Restriction map for
pFN21AB9743
(Circular) (Six-base) MAPSORT of: pFN21AB9743.seq Check: 7060 from: 1 to: 6277
pFN21AB9743 6277 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2442 2442
Size: 6277
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3620 1937
Size: 1683 4594
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 3576 4404 4777 1563
Size: 2013 828 373 3063
Fragments arranged by size:
3063 2013 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2774 4340 5724 5992 1632
Size: 422 720 1566 1384 268 1917
Fragments arranged by size:
1917 1566 1384 720 422 268
AfeI AGC'GCT
Cuts at: 2822 2912 2822
Size: 90 6187
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 5176 6153 1203
Size: 3973 977 1327
Fragments arranged by size:
3973 1327 977
AgeI A'CCGG_T
Cuts at: 2766 5108 2766
Size: 2342 3935
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 2778 1166
Size: 1612 4665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2454 1184
Size: 1270 5007
AlwNI CAG_nnn'CTG
Cuts at: 2362 4020 5592 2362
Size: 1658 1572 3047
Fragments arranged by size:
3047 1658 1572
ApaI G_GGCC'C
Cuts at: 2428 3058 3203 3370 2428
Size: 630 145 167 5335
Fragments arranged by size:
5335 630 167 145
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 3586 3812 3964 6073 1493
Size: 2093 226 152 2109 1697
Fragments arranged by size:
2109 2093 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4831 160
Size: 872 3799 1606
Fragments arranged by size:
3799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 2201 2303 2591 3602 1870
Size: 73 258 102 288 1011 4545
Fragments arranged by size:
4545 1011 288 258 102 73
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3355 3458 3598 5053 618
Size: 325 217 627 1568 103 140 1455 1842
Fragments arranged by size:
1842 1568 1455 627 325 217 140 103
BanII G_rGCy'C
Cuts at: 1391 2428 2547 2988 3058 3203 3370 3548 3596
Size: 1037 119 441 70 145 167 178 48
Cuts at: 3596 1391
Size: 4072
Fragments arranged by size:
4072 1037 441 178 167 145 119 70
48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5306
BbvCI CC'TCA_GC
Cuts at: 2246 2246
Size: 6277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2582 2616 4468 4502 1903
Size: 34 50 34 561 34 1852 34 3678
Fragments arranged by size:
3678 1852 561 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4169 5379 4169
Size: 1210 5067
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3108 4888 136
Size: 107 122 71 631 605 1436 1780 1525
Fragments arranged by size:
1780 1525 1436 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 2340 2428 3058 3154 3203 3360 3370 4338
Size: 550 88 630 96 49 157 10 968
Cuts at: 4338 5494 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 968 630 550 157 96 88
49 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 2629 3290
Size: 416 53 83 399 24 917 295 661
Cuts at: 3290 4008 4966 442
Size: 718 958 1753
Fragments arranged by size:
1753 958 917 718 661 416 399 295
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2316 2765 2818 4937 1239
Size: 903 174 449 53 2119 2579
Fragments arranged by size:
2579 2119 903 449 174 53
Bpu10I CC'TnA_GC
Cuts at: 2246 2246
Size: 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4402 5267 5565 4402
Size: 865 298 5114
Fragments arranged by size:
5114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3301 4940 882
Size: 617 1802 1639 2219
Fragments arranged by size:
2219 1802 1639 617
BsaAI yAC'GTr
Cuts at: 493 1204 2869 4068 493
Size: 711 1665 1199 2702
Fragments arranged by size:
2702 1665 1199 711
BsaBI GATnn'nnATC
Cuts at: 2672 3065 6271 2672
Size: 393 3206 2678
Fragments arranged by size:
3206 2678 393
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3392 3483 4084 4466
Size: 53 83 186 936 1859 91 601 382
Cuts at: 4466 5101 275
Size: 635 1451
Fragments arranged by size:
1859 1451 936 635 601 382 186 91
83 53
BsaWI w'CCGG_w
Cuts at: 2766 4710 5108 5382 5529 5940 2766
Size: 1944 398 274 147 411 3103
Fragments arranged by size:
3103 1944 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6052
Fragments arranged by size:
6052 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2434 1186
Size: 1248 5029
BseYI C'CCAG_C
Cuts at: 2086 2122 2384 2517 2924 3076 3525 5480 5976
Size: 36 262 133 407 152 449 1955 496
Cuts at: 5976 2086
Size: 2387
Fragments arranged by size:
2387 1955 496 449 407 262 152 133
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 2414 1163
Size: 573 360 318 5026
Fragments arranged by size:
5026 573 360 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2771 3727 4488 5516 1540
Size: 275 186 770 956 761 1028 2301
Fragments arranged by size:
2301 1028 956 770 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2110 2547 2988 4338 4423 5494 2110
Size: 437 441 1350 85 1071 2893
Fragments arranged by size:
2893 1350 1071 441 437 85
BsmI GAATG_Cn'
Cuts at: 3788 3881 3788
Size: 93 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2340 2428 2547 2988 3058 3154
Size: 399 320 230 88 119 441 70 96
Cuts at: 3154 3203 3360 3370 3548 3596 4338 4423 5494
Size: 49 157 10 178 48 742 85 1071
Cuts at: 5494 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 441 399 320 230 178
157 119 96 88 85 70 49 48
10
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 4166 5896 4166
Size: 1730 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 2442 2496 2729 3632 844
Size: 1490 108 54 233 903 3489
Fragments arranged by size:
3489 1490 903 233 108 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 4164 1394
Size: 429 203 2138 3507
Fragments arranged by size:
3507 2138 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 4772 4946 65
Size: 1183 3524 174 1396
Fragments arranged by size:
3524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2766 3562 4921 5108 1700
Size: 110 956 796 1359 187 2869
Fragments arranged by size:
2869 1359 956 796 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 2237 2972 4337 5349 2237
Size: 735 1365 1012 3165
Fragments arranged by size:
3165 1365 1012 735
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6277
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6277
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 3370 2506
Size: 864 5413
BstYI r'GATC_y
Cuts at: 1972 2757 3607 3974 4359 4376 5828 5914 5926
Size: 785 850 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 850 785 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2173 2173
Size: 6277
BtgI C'CryG_G
Cuts at: 513 1065 2638 2953 3477 513
Size: 552 1573 315 524 3313
Fragments arranged by size:
3313 1573 552 524 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 2360 2930 3017 3184 3793 4587 4607 2360
Size: 570 87 167 609 794 20 4030
Fragments arranged by size:
4030 794 609 570 167 87 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DraIII CAC_nnn'GTG
Cuts at: 2869 2965 2869
Size: 96 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5284 809
Size: 507 3968 1802
Fragments arranged by size:
3968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2641 2956 3237
Size: 54 1425 50 275 18 811 315 281
Cuts at: 3237 3425 3724 4613 6242 8
Size: 188 299 889 1629 43
Fragments arranged by size:
1629 1425 889 811 315 299 281 275
188 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4207 1353
Size: 2854 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3291 4864 5238 5384 387
Size: 1064 138 1702 1573 374 146 1280
Fragments arranged by size:
1702 1573 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2545 2986 2545
Size: 441 5836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2142 2316 2765 2774 2818 4340
Size: 393 422 88 174 449 9 44 1522
Cuts at: 4340 4937 5724 5992 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1522 787 597 449 422 393 268
174 88 44 9
EcoO109I rG'GnC_Cy
Cuts at: 2323 2424 2425 3054 3095 3200 3215 3366 3367
Size: 101 1 629 41 105 15 151 1
Cuts at: 3367 2323
Size: 5233
Fragments arranged by size:
5233 629 151 105 101 41 15 1
1
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FspI TGC'GCA
Cuts at: 2936 4783 2936
Size: 1847 4430
HaeII r_GCGC'y
Cuts at: 1678 2311 2824 2914 5424 1678
Size: 633 513 90 2510 2531
Fragments arranged by size:
2531 2510 633 513 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2912 2944 4918 4950 4992
Size: 32 135 32 1340 32 1974 32 42
Cuts at: 4992 5024 1373
Size: 32 2626
Fragments arranged by size:
2626 1974 1340 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2572 3487 3621 3867 669
Size: 988 281 634 915 134 246 3079
Fragments arranged by size:
3079 988 915 634 281 246 134
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6277
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2741 2853 5391 5575 1305
Size: 1436 112 2538 184 2007
Fragments arranged by size:
2538 2007 1436 184 112
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2958 3239 10
Size: 54 1425 343 1126 281 3048
Fragments arranged by size:
3048 1425 1126 343 281 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2778 4235 4594 4753 518
Size: 648 1612 1457 359 159 2042
Fragments arranged by size:
2042 1612 1457 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2506 2712 2995 3080 3250 3317 3417 3494
Size: 900 206 283 85 170 67 100 77
Cuts at: 3494 3693 4370 5518 5763 1606
Size: 199 677 1148 245 2120
Fragments arranged by size:
2120 1148 900 677 283 245 206 199
170 100 85 77 67
NcoI C'CATG_G
Cuts at: 513 1065 2638 2953 513
Size: 552 1573 315 3837
Fragments arranged by size:
3837 1573 552 315
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 3635 5180 1132
Size: 97 2406 1545 2229
Fragments arranged by size:
2406 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3203 3370 1348
Size: 1855 167 4255
Fragments arranged by size:
4255 1855 167
PciI A'CATG_T
Cuts at: 5176 5176
Size: 6277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2264 2869 1108
Size: 1156 605 4516
Fragments arranged by size:
4516 1156 605
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 2323
Size: 6277
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PspOMI G'GGCC_C
Cuts at: 2424 3054 3199 3366 2424
Size: 630 145 167 5335
Fragments arranged by size:
5335 630 167 145
PstI C_TGCA'G
Cuts at: 830 2726 2994 3629 830
Size: 1896 268 635 3478
Fragments arranged by size:
3478 1896 635 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2506 2995 3080 3317 3417 3494 2506
Size: 489 85 237 100 77 5289
Fragments arranged by size:
5289 489 237 100 85 77
SacI G_AGCT'C
Cuts at: 2547 2988 2547
Size: 441 5836
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SexAI A'CCwGG_T
Cuts at: 2556 2556
Size: 6277
SfcI C'TryA_G
Cuts at: 826 1045 2722 2990 3625 4760 5441 5632 826
Size: 219 1677 268 635 1135 681 191 1471
Fragments arranged by size:
1677 1471 1135 681 635 268 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4381 5282 5544 820
Size: 197 926 2438 901 262 1553
Fragments arranged by size:
2438 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6277
StyI C'CwwG_G
Cuts at: 513 1065 2463 2638 2953 3716 513
Size: 552 1398 175 315 763 3074
Fragments arranged by size:
3074 1398 763 552 315 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2876 3517 4304 4321 4474 4659
Size: 1255 498 798 641 787 17 153 185
Cuts at: 4659 5104 325
Size: 445 1498
Fragments arranged by size:
1498 1255 798 787 641 498 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4523 6235 96
Size: 274 80 33 51 1443 2546 1712 138
Fragments arranged by size:
2546 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2074 2106 2951 2983 6237 6269 2074
Size: 32 845 32 3254 32 2082
Fragments arranged by size:
3254 2082 845 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2567 1316
Size: 1251 5026
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3367 2011
Size: 1356 4921
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 4406 4406
Size: 6277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI