Restriction map for
pFN21AB9737
(Circular) (Six-base) MAPSORT of: pFN21AB9737.seq Check: 4863 from: 1 to: 6205
pFN21AB9737 6205 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2623 2623
Size: 6205
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3548 1937
Size: 1611 4594
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2531 3308 4268 5652 5920 1632
Size: 899 777 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 899 777 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3197 1184
Size: 2013 4192
AlwNI CAG_nnn'CTG
Cuts at: 3100 3109 3948 5520 3100
Size: 9 839 1572 3785
Fragments arranged by size:
3785 1572 839 9
ApaLI G'TGCA_C
Cuts at: 4262 5418 4262
Size: 1156 5049
ApoI r'AATT_y
Cuts at: 1493 2604 3514 3740 3892 6001 1493
Size: 1111 910 226 152 2109 1697
Fragments arranged by size:
2109 1697 1111 910 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 3530 1870
Size: 73 134 1453 4545
Fragments arranged by size:
4545 1453 134 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2978 3011 2978
Size: 33 6172
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2153 2195 2210 3526
Size: 325 217 627 235 131 42 15 1316
Cuts at: 3526 4981 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 235 217 131
42 15
BanII G_rGCy'C
Cuts at: 1391 2033 2964 3162 3524 1391
Size: 642 931 198 362 4072
Fragments arranged by size:
4072 931 642 362 198
BbeI G_GCGC'C
Cuts at: 2199 2199
Size: 6205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5234
BbvCI CC'TCA_GC
Cuts at: 2093 2910 2093
Size: 817 5388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2222 2256 4396 4430 1903
Size: 34 285 34 2140 34 3678
Fragments arranged by size:
3678 2140 285 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4097 5307 3446
Size: 651 1210 4344
Fragments arranged by size:
4344 1210 651
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2112 3218 4816
Size: 107 122 71 631 605 440 1106 1598
Cuts at: 4816 136
Size: 1525
Fragments arranged by size:
1598 1525 1106 631 605 440 122 107
71
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 2661 3140 3622 2661
Size: 479 482 5244
Fragments arranged by size:
5244 482 479
Bme1580I G_kGCm'C
Cuts at: 1790 2156 3264 3354 3440 4266 5422 1790
Size: 366 1108 90 86 826 1156 2573
Fragments arranged by size:
2573 1156 1108 826 366 90 86
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2103 3936 4894
Size: 416 53 83 399 24 686 1833 958
Cuts at: 4894 442
Size: 1753
Fragments arranged by size:
1833 1753 958 686 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2017 2978 3184 3302 3311 3362 3482 4865
Size: 778 961 206 118 9 51 120 1383
Cuts at: 4865 1239
Size: 2579
Fragments arranged by size:
2579 1383 961 778 206 120 118 51
9
Bpu10I CC'TnA_GC
Cuts at: 2093 2594 2910 3444 2093
Size: 501 316 534 4854
Fragments arranged by size:
4854 534 501 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2782 2990 3020 4330 5195 5493 2782
Size: 208 30 1310 865 298 3494
Fragments arranged by size:
3494 1310 865 298 208 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4868 882
Size: 617 3369 2219
Fragments arranged by size:
3369 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 2849 6199 2849
Size: 3350 2855
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2196 4012 4394
Size: 53 83 186 936 591 72 1816 382
Cuts at: 4394 5029 275
Size: 635 1451
Fragments arranged by size:
1816 1451 936 635 591 382 186 83
72 53
BsaWI w'CCGG_w
Cuts at: 4638 5036 5310 5457 5868 4638
Size: 398 274 147 411 4975
Fragments arranged by size:
4975 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 1178
Size: 30 165 30 612 30 5338
Fragments arranged by size:
5338 612 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2945 3035 3170 1186
Size: 1759 90 135 4221
Fragments arranged by size:
4221 1759 135 90
BseYI C'CCAG_C
Cuts at: 2203 2772 5408 5904 2203
Size: 569 2636 496 2504
Fragments arranged by size:
2636 2504 569 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3098 3245 1163
Size: 573 1362 147 4123
Fragments arranged by size:
4123 1362 573 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3213 3655 4416 5444 1540
Size: 275 186 1212 442 761 1028 2301
Fragments arranged by size:
2301 1212 1028 761 442 275 186
BsiHKAI G_wGCw'C
Cuts at: 2937 2964 4266 4351 5422 2937
Size: 27 1302 85 1071 3720
Fragments arranged by size:
3720 1302 1071 85 27
BsmI GAATG_Cn'
Cuts at: 3716 3809 3716
Size: 93 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2156 2937 2964 3162 3264 3354
Size: 399 243 123 781 27 198 102 90
Cuts at: 3354 3440 3524 4266 4351 5422 1391
Size: 86 84 742 85 1071 2174
Fragments arranged by size:
2174 1071 781 742 399 243 198 123
102 90 86 85 84 27
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 2484 4094 5824 2484
Size: 1610 1730 2865
Fragments arranged by size:
2865 1730 1610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2623 2982 3117 3476 3560 844
Size: 1779 359 135 359 84 3489
Fragments arranged by size:
3489 1779 359 359 135 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2877 4092 1394
Size: 429 1054 1215 3507
Fragments arranged by size:
3507 1215 1054 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2328 4700 4874 65
Size: 1183 1080 2372 174 1396
Fragments arranged by size:
2372 1396 1183 1080 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4849 5036 1700
Size: 110 3039 187 2869
Fragments arranged by size:
3039 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3069 96
Size: 2973 3232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 2315 3116 4265 5277 2315
Size: 801 1149 1012 3243
Fragments arranged by size:
3243 1149 1012 801
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2217 2466 3109 3388 794
Size: 1423 249 643 279 3611
Fragments arranged by size:
3611 1423 643 279 249
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstXI CCAn_nnnn'nTGG
Cuts at: 2170 2170
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 3079 3535 3902 4287 4304 5756 5842 5854
Size: 1107 456 367 385 17 1452 86 12
Cuts at: 5854 6200 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1107 456 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2703 2703
Size: 6205
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 3122 3188 3438 3721 4515 4535 3122
Size: 66 250 283 794 20 4792
Fragments arranged by size:
4792 794 283 250 66 20
ClaI AT'CG_AT
Cuts at: 1554 2085 1554
Size: 531 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2062 5212 809
Size: 507 746 3150 1802
Fragments arranged by size:
3150 1802 746 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 2424 3210
Size: 54 1425 50 275 18 435 159 786
Cuts at: 3210 3491 3652 4541 6170 8
Size: 281 161 889 1629 43
Fragments arranged by size:
1629 1425 889 786 435 281 275 161
159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3210 3652 1537
Size: 275 1398 442 4090
Fragments arranged by size:
4090 1398 442 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3281 4135 1353
Size: 1928 854 3423
Fragments arranged by size:
3423 1928 854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2962 2962
Size: 6205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2531 2978 3184 3302 3308 3311
Size: 393 385 514 447 206 118 6 3
Cuts at: 3311 3362 3482 4268 4865 5652 5920 1239
Size: 51 120 786 597 787 268 1524
Fragments arranged by size:
1524 787 786 597 514 447 393 385
268 206 120 118 51 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3307 3307
Size: 6205
EcoO109I rG'GnC_Cy
Cuts at: 2719 3040 3106 2719
Size: 321 66 5818
Fragments arranged by size:
5818 321 66
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 6205
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2199 2308 3151 5352 1678
Size: 521 109 843 2201 2531
Fragments arranged by size:
2531 2201 843 521 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 4846 4878 4920
Size: 32 135 32 746 32 2496 32 42
Cuts at: 4920 4952 1373
Size: 32 2626
Fragments arranged by size:
2626 2496 746 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3549 3795 669
Size: 988 281 1611 246 3079
Fragments arranged by size:
3079 1611 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KasI G'GCGC_C
Cuts at: 2195 2195
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 5319 5503 1305
Size: 840 3174 184 2007
Fragments arranged by size:
3174 2007 840 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3493 10
Size: 54 1425 343 1661 2722
Fragments arranged by size:
2722 1661 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4163 4522 4681 518
Size: 648 2997 359 159 2042
Fragments arranged by size:
2997 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2130 2247 2418 2983 3196 3503 3621
Size: 431 93 117 171 565 213 307 118
Cuts at: 3621 4298 5446 5691 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 431 307 245 213
171 118 117 93
NarI GG'CG_CC
Cuts at: 2196 2196
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5653
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 2287 3389 3563 5108 1132
Size: 97 1058 1102 174 1545 2229
Fragments arranged by size:
2229 1545 1102 1058 174 97
PasI CC'CwG_GG
Cuts at: 1348 2149 2809 1348
Size: 801 660 4744
Fragments arranged by size:
4744 801 660
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3303 1108
Size: 2195 4010
PfoI T'CCnGG_A
Cuts at: 3279 3279
Size: 6205
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3106 3106
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2396 2428 2396
Size: 32 6173
PstI C_TGCA'G
Cuts at: 830 2835 2982 3105 3129 3252 3557 830
Size: 2005 147 123 24 123 305 3478
Fragments arranged by size:
3478 2005 305 147 123 123 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 2037 2418 2983 3503 2037
Size: 381 565 520 4739
Fragments arranged by size:
4739 565 520 381
SacI G_AGCT'C
Cuts at: 2964 2964
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 2831 2978 3101 3125 3235 3248 3553
Size: 219 1786 147 123 24 110 13 305
Cuts at: 3553 4688 5369 5560 826
Size: 1135 681 191 1471
Fragments arranged by size:
1786 1471 1135 681 305 219 191 147
123 110 24 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 3218 3218
Size: 6205
SfoI GGC'GCC
Cuts at: 2197 2197
Size: 6205
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2797 3005 3035 4309 5210 5472
Size: 197 926 854 208 30 1274 901 262
Cuts at: 5472 820
Size: 1553
Fragments arranged by size:
1553 1274 926 901 854 262 208 197
30
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 2378 2474 3302 1517
Size: 861 96 828 4420
Fragments arranged by size:
4420 861 828 96
StyI C'CwwG_G
Cuts at: 513 1065 2398 3216 3297 3644 513
Size: 552 1333 818 81 347 3074
Fragments arranged by size:
3074 1333 818 552 347 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 2197 2763 3421 4232 4249 4402
Size: 1255 591 26 566 658 811 17 153
Cuts at: 4402 4587 5032 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 811 658 591 566 445 185
153 26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 2412 3069
Size: 274 80 33 51 1443 303 132 657
Cuts at: 3069 4451 6163 96
Size: 1382 1712 138
Fragments arranged by size:
1712 1443 1382 657 303 274 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2015 2047 6165 6197 2015
Size: 32 4118 32 2023
Fragments arranged by size:
4118 2023 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3082 3541 3082
Size: 459 5746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2251 2980 3426 2108
Size: 143 729 446 4887
Fragments arranged by size:
4887 729 446 143
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI
SapI SexAI SrfI SwaI