Restriction map for
pFN21AB9740
(Circular) (Six-base) MAPSORT of: pFN21AB9740.seq Check: 3523 from: 1 to: 6256
pFN21AB9740 6256 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2437 3433 2437
Size: 996 5260
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3219 3599 1937
Size: 1282 380 4594
Fragments arranged by size:
4594 1282 380
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 4319 5703 5971 1632
Size: 1010 1677 1384 268 1917
Fragments arranged by size:
1917 1677 1384 1010 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 2134 4985 2134
Size: 2851 3405
AleI CACnn'nnGTG
Cuts at: 1166 2156 2235 3002 3254 1166
Size: 990 79 767 252 4168
Fragments arranged by size:
4168 990 767 252 79
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 2140 3055 3520 3999 5571 2140
Size: 915 465 479 1572 2825
Fragments arranged by size:
2825 1572 915 479 465
ApaI G_GGCC'C
Cuts at: 2339 2339
Size: 6256
ApaLI G'TGCA_C
Cuts at: 3525 4313 5469 3525
Size: 788 1156 4312
Fragments arranged by size:
4312 1156 788
ApoI r'AATT_y
Cuts at: 1493 3565 3791 3943 6052 1493
Size: 2072 226 152 2109 1697
Fragments arranged by size:
2109 2072 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2316 2458 3271 3409 3581 1870
Size: 73 180 193 142 813 138 172 4545
Fragments arranged by size:
4545 813 193 180 172 142 138 73
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2237 2462 2949 3350 3497
Size: 325 217 627 450 225 487 401 147
Cuts at: 3497 3577 5032 618
Size: 80 1455 1842
Fragments arranged by size:
1842 1455 627 487 450 401 325 225
217 147 80
BanII G_rGCy'C
Cuts at: 1391 2339 2812 3214 3575 1391
Size: 948 473 402 361 4072
Fragments arranged by size:
4072 948 473 402 361
BbeI G_GCGC'C
Cuts at: 2953 3354 2953
Size: 401 5855
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5285
BbvCI CC'TCA_GC
Cuts at: 3173 3173
Size: 6256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4447 4481 1903
Size: 34 2510 34 3678
Fragments arranged by size:
3678 2510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2304 2493 4867
Size: 107 122 71 631 605 632 189 2374
Cuts at: 4867 136
Size: 1525
Fragments arranged by size:
2374 1525 632 631 605 189 122 107
71
BglII A'GATC_T
Cuts at: 2658 6251 2658
Size: 3593 2663
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 2339 2467 2477 3500 3529 4317 5473 1790
Size: 549 128 10 1023 29 788 1156 2573
Fragments arranged by size:
2573 1156 1023 788 549 128 29 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3356 3508 3987
Size: 416 53 83 399 24 1939 152 479
Cuts at: 3987 4945 442
Size: 958 1753
Fragments arranged by size:
1939 1753 958 479 416 399 152 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3105 3114 4916 1239
Size: 1866 9 1802 2579
Fragments arranged by size:
2579 1866 1802 9
Bpu10I CC'TnA_GC
Cuts at: 3173 3173
Size: 6256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2538 4381 5246 5544 2538
Size: 1843 865 298 3250
Fragments arranged by size:
3250 1843 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3458 4919 882
Size: 617 1959 1461 2219
Fragments arranged by size:
2219 1959 1461 617
BsaAI yAC'GTr
Cuts at: 493 1204 2536 4047 493
Size: 711 1332 1511 2702
Fragments arranged by size:
2702 1511 1332 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2950 3351 4063 4445
Size: 53 83 186 936 1417 401 712 382
Cuts at: 4445 5080 275
Size: 635 1451
Fragments arranged by size:
1451 1417 936 712 635 401 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2886 3475 4689 5087 5361 5508 5919 2886
Size: 589 1214 398 274 147 411 3223
Fragments arranged by size:
3223 1214 589 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6031
Fragments arranged by size:
6031 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2776 2855 2858 2921 3326 3496 3499 1186
Size: 1590 79 3 63 405 170 3 3943
Fragments arranged by size:
3943 1590 405 170 79 63 3 3
BseYI C'CCAG_C
Cuts at: 2246 2299 2466 2526 3036 3048 3179 5459 5955
Size: 53 167 60 510 12 131 2280 496
Cuts at: 5955 2246
Size: 2547
Fragments arranged by size:
2547 2280 510 496 167 131 60 53
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2546 3071 3140 1163
Size: 573 810 525 69 4279
Fragments arranged by size:
4279 810 573 525 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3706 4467 5495 1540
Size: 275 186 931 774 761 1028 2301
Fragments arranged by size:
2301 1028 931 774 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2682 3529 4317 4402 5473 2682
Size: 847 788 85 1071 3465
Fragments arranged by size:
3465 1071 847 788 85
BsmI GAATG_Cn'
Cuts at: 2545 3767 3860 2545
Size: 1222 93 4941
Fragments arranged by size:
4941 1222 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2339 2467 2477 2682 2812 3214 3500
Size: 399 549 128 10 205 130 402 286
Cuts at: 3500 3529 3575 4317 4402 5473 1391
Size: 29 46 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 549 402 399 286 205
130 128 85 46 29 10
BspEI T'CCGG_A
Cuts at: 3475 5919 3475
Size: 2444 3812
BspHI T'CATG_A
Cuts at: 2670 4145 5875 2670
Size: 1475 1730 3051
Fragments arranged by size:
3051 1730 1475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2268 2437 3433 3611 844
Size: 1295 129 169 996 178 3489
Fragments arranged by size:
3489 1295 996 178 169 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2452 2781 3187 4143 1394
Size: 429 629 329 406 956 3507
Fragments arranged by size:
3507 956 629 429 406 329
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 2946 4900 5087 1700
Size: 110 1121 15 1954 187 2869
Fragments arranged by size:
2869 1954 1121 187 110 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2799 3116 3163 794
Size: 2005 317 47 3887
Fragments arranged by size:
3887 2005 317 47
BstBI TT'CG_AA
Cuts at: 2047 5060 2047
Size: 3013 3243
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstXI CCAn_nnnn'nTGG
Cuts at: 3004 3004
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 2658 3586 3953 4338 4355 5807 5893 5905
Size: 686 928 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 928 686 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2904 3391 2904
Size: 487 5769
BtgI C'CryG_G
Cuts at: 513 1065 2293 2376 2613 3009 3201 513
Size: 552 1228 83 237 396 192 3568
Fragments arranged by size:
3568 1228 552 396 237 192 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2124 487
Size: 683 56 898 4619
Fragments arranged by size:
4619 898 683 56
BtsI GCAGTG_nn'
Cuts at: 2477 3152 3772 4566 4586 2477
Size: 675 620 794 20 4147
Fragments arranged by size:
4147 794 675 620 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 2253 2347
Size: 54 1425 50 275 18 361 62 94
Cuts at: 2347 2895 3006 3703 4592 6221 8
Size: 548 111 697 889 1629 43
Fragments arranged by size:
1629 1425 889 697 548 361 275 111
94 62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2855 4186 1353
Size: 1502 1331 3423
Fragments arranged by size:
3423 1502 1331
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2642 3105 3114 4319 4916 5703 5971
Size: 393 1010 463 9 1205 597 787 268
Cuts at: 5971 1239
Size: 1524
Fragments arranged by size:
1524 1205 1010 787 597 463 393 268
9
EcoNI CCTnn'n_nnAGG
Cuts at: 3308 3308
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 2128 2336 2407 2869 2977 3387 2128
Size: 208 71 462 108 410 4997
Fragments arranged by size:
4997 462 410 208 108 71
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 6256
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2953 3354 5403 1678
Size: 1275 401 2049 2531
Fragments arranged by size:
2531 2049 1275 401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2120 2152 4897
Size: 32 135 32 437 32 79 32 2745
Cuts at: 4897 4929 4971 5003 1373
Size: 32 42 32 2626
Fragments arranged by size:
2745 2626 437 135 79 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3600 3846 669
Size: 988 281 1662 246 3079
Fragments arranged by size:
3079 1662 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 2949 3350 2949
Size: 401 5855
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2892 2952 5370 5554 1305
Size: 1587 60 2418 184 2007
Fragments arranged by size:
2418 2007 1587 184 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2349 2897 10
Size: 54 1425 343 517 548 3369
Fragments arranged by size:
3369 1425 548 517 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 2235 3002 3242 3254 4214 4573
Size: 648 990 79 767 240 12 960 359
Cuts at: 4573 4732 518
Size: 159 2042
Fragments arranged by size:
2042 990 960 767 648 359 240 159
79 12
MspA1I CmG'CkG
Cuts at: 1606 2601 3672 4349 5497 5742 1606
Size: 995 1071 677 1148 245 2120
Fragments arranged by size:
2120 1148 1071 995 677 245
NaeI GCC'GGC
Cuts at: 2948 2948
Size: 6256
NarI GG'CG_CC
Cuts at: 2950 3351 2950
Size: 401 5855
NcoI C'CATG_G
Cuts at: 513 1065 2293 2613 3201 513
Size: 552 1228 320 588 3568
Fragments arranged by size:
3568 1228 588 552 320
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NgoMIV G'CCGG_C
Cuts at: 2946 2946
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2267 2326 3614 5159 1132
Size: 97 1038 59 1288 1545 2229
Fragments arranged by size:
2229 1545 1288 1038 97 59
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3520 1108
Size: 2412 3844
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PmlI CAC'GTG
Cuts at: 2536 2536
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2407 2869 2128
Size: 279 462 5515
Fragments arranged by size:
5515 462 279
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 2335 2335
Size: 6256
PstI C_TGCA'G
Cuts at: 830 2136 2178 2406 3075 3297 3608 830
Size: 1306 42 228 669 222 311 3478
Fragments arranged by size:
3478 1306 669 311 228 222 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2601 2601
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SbfI CC_TGCA'GG
Cuts at: 2406 3608 2406
Size: 1202 5054
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SexAI A'CCwGG_T
Cuts at: 3093 3093
Size: 6256
SfcI C'TryA_G
Cuts at: 826 1045 2132 2174 2402 2662 3071 3216 3293
Size: 219 1087 42 228 260 409 145 77
Cuts at: 3293 3543 3604 4739 5420 5611 826
Size: 250 61 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 681 409 260 250 228
219 191 145 77 61 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 2493 2493
Size: 6256
SfoI GGC'GCC
Cuts at: 2951 3352 2951
Size: 401 5855
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 2125 2460 3583 1872
Size: 253 335 1123 4545
Fragments arranged by size:
4545 1123 335 253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2553 4360 5261 5523 820
Size: 197 926 610 1807 901 262 1553
Fragments arranged by size:
1807 1553 926 901 610 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 2326 3614 1132
Size: 1194 1288 3774
Fragments arranged by size:
3774 1288 1194
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 3396 1517
Size: 1879 4377
StyI C'CwwG_G
Cuts at: 513 1065 2241 2293 2613 3201 3695 513
Size: 552 1176 52 320 588 494 3074
Fragments arranged by size:
3074 1176 588 552 494 320 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2195 2247 2450 3512 4283 4300 4453
Size: 1255 615 52 203 1062 771 17 153
Cuts at: 4453 4638 5083 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1062 771 615 445 203 185
153 52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 4502 6214
Size: 274 80 33 51 1443 444 2081 1712
Cuts at: 6214 96
Size: 138
Fragments arranged by size:
2081 1712 1443 444 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2266 2298 6216 6248 2266
Size: 32 3918 32 2274
Fragments arranged by size:
3918 2274 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3064 1316
Size: 1748 4508
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 3007 2393
Size: 614 5642
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 2123 2458 3581 1870
Size: 253 335 1123 4545
Fragments arranged by size:
4545 1123 335 253
XmnI GAAnn'nnTTC
Cuts at: 4385 4385
Size: 6256
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SacII
SanDI SapI SrfI SwaI