiHKAI G_wGCw'C
Cuts at: 2433 3015 3555 4983 5068 6139 2433
Size: 582 540 1428 85 1071 3216
Fragments arranged by size:
3216 1428 1071 582 540 85
BsmI GAATG_Cn'
Cuts at: 4433 4526 4433
Size: 93 6829
BsmBI CGTCTCn'nnnn_
Cuts at: 3727 3767 3727
Size: 40 6882
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2398 2433 2530 2912 3015 3029
Size: 399 405 203 35 97 382 103 14
Cuts at: 3029 3077 3106 3229 3276 3407 3483 3555 4212
Size: 48 29 123 47 131 76 72 657
Cuts at: 4212 4241 4983 5068 6139 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 405 399 382 203
131 123 103 97 85 76 72 48
47 35 29 29 14
BspEI T'CCGG_A
Cuts at: 6585 6585
Size: 6922
BspHI T'CATG_A
Cuts at: 4811 6541 4811
Size: 1730 5192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2263 2505 2725 3435 4277 844
Size: 1419 242 220 710 842 3489
Fragments arranged by size:
3489 1419 842 710 242 220
BsrBI CCG'CTC
Cuts at: 1394 1823 2524 3370 4809 1394
Size: 429 701 846 1439 3507
Fragments arranged by size:
3507 1439 846 701 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5417 5591 65
Size: 1183 4169 174 1396
Fragments arranged by size:
4169 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2315 2436 2717 2757 2930 5566
Size: 110 228 277 121 281 40 173 2636
Cuts at: 5566 5753 1700
Size: 187 2869
Fragments arranged by size:
2869 2636 281 277 228 187 173 121
110 40
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6922
BssHII G'CGCG_C
Cuts at: 1925 2099 2386 2388 2500 2502 2504 2538 2793
Size: 174 287 2 112 2 2 34 255
Cuts at: 2793 1925
Size: 6054
Fragments arranged by size:
6054 287 255 174 112 34 2 2
2
BssSI C'ACGA_G
Cuts at: 4982 5994 4982
Size: 1012 5910
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2119 2177 3542 794
Size: 1325 58 1365 4174
Fragments arranged by size:
4174 1365 1325 58
BstBI TT'CG_AA
Cuts at: 5726 5726
Size: 6922
BstEII G'GTnAC_C
Cuts at: 1184 3457 1184
Size: 2273 4649
BstXI CCAn_nnnn'nTGG
Cuts at: 2920 2920
Size: 6922
BstYI r'GATC_y
Cuts at: 1972 2198 4252 4619 5004 5021 6473 6559 6571
Size: 226 2054 367 385 17 1452 86 12
Cuts at: 6571 6917 1972
Size: 346 1977
Fragments arranged by size:
2054 1977 1452 385 367 346 226 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2856 3126 3792 4107 2856
Size: 270 666 315 5671
Fragments arranged by size:
5671 666 315 270
BtgI C'CryG_G
Cuts at: 513 1065 2004 2247 2530 2665 3393 3981 513
Size: 552 939 243 283 135 728 588 3454
Fragments arranged by size:
3454 939 728 588 552 283 243 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6183
Fragments arranged by size:
6183 683 56
BtsI GCAGTG_nn'
Cuts at: 2342 2556 3015 3603 4438 5232 5252 2342
Size: 214 459 588 835 794 20 4012
Fragments arranged by size:
4012 835 794 588 459 214 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6922
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6887
DraI TTT'AAA
Cuts at: 4226 4226
Size: 6922
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5929 809
Size: 507 4613 1802
Fragments arranged by size:
4613 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2151 2317
Size: 54 1425 50 275 18 297 24 166
Cuts at: 2317 2352 2533 2706 3600 3627 3666 3777 3984
Size: 35 181 173 894 27 39 111 207
Cuts at: 3984 4369 5258 6887 8
Size: 385 889 1629 43
Fragments arranged by size:
1629 1425 894 889 385 297 275 207
181 173 166 111 54 50 43 39
35 27 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2127 2151 2317 2352 2533 3984 4369
Size: 275 315 24 166 35 181 1451 385
Cuts at: 4369 1537
Size: 4090
Fragments arranged by size:
4090 1451 385 315 275 181 166 35
24
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 4852 1353
Size: 723 2776 3423
Fragments arranged by size:
3423 2776 723
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2878 5509 5883 6029 387
Size: 1064 138 1289 2631 374 146 1280
Fragments arranged by size:
2631 1289 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2431 2431
Size: 6922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2885 3049 3209 3785 3877 4172
Size: 393 653 600 164 160 576 92 295
Cuts at: 4172 4985 5582 6369 6637 1239
Size: 813 597 787 268 1524
Fragments arranged by size:
1524 813 787 653 600 597 576 393
295 268 164 160 92
EcoNI CCTnn'n_nnAGG
Cuts at: 3056 3171 3257 3431 3467 3795 3056
Size: 115 86 174 36 328 6183
Fragments arranged by size:
6183 328 174 115 86 36
EcoO109I rG'GnC_Cy
Cuts at: 2191 2395 2488 2860 3025 3026 3208 3225 3265
Size: 204 93 372 165 1 182 17 40
Cuts at: 3265 3272 3273 3479 3480 3539 3730 4069 2191
Size: 7 1 206 1 59 191 339 5044
Fragments arranged by size:
5044 372 339 206 204 191 182 165
93 59 40 17 7 1 1 1
EcoRI G'AATT_C
Cuts at: 4231 4609 4231
Size: 378 6544
FspI TGC'GCA
Cuts at: 2570 5428 2570
Size: 2858 4064
HaeII r_GCGC'y
Cuts at: 1678 2297 2362 2638 2651 2701 3953 6069 1678
Size: 619 65 276 13 50 1252 2116 2531
Fragments arranged by size:
2531 2116 1252 619 276 65 50 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2825 2857 2920 2952 3185
Size: 32 135 32 1253 32 63 32 233
Cuts at: 3185 3217 3249 3281 5563 5595 5637 5669 1373
Size: 32 32 32 2282 32 42 32 2626
Fragments arranged by size:
2626 2282 1253 233 135 63 42 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4147 4266 4512 669
Size: 988 281 2209 119 246 3079
Fragments arranged by size:
3079 2209 988 281 246 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6922
HpaI GTT'AAC
Cuts at: 4512 4512
Size: 6922
KasI G'GCGC_C
Cuts at: 3949 3949
Size: 6922
KpnI G_GTAC'C
Cuts at: 3368 4247 3368
Size: 879 6043
MfeI C'AATT_G
Cuts at: 4521 4521
Size: 6922
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3183 4137 6036 6220 1305
Size: 1878 954 1899 184 2007
Fragments arranged by size:
2007 1899 1878 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2708 3629 10
Size: 54 1425 343 876 921 3303
Fragments arranged by size:
3303 1425 921 876 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 3713 3785 4880 5239 5398 518
Size: 648 1803 744 72 1095 359 159 2042
Fragments arranged by size:
2042 1803 1095 744 648 359 159 72
MspA1I CmG'CkG
Cuts at: 1606 2174 2413 2532 2584 2667 2900 2997 3079
Size: 568 239 119 52 83 233 97 82
Cuts at: 3079 3490 3530 3592 3881 3983 4338 5015 6163
Size: 411 40 62 289 102 355 677 1148
Cuts at: 6163 6408 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 568 411 355 289 245
239 233 119 102 97 83 82 62
52 40
NaeI GCC'GGC
Cuts at: 2040 2317 2040
Size: 277 6645
NarI GG'CG_CC
Cuts at: 3950 3950
Size: 6922
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5431
Fragments arranged by size:
5431 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6922
NgoMIV G'CCGG_C
Cuts at: 2038 2315 2038
Size: 277 6645
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6922
NotI GC'GGCC_GC
Cuts at: 2533 4369 2533
Size: 1836 5086
NspI r_CATG'y
Cuts at: 1132 1229 3718 4280 5825 1132
Size: 97 2489 562 1545 2229
Fragments arranged by size:
2489 2229 1545 562 97
PasI CC'CwG_GG
Cuts at: 1348 2905 3052 1348
Size: 1557 147 5218
Fragments arranged by size:
5218 1557 147
PciI A'CATG_T
Cuts at: 5821 5821
Size: 6922
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3254 3475 1108
Size: 2146 221 4555
Fragments arranged by size:
4555 2146 221
PfoI T'CCnGG_A
Cuts at: 3159 3241 3159
Size: 82 6840
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 6504 6536 4991
Size: 32 1481 32 5377
Fragments arranged by size:
5377 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2860 3265 3730 2488
Size: 372 405 465 5680
Fragments arranged by size:
5680 465 405 372
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6922
PsiI TTA'TAA
Cuts at: 4492 4492
Size: 6922
PspOMI G'GGCC_C
Cuts at: 2191 2394 2526 3025 3073 3225 3272 3479 2191
Size: 203 132 499 48 152 47 207 5634
Fragments arranged by size:
5634 499 207 203 152 132 48 47
PstI C_TGCA'G
Cuts at: 830 2487 3489 3535 3591 4274 830
Size: 1657 1002 46 56 683 3478
Fragments arranged by size:
3478 1657 1002 683 56 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
PvuII CAG'CTG
Cuts at: 3490 3530 3592 3490
Size: 40 62 6820
Fragments arranged by size:
6820 62 40
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 6922
SacI G_AGCT'C
Cuts at: 2433 2433
Size: 6922
SacII CC_GC'GG
Cuts at: 2533 2668 3984 2533
Size: 135 1316 5471
Fragments arranged by size:
5471 1316 135
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SbfI CC_TGCA'GG
Cuts at: 2487 4274 2487
Size: 1787 5135
ScaI AGT'ACT
Cuts at: 5170 6882 5170
Size: 1712 5210
SfcI C'TryA_G
Cuts at: 826 1045 2483 3485 3531 3587 4270 5405 6086
Size: 219 1438 1002 46 56 683 1135 681
Cuts at: 6086 6277 826
Size: 191 1471
Fragments arranged by size:
1471 1438 1135 1002 683 681 219 191
56 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 2316 2402 2525 2316
Size: 86 123 6713
Fragments arranged by size:
6713 123 86
SfoI GGC'GCC
Cuts at: 3951 3951
Size: 6922
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 2196 2205 2215 3330 3384 4249 1872
Size: 324 9 10 1115 54 865 4545
Fragments arranged by size:
4545 1115 865 324 54 10 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2323 2444 2651 5026 5927 6189
Size: 197 926 380 121 207 2375 901 262
Cuts at: 6189 820
Size: 1553
Fragments arranged by size:
2375 1553 926 901 380 262 207 197
121
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6922
SphI G_CATG'C
Cuts at: 1132 3718 4280 1132
Size: 2586 562 3774
Fragments arranged by size:
3774 2586 562
SspI AAT'ATT
Cuts at: 5 52 4846 5
Size: 47 4794 2081
Fragments arranged by size:
4794 2081 47
StuI AGG'CCT
Cuts at: 1517 2657 3062 3801 1517
Size: 1140 405 739 4638
Fragments arranged by size:
4638 1140 739 405
StyI C'CwwG_G
Cuts at: 513 1065 2004 3253 3844 4170 4212 4361 513
Size: 552 939 1249 591 326 42 149 3074
Fragments arranged by size:
3074 1249 939 591 552 326 149 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 4949 4966 5119 5304 5749 325
Size: 1255 513 2856 17 153 185 445 1498
Fragments arranged by size:
2856 1498 1255 513 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5168 6880 96
Size: 274 80 33 51 1443 3191 1712 138
Fragments arranged by size:
3191 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6882 6914 6882
Size: 32 6890
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6922
XbaI T'CTAG_A
Cuts at: 4258 4258
Size: 6922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3151 3290 3451 3151
Size: 139 161 6622
Fragments arranged by size:
6622 161 139
XhoI C'TCGA_G
Cuts at: 1943 2444 1943
Size: 501 6421
XmaI C'CCGG_G
Cuts at: 1870 2194 2203 2213 3328 3382 4247 1870
Size: 324 9 10 1115 54 865 4545
Fragments arranged by size:
4545 1115 865 324 54 10 9
XmnI GAAnn'nnTTC
Cuts at: 5051 5051
Size: 6922
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI SanDI SapI SexAI SrfI SwaI