Restriction map for
pFN21AB9734
(Circular) (Six-base) MAPSORT of: pFN21AB9734.seq Check: 2241 from: 1 to: 6163
pFN21AB9734 6163 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3213 3213
Size: 6163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2550 3973 278
Size: 53 83 186 936 1014 1423 2468
Fragments arranged by size:
2468 1423 1014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3506 1937
Size: 1569 4594
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 3244 4226 5610 5878 1632
Size: 447 1165 982 1384 268 1917
Fragments arranged by size:
1917 1384 1165 982 447 268
AfeI AGC'GCT
Cuts at: 2083 2586 2083
Size: 503 5660
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 3327 5062 6039 1203
Size: 2124 1735 977 1327
Fragments arranged by size:
2124 1735 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 2734 4892 2734
Size: 2158 4005
AleI CACnn'nnGTG
Cuts at: 1166 3287 1166
Size: 2121 4042
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AlwNI CAG_nnn'CTG
Cuts at: 2518 3906 5478 2518
Size: 1388 1572 3203
Fragments arranged by size:
3203 1572 1388
ApaI G_GGCC'C
Cuts at: 2650 2650
Size: 6163
ApaLI G'TGCA_C
Cuts at: 3380 4220 5376 3380
Size: 840 1156 4167
Fragments arranged by size:
4167 1156 840
ApoI r'AATT_y
Cuts at: 1493 3472 3698 3850 5959 1493
Size: 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2821 3488 1870
Size: 73 878 667 4545
Fragments arranged by size:
4545 878 667 73
BamHI G'GATC_C
Cuts at: 3493 3860 3493
Size: 367 5796
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2338 2501 2566 3084 3484
Size: 325 217 627 551 163 65 518 400
Cuts at: 3484 4939 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 551 518 400 325 217
163 65
BanII G_rGCy'C
Cuts at: 1391 2650 2862 3195 3482 1391
Size: 1259 212 333 287 4072
Fragments arranged by size:
4072 1259 333 287 212
BbeI G_GCGC'C
Cuts at: 2570 3088 2570
Size: 518 5645
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5192
BbvCI CC'TCA_GC
Cuts at: 2243 2529 2844 2931 2243
Size: 286 315 87 5475
Fragments arranged by size:
5475 315 286 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4354 4388 1903
Size: 34 50 34 2333 34 3678
Fragments arranged by size:
3678 2333 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2693 2966 3093
Size: 107 122 71 631 605 1021 273 127
Cuts at: 3093 4774 136
Size: 1681 1525
Fragments arranged by size:
1681 1525 1021 631 605 273 127 122
107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3580 3580
Size: 6163
Bme1580I G_kGCm'C
Cuts at: 1790 2280 2506 2650 3384 4224 5380 1790
Size: 490 226 144 734 840 1156 2573
Fragments arranged by size:
2573 1156 840 734 490 226 144
BmgBI CAC'GTC
Cuts at: 1318 3328 1318
Size: 2010 4153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3894 4852 442
Size: 416 53 83 399 24 2477 958 1753
Fragments arranged by size:
2477 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2231 4823 1239
Size: 918 74 2592 2579
Fragments arranged by size:
2592 2579 918 74
Bpu10I CC'TnA_GC
Cuts at: 2015 2243 2529 2844 2931 2015
Size: 228 286 315 87 5247
Fragments arranged by size:
5247 315 286 228 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 3371 3401 4288 5153 5451 2173
Size: 1198 30 887 865 298 2885
Fragments arranged by size:
2885 1198 887 865 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3289 3954 493
Size: 711 2085 665 2702
Fragments arranged by size:
2702 2085 711 665
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2547 2567 3085 3970
Size: 53 83 186 936 1014 20 518 885
Cuts at: 3970 4352 4987 275
Size: 382 635 1451
Fragments arranged by size:
1451 1014 936 885 635 518 382 186
83 53 20
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2218 2248 2423 2453 1178
Size: 30 165 30 815 30 175 30 4888
Fragments arranged by size:
4888 815 175 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 3422 1186
Size: 847 1389 3927
Fragments arranged by size:
3927 1389 847
BseYI C'CCAG_C
Cuts at: 2078 2157 2770 2789 2944 3439 5366 5862 2078
Size: 79 613 19 155 495 1927 496 2379
Fragments arranged by size:
2379 1927 613 496 495 155 79 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3006 1163
Size: 573 1270 4320
Fragments arranged by size:
4320 1270 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2766 3128 3337 3613 4374 5402
Size: 275 186 765 362 209 276 761 1028
Cuts at: 5402 1540
Size: 2301
Fragments arranged by size:
2301 1028 765 761 362 276 275 209
186
BsiHKAI G_wGCw'C
Cuts at: 3195 3384 4224 4309 5380 3195
Size: 189 840 85 1071 3978
Fragments arranged by size:
3978 1071 840 189 85
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2506 2650 2862 3195 3384 3482
Size: 399 490 226 144 212 333 189 98
Cuts at: 3482 4224 4309 5380 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 333 226 212
189 144 98 85
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3213 3518 844
Size: 2369 305 3489
Fragments arranged by size:
3489 2369 305
BsrBI CCG'CTC
Cuts at: 1394 1823 3412 4050 1394
Size: 429 1589 638 3507
Fragments arranged by size:
3507 1589 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4658 4832 65
Size: 1183 3410 174 1396
Fragments arranged by size:
3410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 2761 3302 4807 4994 1700
Size: 110 316 635 541 1505 187 2869
Fragments arranged by size:
2869 1505 635 541 316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 3179 3347 3398 3400 1925
Size: 1254 168 51 2 4688
Fragments arranged by size:
4688 1254 168 51 2
BssSI C'ACGA_G
Cuts at: 4223 5235 4223
Size: 1012 5151
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3111 794
Size: 2317 3846
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 2372 2446 3493 3860 4245 4262 5714 5800
Size: 400 74 1047 367 385 17 1452 86
Cuts at: 5800 5812 6158 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1047 400 385 367 346 86
74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2118 2964 3088 3122 513
Size: 552 1053 846 124 34 3554
Fragments arranged by size:
3554 1053 846 552 124 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3369 487
Size: 683 56 2143 3281
Fragments arranged by size:
3281 2143 683 56
BtsI GCAGTG_nn'
Cuts at: 2280 2519 3016 3679 4473 4493 2280
Size: 239 497 663 794 20 3950
Fragments arranged by size:
3950 794 663 497 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2763 2916
Size: 54 1425 50 275 18 291 642 153
Cuts at: 2916 3125 3334 3610 4499 6128 8
Size: 209 209 276 889 1629 43
Fragments arranged by size:
1629 1425 889 642 291 276 275 209
209 153 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2763 3125 3334 3610 1537
Size: 275 951 362 209 276 4090
Fragments arranged by size:
4090 951 362 276 275 209
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 4093 1353
Size: 959 1781 3423
Fragments arranged by size:
3423 1781 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2415 2919 2984 3008 4750 5124
Size: 1064 138 826 504 65 24 1742 374
Cuts at: 5124 5270 387
Size: 146 1280
Fragments arranged by size:
1742 1280 1064 826 504 374 146 138
65 24
EcoICRI GAG'CTC
Cuts at: 3193 3193
Size: 6163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2157 2231 3244 4226 4823 5610
Size: 393 447 78 74 1013 982 597 787
Cuts at: 5610 5878 1239
Size: 268 1524
Fragments arranged by size:
1524 1013 982 787 597 447 393 268
78 74
EcoNI CCTnn'n_nnAGG
Cuts at: 2139 2139
Size: 6163
EcoO109I rG'GnC_Cy
Cuts at: 2216 2612 2646 2647 2796 2848 3421 2216
Size: 396 34 1 149 52 573 4958
Fragments arranged by size:
4958 573 396 149 52 34 1
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FspI TGC'GCA
Cuts at: 3209 3293 4669 3209
Size: 84 1376 4703
Fragments arranged by size:
4703 1376 84
FspAI rTGC'GCAy
Cuts at: 3293 3293
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 2085 2457 2570 2588 2961 3088 5310 1678
Size: 407 372 113 18 373 127 2222 2531
Fragments arranged by size:
2531 2222 407 373 372 127 113 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 2582 2614 2720
Size: 32 135 32 851 32 127 32 106
Cuts at: 2720 2752 4804 4836 4878 4910 1373
Size: 32 2052 32 42 32 2626
Fragments arranged by size:
2626 2052 851 135 127 106 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3507 3753 669
Size: 988 281 1569 246 3079
Fragments arranged by size:
3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KasI G'GCGC_C
Cuts at: 2566 3084 2566
Size: 518 5645
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3210 5277 5461 1305
Size: 1905 2067 184 2007
Fragments arranged by size:
2067 2007 1905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2918 10
Size: 54 1425 343 1086 3255
Fragments arranged by size:
3255 1425 1086 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3287 4121 4480 4639 518
Size: 648 2121 834 359 159 2042
Fragments arranged by size:
2121 2042 834 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2172 2253 2380 2745 2793 2815 2944
Size: 514 52 81 127 365 48 22 129
Cuts at: 2944 2966 3090 3124 3579 4256 5404 5649 1606
Size: 22 124 34 455 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 514 455 365 245 129
127 124 81 52 48 34 22 22
NaeI GCC'GGC
Cuts at: 2763 2763
Size: 6163
NarI GG'CG_CC
Cuts at: 2567 3085 2567
Size: 518 5645
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5611
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NgoMIV G'CCGG_C
Cuts at: 2761 2761
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3125 3334 3610 3125
Size: 209 276 5678
Fragments arranged by size:
5678 276 209
NspI r_CATG'y
Cuts at: 1132 1229 3521 5066 1132
Size: 97 2292 1545 2229
Fragments arranged by size:
2292 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2625 2651 2855 1348
Size: 1277 26 204 4656
Fragments arranged by size:
4656 1277 204 26
PciI A'CATG_T
Cuts at: 5062 5062
Size: 6163
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6163
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 6163
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PmlI CAC'GTG
Cuts at: 3289 3289
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2612 2612
Size: 6163
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PspOMI G'GGCC_C
Cuts at: 2646 2646
Size: 6163
PstI C_TGCA'G
Cuts at: 830 3515 830
Size: 2685 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
PvuII CAG'CTG
Cuts at: 2172 2745 2172
Size: 573 5590
SacI G_AGCT'C
Cuts at: 3195 3195
Size: 6163
SacII CC_GC'GG
Cuts at: 2121 2967 3091 3125 2121
Size: 846 124 34 5159
Fragments arranged by size:
5159 846 124 34
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SanDI GG'GwC_CC
Cuts at: 2612 2612
Size: 6163
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 2248 3511 4646 5327 5518 826
Size: 219 1203 1263 1135 681 191 1471
Fragments arranged by size:
1471 1263 1203 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2568 3086 2568
Size: 518 5645
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 3302 1700
Size: 1602 4561
SmaI CCC'GGG
Cuts at: 1872 2823 3490 1872
Size: 951 667 4545
Fragments arranged by size:
4545 951 667
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2152 3386 3416 4267 5168 5430
Size: 197 926 209 1234 30 851 901 262
Cuts at: 5430 820
Size: 1553
Fragments arranged by size:
1553 1234 926 901 851 262 209 197
30
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 2334 2486 3140 1517
Size: 817 152 654 4540
Fragments arranged by size:
4540 817 654 152
StyI C'CwwG_G
Cuts at: 513 1065 3066 3135 3602 513
Size: 552 2001 69 467 3074
Fragments arranged by size:
3074 2001 552 467 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3311 4190 4207 4360 4545 4990 325
Size: 1255 1731 879 17 153 185 445 1498
Fragments arranged by size:
1731 1498 1255 879 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4409 6121 96
Size: 274 80 33 51 1443 2432 1712 138
Fragments arranged by size:
2432 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2218 2250 6123 6155 2218
Size: 32 3873 32 2226
Fragments arranged by size:
3873 2226 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2594 1316
Size: 1278 4885
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2475 2377
Size: 98 6065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 2821 3488 1870
Size: 951 667 4545
Fragments arranged by size:
4545 951 667
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2548 3971 276
Size: 53 83 186 936 1014 1423 2468
Fragments arranged by size:
2468 1423 1014 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI NruI
NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI
SrfI SwaI