Restriction map for
pFN21AB9758
(Circular) (Six-base) MAPSORT of: pFN21AB9758.seq Check: 8626 from: 1 to: 6838
pFN21AB9758 6838 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2846 3068 3187 2846
Size: 222 119 6497
Fragments arranged by size:
6497 222 119
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2235 2286 4181 1937
Size: 298 51 1895 4594
Fragments arranged by size:
4594 1895 298 51
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2568 3023 3113 4901 6285 6553 1632
Size: 936 455 90 1788 1384 268 1917
Fragments arranged by size:
1917 1788 1384 936 455 268 90
AfeI AGC'GCT
Cuts at: 3267 3267
Size: 6838
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 3488 5737 6714 1203
Size: 2285 2249 977 1327
Fragments arranged by size:
2285 2249 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 2094 2526 3038 3462 4031 1166
Size: 928 432 512 424 569 3973
Fragments arranged by size:
3973 928 569 512 432 424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2769 1184
Size: 1585 5253
AlwNI CAG_nnn'CTG
Cuts at: 2861 4581 6153 2861
Size: 1720 1572 3546
Fragments arranged by size:
3546 1720 1572
ApaLI G'TGCA_C
Cuts at: 3113 4895 6051 3113
Size: 1782 1156 3900
Fragments arranged by size:
3900 1782 1156
ApoI r'AATT_y
Cuts at: 1493 2428 3369 3598 4147 4373 4525 6634 1493
Size: 935 941 229 549 226 152 2109 1697
Fragments arranged by size:
2109 1697 941 935 549 229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 4163 1870
Size: 73 2220 4545
Fragments arranged by size:
4545 2220 73
BamHI G'GATC_C
Cuts at: 3548 4168 4535 3548
Size: 620 367 5851
Fragments arranged by size:
5851 620 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3222 3326 4159 5614 618
Size: 325 217 627 1435 104 833 1455 1842
Fragments arranged by size:
1842 1455 1435 833 627 325 217 104
BanII G_rGCy'C
Cuts at: 1391 4157 1391
Size: 2766 4072
BbeI G_GCGC'C
Cuts at: 3330 3330
Size: 6838
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2574 2737 928
Size: 971 675 163 5029
Fragments arranged by size:
5029 971 675 163
BbvCI CC'TCA_GC
Cuts at: 2757 2757
Size: 6838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2057 2091 5029 5063 1903
Size: 34 120 34 2938 34 3678
Fragments arranged by size:
3678 2938 120 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4730 5940 4730
Size: 1210 5628
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6838
BfrBI ATG'CAT
Cuts at: 2068 2068
Size: 6838
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2153 2495 3240
Size: 107 122 71 631 605 481 342 745
Cuts at: 3240 4016 5449 136
Size: 776 1433 1525
Fragments arranged by size:
1525 1433 776 745 631 605 481 342
122 107 71
BglII A'GATC_T
Cuts at: 6833 6833
Size: 6838
BlpI GC'TnA_GC
Cuts at: 4073 4255 4073
Size: 182 6656
Bme1580I G_kGCm'C
Cuts at: 1790 2816 3117 4899 6055 1790
Size: 1026 301 1782 1156 2573
Fragments arranged by size:
2573 1782 1156 1026 301
BmgBI CAC'GTC
Cuts at: 1318 2101 3178 1318
Size: 783 1077 4978
Fragments arranged by size:
4978 1077 783
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2520 4569 5527
Size: 416 53 83 399 24 1103 2049 958
Cuts at: 5527 442
Size: 1753
Fragments arranged by size:
2049 1753 1103 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3674 5498 1239
Size: 2435 1824 2579
Fragments arranged by size:
2579 2435 1824
Bpu10I CC'TnA_GC
Cuts at: 2757 2757
Size: 6838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 3338 4963 5828 6126 2297
Size: 1041 1625 865 298 3009
Fragments arranged by size:
3009 1625 1041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2537 3291 5501 882
Size: 617 1038 754 2210 2219
Fragments arranged by size:
2219 2210 1038 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 3112 3190 4629 493
Size: 711 1908 78 1439 2702
Fragments arranged by size:
2702 1908 1439 711 78
BsaBI GATnn'nnATC
Cuts at: 6832 6832
Size: 6838
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3327 4645 5027 5662
Size: 53 83 186 936 1794 1318 382 635
Cuts at: 5662 275
Size: 1451
Fragments arranged by size:
1794 1451 1318 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5271 5669 5943 6090 6501 5271
Size: 398 274 147 411 5608
Fragments arranged by size:
5608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2656 2686 2861 2891 1178
Size: 30 165 30 1253 30 175 30 5125
Fragments arranged by size:
5125 1253 175 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2912 2921 2924 2927 2930 2933 1186
Size: 1726 9 3 3 3 3 5091
Fragments arranged by size:
5091 1726 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2684 3591 3675 6041 6537 2684
Size: 907 84 2366 496 2985
Fragments arranged by size:
2985 2366 907 496 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2037 2270 2342 2980 3260 3298 1163
Size: 573 301 233 72 638 280 38 4703
Fragments arranged by size:
4703 638 573 301 280 233 72 38
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3117 4899 4984 6055 3117
Size: 1782 85 1071 3900
Fragments arranged by size:
3900 1782 1071 85
BsmI GAATG_Cn'
Cuts at: 2334 4349 4442 2334
Size: 2015 93 4730
Fragments arranged by size:
4730 2015 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2816 3117 4157 4899 4984 6055 1391
Size: 399 1026 301 1040 742 85 1071 2174
Fragments arranged by size:
2174 1071 1040 1026 742 399 301 85
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 4727 6457 4727
Size: 1730 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2242 2818 2846 3068 3187 4193 844
Size: 1398 576 28 222 119 1006 3489
Fragments arranged by size:
3489 1398 1006 576 222 119 28
BsrBI CCG'CTC
Cuts at: 1394 1823 3220 4725 1394
Size: 429 1397 1505 3507
Fragments arranged by size:
3507 1505 1397 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2578 3742 5333 5507 65
Size: 1183 1330 1164 1591 174 1396
Fragments arranged by size:
1591 1396 1330 1183 1164 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3300 5482 5669 1700
Size: 110 1490 2182 187 2869
Fragments arranged by size:
2869 2182 1490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 3278 4898 5910 3278
Size: 1620 1012 4206
Fragments arranged by size:
4206 1620 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2065 2329 2967 3193 3321 3741 794
Size: 1271 264 638 226 128 420 3891
Fragments arranged by size:
3891 1271 638 420 264 226 128
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 2087 1184
Size: 903 5935
BstXI CCAn_nnnn'nTGG
Cuts at: 3040 3040
Size: 6838
BstYI r'GATC_y
Cuts at: 1972 2706 3548 4168 4535 4920 4937 6389 6475
Size: 734 842 620 367 385 17 1452 86
Cuts at: 6475 6487 6833 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 842 734 620 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3022 3809 4029 513
Size: 552 939 1018 787 220 3322
Fragments arranged by size:
3322 1018 939 787 552 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 2289 2467 4354 5148 5168 2163
Size: 126 178 1887 794 20 3833
Fragments arranged by size:
3833 1887 794 178 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 3562 4142 3562
Size: 580 6258
DraIII CAC_nnn'GTG
Cuts at: 3028 3321 3028
Size: 293 6545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2105 5845 809
Size: 507 789 3740 1802
Fragments arranged by size:
3740 1802 789 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 3030 3064
Size: 54 1425 50 275 18 675 525 34
Cuts at: 3064 3232 3937 4053 4285 5174 6803 8
Size: 168 705 116 232 889 1629 43
Fragments arranged by size:
1629 1425 889 705 675 525 275 232
168 116 54 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2898 2939 2987 3089 3777 3879 4768
Size: 848 697 41 48 102 688 102 889
Cuts at: 4768 1353
Size: 3423
Fragments arranged by size:
3423 889 848 697 688 102 102 48
41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2568 3023 3113 3674 4901 5498 6285
Size: 393 936 455 90 561 1227 597 787
Cuts at: 6285 6553 1239
Size: 268 1524
Fragments arranged by size:
1524 1227 936 787 597 561 455 393
268 90
EcoO109I rG'GnC_Cy
Cuts at: 3247 3247
Size: 6838
EcoRI G'AATT_C
Cuts at: 3369 3598 4147 4525 3369
Size: 229 549 378 5682
Fragments arranged by size:
5682 549 378 229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2213 2968 3535 2213
Size: 755 567 5516
Fragments arranged by size:
5516 755 567
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 3109 3130 3269 3330 3697 5985 1678
Size: 1431 21 139 61 367 2288 2531
Fragments arranged by size:
2531 2288 1431 367 139 61 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2861 2893 3878 3910 5479
Size: 32 135 32 1289 32 985 32 1569
Cuts at: 5479 5511 5553 5585 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1569 1289 985 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4182 4428 669
Size: 988 281 2244 246 3079
Fragments arranged by size:
3079 2244 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3902 748
Size: 3154 3684
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KasI G'GCGC_C
Cuts at: 3326 3326
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 4437 4437
Size: 6838
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2847 5952 6136 1305
Size: 1542 3105 184 2007
Fragments arranged by size:
3105 2007 1542 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2507 3032 3939 4055 10
Size: 54 1425 343 675 525 907 116 2793
Fragments arranged by size:
2793 1425 907 675 525 343 116 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 2526 2819 3038 3462 3730 4031
Size: 648 928 432 293 219 424 268 301
Cuts at: 4031 4796 5155 5314 518
Size: 765 359 159 2042
Fragments arranged by size:
2042 928 765 648 432 424 359 301
293 268 219 159
MspA1I CmG'CkG
Cuts at: 1606 3051 3084 3228 3595 4254 4931 6079 6324
Size: 1445 33 144 367 659 677 1148 245
Cuts at: 6324 1606
Size: 2120
Fragments arranged by size:
2120 1445 1148 677 659 367 245 144
33
NarI GG'CG_CC
Cuts at: 3327 3327
Size: 6838
NcoI C'CATG_G
Cuts at: 513 1065 2004 3809 513
Size: 552 939 1805 3542
Fragments arranged by size:
3542 1805 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NsiI A_TGCA'T
Cuts at: 2070 2070
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 2581 3436 3492 4196 5741 1132
Size: 97 1352 855 56 704 1545 2229
Fragments arranged by size:
2229 1545 1352 855 704 97 56
PasI CC'CwG_GG
Cuts at: 1348 2493 1348
Size: 1145 5693
PciI A'CATG_T
Cuts at: 3488 5737 3488
Size: 2249 4589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3028 3952 1108
Size: 1920 924 3994
Fragments arranged by size:
3994 1920 924
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PmlI CAC'GTG
Cuts at: 3112 3190 3112
Size: 78 6760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2231 1613
Size: 618 6220
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PstI C_TGCA'G
Cuts at: 830 3198 3961 4190 830
Size: 2368 763 229 3478
Fragments arranged by size:
3478 2368 763 229
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SapI GCTCTTCn'nnn_
Cuts at: 2939 2987 3089 2939
Size: 48 102 6688
Fragments arranged by size:
6688 102 48
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 2412 3518 5086 6798 2412
Size: 1106 1568 1712 2452
Fragments arranged by size:
2452 1712 1568 1106
SfcI C'TryA_G
Cuts at: 826 1045 2776 3194 3957 4186 5321 6002 6193
Size: 219 1731 418 763 229 1135 681 191
Cuts at: 6193 826
Size: 1471
Fragments arranged by size:
1731 1471 1135 763 681 418 229 219
191
SfoI GGC'GCC
Cuts at: 3328 3328
Size: 6838
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 4165 1872
Size: 2293 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 3353 4942 5843 6105 820
Size: 197 926 333 1077 1589 901 262 1553
Fragments arranged by size:
1589 1553 1077 926 901 333 262 197
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 4196 1132
Size: 3064 3774
SspI AAT'ATT
Cuts at: 5 52 4762 5
Size: 47 4710 2081
Fragments arranged by size:
4710 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6838
StyI C'CwwG_G
Cuts at: 513 1065 2004 2544 2607 3809 4277 513
Size: 552 939 540 63 1202 468 3074
Fragments arranged by size:
3074 1202 939 552 540 468 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3689 4865 4882 5035 5220 5665 325
Size: 1255 2109 1176 17 153 185 445 1498
Fragments arranged by size:
2109 1498 1255 1176 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2410 3516 5084
Size: 274 80 33 51 1443 433 1106 1568
Cuts at: 5084 6796 96
Size: 1712 138
Fragments arranged by size:
1712 1568 1443 1106 433 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2656 2688 2765 2797 6798 6830 2656
Size: 32 77 32 4001 32 2664
Fragments arranged by size:
4001 2664 77 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2500 2500
Size: 6838
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 4163 1870
Size: 2293 4545
XmnI GAAnn'nnTTC
Cuts at: 2996 3158 3818 4967 2996
Size: 162 660 1149 4867
Fragments arranged by size:
4867 1149 660 162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI