Restriction map for
pFN21AB9749
(Circular) (Six-base) MAPSORT of: pFN21AB9749.seq Check: 7219 from: 1 to: 6382
pFN21AB9749 6382 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2361 2361
Size: 6382
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3725 1937
Size: 1788 4594
Acc65I G'GTAC_C
Cuts at: 3703 3703
Size: 6382
AclI AA'CG_TT
Cuts at: 1563 4509 4882 1563
Size: 2946 373 3063
Fragments arranged by size:
3063 2946 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3304 4445 5829 6097 1632
Size: 1672 1141 1384 268 1917
Fragments arranged by size:
1917 1672 1384 1141 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6185
AflIII A'CryG_T
Cuts at: 1203 2194 5281 6258 1203
Size: 991 3087 977 1327
Fragments arranged by size:
3087 1327 991 977
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 2455 3232 3262 5111 2455
Size: 777 30 1849 3726
Fragments arranged by size:
3726 1849 777 30
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3563 1184
Size: 2379 4003
AlwNI CAG_nnn'CTG
Cuts at: 4125 5697 4125
Size: 1572 4810
ApaI G_GGCC'C
Cuts at: 2820 2820
Size: 6382
ApaLI G'TGCA_C
Cuts at: 2017 2264 4439 5595 2017
Size: 247 2175 1156 2804
Fragments arranged by size:
2804 2175 1156 247
ApoI r'AATT_y
Cuts at: 1493 3691 3917 4069 6178 1493
Size: 2198 226 152 2109 1697
Fragments arranged by size:
2198 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4936 160
Size: 872 3904 1606
Fragments arranged by size:
3904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2844 3045 3295 3431 3707 1870
Size: 73 177 724 201 250 136 276 4545
Fragments arranged by size:
4545 724 276 250 201 177 136 73
BamHI G'GATC_C
Cuts at: 3712 4079 3712
Size: 367 6015
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 2942 3470 3703 5158
Size: 325 217 627 947 208 528 233 1455
Cuts at: 5158 618
Size: 1842
Fragments arranged by size:
1842 1455 947 627 528 325 233 217
208
BanII G_rGCy'C
Cuts at: 1391 2211 2645 2820 3045 3375 3431 3701 1391
Size: 820 434 175 225 330 56 270 4072
Fragments arranged by size:
4072 820 434 330 270 225 175 56
BbeI G_GCGC'C
Cuts at: 2738 2738
Size: 6382
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2152 2531 928
Size: 971 253 379 4779
Fragments arranged by size:
4779 971 379 253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3260 3294 4573 4607 1903
Size: 34 1323 34 1279 34 3678
Fragments arranged by size:
3678 1323 1279 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4274 5484 4274
Size: 1210 5172
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6382
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3059 4993 136
Size: 107 122 71 631 605 1387 1934 1525
Fragments arranged by size:
1934 1525 1387 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6377 6377
Size: 6382
BlpI GC'TnA_GC
Cuts at: 3799 3799
Size: 6382
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2268 2820 3473 4443 5599 1790
Size: 231 247 552 653 970 1156 2573
Fragments arranged by size:
2573 1156 970 653 552 247 231
BmgBI CAC'GTC
Cuts at: 1318 2802 1318
Size: 1484 4898
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2460 3115 4113
Size: 416 53 83 399 24 1043 655 998
Cuts at: 4113 5071 442
Size: 958 1753
Fragments arranged by size:
1753 1043 998 958 655 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3100 3274 3100
Size: 174 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2678 2922 3086 3124 3130 3142 3292 3686
Size: 1439 244 164 38 6 12 150 394
Cuts at: 3686 5042 1239
Size: 1356 2579
Fragments arranged by size:
2579 1439 1356 394 244 164 150 38
12 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3446 4507 5372 5670 3446
Size: 1061 865 298 4158
Fragments arranged by size:
4158 1061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2777 3629 5045 882
Size: 617 1278 852 1416 2219
Fragments arranged by size:
2219 1416 1278 852 617
BsaAI yAC'GTr
Cuts at: 493 1204 4173 493
Size: 711 2969 2702
Fragments arranged by size:
2969 2702 711
BsaBI GATnn'nnATC
Cuts at: 6376 6376
Size: 6382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2553 2735 4189 4571
Size: 53 83 186 936 1020 182 1454 382
Cuts at: 4571 5206 275
Size: 635 1451
Fragments arranged by size:
1454 1451 1020 936 635 382 186 182
83 53
BsaWI w'CCGG_w
Cuts at: 2397 3513 4815 5213 5487 5634 6045 2397
Size: 1116 1302 398 274 147 411 2734
Fragments arranged by size:
2734 1302 1116 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 2384 2414 3083
Size: 30 165 30 816 30 135 30 669
Cuts at: 3083 3113 3128 3158 3608 3638 1178
Size: 30 15 30 450 30 3922
Fragments arranged by size:
3922 816 669 450 165 135 30 30
30 30 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2297 2635 2741 2950 2953 2956 2959
Size: 1054 57 338 106 209 3 3 3
Cuts at: 2959 3089 3254 3287 3343 3439 3587 1186
Size: 130 165 33 56 96 148 3981
Fragments arranged by size:
3981 1054 338 209 165 148 130 106
96 57 56 33 3 3 3
BseYI C'CCAG_C
Cuts at: 2041 2701 2888 3362 5585 6081 2041
Size: 660 187 474 2223 496 2342
Fragments arranged by size:
2342 2223 660 496 474 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 5392
Fragments arranged by size:
5392 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2864 3832 4593 5621 1540
Size: 275 186 863 968 761 1028 2301
Fragments arranged by size:
2301 1028 968 863 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 2268 2607 2645 2938 4443 4528 5599 2021
Size: 247 339 38 293 1505 85 1071 2804
Fragments arranged by size:
2804 1505 1071 339 293 247 85 38
BsmI GAATG_Cn'
Cuts at: 3893 3986 3893
Size: 93 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2211 2268 2607 2645 2820 2938
Size: 399 231 190 57 339 38 175 118
Cuts at: 2938 3045 3375 3431 3473 3701 4443 4528 5599
Size: 107 330 56 42 228 742 85 1071
Cuts at: 5599 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 339 330 231 228
190 175 118 107 85 57 56 42
38
BspEI T'CCGG_A
Cuts at: 6045 6045
Size: 6382
BspHI T'CATG_A
Cuts at: 4271 6001 4271
Size: 1730 4652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 3237 3737 844
Size: 1517 876 500 3489
Fragments arranged by size:
3489 1517 876 500
BsrBI CCG'CTC
Cuts at: 1394 1823 2614 4269 1394
Size: 429 791 1655 3507
Fragments arranged by size:
3507 1655 791 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2132 4877 5051 65
Size: 1183 884 2745 174 1396
Fragments arranged by size:
2745 1396 1183 884 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2417 2712 2863 2989 3281 5026 5213
Size: 110 607 295 151 126 292 1745 187
Cuts at: 5213 1700
Size: 2869
Fragments arranged by size:
2869 1745 607 295 292 187 151 126
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6382
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 4442 5454 4442
Size: 1012 5370
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6382
BstBI TT'CG_AA
Cuts at: 5186 5186
Size: 6382
BstEII G'GTnAC_C
Cuts at: 1184 2593 1184
Size: 1409 4973
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2656
Size: 6382
BstYI r'GATC_y
Cuts at: 1972 3456 3712 4079 4464 4481 5933 6019 6031
Size: 1484 256 367 385 17 1452 86 12
Cuts at: 6031 6377 1972
Size: 346 1977
Fragments arranged by size:
1977 1484 1452 385 367 346 256 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 2113 2388 2539 3216 3672
Size: 552 939 72 37 275 151 677 456
Cuts at: 3672 513
Size: 3223
Fragments arranged by size:
3223 939 677 552 456 275 151 72
37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2033 487
Size: 683 56 807 4836
Fragments arranged by size:
4836 807 683 56
BtsI GCAGTG_nn'
Cuts at: 3898 4692 4712 3898
Size: 794 20 5568
Fragments arranged by size:
5568 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6347
DraI TTT'AAA
Cuts at: 3686 3686
Size: 6382
DraIII CAC_nnn'GTG
Cuts at: 2146 2593 2146
Size: 447 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5389 809
Size: 507 4073 1802
Fragments arranged by size:
4073 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2861 2865 2991
Size: 54 1425 50 275 18 1031 4 126
Cuts at: 2991 3580 3675 3829 4718 6347 8
Size: 589 95 154 889 1629 43
Fragments arranged by size:
1629 1425 1031 889 589 275 154 126
95 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2861 3829 1537
Size: 275 1049 968 4090
Fragments arranged by size:
4090 1049 968 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2903 3265 3626 4312 1353
Size: 1550 362 361 686 3423
Fragments arranged by size:
3423 1550 686 362 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4969 5343 5489 387
Size: 1064 138 3380 374 146 1280
Fragments arranged by size:
3380 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2643 2643
Size: 6382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2678 2922 3086 3124 3130 3142 3292
Size: 393 1046 244 164 38 6 12 150
Cuts at: 3292 3304 3686 4445 5042 5829 6097 1239
Size: 12 382 759 597 787 268 1524
Fragments arranged by size:
1524 1046 787 759 597 393 382 268
244 164 150 38 12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3464 3464
Size: 6382
EcoO109I rG'GnC_Cy
Cuts at: 2817 2925 3061 3203 3226 2817
Size: 108 136 142 23 5973
Fragments arranged by size:
5973 142 136 108 23
EcoRI G'AATT_C
Cuts at: 3691 4069 3691
Size: 378 6004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2317 2389 2770 2176
Size: 141 72 381 5788
Fragments arranged by size:
5788 381 141 72
FseI GG_CCGG'CC
Cuts at: 2867 2867
Size: 6382
FspI TGC'GCA
Cuts at: 2063 3647 4888 2063
Size: 1584 1241 3557
Fragments arranged by size:
3557 1584 1241
HaeII r_GCGC'y
Cuts at: 1678 2738 2895 3348 3530 5529 1678
Size: 1060 157 453 182 1999 2531
Fragments arranged by size:
2531 1999 1060 453 182 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 3083 3115 3146
Size: 32 135 32 869 32 610 32 31
Cuts at: 3146 3178 3218 3248 3250 3280 3547 3579 3608
Size: 32 40 30 2 30 267 32 29
Cuts at: 3608 3640 5023 5055 5097 5129 1373
Size: 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 869 610 267 135 42 40
32 32 32 32 32 32 32 32
32 31 30 30 29 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3726 3972 669
Size: 988 281 1788 246 3079
Fragments arranged by size:
3079 1788 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6382
HpaI GTT'AAC
Cuts at: 3972 3972
Size: 6382
KasI G'GCGC_C
Cuts at: 2734 2734
Size: 6382
KpnI G_GTAC'C
Cuts at: 3707 3707
Size: 6382
MfeI C'AATT_G
Cuts at: 3981 3981
Size: 6382
MluI A'CGCG_T
Cuts at: 2194 6258 2194
Size: 4064 2318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3105 3159 3270 5496 5680 1305
Size: 1800 54 111 2226 184 2007
Fragments arranged by size:
2226 2007 1800 184 111 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3582 3677 10
Size: 54 1425 343 1750 95 2715
Fragments arranged by size:
2715 1750 1425 343 95 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 3588 4340 4699 4858 518
Size: 648 1095 1327 752 359 159 2042
Fragments arranged by size:
2042 1327 1095 752 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2115 3798 4475 5623 5868 1606
Size: 509 1683 677 1148 245 2120
Fragments arranged by size:
2120 1683 1148 677 509 245
NaeI GCC'GGC
Cuts at: 2419 2714 2865 2991 3283 2419
Size: 295 151 126 292 5518
Fragments arranged by size:
5518 295 292 151 126
NarI GG'CG_CC
Cuts at: 2735 2735
Size: 6382
NcoI C'CATG_G
Cuts at: 513 1065 2004 3216 513
Size: 552 939 1212 3679
Fragments arranged by size:
3679 1212 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6382
NgoMIV G'CCGG_C
Cuts at: 2417 2712 2863 2989 3281 2417
Size: 295 151 126 292 5518
Fragments arranged by size:
5518 295 292 151 126
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NspI r_CATG'y
Cuts at: 1132 1229 3740 5285 1132
Size: 97 2511 1545 2229
Fragments arranged by size:
2511 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6382
PciI A'CATG_T
Cuts at: 5281 5281
Size: 6382
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6382
PfoI T'CCnGG_A
Cuts at: 2927 2927
Size: 6382
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4451 4483 5964 5996 4451
Size: 32 1481 32 4837
Fragments arranged by size:
4837 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2925 3203 3226 2925
Size: 278 23 6081
Fragments arranged by size:
6081 278 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6382
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PspOMI G'GGCC_C
Cuts at: 2816 2816
Size: 6382
PstI C_TGCA'G
Cuts at: 830 3234 3443 3552 3657 3734 830
Size: 2404 209 109 105 77 3478
Fragments arranged by size:
3478 2404 209 109 105 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
SacI G_AGCT'C
Cuts at: 2645 2645
Size: 6382
SacII CC_GC'GG
Cuts at: 2116 2116
Size: 6382
SalI G'TCGA_C
Cuts at: 1936 3724 1936
Size: 1788 4594
SanDI GG'GwC_CC
Cuts at: 2925 2925
Size: 6382
SapI GCTCTTCn'nnn_
Cuts at: 2903 2903
Size: 6382
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 4630 6342 4630
Size: 1712 4670
SfcI C'TryA_G
Cuts at: 826 1045 3230 3439 3548 3653 3730 4865 5546
Size: 219 2185 209 109 105 77 1135 681
Cuts at: 5546 5737 826
Size: 191 1471
Fragments arranged by size:
2185 1471 1135 681 219 209 191 109
105 77
SfiI GGCCn_nnn'nGGCC
Cuts at: 3059 3059
Size: 6382
SfoI GGC'GCC
Cuts at: 2736 2736
Size: 6382
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 2846 3047 3297 3709 1872
Size: 974 201 250 412 4545
Fragments arranged by size:
4545 974 412 250 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3461 4486 5387 5649 820
Size: 197 926 1518 1025 901 262 1553
Fragments arranged by size:
1553 1518 1025 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6382
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SspI AAT'ATT
Cuts at: 5 52 4306 5
Size: 47 4254 2081
Fragments arranged by size:
4254 2081 47
StuI AGG'CCT
Cuts at: 1517 3098 3485 1517
Size: 1581 387 4414
Fragments arranged by size:
4414 1581 387
StyI C'CwwG_G
Cuts at: 513 1065 2004 2547 2598 2649 2682 2721 2745
Size: 552 939 543 51 51 33 39 24
Cuts at: 2745 2808 2994 3199 3216 3821 513
Size: 63 186 205 17 605 3074
Fragments arranged by size:
3074 939 605 552 543 205 186 63
51 51 39 33 24 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4409 4426 4579 4764 5209 325
Size: 1255 2829 17 153 185 445 1498
Fragments arranged by size:
2829 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4628 6340 96
Size: 274 80 33 51 1443 2651 1712 138
Fragments arranged by size:
2651 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 6342 6374 2000
Size: 32 4310 32 2008
Fragments arranged by size:
4310 2008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6382
XbaI T'CTAG_A
Cuts at: 3718 3718
Size: 6382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3061 3061
Size: 6382
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6382
XmaI C'CCGG_G
Cuts at: 1870 2844 3045 3295 3707 1870
Size: 974 201 250 412 4545
Fragments arranged by size:
4545 974 412 250 201
XmnI GAAnn'nnTTC
Cuts at: 4511 4511
Size: 6382
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SexAI SrfI
SwaI